Bioremediation of a petroleum hydrocarbon-contaminated Antarctic soil: Optimization of a biostimulation strategy using response-surface methodology (RSM)

Autores
Martinez Alvarez, Lucas Manuel; Lo Balbo, A.; Mac Cormack, W.P.; Ruberto, Lucas Adolfo Mauro
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bioremediation is a biotechnological approach to clean up contaminated soils. Among bioremediation strategies, biostimulation is a simple method which involves the modification of the soil physicochemical conditions in order to enhance the biological degradation of contaminants. One of the most common ways to do this is by the addition of macronutrients, mainly Nitrogen (N) and Phosphorus (P). Optimization of the amounts of N and P for a soil biostimulation strategy represents a key step prior to its application to a full-scale process. In this work, the response-surface methodology (RSM) was applied to optimize a biostimulation process for a hydrocarbon-contaminated Antarctic soil, considering a Carbon:Nitrogen:Phosporus (C:N:P) ratio of 100:10:1 as a reference. A faced-centered central composite design was used to determine the levels of the variables that lead to the optimum response values. Flasks containing contaminated soil and receiving different N and P amounts were incubated at 15 °C for 80 days. Biological activity and hydrocarbon concentration were evaluated. Results predicted that for the soil used in this experiment, the addition of 0.183 g N/kg and 0.0179 g P/kg leads to the highest hydrocarbon removal efficiency. The resulting C:N:P ratio (100:17.6:1.73) was different from that taken as reference (100:10:1), highlighting the usefulness of such an optimization. The hydrocarbon concentration decreased from 1042 (± 73) mg kg− 1 to 470 (± 37) mg kg− 1 in the most efficient combination tested.
Fil: Martinez Alvarez, Lucas Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: Lo Balbo, A.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Mac Cormack, W.P.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: Ruberto, Lucas Adolfo Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Materia
ANTARCTICA
BIOSTIMULATION
HYDROCARBON-CONTAMINATED SOIL
RESPONSE-SURFACE METHODOLOGY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94484
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/94484
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bioremediation of a petroleum hydrocarbon-contaminated Antarctic soil: Optimization of a biostimulation strategy using response-surface methodology (RSM)Martinez Alvarez, Lucas ManuelLo Balbo, A.Mac Cormack, W.P.Ruberto, Lucas Adolfo MauroANTARCTICABIOSTIMULATIONHYDROCARBON-CONTAMINATED SOILRESPONSE-SURFACE METHODOLOGYhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Bioremediation is a biotechnological approach to clean up contaminated soils. Among bioremediation strategies, biostimulation is a simple method which involves the modification of the soil physicochemical conditions in order to enhance the biological degradation of contaminants. One of the most common ways to do this is by the addition of macronutrients, mainly Nitrogen (N) and Phosphorus (P). Optimization of the amounts of N and P for a soil biostimulation strategy represents a key step prior to its application to a full-scale process. In this work, the response-surface methodology (RSM) was applied to optimize a biostimulation process for a hydrocarbon-contaminated Antarctic soil, considering a Carbon:Nitrogen:Phosporus (C:N:P) ratio of 100:10:1 as a reference. A faced-centered central composite design was used to determine the levels of the variables that lead to the optimum response values. Flasks containing contaminated soil and receiving different N and P amounts were incubated at 15 °C for 80 days. Biological activity and hydrocarbon concentration were evaluated. Results predicted that for the soil used in this experiment, the addition of 0.183 g N/kg and 0.0179 g P/kg leads to the highest hydrocarbon removal efficiency. The resulting C:N:P ratio (100:17.6:1.73) was different from that taken as reference (100:10:1), highlighting the usefulness of such an optimization. The hydrocarbon concentration decreased from 1042 (± 73) mg kg− 1 to 470 (± 37) mg kg− 1 in the most efficient combination tested.Fil: Martinez Alvarez, Lucas Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; ArgentinaFil: Lo Balbo, A.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Mac Cormack, W.P.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; ArgentinaFil: Ruberto, Lucas Adolfo Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; ArgentinaElsevier Science2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94484Martinez Alvarez, Lucas Manuel; Lo Balbo, A.; Mac Cormack, W.P.; Ruberto, Lucas Adolfo Mauro; Bioremediation of a petroleum hydrocarbon-contaminated Antarctic soil: Optimization of a biostimulation strategy using response-surface methodology (RSM); Elsevier Science; Cold Regions Science And Technology; 119; 11-2015; 61-670165-232XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0165232X15001640info:eu-repo/semantics/altIdentifier/doi/10.1016/j.coldregions.2015.07.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:00Zoai:ri.conicet.gov.ar:11336/94484instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:01.13CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bioremediation of a petroleum hydrocarbon-contaminated Antarctic soil: Optimization of a biostimulation strategy using response-surface methodology (RSM)
title Bioremediation of a petroleum hydrocarbon-contaminated Antarctic soil: Optimization of a biostimulation strategy using response-surface methodology (RSM)
spellingShingle Bioremediation of a petroleum hydrocarbon-contaminated Antarctic soil: Optimization of a biostimulation strategy using response-surface methodology (RSM)
Martinez Alvarez, Lucas Manuel
ANTARCTICA
BIOSTIMULATION
HYDROCARBON-CONTAMINATED SOIL
RESPONSE-SURFACE METHODOLOGY
title_short Bioremediation of a petroleum hydrocarbon-contaminated Antarctic soil: Optimization of a biostimulation strategy using response-surface methodology (RSM)
title_full Bioremediation of a petroleum hydrocarbon-contaminated Antarctic soil: Optimization of a biostimulation strategy using response-surface methodology (RSM)
title_fullStr Bioremediation of a petroleum hydrocarbon-contaminated Antarctic soil: Optimization of a biostimulation strategy using response-surface methodology (RSM)
title_full_unstemmed Bioremediation of a petroleum hydrocarbon-contaminated Antarctic soil: Optimization of a biostimulation strategy using response-surface methodology (RSM)
title_sort Bioremediation of a petroleum hydrocarbon-contaminated Antarctic soil: Optimization of a biostimulation strategy using response-surface methodology (RSM)
dc.creator.none.fl_str_mv Martinez Alvarez, Lucas Manuel
Lo Balbo, A.
Mac Cormack, W.P.
Ruberto, Lucas Adolfo Mauro
author Martinez Alvarez, Lucas Manuel
author_facet Martinez Alvarez, Lucas Manuel
Lo Balbo, A.
Mac Cormack, W.P.
Ruberto, Lucas Adolfo Mauro
author_role author
author2 Lo Balbo, A.
Mac Cormack, W.P.
Ruberto, Lucas Adolfo Mauro
author2_role author
author
author
dc.subject.none.fl_str_mv ANTARCTICA
BIOSTIMULATION
HYDROCARBON-CONTAMINATED SOIL
RESPONSE-SURFACE METHODOLOGY
topic ANTARCTICA
BIOSTIMULATION
HYDROCARBON-CONTAMINATED SOIL
RESPONSE-SURFACE METHODOLOGY
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Bioremediation is a biotechnological approach to clean up contaminated soils. Among bioremediation strategies, biostimulation is a simple method which involves the modification of the soil physicochemical conditions in order to enhance the biological degradation of contaminants. One of the most common ways to do this is by the addition of macronutrients, mainly Nitrogen (N) and Phosphorus (P). Optimization of the amounts of N and P for a soil biostimulation strategy represents a key step prior to its application to a full-scale process. In this work, the response-surface methodology (RSM) was applied to optimize a biostimulation process for a hydrocarbon-contaminated Antarctic soil, considering a Carbon:Nitrogen:Phosporus (C:N:P) ratio of 100:10:1 as a reference. A faced-centered central composite design was used to determine the levels of the variables that lead to the optimum response values. Flasks containing contaminated soil and receiving different N and P amounts were incubated at 15 °C for 80 days. Biological activity and hydrocarbon concentration were evaluated. Results predicted that for the soil used in this experiment, the addition of 0.183 g N/kg and 0.0179 g P/kg leads to the highest hydrocarbon removal efficiency. The resulting C:N:P ratio (100:17.6:1.73) was different from that taken as reference (100:10:1), highlighting the usefulness of such an optimization. The hydrocarbon concentration decreased from 1042 (± 73) mg kg− 1 to 470 (± 37) mg kg− 1 in the most efficient combination tested.
Fil: Martinez Alvarez, Lucas Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: Lo Balbo, A.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Mac Cormack, W.P.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: Ruberto, Lucas Adolfo Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
description Bioremediation is a biotechnological approach to clean up contaminated soils. Among bioremediation strategies, biostimulation is a simple method which involves the modification of the soil physicochemical conditions in order to enhance the biological degradation of contaminants. One of the most common ways to do this is by the addition of macronutrients, mainly Nitrogen (N) and Phosphorus (P). Optimization of the amounts of N and P for a soil biostimulation strategy represents a key step prior to its application to a full-scale process. In this work, the response-surface methodology (RSM) was applied to optimize a biostimulation process for a hydrocarbon-contaminated Antarctic soil, considering a Carbon:Nitrogen:Phosporus (C:N:P) ratio of 100:10:1 as a reference. A faced-centered central composite design was used to determine the levels of the variables that lead to the optimum response values. Flasks containing contaminated soil and receiving different N and P amounts were incubated at 15 °C for 80 days. Biological activity and hydrocarbon concentration were evaluated. Results predicted that for the soil used in this experiment, the addition of 0.183 g N/kg and 0.0179 g P/kg leads to the highest hydrocarbon removal efficiency. The resulting C:N:P ratio (100:17.6:1.73) was different from that taken as reference (100:10:1), highlighting the usefulness of such an optimization. The hydrocarbon concentration decreased from 1042 (± 73) mg kg− 1 to 470 (± 37) mg kg− 1 in the most efficient combination tested.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94484
Martinez Alvarez, Lucas Manuel; Lo Balbo, A.; Mac Cormack, W.P.; Ruberto, Lucas Adolfo Mauro; Bioremediation of a petroleum hydrocarbon-contaminated Antarctic soil: Optimization of a biostimulation strategy using response-surface methodology (RSM); Elsevier Science; Cold Regions Science And Technology; 119; 11-2015; 61-67
0165-232X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94484
identifier_str_mv Martinez Alvarez, Lucas Manuel; Lo Balbo, A.; Mac Cormack, W.P.; Ruberto, Lucas Adolfo Mauro; Bioremediation of a petroleum hydrocarbon-contaminated Antarctic soil: Optimization of a biostimulation strategy using response-surface methodology (RSM); Elsevier Science; Cold Regions Science And Technology; 119; 11-2015; 61-67
0165-232X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0165232X15001640
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.coldregions.2015.07.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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