Interplay between SERCA, 4E-BP, and eIF4E in the Drosophila heart

Autores
Santalla, Manuela; García, Alejandra; Mattiazzi, Ramona Alicia; Valverde, Carlos Alfredo; Schiemann, Ronja; Paululat, Achim; Hernández Ramírez, Greco; Meyer, Heiko; Ferrero, Paola Viviana
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Appropriate cardiac performance depends on a tightly controlled handling of Ca2+ in a broad range of species, from invertebrates to mammals. The role of the Ca2+ ATPase, SERCA, in Ca2+ handling is pivotal, and its activity is regulated, inter alia, by interacting with distinct proteins. Herein, we give evidence that 4E binding protein (4E-BP) is a novel regulator of SERCA activity in Drosophila melanogaster during cardiac function. Flies over-expressing 4E-BP showed improved cardiac performance in young individuals associated with incremented SERCA activity. Moreover, we demonstrate that SERCA interacts with translation initiation factors eIF4E-1, eIF4E-2 and eIF4E-4 in a yeast two-hybrid assay. The specific identification of eIF4E-4 in cardiac tissue leads us to propose that the interaction of elF4E-4 with SERCA may be the basis of the cardiac effects observed in 4E-BP over-expressing flies associated with incremented SERCA activity.
Fil: Santalla, Manuela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
Fil: García, Alejandra. Instituto Nacional de Cancerología; México
Fil: Mattiazzi, Ramona Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
Fil: Valverde, Carlos Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
Fil: Schiemann, Ronja. Universidad de Osnabrück; Alemania
Fil: Paululat, Achim. Universidad de Osnabrück; Alemania
Fil: Hernández Ramírez, Greco. Instituto Nacional de Cancerología; México
Fil: Meyer, Heiko. Universidad de Osnabrück; Alemania
Fil: Ferrero, Paola Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
Materia
4E-BP
Drosophila
heart
eIF4E
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/213132
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/213132
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Interplay between SERCA, 4E-BP, and eIF4E in the Drosophila heartSantalla, ManuelaGarcía, AlejandraMattiazzi, Ramona AliciaValverde, Carlos AlfredoSchiemann, RonjaPaululat, AchimHernández Ramírez, GrecoMeyer, HeikoFerrero, Paola Viviana4E-BPDrosophilahearteIF4Ehttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Appropriate cardiac performance depends on a tightly controlled handling of Ca2+ in a broad range of species, from invertebrates to mammals. The role of the Ca2+ ATPase, SERCA, in Ca2+ handling is pivotal, and its activity is regulated, inter alia, by interacting with distinct proteins. Herein, we give evidence that 4E binding protein (4E-BP) is a novel regulator of SERCA activity in Drosophila melanogaster during cardiac function. Flies over-expressing 4E-BP showed improved cardiac performance in young individuals associated with incremented SERCA activity. Moreover, we demonstrate that SERCA interacts with translation initiation factors eIF4E-1, eIF4E-2 and eIF4E-4 in a yeast two-hybrid assay. The specific identification of eIF4E-4 in cardiac tissue leads us to propose that the interaction of elF4E-4 with SERCA may be the basis of the cardiac effects observed in 4E-BP over-expressing flies associated with incremented SERCA activity.Fil: Santalla, Manuela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; ArgentinaFil: García, Alejandra. Instituto Nacional de Cancerología; MéxicoFil: Mattiazzi, Ramona Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; ArgentinaFil: Valverde, Carlos Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; ArgentinaFil: Schiemann, Ronja. Universidad de Osnabrück; AlemaniaFil: Paululat, Achim. Universidad de Osnabrück; AlemaniaFil: Hernández Ramírez, Greco. Instituto Nacional de Cancerología; MéxicoFil: Meyer, Heiko. Universidad de Osnabrück; AlemaniaFil: Ferrero, Paola Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; ArgentinaPublic Library of Science2022-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/213132Santalla, Manuela; García, Alejandra; Mattiazzi, Ramona Alicia; Valverde, Carlos Alfredo; Schiemann, Ronja; et al.; Interplay between SERCA, 4E-BP, and eIF4E in the Drosophila heart; Public Library of Science; Plos One; 17; 5; 5-2022; 1-191932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0267156info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0267156info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:45Zoai:ri.conicet.gov.ar:11336/213132instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:46.011CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Interplay between SERCA, 4E-BP, and eIF4E in the Drosophila heart
title Interplay between SERCA, 4E-BP, and eIF4E in the Drosophila heart
spellingShingle Interplay between SERCA, 4E-BP, and eIF4E in the Drosophila heart
Santalla, Manuela
4E-BP
Drosophila
heart
eIF4E
title_short Interplay between SERCA, 4E-BP, and eIF4E in the Drosophila heart
title_full Interplay between SERCA, 4E-BP, and eIF4E in the Drosophila heart
title_fullStr Interplay between SERCA, 4E-BP, and eIF4E in the Drosophila heart
title_full_unstemmed Interplay between SERCA, 4E-BP, and eIF4E in the Drosophila heart
title_sort Interplay between SERCA, 4E-BP, and eIF4E in the Drosophila heart
dc.creator.none.fl_str_mv Santalla, Manuela
García, Alejandra
Mattiazzi, Ramona Alicia
Valverde, Carlos Alfredo
Schiemann, Ronja
Paululat, Achim
Hernández Ramírez, Greco
Meyer, Heiko
Ferrero, Paola Viviana
author Santalla, Manuela
author_facet Santalla, Manuela
García, Alejandra
Mattiazzi, Ramona Alicia
Valverde, Carlos Alfredo
Schiemann, Ronja
Paululat, Achim
Hernández Ramírez, Greco
Meyer, Heiko
Ferrero, Paola Viviana
author_role author
author2 García, Alejandra
Mattiazzi, Ramona Alicia
Valverde, Carlos Alfredo
Schiemann, Ronja
Paululat, Achim
Hernández Ramírez, Greco
Meyer, Heiko
Ferrero, Paola Viviana
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv 4E-BP
Drosophila
heart
eIF4E
topic 4E-BP
Drosophila
heart
eIF4E
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Appropriate cardiac performance depends on a tightly controlled handling of Ca2+ in a broad range of species, from invertebrates to mammals. The role of the Ca2+ ATPase, SERCA, in Ca2+ handling is pivotal, and its activity is regulated, inter alia, by interacting with distinct proteins. Herein, we give evidence that 4E binding protein (4E-BP) is a novel regulator of SERCA activity in Drosophila melanogaster during cardiac function. Flies over-expressing 4E-BP showed improved cardiac performance in young individuals associated with incremented SERCA activity. Moreover, we demonstrate that SERCA interacts with translation initiation factors eIF4E-1, eIF4E-2 and eIF4E-4 in a yeast two-hybrid assay. The specific identification of eIF4E-4 in cardiac tissue leads us to propose that the interaction of elF4E-4 with SERCA may be the basis of the cardiac effects observed in 4E-BP over-expressing flies associated with incremented SERCA activity.
Fil: Santalla, Manuela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
Fil: García, Alejandra. Instituto Nacional de Cancerología; México
Fil: Mattiazzi, Ramona Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
Fil: Valverde, Carlos Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
Fil: Schiemann, Ronja. Universidad de Osnabrück; Alemania
Fil: Paululat, Achim. Universidad de Osnabrück; Alemania
Fil: Hernández Ramírez, Greco. Instituto Nacional de Cancerología; México
Fil: Meyer, Heiko. Universidad de Osnabrück; Alemania
Fil: Ferrero, Paola Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
description Appropriate cardiac performance depends on a tightly controlled handling of Ca2+ in a broad range of species, from invertebrates to mammals. The role of the Ca2+ ATPase, SERCA, in Ca2+ handling is pivotal, and its activity is regulated, inter alia, by interacting with distinct proteins. Herein, we give evidence that 4E binding protein (4E-BP) is a novel regulator of SERCA activity in Drosophila melanogaster during cardiac function. Flies over-expressing 4E-BP showed improved cardiac performance in young individuals associated with incremented SERCA activity. Moreover, we demonstrate that SERCA interacts with translation initiation factors eIF4E-1, eIF4E-2 and eIF4E-4 in a yeast two-hybrid assay. The specific identification of eIF4E-4 in cardiac tissue leads us to propose that the interaction of elF4E-4 with SERCA may be the basis of the cardiac effects observed in 4E-BP over-expressing flies associated with incremented SERCA activity.
publishDate 2022
dc.date.none.fl_str_mv 2022-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/213132
Santalla, Manuela; García, Alejandra; Mattiazzi, Ramona Alicia; Valverde, Carlos Alfredo; Schiemann, Ronja; et al.; Interplay between SERCA, 4E-BP, and eIF4E in the Drosophila heart; Public Library of Science; Plos One; 17; 5; 5-2022; 1-19
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/213132
identifier_str_mv Santalla, Manuela; García, Alejandra; Mattiazzi, Ramona Alicia; Valverde, Carlos Alfredo; Schiemann, Ronja; et al.; Interplay between SERCA, 4E-BP, and eIF4E in the Drosophila heart; Public Library of Science; Plos One; 17; 5; 5-2022; 1-19
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0267156
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0267156
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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