Role of nitric oxide on the vasorelaxant effect of atrial natriuretic peptide on rabbit aorta basal tone

Autores
Romano, Monica Liliana; Coviello, Alfredo; Jerez, Susana Josefina; Peral, Maria de Los Angeles
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The role of nitric oxide (NO) on the vasorelaxant effect of atrial natriuretic peptide (ANP) on the basal tone of rabbit aortic rings conditioned to angiotensin II (Ang II) was studied. ANP aortic relaxation and nitrite release were measured in the presence and absence of endothelium and a NO-synthase inhibitor. Ang II at 10 8 M triggered a contractile response, conditioning the vessel to a vasorelaxant effect of ANP (10 8 M). This effect was significantly enhanced by endothelium removal, NG-nitro-L-arginine methyl ester (L-NAME, 10 4 M), and methylene blue (10 5 M). ANP decrease of basal tone in Ang-II-sensitized aortic rings was improved when a higher concentration of Ang II was used (10 6 M). Basal and Ang-II-stimulated nitrite release were measured in stretched (S) and nonstretched (NS) aortic rings. Nitrite release was significantly increased in S rings (p < 0.001). L-NAME (10 4 M) partially inhibited nitrite release in both basal and Ang-II-stimulated S aortic rings. In NS aortic rings, the NO inhibitor did not inhibit basal nitrite release but blunted the Ang-II-stimulated nitrite level. A significant negative correlation between nitrite release and the ANP vasorelaxant effect on basal tone was dependent on the Ang-II-sensitizing dose. The present results demonstrate that ANP relaxant effects on aortic basal tone are related to NO levels, which are regulated by S- and Ang-II-concentration-dependent NO generation and quenching.Key words: atrial natriuretic peptide, nitric oxide, vascular reactivity, basal tone, rabbit aorta.
On a examiné le rôle joué par le monoxyde d’azote (NO) dans l’effet vasorelaxant du peptide natriurétique auriculaire (ANP) sur le tonus basal d’anneaux aortiques de lapins sensibilisés à l’angiotensine II (Ang II). La relaxation aortique par l’ANP et la libération de nitrites ont été mesurées en présence et en absence d’endothélium et d’un inhibiteur de la NO synthase. L’Ang II (10–8 M) a déclenché une réponse contractile conditionnant le vaisseau à un effet vasorelaxant de l’ANP (10–8 M). Cet effet a été augmenté de manière significative par le retrait de l’endothélium ainsi que par NG-nitro-L-arginine méthyl ester (L-NAME, 10–4 M) et le bleu de méthylène (10–5 M). La diminution du tonus basal par l’ANP dans les anneaux aortiques sensibilisés à l’Ang II a été accrue lorsqu’une plus forte concentration d’Ang II a été utilisée (10–6 M). La libération de nitrites stimulée par l’Ang II et basale a été mesurée dans des anneaux aortiques étirés (É) et non étirés (NÉ). La libération de nitrites a été augmentée significativement dans les anneaux É (p < 0,001). L-NAME (10–4 M) a inhibé partiellement la libération basale ainsi que stimulée par l’Ang II dans ces anneaux. Dans les anneaux NÉ, l’inhibiteur de NO n’a pas inhibé la libération basale, mais il a diminué le taux de nitrites stimulés par l’Ang II. Une corrélation négative significative entre la libération de nitrites et l’effet vasorelaxant de l’ANP sur le tonus basal a été fonction de la dose sensibilisant à l’Ang II. Les résultats démontrent que l’effet relaxant de l’ANP sur le tonus basal aortique est lié aux taux de NO qui sont régulés par les muscles É ainsi qu’à la production et à l’inhibition de NO fonction de la concentration d’Ang II.
Fil: Romano, Monica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Coviello, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Jerez, Susana Josefina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Instituto Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Peral, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
Materia
ATRIAL NATRIURETIC PEPTIDE
NITRIC OXIDE
VASCULAR REACTIVITY
BASAL TONE
RABBIT AORTA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/100899

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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of nitric oxide on the vasorelaxant effect of atrial natriuretic peptide on rabbit aorta basal toneRomano, Monica LilianaCoviello, AlfredoJerez, Susana JosefinaPeral, Maria de Los AngelesATRIAL NATRIURETIC PEPTIDENITRIC OXIDEVASCULAR REACTIVITYBASAL TONERABBIT AORTAhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3The role of nitric oxide (NO) on the vasorelaxant effect of atrial natriuretic peptide (ANP) on the basal tone of rabbit aortic rings conditioned to angiotensin II (Ang II) was studied. ANP aortic relaxation and nitrite release were measured in the presence and absence of endothelium and a NO-synthase inhibitor. Ang II at 10 8 M triggered a contractile response, conditioning the vessel to a vasorelaxant effect of ANP (10 8 M). This effect was significantly enhanced by endothelium removal, NG-nitro-L-arginine methyl ester (L-NAME, 10 4 M), and methylene blue (10 5 M). ANP decrease of basal tone in Ang-II-sensitized aortic rings was improved when a higher concentration of Ang II was used (10 6 M). Basal and Ang-II-stimulated nitrite release were measured in stretched (S) and nonstretched (NS) aortic rings. Nitrite release was significantly increased in S rings (p < 0.001). L-NAME (10 4 M) partially inhibited nitrite release in both basal and Ang-II-stimulated S aortic rings. In NS aortic rings, the NO inhibitor did not inhibit basal nitrite release but blunted the Ang-II-stimulated nitrite level. A significant negative correlation between nitrite release and the ANP vasorelaxant effect on basal tone was dependent on the Ang-II-sensitizing dose. The present results demonstrate that ANP relaxant effects on aortic basal tone are related to NO levels, which are regulated by S- and Ang-II-concentration-dependent NO generation and quenching.Key words: atrial natriuretic peptide, nitric oxide, vascular reactivity, basal tone, rabbit aorta.On a examiné le rôle joué par le monoxyde d’azote (NO) dans l’effet vasorelaxant du peptide natriurétique auriculaire (ANP) sur le tonus basal d’anneaux aortiques de lapins sensibilisés à l’angiotensine II (Ang II). La relaxation aortique par l’ANP et la libération de nitrites ont été mesurées en présence et en absence d’endothélium et d’un inhibiteur de la NO synthase. L’Ang II (10–8 M) a déclenché une réponse contractile conditionnant le vaisseau à un effet vasorelaxant de l’ANP (10–8 M). Cet effet a été augmenté de manière significative par le retrait de l’endothélium ainsi que par NG-nitro-L-arginine méthyl ester (L-NAME, 10–4 M) et le bleu de méthylène (10–5 M). La diminution du tonus basal par l’ANP dans les anneaux aortiques sensibilisés à l’Ang II a été accrue lorsqu’une plus forte concentration d’Ang II a été utilisée (10–6 M). La libération de nitrites stimulée par l’Ang II et basale a été mesurée dans des anneaux aortiques étirés (É) et non étirés (NÉ). La libération de nitrites a été augmentée significativement dans les anneaux É (p < 0,001). L-NAME (10–4 M) a inhibé partiellement la libération basale ainsi que stimulée par l’Ang II dans ces anneaux. Dans les anneaux NÉ, l’inhibiteur de NO n’a pas inhibé la libération basale, mais il a diminué le taux de nitrites stimulés par l’Ang II. Une corrélation négative significative entre la libération de nitrites et l’effet vasorelaxant de l’ANP sur le tonus basal a été fonction de la dose sensibilisant à l’Ang II. Les résultats démontrent que l’effet relaxant de l’ANP sur le tonus basal aortique est lié aux taux de NO qui sont régulés par les muscles É ainsi qu’à la production et à l’inhibition de NO fonction de la concentration d’Ang II.Fil: Romano, Monica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Coviello, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Jerez, Susana Josefina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Instituto Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Peral, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; ArgentinaNational Research Council Canada-NRC Research Press2002-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/mswordapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/100899Romano, Monica Liliana; Coviello, Alfredo; Jerez, Susana Josefina; Peral, Maria de Los Angeles; Role of nitric oxide on the vasorelaxant effect of atrial natriuretic peptide on rabbit aorta basal tone; National Research Council Canada-NRC Research Press; Canadian Journal Of Physiology And Pharmacology; 80; 10; 10-2002; 1022-10290008-42121205-7541CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1139/y02-130info:eu-repo/semantics/altIdentifier/url/https://www.nrcresearchpress.com/doi/10.1139/y02-130#.XjB87mhKiUkinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:12Zoai:ri.conicet.gov.ar:11336/100899instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:12.792CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of nitric oxide on the vasorelaxant effect of atrial natriuretic peptide on rabbit aorta basal tone
title Role of nitric oxide on the vasorelaxant effect of atrial natriuretic peptide on rabbit aorta basal tone
spellingShingle Role of nitric oxide on the vasorelaxant effect of atrial natriuretic peptide on rabbit aorta basal tone
Romano, Monica Liliana
ATRIAL NATRIURETIC PEPTIDE
NITRIC OXIDE
VASCULAR REACTIVITY
BASAL TONE
RABBIT AORTA
title_short Role of nitric oxide on the vasorelaxant effect of atrial natriuretic peptide on rabbit aorta basal tone
title_full Role of nitric oxide on the vasorelaxant effect of atrial natriuretic peptide on rabbit aorta basal tone
title_fullStr Role of nitric oxide on the vasorelaxant effect of atrial natriuretic peptide on rabbit aorta basal tone
title_full_unstemmed Role of nitric oxide on the vasorelaxant effect of atrial natriuretic peptide on rabbit aorta basal tone
title_sort Role of nitric oxide on the vasorelaxant effect of atrial natriuretic peptide on rabbit aorta basal tone
dc.creator.none.fl_str_mv Romano, Monica Liliana
Coviello, Alfredo
Jerez, Susana Josefina
Peral, Maria de Los Angeles
author Romano, Monica Liliana
author_facet Romano, Monica Liliana
Coviello, Alfredo
Jerez, Susana Josefina
Peral, Maria de Los Angeles
author_role author
author2 Coviello, Alfredo
Jerez, Susana Josefina
Peral, Maria de Los Angeles
author2_role author
author
author
dc.subject.none.fl_str_mv ATRIAL NATRIURETIC PEPTIDE
NITRIC OXIDE
VASCULAR REACTIVITY
BASAL TONE
RABBIT AORTA
topic ATRIAL NATRIURETIC PEPTIDE
NITRIC OXIDE
VASCULAR REACTIVITY
BASAL TONE
RABBIT AORTA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The role of nitric oxide (NO) on the vasorelaxant effect of atrial natriuretic peptide (ANP) on the basal tone of rabbit aortic rings conditioned to angiotensin II (Ang II) was studied. ANP aortic relaxation and nitrite release were measured in the presence and absence of endothelium and a NO-synthase inhibitor. Ang II at 10 8 M triggered a contractile response, conditioning the vessel to a vasorelaxant effect of ANP (10 8 M). This effect was significantly enhanced by endothelium removal, NG-nitro-L-arginine methyl ester (L-NAME, 10 4 M), and methylene blue (10 5 M). ANP decrease of basal tone in Ang-II-sensitized aortic rings was improved when a higher concentration of Ang II was used (10 6 M). Basal and Ang-II-stimulated nitrite release were measured in stretched (S) and nonstretched (NS) aortic rings. Nitrite release was significantly increased in S rings (p < 0.001). L-NAME (10 4 M) partially inhibited nitrite release in both basal and Ang-II-stimulated S aortic rings. In NS aortic rings, the NO inhibitor did not inhibit basal nitrite release but blunted the Ang-II-stimulated nitrite level. A significant negative correlation between nitrite release and the ANP vasorelaxant effect on basal tone was dependent on the Ang-II-sensitizing dose. The present results demonstrate that ANP relaxant effects on aortic basal tone are related to NO levels, which are regulated by S- and Ang-II-concentration-dependent NO generation and quenching.Key words: atrial natriuretic peptide, nitric oxide, vascular reactivity, basal tone, rabbit aorta.
On a examiné le rôle joué par le monoxyde d’azote (NO) dans l’effet vasorelaxant du peptide natriurétique auriculaire (ANP) sur le tonus basal d’anneaux aortiques de lapins sensibilisés à l’angiotensine II (Ang II). La relaxation aortique par l’ANP et la libération de nitrites ont été mesurées en présence et en absence d’endothélium et d’un inhibiteur de la NO synthase. L’Ang II (10–8 M) a déclenché une réponse contractile conditionnant le vaisseau à un effet vasorelaxant de l’ANP (10–8 M). Cet effet a été augmenté de manière significative par le retrait de l’endothélium ainsi que par NG-nitro-L-arginine méthyl ester (L-NAME, 10–4 M) et le bleu de méthylène (10–5 M). La diminution du tonus basal par l’ANP dans les anneaux aortiques sensibilisés à l’Ang II a été accrue lorsqu’une plus forte concentration d’Ang II a été utilisée (10–6 M). La libération de nitrites stimulée par l’Ang II et basale a été mesurée dans des anneaux aortiques étirés (É) et non étirés (NÉ). La libération de nitrites a été augmentée significativement dans les anneaux É (p < 0,001). L-NAME (10–4 M) a inhibé partiellement la libération basale ainsi que stimulée par l’Ang II dans ces anneaux. Dans les anneaux NÉ, l’inhibiteur de NO n’a pas inhibé la libération basale, mais il a diminué le taux de nitrites stimulés par l’Ang II. Une corrélation négative significative entre la libération de nitrites et l’effet vasorelaxant de l’ANP sur le tonus basal a été fonction de la dose sensibilisant à l’Ang II. Les résultats démontrent que l’effet relaxant de l’ANP sur le tonus basal aortique est lié aux taux de NO qui sont régulés par les muscles É ainsi qu’à la production et à l’inhibition de NO fonction de la concentration d’Ang II.
Fil: Romano, Monica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Coviello, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Jerez, Susana Josefina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Instituto Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Peral, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
description The role of nitric oxide (NO) on the vasorelaxant effect of atrial natriuretic peptide (ANP) on the basal tone of rabbit aortic rings conditioned to angiotensin II (Ang II) was studied. ANP aortic relaxation and nitrite release were measured in the presence and absence of endothelium and a NO-synthase inhibitor. Ang II at 10 8 M triggered a contractile response, conditioning the vessel to a vasorelaxant effect of ANP (10 8 M). This effect was significantly enhanced by endothelium removal, NG-nitro-L-arginine methyl ester (L-NAME, 10 4 M), and methylene blue (10 5 M). ANP decrease of basal tone in Ang-II-sensitized aortic rings was improved when a higher concentration of Ang II was used (10 6 M). Basal and Ang-II-stimulated nitrite release were measured in stretched (S) and nonstretched (NS) aortic rings. Nitrite release was significantly increased in S rings (p < 0.001). L-NAME (10 4 M) partially inhibited nitrite release in both basal and Ang-II-stimulated S aortic rings. In NS aortic rings, the NO inhibitor did not inhibit basal nitrite release but blunted the Ang-II-stimulated nitrite level. A significant negative correlation between nitrite release and the ANP vasorelaxant effect on basal tone was dependent on the Ang-II-sensitizing dose. The present results demonstrate that ANP relaxant effects on aortic basal tone are related to NO levels, which are regulated by S- and Ang-II-concentration-dependent NO generation and quenching.Key words: atrial natriuretic peptide, nitric oxide, vascular reactivity, basal tone, rabbit aorta.
publishDate 2002
dc.date.none.fl_str_mv 2002-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/100899
Romano, Monica Liliana; Coviello, Alfredo; Jerez, Susana Josefina; Peral, Maria de Los Angeles; Role of nitric oxide on the vasorelaxant effect of atrial natriuretic peptide on rabbit aorta basal tone; National Research Council Canada-NRC Research Press; Canadian Journal Of Physiology And Pharmacology; 80; 10; 10-2002; 1022-1029
0008-4212
1205-7541
CONICET Digital
CONICET
url http://hdl.handle.net/11336/100899
identifier_str_mv Romano, Monica Liliana; Coviello, Alfredo; Jerez, Susana Josefina; Peral, Maria de Los Angeles; Role of nitric oxide on the vasorelaxant effect of atrial natriuretic peptide on rabbit aorta basal tone; National Research Council Canada-NRC Research Press; Canadian Journal Of Physiology And Pharmacology; 80; 10; 10-2002; 1022-1029
0008-4212
1205-7541
CONICET Digital
CONICET
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
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