The duplicated Y-specific amhy gene is conserved and linked to maleness in silversides of the genus Odontesthes

Autores
Hattori, Ricardo Shohei; Somoza, Gustavo Manuel; Fernandino, Juan Ignacio; Colautti, Dario César; Miyoshi, Kaho; Gong, Zhuang; Yamamoto, Yoji; Strüssmann, Carlos A.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sex-determining genes have been successively isolated in several teleosts. In Odontesthes hatcheri and O. bonariensis, the amhy gene has been identified as a master sex-determining gene. However, whether this gene is conserved along related species is still unknown. In this study, the presence of amhy and its association with phenotypic sex was analyzed in 10 species of Odontesthes genus. The primer sets from O. hatcheri that amplify both amhs successfully generated fragments that correspond to amha and amhy in all species. The full sequences of amhy and amha isolated for four key species revealed higher identity values among presumptive amhy, including the 0.5 Kbp insertion in the third intron and amhy-specific insertions/deletions. Amha was present in all specimens, regardless of species and sex, whereas amhy was amplified in most but not all phenotypic males. Complete association between amhy-homologue with maleness was found in O. argentinensis, O. incisa, O. mauleanum, O. perugiae, O. piquava, O. regia, and O. smitti, whereas O. humensis, O. mirinensis, and O. nigricans showed varied degrees of phenotypic/genotypic sex mismatch. The conservation of amhy gene in Odontesthes provide an interesting framework to study the evolution and the ecological interactions of genotypic and environmental sex determination in this group.
Fil: Hattori, Ricardo Shohei. Sao Paulo Fisheries Institue. Unidade de Pesquisa e Desenvolvimento de Campos do Jordão; Brasil
Fil: Somoza, Gustavo Manuel. Universidad Nacional de San Martin. Instituto Tecnológico de Chascomús - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Tecnológico de Chascomús; Argentina
Fil: Fernandino, Juan Ignacio. Universidad Nacional de San Martin. Instituto Tecnológico de Chascomús - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Tecnológico de Chascomús; Argentina
Fil: Colautti, Dario César. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; Argentina
Fil: Miyoshi, Kaho. Tokyo University of Marine Science and Technology; Japón
Fil: Gong, Zhuang. Zhejiang Ocean University; China
Fil: Yamamoto, Yoji. Tokyo University of Marine Science and Technology; Japón
Fil: Strüssmann, Carlos A.. Tokyo University of Marine Science and Technology; Japón
Materia
GENOTYPIC SEX DETERMINATION (GSD)
PEJERREY
SEX DETERMINATION
TEMPERATURE-DEPENDENT SEX DETERMINATION (TSD)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/128635
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/128635
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The duplicated Y-specific amhy gene is conserved and linked to maleness in silversides of the genus OdontesthesHattori, Ricardo ShoheiSomoza, Gustavo ManuelFernandino, Juan IgnacioColautti, Dario CésarMiyoshi, KahoGong, ZhuangYamamoto, YojiStrüssmann, Carlos A.GENOTYPIC SEX DETERMINATION (GSD)PEJERREYSEX DETERMINATIONTEMPERATURE-DEPENDENT SEX DETERMINATION (TSD)https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sex-determining genes have been successively isolated in several teleosts. In Odontesthes hatcheri and O. bonariensis, the amhy gene has been identified as a master sex-determining gene. However, whether this gene is conserved along related species is still unknown. In this study, the presence of amhy and its association with phenotypic sex was analyzed in 10 species of Odontesthes genus. The primer sets from O. hatcheri that amplify both amhs successfully generated fragments that correspond to amha and amhy in all species. The full sequences of amhy and amha isolated for four key species revealed higher identity values among presumptive amhy, including the 0.5 Kbp insertion in the third intron and amhy-specific insertions/deletions. Amha was present in all specimens, regardless of species and sex, whereas amhy was amplified in most but not all phenotypic males. Complete association between amhy-homologue with maleness was found in O. argentinensis, O. incisa, O. mauleanum, O. perugiae, O. piquava, O. regia, and O. smitti, whereas O. humensis, O. mirinensis, and O. nigricans showed varied degrees of phenotypic/genotypic sex mismatch. The conservation of amhy gene in Odontesthes provide an interesting framework to study the evolution and the ecological interactions of genotypic and environmental sex determination in this group.Fil: Hattori, Ricardo Shohei. Sao Paulo Fisheries Institue. Unidade de Pesquisa e Desenvolvimento de Campos do Jordão; BrasilFil: Somoza, Gustavo Manuel. Universidad Nacional de San Martin. Instituto Tecnológico de Chascomús - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Tecnológico de Chascomús; ArgentinaFil: Fernandino, Juan Ignacio. Universidad Nacional de San Martin. Instituto Tecnológico de Chascomús - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Tecnológico de Chascomús; ArgentinaFil: Colautti, Dario César. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaFil: Miyoshi, Kaho. Tokyo University of Marine Science and Technology; JapónFil: Gong, Zhuang. Zhejiang Ocean University; ChinaFil: Yamamoto, Yoji. Tokyo University of Marine Science and Technology; JapónFil: Strüssmann, Carlos A.. Tokyo University of Marine Science and Technology; JapónMultidisciplinary Digital Publishing Institute AG2019-09-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/128635Hattori, Ricardo Shohei; Somoza, Gustavo Manuel; Fernandino, Juan Ignacio; Colautti, Dario César; Miyoshi, Kaho; et al.; The duplicated Y-specific amhy gene is conserved and linked to maleness in silversides of the genus Odontesthes; Multidisciplinary Digital Publishing Institute AG; Genes; 10; 9; 5-9-2019; 1-102073-4425CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/genes10090679info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4425/10/9/679info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/31491991/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:09:54Zoai:ri.conicet.gov.ar:11336/128635instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:09:55.149CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The duplicated Y-specific amhy gene is conserved and linked to maleness in silversides of the genus Odontesthes
title The duplicated Y-specific amhy gene is conserved and linked to maleness in silversides of the genus Odontesthes
spellingShingle The duplicated Y-specific amhy gene is conserved and linked to maleness in silversides of the genus Odontesthes
Hattori, Ricardo Shohei
GENOTYPIC SEX DETERMINATION (GSD)
PEJERREY
SEX DETERMINATION
TEMPERATURE-DEPENDENT SEX DETERMINATION (TSD)
title_short The duplicated Y-specific amhy gene is conserved and linked to maleness in silversides of the genus Odontesthes
title_full The duplicated Y-specific amhy gene is conserved and linked to maleness in silversides of the genus Odontesthes
title_fullStr The duplicated Y-specific amhy gene is conserved and linked to maleness in silversides of the genus Odontesthes
title_full_unstemmed The duplicated Y-specific amhy gene is conserved and linked to maleness in silversides of the genus Odontesthes
title_sort The duplicated Y-specific amhy gene is conserved and linked to maleness in silversides of the genus Odontesthes
dc.creator.none.fl_str_mv Hattori, Ricardo Shohei
Somoza, Gustavo Manuel
Fernandino, Juan Ignacio
Colautti, Dario César
Miyoshi, Kaho
Gong, Zhuang
Yamamoto, Yoji
Strüssmann, Carlos A.
author Hattori, Ricardo Shohei
author_facet Hattori, Ricardo Shohei
Somoza, Gustavo Manuel
Fernandino, Juan Ignacio
Colautti, Dario César
Miyoshi, Kaho
Gong, Zhuang
Yamamoto, Yoji
Strüssmann, Carlos A.
author_role author
author2 Somoza, Gustavo Manuel
Fernandino, Juan Ignacio
Colautti, Dario César
Miyoshi, Kaho
Gong, Zhuang
Yamamoto, Yoji
Strüssmann, Carlos A.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GENOTYPIC SEX DETERMINATION (GSD)
PEJERREY
SEX DETERMINATION
TEMPERATURE-DEPENDENT SEX DETERMINATION (TSD)
topic GENOTYPIC SEX DETERMINATION (GSD)
PEJERREY
SEX DETERMINATION
TEMPERATURE-DEPENDENT SEX DETERMINATION (TSD)
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sex-determining genes have been successively isolated in several teleosts. In Odontesthes hatcheri and O. bonariensis, the amhy gene has been identified as a master sex-determining gene. However, whether this gene is conserved along related species is still unknown. In this study, the presence of amhy and its association with phenotypic sex was analyzed in 10 species of Odontesthes genus. The primer sets from O. hatcheri that amplify both amhs successfully generated fragments that correspond to amha and amhy in all species. The full sequences of amhy and amha isolated for four key species revealed higher identity values among presumptive amhy, including the 0.5 Kbp insertion in the third intron and amhy-specific insertions/deletions. Amha was present in all specimens, regardless of species and sex, whereas amhy was amplified in most but not all phenotypic males. Complete association between amhy-homologue with maleness was found in O. argentinensis, O. incisa, O. mauleanum, O. perugiae, O. piquava, O. regia, and O. smitti, whereas O. humensis, O. mirinensis, and O. nigricans showed varied degrees of phenotypic/genotypic sex mismatch. The conservation of amhy gene in Odontesthes provide an interesting framework to study the evolution and the ecological interactions of genotypic and environmental sex determination in this group.
Fil: Hattori, Ricardo Shohei. Sao Paulo Fisheries Institue. Unidade de Pesquisa e Desenvolvimento de Campos do Jordão; Brasil
Fil: Somoza, Gustavo Manuel. Universidad Nacional de San Martin. Instituto Tecnológico de Chascomús - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Tecnológico de Chascomús; Argentina
Fil: Fernandino, Juan Ignacio. Universidad Nacional de San Martin. Instituto Tecnológico de Chascomús - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Tecnológico de Chascomús; Argentina
Fil: Colautti, Dario César. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; Argentina
Fil: Miyoshi, Kaho. Tokyo University of Marine Science and Technology; Japón
Fil: Gong, Zhuang. Zhejiang Ocean University; China
Fil: Yamamoto, Yoji. Tokyo University of Marine Science and Technology; Japón
Fil: Strüssmann, Carlos A.. Tokyo University of Marine Science and Technology; Japón
description Sex-determining genes have been successively isolated in several teleosts. In Odontesthes hatcheri and O. bonariensis, the amhy gene has been identified as a master sex-determining gene. However, whether this gene is conserved along related species is still unknown. In this study, the presence of amhy and its association with phenotypic sex was analyzed in 10 species of Odontesthes genus. The primer sets from O. hatcheri that amplify both amhs successfully generated fragments that correspond to amha and amhy in all species. The full sequences of amhy and amha isolated for four key species revealed higher identity values among presumptive amhy, including the 0.5 Kbp insertion in the third intron and amhy-specific insertions/deletions. Amha was present in all specimens, regardless of species and sex, whereas amhy was amplified in most but not all phenotypic males. Complete association between amhy-homologue with maleness was found in O. argentinensis, O. incisa, O. mauleanum, O. perugiae, O. piquava, O. regia, and O. smitti, whereas O. humensis, O. mirinensis, and O. nigricans showed varied degrees of phenotypic/genotypic sex mismatch. The conservation of amhy gene in Odontesthes provide an interesting framework to study the evolution and the ecological interactions of genotypic and environmental sex determination in this group.
publishDate 2019
dc.date.none.fl_str_mv 2019-09-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/128635
Hattori, Ricardo Shohei; Somoza, Gustavo Manuel; Fernandino, Juan Ignacio; Colautti, Dario César; Miyoshi, Kaho; et al.; The duplicated Y-specific amhy gene is conserved and linked to maleness in silversides of the genus Odontesthes; Multidisciplinary Digital Publishing Institute AG; Genes; 10; 9; 5-9-2019; 1-10
2073-4425
CONICET Digital
CONICET
url http://hdl.handle.net/11336/128635
identifier_str_mv Hattori, Ricardo Shohei; Somoza, Gustavo Manuel; Fernandino, Juan Ignacio; Colautti, Dario César; Miyoshi, Kaho; et al.; The duplicated Y-specific amhy gene is conserved and linked to maleness in silversides of the genus Odontesthes; Multidisciplinary Digital Publishing Institute AG; Genes; 10; 9; 5-9-2019; 1-10
2073-4425
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/genes10090679
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4425/10/9/679
info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/31491991/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute AG
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.000565

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