A Y-linked anti-Müllerian hormone duplication takes over a critical role in sex determination

Autores
Hattori, Ricardo Shohei; Murai, Yu; Oura, Miho; Masuda, Shuji; Majhi, Sullip K.; Sakamoto, Takashi; Fernandino, Juan Ignacio; Somoza, Gustavo Manuel; Yokota, Masashi; Strüssmann, Carlos A.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Gonadal sex determination in vertebrates generally follows a sequence of genetically programmed events. In what is seemingly becoming a pattern, all confirmed or current candidate “master” sex-determining genes reported in this group, e.g., SRY in eutherian mammals, DMY/dmrt1bY in medaka, DM-W in the African clawed frog, andDMRT1 in chicken encode transcription factors. In contrast, here we show that a male-specific, duplicated copy of the anti-Müllerian hormone (amh) is implicated in testicular development of the teleost fish Patagonian pejerrey (Odontesthes hatcheri). The gene, termed amhy because it is found in a single metacentric/submetacentric chromosome of XY individuals, is expressed much earlierthan the autosomal amh (6 d after fertilization vs. 12 wk after fertilization) and is localized to presumptive Sertoli cells of XY males during testicular differentiation.Moreover, amhy knockdown in XY embryos resulted in the up-regulation of foxl2 and cyp19a1a mRNAs and the development of ovaries. These results are evidence of a functional amh duplication in vertebrates and suggest that amhy may be the master sex-determining gene in this species. If confirmed, this would be a unique instance of a hormone-related gene, a member of the TGF-β superfamily, in such a role.
Fil: Hattori, Ricardo Shohei. Tokyo University of Marine Science and Technology; Japón
Fil: Murai, Yu. Tokyo University of Marine Science and Technology; Japón
Fil: Oura, Miho. Tokyo University of Marine Science and Technology; Japón
Fil: Masuda, Shuji. Tokyo University of Marine Science and Technology; Japón
Fil: Majhi, Sullip K.. Tokyo University of Marine Science and Technology; Japón
Fil: Sakamoto, Takashi. Tokyo University of Marine Science and Technology; Japón
Fil: Fernandino, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Yokota, Masashi. Tokyo University of Marine Science and Technology; Japón
Fil: Strüssmann, Carlos A.. Tokyo University of Marine Science and Technology; Japón
Materia
Amh
Sex determination
Pejerrey
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/268995
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/268995
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A Y-linked anti-Müllerian hormone duplication takes over a critical role in sex determinationHattori, Ricardo ShoheiMurai, YuOura, MihoMasuda, ShujiMajhi, Sullip K.Sakamoto, TakashiFernandino, Juan IgnacioSomoza, Gustavo ManuelYokota, MasashiStrüssmann, Carlos A.AmhSex determinationPejerreyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Gonadal sex determination in vertebrates generally follows a sequence of genetically programmed events. In what is seemingly becoming a pattern, all confirmed or current candidate “master” sex-determining genes reported in this group, e.g., SRY in eutherian mammals, DMY/dmrt1bY in medaka, DM-W in the African clawed frog, andDMRT1 in chicken encode transcription factors. In contrast, here we show that a male-specific, duplicated copy of the anti-Müllerian hormone (amh) is implicated in testicular development of the teleost fish Patagonian pejerrey (Odontesthes hatcheri). The gene, termed amhy because it is found in a single metacentric/submetacentric chromosome of XY individuals, is expressed much earlierthan the autosomal amh (6 d after fertilization vs. 12 wk after fertilization) and is localized to presumptive Sertoli cells of XY males during testicular differentiation.Moreover, amhy knockdown in XY embryos resulted in the up-regulation of foxl2 and cyp19a1a mRNAs and the development of ovaries. These results are evidence of a functional amh duplication in vertebrates and suggest that amhy may be the master sex-determining gene in this species. If confirmed, this would be a unique instance of a hormone-related gene, a member of the TGF-β superfamily, in such a role.Fil: Hattori, Ricardo Shohei. Tokyo University of Marine Science and Technology; JapónFil: Murai, Yu. Tokyo University of Marine Science and Technology; JapónFil: Oura, Miho. Tokyo University of Marine Science and Technology; JapónFil: Masuda, Shuji. Tokyo University of Marine Science and Technology; JapónFil: Majhi, Sullip K.. Tokyo University of Marine Science and Technology; JapónFil: Sakamoto, Takashi. Tokyo University of Marine Science and Technology; JapónFil: Fernandino, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Yokota, Masashi. Tokyo University of Marine Science and Technology; JapónFil: Strüssmann, Carlos A.. Tokyo University of Marine Science and Technology; JapónNational Academy of Sciences2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/268995Hattori, Ricardo Shohei; Murai, Yu; Oura, Miho; Masuda, Shuji; Majhi, Sullip K.; et al.; A Y-linked anti-Müllerian hormone duplication takes over a critical role in sex determination; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 109; 8; 2-2012; 2955-29590027-8424CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.pnas.org/doi/full/10.1073/pnas.1018392109info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.1018392109info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:03:38Zoai:ri.conicet.gov.ar:11336/268995instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:03:39.05CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A Y-linked anti-Müllerian hormone duplication takes over a critical role in sex determination
title A Y-linked anti-Müllerian hormone duplication takes over a critical role in sex determination
spellingShingle A Y-linked anti-Müllerian hormone duplication takes over a critical role in sex determination
Hattori, Ricardo Shohei
Amh
Sex determination
Pejerrey
title_short A Y-linked anti-Müllerian hormone duplication takes over a critical role in sex determination
title_full A Y-linked anti-Müllerian hormone duplication takes over a critical role in sex determination
title_fullStr A Y-linked anti-Müllerian hormone duplication takes over a critical role in sex determination
title_full_unstemmed A Y-linked anti-Müllerian hormone duplication takes over a critical role in sex determination
title_sort A Y-linked anti-Müllerian hormone duplication takes over a critical role in sex determination
dc.creator.none.fl_str_mv Hattori, Ricardo Shohei
Murai, Yu
Oura, Miho
Masuda, Shuji
Majhi, Sullip K.
Sakamoto, Takashi
Fernandino, Juan Ignacio
Somoza, Gustavo Manuel
Yokota, Masashi
Strüssmann, Carlos A.
author Hattori, Ricardo Shohei
author_facet Hattori, Ricardo Shohei
Murai, Yu
Oura, Miho
Masuda, Shuji
Majhi, Sullip K.
Sakamoto, Takashi
Fernandino, Juan Ignacio
Somoza, Gustavo Manuel
Yokota, Masashi
Strüssmann, Carlos A.
author_role author
author2 Murai, Yu
Oura, Miho
Masuda, Shuji
Majhi, Sullip K.
Sakamoto, Takashi
Fernandino, Juan Ignacio
Somoza, Gustavo Manuel
Yokota, Masashi
Strüssmann, Carlos A.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Amh
Sex determination
Pejerrey
topic Amh
Sex determination
Pejerrey
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Gonadal sex determination in vertebrates generally follows a sequence of genetically programmed events. In what is seemingly becoming a pattern, all confirmed or current candidate “master” sex-determining genes reported in this group, e.g., SRY in eutherian mammals, DMY/dmrt1bY in medaka, DM-W in the African clawed frog, andDMRT1 in chicken encode transcription factors. In contrast, here we show that a male-specific, duplicated copy of the anti-Müllerian hormone (amh) is implicated in testicular development of the teleost fish Patagonian pejerrey (Odontesthes hatcheri). The gene, termed amhy because it is found in a single metacentric/submetacentric chromosome of XY individuals, is expressed much earlierthan the autosomal amh (6 d after fertilization vs. 12 wk after fertilization) and is localized to presumptive Sertoli cells of XY males during testicular differentiation.Moreover, amhy knockdown in XY embryos resulted in the up-regulation of foxl2 and cyp19a1a mRNAs and the development of ovaries. These results are evidence of a functional amh duplication in vertebrates and suggest that amhy may be the master sex-determining gene in this species. If confirmed, this would be a unique instance of a hormone-related gene, a member of the TGF-β superfamily, in such a role.
Fil: Hattori, Ricardo Shohei. Tokyo University of Marine Science and Technology; Japón
Fil: Murai, Yu. Tokyo University of Marine Science and Technology; Japón
Fil: Oura, Miho. Tokyo University of Marine Science and Technology; Japón
Fil: Masuda, Shuji. Tokyo University of Marine Science and Technology; Japón
Fil: Majhi, Sullip K.. Tokyo University of Marine Science and Technology; Japón
Fil: Sakamoto, Takashi. Tokyo University of Marine Science and Technology; Japón
Fil: Fernandino, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Yokota, Masashi. Tokyo University of Marine Science and Technology; Japón
Fil: Strüssmann, Carlos A.. Tokyo University of Marine Science and Technology; Japón
description Gonadal sex determination in vertebrates generally follows a sequence of genetically programmed events. In what is seemingly becoming a pattern, all confirmed or current candidate “master” sex-determining genes reported in this group, e.g., SRY in eutherian mammals, DMY/dmrt1bY in medaka, DM-W in the African clawed frog, andDMRT1 in chicken encode transcription factors. In contrast, here we show that a male-specific, duplicated copy of the anti-Müllerian hormone (amh) is implicated in testicular development of the teleost fish Patagonian pejerrey (Odontesthes hatcheri). The gene, termed amhy because it is found in a single metacentric/submetacentric chromosome of XY individuals, is expressed much earlierthan the autosomal amh (6 d after fertilization vs. 12 wk after fertilization) and is localized to presumptive Sertoli cells of XY males during testicular differentiation.Moreover, amhy knockdown in XY embryos resulted in the up-regulation of foxl2 and cyp19a1a mRNAs and the development of ovaries. These results are evidence of a functional amh duplication in vertebrates and suggest that amhy may be the master sex-determining gene in this species. If confirmed, this would be a unique instance of a hormone-related gene, a member of the TGF-β superfamily, in such a role.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/268995
Hattori, Ricardo Shohei; Murai, Yu; Oura, Miho; Masuda, Shuji; Majhi, Sullip K.; et al.; A Y-linked anti-Müllerian hormone duplication takes over a critical role in sex determination; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 109; 8; 2-2012; 2955-2959
0027-8424
CONICET Digital
CONICET
url http://hdl.handle.net/11336/268995
identifier_str_mv Hattori, Ricardo Shohei; Murai, Yu; Oura, Miho; Masuda, Shuji; Majhi, Sullip K.; et al.; A Y-linked anti-Müllerian hormone duplication takes over a critical role in sex determination; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 109; 8; 2-2012; 2955-2959
0027-8424
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.pnas.org/doi/full/10.1073/pnas.1018392109
info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.1018392109
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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