Hydrocarbon removal and bacterial community structure in on-site biostimulated biopile systems designed for bioremediation of diesel-contaminated Antarctic soil
- Autores
- Dias, Romina Laura; Ruberto, Lucas Adolfo Mauro; Calabró López, Roberto Ariel; Lo Balbo, Alfredo; del Panno, Maria Teresa; Mac Cormack, Walter Patricio
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Several studies have shown that biostimulation can promote hydrocarbon bioremediation processes in Antarctic soils. However, the effect of the different nutrient sources on hydrocarbon removal heavily depends on the nutrients used and the soil characteristics. In this work, using a sample of chronically contaminated Antarctic soil that was exposed to a fresh hydrocarbon contamination, we analyzed how a complex organic nutrient source such as fish meal (FM) and a commercial fertilizer (OSEII) can affect hydrocarbon biodegradation and bacterial community composition. Both amended and unamended (control) biopiles were constructed and controlled at Carlini Station and sampled at days 0, 5, 16, 30 and 50 for microbiological, chemical and molecular analyses. FM caused a fast increase in both total heterotrophic and hydrocarbon degrading bacterial counts. These high values were maintained until the end of the assay, when statistically significant total hydrocarbon removal (71 %) was detected when compared with a control system. The FM biopile evidenced the dominance of members of the phylum Proteobacteria and a clear shift in bacterial structure at the final stage of the assay, when an increase of Actinobacteria was observed. The biopile containing the commercial fertilizer evidenced a hydrocarbon removal activity that was not statistically significant when compared with the untreated system and exhibited a bacterial community that differed from those observed in the unamended and FM-amended biopiles. In summary, biostimulation using FM in biopiles significantly enhanced the natural hydrocarbon-degradation activity of the Carlini station soils in biopile systems and caused significant changes in the bacterial community structure. The results will be considered for the future design of soil bioremediation protocols for Carlini Station and could also be taken into account to deal with diesel-contaminated soils from other cold-climate areas.
Fil: Dias, Romina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Ruberto, Lucas Adolfo Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: Calabró López, Roberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina
Fil: Lo Balbo, Alfredo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: del Panno, Maria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Mac Cormack, Walter Patricio. Universidad de Buenos Aires; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina - Materia
-
Antarctic Soils
Hydrocarbons
Biopiles
Biostimulation
Fish Meal
Commercial Fertilizer - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/37316
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Hydrocarbon removal and bacterial community structure in on-site biostimulated biopile systems designed for bioremediation of diesel-contaminated Antarctic soilDias, Romina LauraRuberto, Lucas Adolfo MauroCalabró López, Roberto ArielLo Balbo, Alfredodel Panno, Maria TeresaMac Cormack, Walter PatricioAntarctic SoilsHydrocarbonsBiopilesBiostimulationFish MealCommercial Fertilizerhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Several studies have shown that biostimulation can promote hydrocarbon bioremediation processes in Antarctic soils. However, the effect of the different nutrient sources on hydrocarbon removal heavily depends on the nutrients used and the soil characteristics. In this work, using a sample of chronically contaminated Antarctic soil that was exposed to a fresh hydrocarbon contamination, we analyzed how a complex organic nutrient source such as fish meal (FM) and a commercial fertilizer (OSEII) can affect hydrocarbon biodegradation and bacterial community composition. Both amended and unamended (control) biopiles were constructed and controlled at Carlini Station and sampled at days 0, 5, 16, 30 and 50 for microbiological, chemical and molecular analyses. FM caused a fast increase in both total heterotrophic and hydrocarbon degrading bacterial counts. These high values were maintained until the end of the assay, when statistically significant total hydrocarbon removal (71 %) was detected when compared with a control system. The FM biopile evidenced the dominance of members of the phylum Proteobacteria and a clear shift in bacterial structure at the final stage of the assay, when an increase of Actinobacteria was observed. The biopile containing the commercial fertilizer evidenced a hydrocarbon removal activity that was not statistically significant when compared with the untreated system and exhibited a bacterial community that differed from those observed in the unamended and FM-amended biopiles. In summary, biostimulation using FM in biopiles significantly enhanced the natural hydrocarbon-degradation activity of the Carlini station soils in biopile systems and caused significant changes in the bacterial community structure. The results will be considered for the future design of soil bioremediation protocols for Carlini Station and could also be taken into account to deal with diesel-contaminated soils from other cold-climate areas.Fil: Dias, Romina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Ruberto, Lucas Adolfo Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; ArgentinaFil: Calabró López, Roberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; ArgentinaFil: Lo Balbo, Alfredo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: del Panno, Maria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Mac Cormack, Walter Patricio. Universidad de Buenos Aires; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; ArgentinaSpringer2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37316Dias, Romina Laura; Ruberto, Lucas Adolfo Mauro; Calabró López, Roberto Ariel; Lo Balbo, Alfredo; del Panno, Maria Teresa; et al.; Hydrocarbon removal and bacterial community structure in on-site biostimulated biopile systems designed for bioremediation of diesel-contaminated Antarctic soil; Springer; Polar Biology; 38; 5; 12-2014; 677-6870722-4060CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00300-014-1630-7info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00300-014-1630-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:02:47Zoai:ri.conicet.gov.ar:11336/37316instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:02:47.509CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Hydrocarbon removal and bacterial community structure in on-site biostimulated biopile systems designed for bioremediation of diesel-contaminated Antarctic soil |
title |
Hydrocarbon removal and bacterial community structure in on-site biostimulated biopile systems designed for bioremediation of diesel-contaminated Antarctic soil |
spellingShingle |
Hydrocarbon removal and bacterial community structure in on-site biostimulated biopile systems designed for bioremediation of diesel-contaminated Antarctic soil Dias, Romina Laura Antarctic Soils Hydrocarbons Biopiles Biostimulation Fish Meal Commercial Fertilizer |
title_short |
Hydrocarbon removal and bacterial community structure in on-site biostimulated biopile systems designed for bioremediation of diesel-contaminated Antarctic soil |
title_full |
Hydrocarbon removal and bacterial community structure in on-site biostimulated biopile systems designed for bioremediation of diesel-contaminated Antarctic soil |
title_fullStr |
Hydrocarbon removal and bacterial community structure in on-site biostimulated biopile systems designed for bioremediation of diesel-contaminated Antarctic soil |
title_full_unstemmed |
Hydrocarbon removal and bacterial community structure in on-site biostimulated biopile systems designed for bioremediation of diesel-contaminated Antarctic soil |
title_sort |
Hydrocarbon removal and bacterial community structure in on-site biostimulated biopile systems designed for bioremediation of diesel-contaminated Antarctic soil |
dc.creator.none.fl_str_mv |
Dias, Romina Laura Ruberto, Lucas Adolfo Mauro Calabró López, Roberto Ariel Lo Balbo, Alfredo del Panno, Maria Teresa Mac Cormack, Walter Patricio |
author |
Dias, Romina Laura |
author_facet |
Dias, Romina Laura Ruberto, Lucas Adolfo Mauro Calabró López, Roberto Ariel Lo Balbo, Alfredo del Panno, Maria Teresa Mac Cormack, Walter Patricio |
author_role |
author |
author2 |
Ruberto, Lucas Adolfo Mauro Calabró López, Roberto Ariel Lo Balbo, Alfredo del Panno, Maria Teresa Mac Cormack, Walter Patricio |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Antarctic Soils Hydrocarbons Biopiles Biostimulation Fish Meal Commercial Fertilizer |
topic |
Antarctic Soils Hydrocarbons Biopiles Biostimulation Fish Meal Commercial Fertilizer |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Several studies have shown that biostimulation can promote hydrocarbon bioremediation processes in Antarctic soils. However, the effect of the different nutrient sources on hydrocarbon removal heavily depends on the nutrients used and the soil characteristics. In this work, using a sample of chronically contaminated Antarctic soil that was exposed to a fresh hydrocarbon contamination, we analyzed how a complex organic nutrient source such as fish meal (FM) and a commercial fertilizer (OSEII) can affect hydrocarbon biodegradation and bacterial community composition. Both amended and unamended (control) biopiles were constructed and controlled at Carlini Station and sampled at days 0, 5, 16, 30 and 50 for microbiological, chemical and molecular analyses. FM caused a fast increase in both total heterotrophic and hydrocarbon degrading bacterial counts. These high values were maintained until the end of the assay, when statistically significant total hydrocarbon removal (71 %) was detected when compared with a control system. The FM biopile evidenced the dominance of members of the phylum Proteobacteria and a clear shift in bacterial structure at the final stage of the assay, when an increase of Actinobacteria was observed. The biopile containing the commercial fertilizer evidenced a hydrocarbon removal activity that was not statistically significant when compared with the untreated system and exhibited a bacterial community that differed from those observed in the unamended and FM-amended biopiles. In summary, biostimulation using FM in biopiles significantly enhanced the natural hydrocarbon-degradation activity of the Carlini station soils in biopile systems and caused significant changes in the bacterial community structure. The results will be considered for the future design of soil bioremediation protocols for Carlini Station and could also be taken into account to deal with diesel-contaminated soils from other cold-climate areas. Fil: Dias, Romina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Ruberto, Lucas Adolfo Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina Fil: Calabró López, Roberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina Fil: Lo Balbo, Alfredo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: del Panno, Maria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Mac Cormack, Walter Patricio. Universidad de Buenos Aires; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina |
description |
Several studies have shown that biostimulation can promote hydrocarbon bioremediation processes in Antarctic soils. However, the effect of the different nutrient sources on hydrocarbon removal heavily depends on the nutrients used and the soil characteristics. In this work, using a sample of chronically contaminated Antarctic soil that was exposed to a fresh hydrocarbon contamination, we analyzed how a complex organic nutrient source such as fish meal (FM) and a commercial fertilizer (OSEII) can affect hydrocarbon biodegradation and bacterial community composition. Both amended and unamended (control) biopiles were constructed and controlled at Carlini Station and sampled at days 0, 5, 16, 30 and 50 for microbiological, chemical and molecular analyses. FM caused a fast increase in both total heterotrophic and hydrocarbon degrading bacterial counts. These high values were maintained until the end of the assay, when statistically significant total hydrocarbon removal (71 %) was detected when compared with a control system. The FM biopile evidenced the dominance of members of the phylum Proteobacteria and a clear shift in bacterial structure at the final stage of the assay, when an increase of Actinobacteria was observed. The biopile containing the commercial fertilizer evidenced a hydrocarbon removal activity that was not statistically significant when compared with the untreated system and exhibited a bacterial community that differed from those observed in the unamended and FM-amended biopiles. In summary, biostimulation using FM in biopiles significantly enhanced the natural hydrocarbon-degradation activity of the Carlini station soils in biopile systems and caused significant changes in the bacterial community structure. The results will be considered for the future design of soil bioremediation protocols for Carlini Station and could also be taken into account to deal with diesel-contaminated soils from other cold-climate areas. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/37316 Dias, Romina Laura; Ruberto, Lucas Adolfo Mauro; Calabró López, Roberto Ariel; Lo Balbo, Alfredo; del Panno, Maria Teresa; et al.; Hydrocarbon removal and bacterial community structure in on-site biostimulated biopile systems designed for bioremediation of diesel-contaminated Antarctic soil; Springer; Polar Biology; 38; 5; 12-2014; 677-687 0722-4060 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/37316 |
identifier_str_mv |
Dias, Romina Laura; Ruberto, Lucas Adolfo Mauro; Calabró López, Roberto Ariel; Lo Balbo, Alfredo; del Panno, Maria Teresa; et al.; Hydrocarbon removal and bacterial community structure in on-site biostimulated biopile systems designed for bioremediation of diesel-contaminated Antarctic soil; Springer; Polar Biology; 38; 5; 12-2014; 677-687 0722-4060 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00300-014-1630-7 info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00300-014-1630-7 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846083169616396288 |
score |
13.22299 |