The co-chaperone HOP3 participates in jasmonic acid signaling by regulating CORONATINE-INSENSITIVE 1 activity

Autores
Muñoz, Alfonso; Estrella Santamaria, M.; Fernández Bautista, Nuria; Mangano, Silvina; Toribio, René; Martínez, Manuel; Berrocal-Lobo, Marta; Diaz, Isabel; Mar Castellano, M.
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
HOPs (HSP70–HSP90 organizing proteins) are a highly conserved family of HSP70 and HSP90 co-chaperones whose role in assisting the folding of various hormonal receptors has been extensively studied in mammals. In plants, HOPs are mainly associated with stress response, but their potential involvement in hormonal networks remains completely unexplored. In this article we describe that a member of the HOP family, HOP3, is involved in the jasmonic acid (JA) pathway and is linked to plant defense responses not only to pathogens, but also to a generalist herbivore. The JA pathway regulates responses to Botrytis cinerea infection and to Tetranychus urticae feeding; our data demonstrate that the Arabidopsis (Arabidopsis thaliana) hop3-1 mutant shows an increased susceptibility to both. The hop3-1 mutant exhibits reduced sensitivity to JA derivatives in root growth assays and downregulation of different JA-responsive genes in response to methyl jasmonate, further revealing the relevance of HOP3 in the JA pathway. Interestingly, yeast two-hybrid assays and in planta co-immunoprecipitation assays found that HOP3 interacts with COI1, suggesting that COI1 is a target of HOP3. Consistent with this observation, COI1 activity is reduced in the hop3-1 mutant. All these data strongly suggest that, specifically among HOPs, HOP3 plays a relevant role in the JA pathway by regulating COI1 activity in response to JA and, consequently, participating in defense signaling to biotic stresses.
Fil: Muñoz, Alfonso. Universidad de Córdoba; España. Universidad Politécnica de Madrid; España
Fil: Estrella Santamaria, M.. Universidad Politécnica de Madrid; España
Fil: Fernández Bautista, Nuria. Universidad Politécnica de Madrid; España
Fil: Mangano, Silvina. Universidad Politécnica de Madrid; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Toribio, René. Universidad Politécnica de Madrid; España
Fil: Martínez, Manuel. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas; España. Universidad Politécnica de Madrid; España
Fil: Berrocal-Lobo, Marta. Universidad Politécnica de Madrid; España
Fil: Diaz, Isabel. Universidad Politécnica de Madrid; España. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas; España
Fil: Mar Castellano, M.. Universidad Politécnica de Madrid; España
Materia
Co-chaperone HOP3
Jasmonic acid
ORONATINE-INSENSITIVE 1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/212732
Acceder
id CONICETDig_efe3a91960011b89a237aefbbe81ef91
oai_identifier_str oai:ri.conicet.gov.ar:11336/212732
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The co-chaperone HOP3 participates in jasmonic acid signaling by regulating CORONATINE-INSENSITIVE 1 activityMuñoz, AlfonsoEstrella Santamaria, M.Fernández Bautista, NuriaMangano, SilvinaToribio, RenéMartínez, ManuelBerrocal-Lobo, MartaDiaz, IsabelMar Castellano, M.Co-chaperone HOP3Jasmonic acidORONATINE-INSENSITIVE 1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1HOPs (HSP70–HSP90 organizing proteins) are a highly conserved family of HSP70 and HSP90 co-chaperones whose role in assisting the folding of various hormonal receptors has been extensively studied in mammals. In plants, HOPs are mainly associated with stress response, but their potential involvement in hormonal networks remains completely unexplored. In this article we describe that a member of the HOP family, HOP3, is involved in the jasmonic acid (JA) pathway and is linked to plant defense responses not only to pathogens, but also to a generalist herbivore. The JA pathway regulates responses to Botrytis cinerea infection and to Tetranychus urticae feeding; our data demonstrate that the Arabidopsis (Arabidopsis thaliana) hop3-1 mutant shows an increased susceptibility to both. The hop3-1 mutant exhibits reduced sensitivity to JA derivatives in root growth assays and downregulation of different JA-responsive genes in response to methyl jasmonate, further revealing the relevance of HOP3 in the JA pathway. Interestingly, yeast two-hybrid assays and in planta co-immunoprecipitation assays found that HOP3 interacts with COI1, suggesting that COI1 is a target of HOP3. Consistent with this observation, COI1 activity is reduced in the hop3-1 mutant. All these data strongly suggest that, specifically among HOPs, HOP3 plays a relevant role in the JA pathway by regulating COI1 activity in response to JA and, consequently, participating in defense signaling to biotic stresses.Fil: Muñoz, Alfonso. Universidad de Córdoba; España. Universidad Politécnica de Madrid; EspañaFil: Estrella Santamaria, M.. Universidad Politécnica de Madrid; EspañaFil: Fernández Bautista, Nuria. Universidad Politécnica de Madrid; EspañaFil: Mangano, Silvina. Universidad Politécnica de Madrid; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Toribio, René. Universidad Politécnica de Madrid; EspañaFil: Martínez, Manuel. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas; España. Universidad Politécnica de Madrid; EspañaFil: Berrocal-Lobo, Marta. Universidad Politécnica de Madrid; EspañaFil: Diaz, Isabel. Universidad Politécnica de Madrid; España. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas; EspañaFil: Mar Castellano, M.. Universidad Politécnica de Madrid; EspañaAmerican Society of Plant Biologist2021-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/212732Muñoz, Alfonso; Estrella Santamaria, M.; Fernández Bautista, Nuria; Mangano, Silvina; Toribio, René; et al.; The co-chaperone HOP3 participates in jasmonic acid signaling by regulating CORONATINE-INSENSITIVE 1 activity; American Society of Plant Biologist; Plant Physiology; 187; 3; 11-2021; 1679-16890032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/plphys/kiab334info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/article/187/3/1679/6325517info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:59:09Zoai:ri.conicet.gov.ar:11336/212732instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:59:09.515CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The co-chaperone HOP3 participates in jasmonic acid signaling by regulating CORONATINE-INSENSITIVE 1 activity
title The co-chaperone HOP3 participates in jasmonic acid signaling by regulating CORONATINE-INSENSITIVE 1 activity
spellingShingle The co-chaperone HOP3 participates in jasmonic acid signaling by regulating CORONATINE-INSENSITIVE 1 activity
Muñoz, Alfonso
Co-chaperone HOP3
Jasmonic acid
ORONATINE-INSENSITIVE 1
title_short The co-chaperone HOP3 participates in jasmonic acid signaling by regulating CORONATINE-INSENSITIVE 1 activity
title_full The co-chaperone HOP3 participates in jasmonic acid signaling by regulating CORONATINE-INSENSITIVE 1 activity
title_fullStr The co-chaperone HOP3 participates in jasmonic acid signaling by regulating CORONATINE-INSENSITIVE 1 activity
title_full_unstemmed The co-chaperone HOP3 participates in jasmonic acid signaling by regulating CORONATINE-INSENSITIVE 1 activity
title_sort The co-chaperone HOP3 participates in jasmonic acid signaling by regulating CORONATINE-INSENSITIVE 1 activity
dc.creator.none.fl_str_mv Muñoz, Alfonso
Estrella Santamaria, M.
Fernández Bautista, Nuria
Mangano, Silvina
Toribio, René
Martínez, Manuel
Berrocal-Lobo, Marta
Diaz, Isabel
Mar Castellano, M.
author Muñoz, Alfonso
author_facet Muñoz, Alfonso
Estrella Santamaria, M.
Fernández Bautista, Nuria
Mangano, Silvina
Toribio, René
Martínez, Manuel
Berrocal-Lobo, Marta
Diaz, Isabel
Mar Castellano, M.
author_role author
author2 Estrella Santamaria, M.
Fernández Bautista, Nuria
Mangano, Silvina
Toribio, René
Martínez, Manuel
Berrocal-Lobo, Marta
Diaz, Isabel
Mar Castellano, M.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Co-chaperone HOP3
Jasmonic acid
ORONATINE-INSENSITIVE 1
topic Co-chaperone HOP3
Jasmonic acid
ORONATINE-INSENSITIVE 1
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv HOPs (HSP70–HSP90 organizing proteins) are a highly conserved family of HSP70 and HSP90 co-chaperones whose role in assisting the folding of various hormonal receptors has been extensively studied in mammals. In plants, HOPs are mainly associated with stress response, but their potential involvement in hormonal networks remains completely unexplored. In this article we describe that a member of the HOP family, HOP3, is involved in the jasmonic acid (JA) pathway and is linked to plant defense responses not only to pathogens, but also to a generalist herbivore. The JA pathway regulates responses to Botrytis cinerea infection and to Tetranychus urticae feeding; our data demonstrate that the Arabidopsis (Arabidopsis thaliana) hop3-1 mutant shows an increased susceptibility to both. The hop3-1 mutant exhibits reduced sensitivity to JA derivatives in root growth assays and downregulation of different JA-responsive genes in response to methyl jasmonate, further revealing the relevance of HOP3 in the JA pathway. Interestingly, yeast two-hybrid assays and in planta co-immunoprecipitation assays found that HOP3 interacts with COI1, suggesting that COI1 is a target of HOP3. Consistent with this observation, COI1 activity is reduced in the hop3-1 mutant. All these data strongly suggest that, specifically among HOPs, HOP3 plays a relevant role in the JA pathway by regulating COI1 activity in response to JA and, consequently, participating in defense signaling to biotic stresses.
Fil: Muñoz, Alfonso. Universidad de Córdoba; España. Universidad Politécnica de Madrid; España
Fil: Estrella Santamaria, M.. Universidad Politécnica de Madrid; España
Fil: Fernández Bautista, Nuria. Universidad Politécnica de Madrid; España
Fil: Mangano, Silvina. Universidad Politécnica de Madrid; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Toribio, René. Universidad Politécnica de Madrid; España
Fil: Martínez, Manuel. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas; España. Universidad Politécnica de Madrid; España
Fil: Berrocal-Lobo, Marta. Universidad Politécnica de Madrid; España
Fil: Diaz, Isabel. Universidad Politécnica de Madrid; España. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas; España
Fil: Mar Castellano, M.. Universidad Politécnica de Madrid; España
description HOPs (HSP70–HSP90 organizing proteins) are a highly conserved family of HSP70 and HSP90 co-chaperones whose role in assisting the folding of various hormonal receptors has been extensively studied in mammals. In plants, HOPs are mainly associated with stress response, but their potential involvement in hormonal networks remains completely unexplored. In this article we describe that a member of the HOP family, HOP3, is involved in the jasmonic acid (JA) pathway and is linked to plant defense responses not only to pathogens, but also to a generalist herbivore. The JA pathway regulates responses to Botrytis cinerea infection and to Tetranychus urticae feeding; our data demonstrate that the Arabidopsis (Arabidopsis thaliana) hop3-1 mutant shows an increased susceptibility to both. The hop3-1 mutant exhibits reduced sensitivity to JA derivatives in root growth assays and downregulation of different JA-responsive genes in response to methyl jasmonate, further revealing the relevance of HOP3 in the JA pathway. Interestingly, yeast two-hybrid assays and in planta co-immunoprecipitation assays found that HOP3 interacts with COI1, suggesting that COI1 is a target of HOP3. Consistent with this observation, COI1 activity is reduced in the hop3-1 mutant. All these data strongly suggest that, specifically among HOPs, HOP3 plays a relevant role in the JA pathway by regulating COI1 activity in response to JA and, consequently, participating in defense signaling to biotic stresses.
publishDate 2021
dc.date.none.fl_str_mv 2021-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/212732
Muñoz, Alfonso; Estrella Santamaria, M.; Fernández Bautista, Nuria; Mangano, Silvina; Toribio, René; et al.; The co-chaperone HOP3 participates in jasmonic acid signaling by regulating CORONATINE-INSENSITIVE 1 activity; American Society of Plant Biologist; Plant Physiology; 187; 3; 11-2021; 1679-1689
0032-0889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/212732
identifier_str_mv Muñoz, Alfonso; Estrella Santamaria, M.; Fernández Bautista, Nuria; Mangano, Silvina; Toribio, René; et al.; The co-chaperone HOP3 participates in jasmonic acid signaling by regulating CORONATINE-INSENSITIVE 1 activity; American Society of Plant Biologist; Plant Physiology; 187; 3; 11-2021; 1679-1689
0032-0889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/plphys/kiab334
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/article/187/3/1679/6325517
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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