Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth

Autores
Kaothien, Pulla; Sung, Han Ok; Shuai, Bin; Wengier, Diego Leonardo; Cotter, Robyn; Kelley, Dior; Kiriakopolos, Stephanie; Muschietti, Jorge Prometeo; McCormick, Sheila
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The pollen-specific receptor kinases LePRK1 and LePRK2 have localization and expression profiles that strongly suggest they play roles in pollen germination and tube growth. To identify downstream components of LePRK signaling, we used their cytoplasmic domains (CDs) as baits in yeast two-hybrid screens of a tomato pollen cDNA library. A pollen-specific protein we named kinase partner protein (KPP) interacted with the CDs of both LePRK1 and LePRK2 in yeast and in an in vitro pull-down assay, and with LePRK2 in a co-immunoprecipitation assay. KPP is a peripheral membrane protein and is phosphorylated in pollen. Pollen tubes over-expressing KPP developed balloon-like tips with abnormal cytoplasmic streaming and F-actin arrangements and plants over-expressing KPP exhibited impaired transmission of the transgene through the male. KPP-like genes are found only in plants; the 14 family members in Arabidopsis thaliana exhibit diverse expression patterns and potentially play roles in signaling pathways in other tissues.
Fil: Kaothien, Pulla. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados Unidos
Fil: Sung, Han Ok. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados Unidos
Fil: Shuai, Bin. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados Unidos
Fil: Wengier, Diego Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados Unidos
Fil: Cotter, Robyn. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados Unidos
Fil: Kelley, Dior. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados Unidos
Fil: Kiriakopolos, Stephanie. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados Unidos
Fil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: McCormick, Sheila. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados Unidos
Materia
Actin
Cytoplasmic Streaming
Signaling
Tomato
Vacuole
Yeast Two-Hybrid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79875
Acceder
id CONICETDig_e0bed29faf252c9682409904ad2fe71c
oai_identifier_str oai:ri.conicet.gov.ar:11336/79875
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growthKaothien, PullaSung, Han OkShuai, BinWengier, Diego LeonardoCotter, RobynKelley, DiorKiriakopolos, StephanieMuschietti, Jorge PrometeoMcCormick, SheilaActinCytoplasmic StreamingSignalingTomatoVacuoleYeast Two-Hybridhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The pollen-specific receptor kinases LePRK1 and LePRK2 have localization and expression profiles that strongly suggest they play roles in pollen germination and tube growth. To identify downstream components of LePRK signaling, we used their cytoplasmic domains (CDs) as baits in yeast two-hybrid screens of a tomato pollen cDNA library. A pollen-specific protein we named kinase partner protein (KPP) interacted with the CDs of both LePRK1 and LePRK2 in yeast and in an in vitro pull-down assay, and with LePRK2 in a co-immunoprecipitation assay. KPP is a peripheral membrane protein and is phosphorylated in pollen. Pollen tubes over-expressing KPP developed balloon-like tips with abnormal cytoplasmic streaming and F-actin arrangements and plants over-expressing KPP exhibited impaired transmission of the transgene through the male. KPP-like genes are found only in plants; the 14 family members in Arabidopsis thaliana exhibit diverse expression patterns and potentially play roles in signaling pathways in other tissues.Fil: Kaothien, Pulla. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados UnidosFil: Sung, Han Ok. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados UnidosFil: Shuai, Bin. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados UnidosFil: Wengier, Diego Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados UnidosFil: Cotter, Robyn. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados UnidosFil: Kelley, Dior. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados UnidosFil: Kiriakopolos, Stephanie. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados UnidosFil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: McCormick, Sheila. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados UnidosWiley Blackwell Publishing, Inc2005-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79875Kaothien, Pulla; Sung, Han Ok; Shuai, Bin; Wengier, Diego Leonardo; Cotter, Robyn; et al.; Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth; Wiley Blackwell Publishing, Inc; Plant Journal; 42; 4; 5-2005; 492-5030960-7412CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-313X.2005.02388.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-313X.2005.02388.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:39Zoai:ri.conicet.gov.ar:11336/79875instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:39.367CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth
title Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth
spellingShingle Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth
Kaothien, Pulla
Actin
Cytoplasmic Streaming
Signaling
Tomato
Vacuole
Yeast Two-Hybrid
title_short Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth
title_full Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth
title_fullStr Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth
title_full_unstemmed Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth
title_sort Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth
dc.creator.none.fl_str_mv Kaothien, Pulla
Sung, Han Ok
Shuai, Bin
Wengier, Diego Leonardo
Cotter, Robyn
Kelley, Dior
Kiriakopolos, Stephanie
Muschietti, Jorge Prometeo
McCormick, Sheila
author Kaothien, Pulla
author_facet Kaothien, Pulla
Sung, Han Ok
Shuai, Bin
Wengier, Diego Leonardo
Cotter, Robyn
Kelley, Dior
Kiriakopolos, Stephanie
Muschietti, Jorge Prometeo
McCormick, Sheila
author_role author
author2 Sung, Han Ok
Shuai, Bin
Wengier, Diego Leonardo
Cotter, Robyn
Kelley, Dior
Kiriakopolos, Stephanie
Muschietti, Jorge Prometeo
McCormick, Sheila
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Actin
Cytoplasmic Streaming
Signaling
Tomato
Vacuole
Yeast Two-Hybrid
topic Actin
Cytoplasmic Streaming
Signaling
Tomato
Vacuole
Yeast Two-Hybrid
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The pollen-specific receptor kinases LePRK1 and LePRK2 have localization and expression profiles that strongly suggest they play roles in pollen germination and tube growth. To identify downstream components of LePRK signaling, we used their cytoplasmic domains (CDs) as baits in yeast two-hybrid screens of a tomato pollen cDNA library. A pollen-specific protein we named kinase partner protein (KPP) interacted with the CDs of both LePRK1 and LePRK2 in yeast and in an in vitro pull-down assay, and with LePRK2 in a co-immunoprecipitation assay. KPP is a peripheral membrane protein and is phosphorylated in pollen. Pollen tubes over-expressing KPP developed balloon-like tips with abnormal cytoplasmic streaming and F-actin arrangements and plants over-expressing KPP exhibited impaired transmission of the transgene through the male. KPP-like genes are found only in plants; the 14 family members in Arabidopsis thaliana exhibit diverse expression patterns and potentially play roles in signaling pathways in other tissues.
Fil: Kaothien, Pulla. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados Unidos
Fil: Sung, Han Ok. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados Unidos
Fil: Shuai, Bin. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados Unidos
Fil: Wengier, Diego Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados Unidos
Fil: Cotter, Robyn. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados Unidos
Fil: Kelley, Dior. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados Unidos
Fil: Kiriakopolos, Stephanie. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados Unidos
Fil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: McCormick, Sheila. United States Department of Agriculture. Agricultural Research Service; Argentina. University of California at Berkeley; Estados Unidos
description The pollen-specific receptor kinases LePRK1 and LePRK2 have localization and expression profiles that strongly suggest they play roles in pollen germination and tube growth. To identify downstream components of LePRK signaling, we used their cytoplasmic domains (CDs) as baits in yeast two-hybrid screens of a tomato pollen cDNA library. A pollen-specific protein we named kinase partner protein (KPP) interacted with the CDs of both LePRK1 and LePRK2 in yeast and in an in vitro pull-down assay, and with LePRK2 in a co-immunoprecipitation assay. KPP is a peripheral membrane protein and is phosphorylated in pollen. Pollen tubes over-expressing KPP developed balloon-like tips with abnormal cytoplasmic streaming and F-actin arrangements and plants over-expressing KPP exhibited impaired transmission of the transgene through the male. KPP-like genes are found only in plants; the 14 family members in Arabidopsis thaliana exhibit diverse expression patterns and potentially play roles in signaling pathways in other tissues.
publishDate 2005
dc.date.none.fl_str_mv 2005-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79875
Kaothien, Pulla; Sung, Han Ok; Shuai, Bin; Wengier, Diego Leonardo; Cotter, Robyn; et al.; Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth; Wiley Blackwell Publishing, Inc; Plant Journal; 42; 4; 5-2005; 492-503
0960-7412
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79875
identifier_str_mv Kaothien, Pulla; Sung, Han Ok; Shuai, Bin; Wengier, Diego Leonardo; Cotter, Robyn; et al.; Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth; Wiley Blackwell Publishing, Inc; Plant Journal; 42; 4; 5-2005; 492-503
0960-7412
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-313X.2005.02388.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-313X.2005.02388.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613073314250752
score 13.070432

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