An Alternative Approach to Quantify Partition Processes in Confined Environments: The Electrochemical Behavior of PRODAN in Unilamellar Vesicles

Autores
Moyano, Fernando; Molina, Patricia Gabriela; Silber, Juana J.; Sereno, Leonides Edmundo; Correa, Nestor Mariano
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The main objective of this work is the investigation of the electroactive molecular probe: 6 - propionyl - 2- dimethyl amino naphthalene (PRODAN) behavior in large unilamellar vesicles (LUV) formed with the phospholipid 1,2-di-oleoyl-sn-glycero-3-phosphatidylcholine (DOPC) by using cyclic voltammetry (CV) techniques. The CV studies in pure water confirm our previous spectroscopic results that PRODAN self-aggregates due to its low water solubility. Moreover, the electrochemical results also reveal that the PRODAN aggregate species is non electroactive in the electrochemical potential region studied. In DOPC LUV media, the redox behavior of PRODAN shows how the LUV bilayer interacts with PRODAN aggregated species to form PRODAN monomer species. Moreover, the PRODAN electrochemical response allow us to propose a model to explain the electrochemical experimental results and, in conjunction with our measurements, to calculate the PRODAN partition constant (Kp) value between the water and the LUV bilayer pseudophases which coincides with the one obtained through an independent technique. Moreover, our electrochemical model allows us to calculate the diffusion coefficient (D) for the DOPC LUV which coincides with the D value obtained through dynamic light scattering (DLS). Thus, our data clearly show that the electrochemical measurements could be a powerful alternative approach to investigate the behavior of non-ionic electroactive molecules embed in a confined environment such as the LUV bilayer. Moreover, we believe that this approach can be used to investigate the behavior of non-optical molecular drugs embedded in bilayer media.
Fil: Moyano, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Molina, Patricia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Silber, Juana J.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Sereno, Leonides Edmundo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Correa, Nestor Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Materia
ELECTROCHEMISTRY
VESICLES
DOPC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242482
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/242482
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An Alternative Approach to Quantify Partition Processes in Confined Environments: The Electrochemical Behavior of PRODAN in Unilamellar VesiclesMoyano, FernandoMolina, Patricia GabrielaSilber, Juana J.Sereno, Leonides EdmundoCorrea, Nestor MarianoELECTROCHEMISTRYVESICLESDOPChttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The main objective of this work is the investigation of the electroactive molecular probe: 6 - propionyl - 2- dimethyl amino naphthalene (PRODAN) behavior in large unilamellar vesicles (LUV) formed with the phospholipid 1,2-di-oleoyl-sn-glycero-3-phosphatidylcholine (DOPC) by using cyclic voltammetry (CV) techniques. The CV studies in pure water confirm our previous spectroscopic results that PRODAN self-aggregates due to its low water solubility. Moreover, the electrochemical results also reveal that the PRODAN aggregate species is non electroactive in the electrochemical potential region studied. In DOPC LUV media, the redox behavior of PRODAN shows how the LUV bilayer interacts with PRODAN aggregated species to form PRODAN monomer species. Moreover, the PRODAN electrochemical response allow us to propose a model to explain the electrochemical experimental results and, in conjunction with our measurements, to calculate the PRODAN partition constant (Kp) value between the water and the LUV bilayer pseudophases which coincides with the one obtained through an independent technique. Moreover, our electrochemical model allows us to calculate the diffusion coefficient (D) for the DOPC LUV which coincides with the D value obtained through dynamic light scattering (DLS). Thus, our data clearly show that the electrochemical measurements could be a powerful alternative approach to investigate the behavior of non-ionic electroactive molecules embed in a confined environment such as the LUV bilayer. Moreover, we believe that this approach can be used to investigate the behavior of non-optical molecular drugs embedded in bilayer media.Fil: Moyano, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Molina, Patricia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Silber, Juana J.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Sereno, Leonides Edmundo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Correa, Nestor Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaWiley VCH Verlag2010-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242482Moyano, Fernando; Molina, Patricia Gabriela; Silber, Juana J.; Sereno, Leonides Edmundo; Correa, Nestor Mariano; An Alternative Approach to Quantify Partition Processes in Confined Environments: The Electrochemical Behavior of PRODAN in Unilamellar Vesicles; Wiley VCH Verlag; Chemphyschem; 11; 1; 1-2010; 236-2441439-4235CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cphc.200900557info:eu-repo/semantics/altIdentifier/doi/10.1002/cphc.200900557info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:12:05Zoai:ri.conicet.gov.ar:11336/242482instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:12:06.027CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An Alternative Approach to Quantify Partition Processes in Confined Environments: The Electrochemical Behavior of PRODAN in Unilamellar Vesicles
title An Alternative Approach to Quantify Partition Processes in Confined Environments: The Electrochemical Behavior of PRODAN in Unilamellar Vesicles
spellingShingle An Alternative Approach to Quantify Partition Processes in Confined Environments: The Electrochemical Behavior of PRODAN in Unilamellar Vesicles
Moyano, Fernando
ELECTROCHEMISTRY
VESICLES
DOPC
title_short An Alternative Approach to Quantify Partition Processes in Confined Environments: The Electrochemical Behavior of PRODAN in Unilamellar Vesicles
title_full An Alternative Approach to Quantify Partition Processes in Confined Environments: The Electrochemical Behavior of PRODAN in Unilamellar Vesicles
title_fullStr An Alternative Approach to Quantify Partition Processes in Confined Environments: The Electrochemical Behavior of PRODAN in Unilamellar Vesicles
title_full_unstemmed An Alternative Approach to Quantify Partition Processes in Confined Environments: The Electrochemical Behavior of PRODAN in Unilamellar Vesicles
title_sort An Alternative Approach to Quantify Partition Processes in Confined Environments: The Electrochemical Behavior of PRODAN in Unilamellar Vesicles
dc.creator.none.fl_str_mv Moyano, Fernando
Molina, Patricia Gabriela
Silber, Juana J.
Sereno, Leonides Edmundo
Correa, Nestor Mariano
author Moyano, Fernando
author_facet Moyano, Fernando
Molina, Patricia Gabriela
Silber, Juana J.
Sereno, Leonides Edmundo
Correa, Nestor Mariano
author_role author
author2 Molina, Patricia Gabriela
Silber, Juana J.
Sereno, Leonides Edmundo
Correa, Nestor Mariano
author2_role author
author
author
author
dc.subject.none.fl_str_mv ELECTROCHEMISTRY
VESICLES
DOPC
topic ELECTROCHEMISTRY
VESICLES
DOPC
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The main objective of this work is the investigation of the electroactive molecular probe: 6 - propionyl - 2- dimethyl amino naphthalene (PRODAN) behavior in large unilamellar vesicles (LUV) formed with the phospholipid 1,2-di-oleoyl-sn-glycero-3-phosphatidylcholine (DOPC) by using cyclic voltammetry (CV) techniques. The CV studies in pure water confirm our previous spectroscopic results that PRODAN self-aggregates due to its low water solubility. Moreover, the electrochemical results also reveal that the PRODAN aggregate species is non electroactive in the electrochemical potential region studied. In DOPC LUV media, the redox behavior of PRODAN shows how the LUV bilayer interacts with PRODAN aggregated species to form PRODAN monomer species. Moreover, the PRODAN electrochemical response allow us to propose a model to explain the electrochemical experimental results and, in conjunction with our measurements, to calculate the PRODAN partition constant (Kp) value between the water and the LUV bilayer pseudophases which coincides with the one obtained through an independent technique. Moreover, our electrochemical model allows us to calculate the diffusion coefficient (D) for the DOPC LUV which coincides with the D value obtained through dynamic light scattering (DLS). Thus, our data clearly show that the electrochemical measurements could be a powerful alternative approach to investigate the behavior of non-ionic electroactive molecules embed in a confined environment such as the LUV bilayer. Moreover, we believe that this approach can be used to investigate the behavior of non-optical molecular drugs embedded in bilayer media.
Fil: Moyano, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Molina, Patricia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Silber, Juana J.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Sereno, Leonides Edmundo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Correa, Nestor Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
description The main objective of this work is the investigation of the electroactive molecular probe: 6 - propionyl - 2- dimethyl amino naphthalene (PRODAN) behavior in large unilamellar vesicles (LUV) formed with the phospholipid 1,2-di-oleoyl-sn-glycero-3-phosphatidylcholine (DOPC) by using cyclic voltammetry (CV) techniques. The CV studies in pure water confirm our previous spectroscopic results that PRODAN self-aggregates due to its low water solubility. Moreover, the electrochemical results also reveal that the PRODAN aggregate species is non electroactive in the electrochemical potential region studied. In DOPC LUV media, the redox behavior of PRODAN shows how the LUV bilayer interacts with PRODAN aggregated species to form PRODAN monomer species. Moreover, the PRODAN electrochemical response allow us to propose a model to explain the electrochemical experimental results and, in conjunction with our measurements, to calculate the PRODAN partition constant (Kp) value between the water and the LUV bilayer pseudophases which coincides with the one obtained through an independent technique. Moreover, our electrochemical model allows us to calculate the diffusion coefficient (D) for the DOPC LUV which coincides with the D value obtained through dynamic light scattering (DLS). Thus, our data clearly show that the electrochemical measurements could be a powerful alternative approach to investigate the behavior of non-ionic electroactive molecules embed in a confined environment such as the LUV bilayer. Moreover, we believe that this approach can be used to investigate the behavior of non-optical molecular drugs embedded in bilayer media.
publishDate 2010
dc.date.none.fl_str_mv 2010-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242482
Moyano, Fernando; Molina, Patricia Gabriela; Silber, Juana J.; Sereno, Leonides Edmundo; Correa, Nestor Mariano; An Alternative Approach to Quantify Partition Processes in Confined Environments: The Electrochemical Behavior of PRODAN in Unilamellar Vesicles; Wiley VCH Verlag; Chemphyschem; 11; 1; 1-2010; 236-244
1439-4235
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242482
identifier_str_mv Moyano, Fernando; Molina, Patricia Gabriela; Silber, Juana J.; Sereno, Leonides Edmundo; Correa, Nestor Mariano; An Alternative Approach to Quantify Partition Processes in Confined Environments: The Electrochemical Behavior of PRODAN in Unilamellar Vesicles; Wiley VCH Verlag; Chemphyschem; 11; 1; 1-2010; 236-244
1439-4235
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cphc.200900557
info:eu-repo/semantics/altIdentifier/doi/10.1002/cphc.200900557
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.176822

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