The RALF1–FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair Growth

Autores
Zhu, Sirui; Estevez, Jose Manuel; Liao, Hongdong; Zhu, Yonghua; Yang, Tao; Li, Chiyu; Wang, Yichuan; Li, Lan; Liu, Xuanming; Martinez Pacheco, Javier; Guo, Hongwei; Yu, Feng
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The molecular links between extracellular signals and the regulation of localized protein synthesis in plant cells are poorly understood. Here, we show that in Arabidopsis thaliana, the extracellular peptide RALF1 and its receptor, the FERONIA receptor kinase, promote root hair (RH) tip growth by modulating protein synthesis. We found that RALF1 promotes FERONIA-mediated phosphorylation of eIF4E1, a eukaryotic translation initiation factor that plays a crucial role in the control of mRNA translation rate. Phosphorylated eIF4E1 increases mRNA affinity and modulates mRNA translation and, thus, protein synthesis. The mRNAs targeted by the RALF1–FERONIA–eIF4E1 module include ROP2 and RSL4, which are important regulators of RH cell polarity and growth. RALF1 and FERONIA are expressed in a polar manner in RHs, which facilitate eIF4E1 polar localization and thus may control local ROP2 translation. Moreover, we demonstrated that high-level accumulation of RSL4 exerts negative-feedback regulation of RALF1 expression by directly binding the RALF1 gene promoter, determining the final RH size. Our study reveals that the link between RALF1–FERONIA signaling and protein synthesis constitutes a novel component regulating cell expansion in these polar growing cells.
Fil: Zhu, Sirui. Hunan University; China
Fil: Estevez, Jose Manuel. Universidad Andrés Bello; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Liao, Hongdong. Hunan University; China
Fil: Zhu, Yonghua. Hunan University; China
Fil: Yang, Tao. Central South University of Forestry and Technology; China
Fil: Li, Chiyu. Hunan University; China
Fil: Wang, Yichuan. Southern University of Science and Technology; China
Fil: Li, Lan. Hunan University; China
Fil: Liu, Xuanming. Hunan University; China
Fil: Martinez Pacheco, Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Guo, Hongwei. Southern University of Science and Technology; China
Fil: Yu, Feng. Hunan University; China
Materia
EIF4E1
FER
POLAR CELL GROWTH
RALF1
ROOT HAIRS
RSL4
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/133618
Acceder
id CONICETDig_efc4c070f1b19a3a7a7e96d0caea206e
oai_identifier_str oai:ri.conicet.gov.ar:11336/133618
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The RALF1–FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair GrowthZhu, SiruiEstevez, Jose ManuelLiao, HongdongZhu, YonghuaYang, TaoLi, ChiyuWang, YichuanLi, LanLiu, XuanmingMartinez Pacheco, JavierGuo, HongweiYu, FengEIF4E1FERPOLAR CELL GROWTHRALF1ROOT HAIRSRSL4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The molecular links between extracellular signals and the regulation of localized protein synthesis in plant cells are poorly understood. Here, we show that in Arabidopsis thaliana, the extracellular peptide RALF1 and its receptor, the FERONIA receptor kinase, promote root hair (RH) tip growth by modulating protein synthesis. We found that RALF1 promotes FERONIA-mediated phosphorylation of eIF4E1, a eukaryotic translation initiation factor that plays a crucial role in the control of mRNA translation rate. Phosphorylated eIF4E1 increases mRNA affinity and modulates mRNA translation and, thus, protein synthesis. The mRNAs targeted by the RALF1–FERONIA–eIF4E1 module include ROP2 and RSL4, which are important regulators of RH cell polarity and growth. RALF1 and FERONIA are expressed in a polar manner in RHs, which facilitate eIF4E1 polar localization and thus may control local ROP2 translation. Moreover, we demonstrated that high-level accumulation of RSL4 exerts negative-feedback regulation of RALF1 expression by directly binding the RALF1 gene promoter, determining the final RH size. Our study reveals that the link between RALF1–FERONIA signaling and protein synthesis constitutes a novel component regulating cell expansion in these polar growing cells.Fil: Zhu, Sirui. Hunan University; ChinaFil: Estevez, Jose Manuel. Universidad Andrés Bello; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Liao, Hongdong. Hunan University; ChinaFil: Zhu, Yonghua. Hunan University; ChinaFil: Yang, Tao. Central South University of Forestry and Technology; ChinaFil: Li, Chiyu. Hunan University; ChinaFil: Wang, Yichuan. Southern University of Science and Technology; ChinaFil: Li, Lan. Hunan University; ChinaFil: Liu, Xuanming. Hunan University; ChinaFil: Martinez Pacheco, Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Guo, Hongwei. Southern University of Science and Technology; ChinaFil: Yu, Feng. Hunan University; ChinaOxford University Press2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/133618Zhu, Sirui; Estevez, Jose Manuel; Liao, Hongdong; Zhu, Yonghua; Yang, Tao; et al.; The RALF1–FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair Growth; Oxford University Press; Molecular Plant; 13; 5; 1-2020; 698-7161674-20521752-9867CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.molp.2019.12.014info:eu-repo/semantics/altIdentifier/url/https://www.cell.com/molecular-plant/fulltext/S1674-2052(19)30431-9info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1674205219304319info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:54Zoai:ri.conicet.gov.ar:11336/133618instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:54.366CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The RALF1–FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair Growth
title The RALF1–FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair Growth
spellingShingle The RALF1–FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair Growth
Zhu, Sirui
EIF4E1
FER
POLAR CELL GROWTH
RALF1
ROOT HAIRS
RSL4
title_short The RALF1–FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair Growth
title_full The RALF1–FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair Growth
title_fullStr The RALF1–FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair Growth
title_full_unstemmed The RALF1–FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair Growth
title_sort The RALF1–FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair Growth
dc.creator.none.fl_str_mv Zhu, Sirui
Estevez, Jose Manuel
Liao, Hongdong
Zhu, Yonghua
Yang, Tao
Li, Chiyu
Wang, Yichuan
Li, Lan
Liu, Xuanming
Martinez Pacheco, Javier
Guo, Hongwei
Yu, Feng
author Zhu, Sirui
author_facet Zhu, Sirui
Estevez, Jose Manuel
Liao, Hongdong
Zhu, Yonghua
Yang, Tao
Li, Chiyu
Wang, Yichuan
Li, Lan
Liu, Xuanming
Martinez Pacheco, Javier
Guo, Hongwei
Yu, Feng
author_role author
author2 Estevez, Jose Manuel
Liao, Hongdong
Zhu, Yonghua
Yang, Tao
Li, Chiyu
Wang, Yichuan
Li, Lan
Liu, Xuanming
Martinez Pacheco, Javier
Guo, Hongwei
Yu, Feng
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv EIF4E1
FER
POLAR CELL GROWTH
RALF1
ROOT HAIRS
RSL4
topic EIF4E1
FER
POLAR CELL GROWTH
RALF1
ROOT HAIRS
RSL4
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The molecular links between extracellular signals and the regulation of localized protein synthesis in plant cells are poorly understood. Here, we show that in Arabidopsis thaliana, the extracellular peptide RALF1 and its receptor, the FERONIA receptor kinase, promote root hair (RH) tip growth by modulating protein synthesis. We found that RALF1 promotes FERONIA-mediated phosphorylation of eIF4E1, a eukaryotic translation initiation factor that plays a crucial role in the control of mRNA translation rate. Phosphorylated eIF4E1 increases mRNA affinity and modulates mRNA translation and, thus, protein synthesis. The mRNAs targeted by the RALF1–FERONIA–eIF4E1 module include ROP2 and RSL4, which are important regulators of RH cell polarity and growth. RALF1 and FERONIA are expressed in a polar manner in RHs, which facilitate eIF4E1 polar localization and thus may control local ROP2 translation. Moreover, we demonstrated that high-level accumulation of RSL4 exerts negative-feedback regulation of RALF1 expression by directly binding the RALF1 gene promoter, determining the final RH size. Our study reveals that the link between RALF1–FERONIA signaling and protein synthesis constitutes a novel component regulating cell expansion in these polar growing cells.
Fil: Zhu, Sirui. Hunan University; China
Fil: Estevez, Jose Manuel. Universidad Andrés Bello; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Liao, Hongdong. Hunan University; China
Fil: Zhu, Yonghua. Hunan University; China
Fil: Yang, Tao. Central South University of Forestry and Technology; China
Fil: Li, Chiyu. Hunan University; China
Fil: Wang, Yichuan. Southern University of Science and Technology; China
Fil: Li, Lan. Hunan University; China
Fil: Liu, Xuanming. Hunan University; China
Fil: Martinez Pacheco, Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Guo, Hongwei. Southern University of Science and Technology; China
Fil: Yu, Feng. Hunan University; China
description The molecular links between extracellular signals and the regulation of localized protein synthesis in plant cells are poorly understood. Here, we show that in Arabidopsis thaliana, the extracellular peptide RALF1 and its receptor, the FERONIA receptor kinase, promote root hair (RH) tip growth by modulating protein synthesis. We found that RALF1 promotes FERONIA-mediated phosphorylation of eIF4E1, a eukaryotic translation initiation factor that plays a crucial role in the control of mRNA translation rate. Phosphorylated eIF4E1 increases mRNA affinity and modulates mRNA translation and, thus, protein synthesis. The mRNAs targeted by the RALF1–FERONIA–eIF4E1 module include ROP2 and RSL4, which are important regulators of RH cell polarity and growth. RALF1 and FERONIA are expressed in a polar manner in RHs, which facilitate eIF4E1 polar localization and thus may control local ROP2 translation. Moreover, we demonstrated that high-level accumulation of RSL4 exerts negative-feedback regulation of RALF1 expression by directly binding the RALF1 gene promoter, determining the final RH size. Our study reveals that the link between RALF1–FERONIA signaling and protein synthesis constitutes a novel component regulating cell expansion in these polar growing cells.
publishDate 2020
dc.date.none.fl_str_mv 2020-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/133618
Zhu, Sirui; Estevez, Jose Manuel; Liao, Hongdong; Zhu, Yonghua; Yang, Tao; et al.; The RALF1–FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair Growth; Oxford University Press; Molecular Plant; 13; 5; 1-2020; 698-716
1674-2052
1752-9867
CONICET Digital
CONICET
url http://hdl.handle.net/11336/133618
identifier_str_mv Zhu, Sirui; Estevez, Jose Manuel; Liao, Hongdong; Zhu, Yonghua; Yang, Tao; et al.; The RALF1–FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair Growth; Oxford University Press; Molecular Plant; 13; 5; 1-2020; 698-716
1674-2052
1752-9867
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.molp.2019.12.014
info:eu-repo/semantics/altIdentifier/url/https://www.cell.com/molecular-plant/fulltext/S1674-2052(19)30431-9
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1674205219304319
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269979463909376
score 13.13397

Publicaciones similares