The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth

Autores
Zhang, Dong; Wengier, Diego Leonardo; Shuai, Bin; Gui, Cai Ping; Muschietti, Jorge Prometeo; McCormick, Sheila; Tang, Wei Hua
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In flowering plants, the process of pollen germination and tube growth is required for successful fertilization. A pollen receptor kinase from tomato (Solanum lycopersicum), LePRK2, has been implicated in signaling during pollen germination and tube growth as well as in mediating pollen (tube)-pistil communication. Here we show that reduced expression of LePRK2 affects four aspects of pollen germination and tube growth. First, the percentage of pollen that germinates is reduced, and the time window for competence to germinate is also shorter. Second, the pollen tube growth rate is reduced both in vitro and in the pistil. Third, tip-localized superoxide production by pollen tubes cannot be increased by exogenous calcium ions. Fourth, pollen tubes have defects in responses to style extract component (STIL), an extracellular growth-promoting signal from the pistil. Pollen tubes transiently overexpressing LePRK2-fluorescent protein fusions had slightly wider tips, whereas pollen tubes coexpressing LePRK2 and its cytoplasmic partner protein KPP (a Rop-GEF) had much wider tips. Together these results show that LePRK2 positively regulates pollen germination and tube growth and is involved in transducing responses to extracellular growth-promoting signals.
Fil: Zhang, Dong. Chinese Academy of Sciences; República de China
Fil: Wengier, Diego Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; Argentina. University of California at Berkeley; Estados Unidos
Fil: Shuai, Bin. University of California at Berkeley; Estados Unidos
Fil: Gui, Cai Ping. Chinese Academy of Sciences; República de China
Fil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; Argentina
Fil: McCormick, Sheila. University of California at Berkeley; Estados Unidos
Fil: Tang, Wei Hua. Chinese Academy of Sciences; República de China. University of California at Berkeley; Estados Unidos
Materia
Pollen
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79639
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/79639
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growthZhang, DongWengier, Diego LeonardoShuai, BinGui, Cai PingMuschietti, Jorge PrometeoMcCormick, SheilaTang, Wei HuaPollenhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In flowering plants, the process of pollen germination and tube growth is required for successful fertilization. A pollen receptor kinase from tomato (Solanum lycopersicum), LePRK2, has been implicated in signaling during pollen germination and tube growth as well as in mediating pollen (tube)-pistil communication. Here we show that reduced expression of LePRK2 affects four aspects of pollen germination and tube growth. First, the percentage of pollen that germinates is reduced, and the time window for competence to germinate is also shorter. Second, the pollen tube growth rate is reduced both in vitro and in the pistil. Third, tip-localized superoxide production by pollen tubes cannot be increased by exogenous calcium ions. Fourth, pollen tubes have defects in responses to style extract component (STIL), an extracellular growth-promoting signal from the pistil. Pollen tubes transiently overexpressing LePRK2-fluorescent protein fusions had slightly wider tips, whereas pollen tubes coexpressing LePRK2 and its cytoplasmic partner protein KPP (a Rop-GEF) had much wider tips. Together these results show that LePRK2 positively regulates pollen germination and tube growth and is involved in transducing responses to extracellular growth-promoting signals.Fil: Zhang, Dong. Chinese Academy of Sciences; República de ChinaFil: Wengier, Diego Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; Argentina. University of California at Berkeley; Estados UnidosFil: Shuai, Bin. University of California at Berkeley; Estados UnidosFil: Gui, Cai Ping. Chinese Academy of Sciences; República de ChinaFil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; ArgentinaFil: McCormick, Sheila. University of California at Berkeley; Estados UnidosFil: Tang, Wei Hua. Chinese Academy of Sciences; República de China. University of California at Berkeley; Estados UnidosAmerican Society of Plant Biologist2008-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79639Zhang, Dong; Wengier, Diego Leonardo; Shuai, Bin; Gui, Cai Ping; Muschietti, Jorge Prometeo; et al.; The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth; American Society of Plant Biologist; Plant Physiology; 148; 3; 11-2008; 1368-13790032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/148/3/1368info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2577252/info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.108.124420info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:43Zoai:ri.conicet.gov.ar:11336/79639instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:43.434CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth
title The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth
spellingShingle The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth
Zhang, Dong
Pollen
title_short The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth
title_full The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth
title_fullStr The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth
title_full_unstemmed The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth
title_sort The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth
dc.creator.none.fl_str_mv Zhang, Dong
Wengier, Diego Leonardo
Shuai, Bin
Gui, Cai Ping
Muschietti, Jorge Prometeo
McCormick, Sheila
Tang, Wei Hua
author Zhang, Dong
author_facet Zhang, Dong
Wengier, Diego Leonardo
Shuai, Bin
Gui, Cai Ping
Muschietti, Jorge Prometeo
McCormick, Sheila
Tang, Wei Hua
author_role author
author2 Wengier, Diego Leonardo
Shuai, Bin
Gui, Cai Ping
Muschietti, Jorge Prometeo
McCormick, Sheila
Tang, Wei Hua
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Pollen
topic Pollen
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In flowering plants, the process of pollen germination and tube growth is required for successful fertilization. A pollen receptor kinase from tomato (Solanum lycopersicum), LePRK2, has been implicated in signaling during pollen germination and tube growth as well as in mediating pollen (tube)-pistil communication. Here we show that reduced expression of LePRK2 affects four aspects of pollen germination and tube growth. First, the percentage of pollen that germinates is reduced, and the time window for competence to germinate is also shorter. Second, the pollen tube growth rate is reduced both in vitro and in the pistil. Third, tip-localized superoxide production by pollen tubes cannot be increased by exogenous calcium ions. Fourth, pollen tubes have defects in responses to style extract component (STIL), an extracellular growth-promoting signal from the pistil. Pollen tubes transiently overexpressing LePRK2-fluorescent protein fusions had slightly wider tips, whereas pollen tubes coexpressing LePRK2 and its cytoplasmic partner protein KPP (a Rop-GEF) had much wider tips. Together these results show that LePRK2 positively regulates pollen germination and tube growth and is involved in transducing responses to extracellular growth-promoting signals.
Fil: Zhang, Dong. Chinese Academy of Sciences; República de China
Fil: Wengier, Diego Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; Argentina. University of California at Berkeley; Estados Unidos
Fil: Shuai, Bin. University of California at Berkeley; Estados Unidos
Fil: Gui, Cai Ping. Chinese Academy of Sciences; República de China
Fil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; Argentina
Fil: McCormick, Sheila. University of California at Berkeley; Estados Unidos
Fil: Tang, Wei Hua. Chinese Academy of Sciences; República de China. University of California at Berkeley; Estados Unidos
description In flowering plants, the process of pollen germination and tube growth is required for successful fertilization. A pollen receptor kinase from tomato (Solanum lycopersicum), LePRK2, has been implicated in signaling during pollen germination and tube growth as well as in mediating pollen (tube)-pistil communication. Here we show that reduced expression of LePRK2 affects four aspects of pollen germination and tube growth. First, the percentage of pollen that germinates is reduced, and the time window for competence to germinate is also shorter. Second, the pollen tube growth rate is reduced both in vitro and in the pistil. Third, tip-localized superoxide production by pollen tubes cannot be increased by exogenous calcium ions. Fourth, pollen tubes have defects in responses to style extract component (STIL), an extracellular growth-promoting signal from the pistil. Pollen tubes transiently overexpressing LePRK2-fluorescent protein fusions had slightly wider tips, whereas pollen tubes coexpressing LePRK2 and its cytoplasmic partner protein KPP (a Rop-GEF) had much wider tips. Together these results show that LePRK2 positively regulates pollen germination and tube growth and is involved in transducing responses to extracellular growth-promoting signals.
publishDate 2008
dc.date.none.fl_str_mv 2008-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79639
Zhang, Dong; Wengier, Diego Leonardo; Shuai, Bin; Gui, Cai Ping; Muschietti, Jorge Prometeo; et al.; The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth; American Society of Plant Biologist; Plant Physiology; 148; 3; 11-2008; 1368-1379
0032-0889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79639
identifier_str_mv Zhang, Dong; Wengier, Diego Leonardo; Shuai, Bin; Gui, Cai Ping; Muschietti, Jorge Prometeo; et al.; The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth; American Society of Plant Biologist; Plant Physiology; 148; 3; 11-2008; 1368-1379
0032-0889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/148/3/1368
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2577252/
info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.108.124420
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269816125128704
score 13.13397

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