Simultaneous purification and immobilization of soybean hull peroxidase with a dye attached to chitosan mini-spheres
- Autores
- Bracco, Lautaro Fidel; Levin, Gustavo Javier; Navarro, Agustin Andres; Wolman, Federico Javier; Miranda, Maria Victoria; Cascone, Osvaldo
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Soybean hull peroxidase (EC 1.11.1.7, SBP) was simultaneously purified and immobilized by dye affinity chromatography with Reactive Blue 4 attached to chitosan mini-spheres. Under optimized conditions, 96% of SBP was adsorbed to the matrix. Under the most stringent condition, only 49% was desorbed, whereas 2 M NaCl failed to desorb a significant amount of SBP. This behaviour allowed proposing the dye matrix as a support to immobilize SBP from a crude extract. The pH of maximum activity shifted from 7 to 3?5. SBP gained thermostability after immobilization: after 5 h at 85 °C, the remaining activity was 54%, whereas that of the free enzyme was 31%. The optimum temperature for the immobilized SBP was 75 °C, whereas that of the free enzyme was 55 °C. After two months at 4 °C, the activity loss of the immobilized SBP was only 3%. Immobilized SBP removed 80% of 2-bromophenol from wastewater in 180 min and, after five cycles of use, the activity loss was only 12.8%.
Fil: Bracco, Lautaro Fidel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina
Fil: Levin, Gustavo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Rios. Universidad Nacional de Entre Rios. Centro de Investigaciones y Transferencia de Entre Rios; Argentina
Fil: Navarro, Agustin Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina
Fil: Wolman, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina
Fil: Miranda, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina
Fil: Cascone, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina - Materia
-
Soybean Peroxidase
Purification
Immobilization
Affinity Chromatography - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/41007
Ver los metadatos del registro completo
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Simultaneous purification and immobilization of soybean hull peroxidase with a dye attached to chitosan mini-spheresBracco, Lautaro FidelLevin, Gustavo JavierNavarro, Agustin AndresWolman, Federico JavierMiranda, Maria VictoriaCascone, OsvaldoSoybean PeroxidasePurificationImmobilizationAffinity Chromatographyhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Soybean hull peroxidase (EC 1.11.1.7, SBP) was simultaneously purified and immobilized by dye affinity chromatography with Reactive Blue 4 attached to chitosan mini-spheres. Under optimized conditions, 96% of SBP was adsorbed to the matrix. Under the most stringent condition, only 49% was desorbed, whereas 2 M NaCl failed to desorb a significant amount of SBP. This behaviour allowed proposing the dye matrix as a support to immobilize SBP from a crude extract. The pH of maximum activity shifted from 7 to 3?5. SBP gained thermostability after immobilization: after 5 h at 85 °C, the remaining activity was 54%, whereas that of the free enzyme was 31%. The optimum temperature for the immobilized SBP was 75 °C, whereas that of the free enzyme was 55 °C. After two months at 4 °C, the activity loss of the immobilized SBP was only 3%. Immobilized SBP removed 80% of 2-bromophenol from wastewater in 180 min and, after five cycles of use, the activity loss was only 12.8%.Fil: Bracco, Lautaro Fidel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Levin, Gustavo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Rios. Universidad Nacional de Entre Rios. Centro de Investigaciones y Transferencia de Entre Rios; ArgentinaFil: Navarro, Agustin Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Wolman, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Miranda, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Cascone, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaTaylor & Francis Ltd2017-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41007Bracco, Lautaro Fidel; Levin, Gustavo Javier; Navarro, Agustin Andres; Wolman, Federico Javier; Miranda, Maria Victoria; et al.; Simultaneous purification and immobilization of soybean hull peroxidase with a dye attached to chitosan mini-spheres; Taylor & Francis Ltd; Biocatalysis and Biotransformation; 35; 5; 6-2017; 1-91024-2422CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/10242422.2017.1334767info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/10242422.2017.1334767info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:00:25Zoai:ri.conicet.gov.ar:11336/41007instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:00:25.423CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Simultaneous purification and immobilization of soybean hull peroxidase with a dye attached to chitosan mini-spheres |
| title |
Simultaneous purification and immobilization of soybean hull peroxidase with a dye attached to chitosan mini-spheres |
| spellingShingle |
Simultaneous purification and immobilization of soybean hull peroxidase with a dye attached to chitosan mini-spheres Bracco, Lautaro Fidel Soybean Peroxidase Purification Immobilization Affinity Chromatography |
| title_short |
Simultaneous purification and immobilization of soybean hull peroxidase with a dye attached to chitosan mini-spheres |
| title_full |
Simultaneous purification and immobilization of soybean hull peroxidase with a dye attached to chitosan mini-spheres |
| title_fullStr |
Simultaneous purification and immobilization of soybean hull peroxidase with a dye attached to chitosan mini-spheres |
| title_full_unstemmed |
Simultaneous purification and immobilization of soybean hull peroxidase with a dye attached to chitosan mini-spheres |
| title_sort |
Simultaneous purification and immobilization of soybean hull peroxidase with a dye attached to chitosan mini-spheres |
| dc.creator.none.fl_str_mv |
Bracco, Lautaro Fidel Levin, Gustavo Javier Navarro, Agustin Andres Wolman, Federico Javier Miranda, Maria Victoria Cascone, Osvaldo |
| author |
Bracco, Lautaro Fidel |
| author_facet |
Bracco, Lautaro Fidel Levin, Gustavo Javier Navarro, Agustin Andres Wolman, Federico Javier Miranda, Maria Victoria Cascone, Osvaldo |
| author_role |
author |
| author2 |
Levin, Gustavo Javier Navarro, Agustin Andres Wolman, Federico Javier Miranda, Maria Victoria Cascone, Osvaldo |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
Soybean Peroxidase Purification Immobilization Affinity Chromatography |
| topic |
Soybean Peroxidase Purification Immobilization Affinity Chromatography |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
| dc.description.none.fl_txt_mv |
Soybean hull peroxidase (EC 1.11.1.7, SBP) was simultaneously purified and immobilized by dye affinity chromatography with Reactive Blue 4 attached to chitosan mini-spheres. Under optimized conditions, 96% of SBP was adsorbed to the matrix. Under the most stringent condition, only 49% was desorbed, whereas 2 M NaCl failed to desorb a significant amount of SBP. This behaviour allowed proposing the dye matrix as a support to immobilize SBP from a crude extract. The pH of maximum activity shifted from 7 to 3?5. SBP gained thermostability after immobilization: after 5 h at 85 °C, the remaining activity was 54%, whereas that of the free enzyme was 31%. The optimum temperature for the immobilized SBP was 75 °C, whereas that of the free enzyme was 55 °C. After two months at 4 °C, the activity loss of the immobilized SBP was only 3%. Immobilized SBP removed 80% of 2-bromophenol from wastewater in 180 min and, after five cycles of use, the activity loss was only 12.8%. Fil: Bracco, Lautaro Fidel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina Fil: Levin, Gustavo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Rios. Universidad Nacional de Entre Rios. Centro de Investigaciones y Transferencia de Entre Rios; Argentina Fil: Navarro, Agustin Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina Fil: Wolman, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina Fil: Miranda, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina Fil: Cascone, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina |
| description |
Soybean hull peroxidase (EC 1.11.1.7, SBP) was simultaneously purified and immobilized by dye affinity chromatography with Reactive Blue 4 attached to chitosan mini-spheres. Under optimized conditions, 96% of SBP was adsorbed to the matrix. Under the most stringent condition, only 49% was desorbed, whereas 2 M NaCl failed to desorb a significant amount of SBP. This behaviour allowed proposing the dye matrix as a support to immobilize SBP from a crude extract. The pH of maximum activity shifted from 7 to 3?5. SBP gained thermostability after immobilization: after 5 h at 85 °C, the remaining activity was 54%, whereas that of the free enzyme was 31%. The optimum temperature for the immobilized SBP was 75 °C, whereas that of the free enzyme was 55 °C. After two months at 4 °C, the activity loss of the immobilized SBP was only 3%. Immobilized SBP removed 80% of 2-bromophenol from wastewater in 180 min and, after five cycles of use, the activity loss was only 12.8%. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017-06 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/41007 Bracco, Lautaro Fidel; Levin, Gustavo Javier; Navarro, Agustin Andres; Wolman, Federico Javier; Miranda, Maria Victoria; et al.; Simultaneous purification and immobilization of soybean hull peroxidase with a dye attached to chitosan mini-spheres; Taylor & Francis Ltd; Biocatalysis and Biotransformation; 35; 5; 6-2017; 1-9 1024-2422 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/41007 |
| identifier_str_mv |
Bracco, Lautaro Fidel; Levin, Gustavo Javier; Navarro, Agustin Andres; Wolman, Federico Javier; Miranda, Maria Victoria; et al.; Simultaneous purification and immobilization of soybean hull peroxidase with a dye attached to chitosan mini-spheres; Taylor & Francis Ltd; Biocatalysis and Biotransformation; 35; 5; 6-2017; 1-9 1024-2422 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/altIdentifier/doi/10.1080/10242422.2017.1334767 info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/10242422.2017.1334767 |
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Taylor & Francis Ltd |
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Taylor & Francis Ltd |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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