Integrated Process of Biosurfactant Production by Bacillus atrophaeus ATCC-9372 Using an Air-Lift Bioreactor Coupled to a Foam Fraction Column

Autores
Rocha, Maria de Fátima F.; Sobral Júnior, Paulo S.; Leite, Milena S.; Pellegrini Malpiedi, Luciana; Pereira, Matheus M.; Soares, Cleide M. F.; Lima, Álvaro S.
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Biosurfactants are surface-active molecules, produced by several microorganisms, that possess unique properties such as low toxicity and biodegradability. Their application in various industries depends on their purity and their specific properties, such as emulsification and stability. Therefore, this study focuses on the production of biosurfactant from Bacillus atrophaeus in an air-liftbioreactor. It analyzes the effects of agitation rate and temperature on biosurfactant production, as well as the concurrent separation process using a foam fractionation column. Moreover, the ability of the produced biosurfactant to form emulsions in water with several substrates (vegetables oils, hydrocarbons, and fossil fuels) was determined, and the stability of the soybean oil?water emulsion (used as an example) at different temperatures and pH values was verified. The biosurfactant produced, tentatively identified as iturin, was only detected in the coalescent liquid after passing through the foam fractionation column, demonstrating the complete separation of the biosurfactant. The best operational conditions for production and separation were an air flow of 1.00 vvm and a temperature of 34 ◦C (emulsifier index (EI24) = 66.9%, and productivity (Pp) = 967.5% mL h−1 ). Vegetable oils, hydrocarbons, and fossil fuels were emulsified in water, highlighting the soybean oil,whose emulsion oil?water had the highest ES (3333.3 min) at a temperature of 50 ◦C and a pH value of 9.0.
Fil: Rocha, Maria de Fátima F.. Tiradentes University; Brasil
Fil: Sobral Júnior, Paulo S.. Tiradentes University; Brasil
Fil: Leite, Milena S.. Tiradentes University; Brasil
Fil: Pellegrini Malpiedi, Luciana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmaceuticas. Departamento de Química y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Pereira, Matheus M.. Universidade Tiradentes; Brasil
Fil: Soares, Cleide M. F.. Universidade Tiradentes; Brasil
Fil: Lima, Álvaro S.. Universidade Tiradentes; Brasil
Materia
FOAM FRACTIONATION COLUMN
INTGRATED PROCESS
STABILITY
BIOSURFACTANT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/255723
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Integrated Process of Biosurfactant Production by Bacillus atrophaeus ATCC-9372 Using an Air-Lift Bioreactor Coupled to a Foam Fraction ColumnRocha, Maria de Fátima F.Sobral Júnior, Paulo S.Leite, Milena S.Pellegrini Malpiedi, LucianaPereira, Matheus M.Soares, Cleide M. F.Lima, Álvaro S.FOAM FRACTIONATION COLUMNINTGRATED PROCESSSTABILITYBIOSURFACTANThttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Biosurfactants are surface-active molecules, produced by several microorganisms, that possess unique properties such as low toxicity and biodegradability. Their application in various industries depends on their purity and their specific properties, such as emulsification and stability. Therefore, this study focuses on the production of biosurfactant from Bacillus atrophaeus in an air-liftbioreactor. It analyzes the effects of agitation rate and temperature on biosurfactant production, as well as the concurrent separation process using a foam fractionation column. Moreover, the ability of the produced biosurfactant to form emulsions in water with several substrates (vegetables oils, hydrocarbons, and fossil fuels) was determined, and the stability of the soybean oil?water emulsion (used as an example) at different temperatures and pH values was verified. The biosurfactant produced, tentatively identified as iturin, was only detected in the coalescent liquid after passing through the foam fractionation column, demonstrating the complete separation of the biosurfactant. The best operational conditions for production and separation were an air flow of 1.00 vvm and a temperature of 34 ◦C (emulsifier index (EI24) = 66.9%, and productivity (Pp) = 967.5% mL h−1 ). Vegetable oils, hydrocarbons, and fossil fuels were emulsified in water, highlighting the soybean oil,whose emulsion oil?water had the highest ES (3333.3 min) at a temperature of 50 ◦C and a pH value of 9.0.Fil: Rocha, Maria de Fátima F.. Tiradentes University; BrasilFil: Sobral Júnior, Paulo S.. Tiradentes University; BrasilFil: Leite, Milena S.. Tiradentes University; BrasilFil: Pellegrini Malpiedi, Luciana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmaceuticas. Departamento de Química y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Pereira, Matheus M.. Universidade Tiradentes; BrasilFil: Soares, Cleide M. F.. Universidade Tiradentes; BrasilFil: Lima, Álvaro S.. Universidade Tiradentes; BrasilMDPI2023-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/255723Rocha, Maria de Fátima F.; Sobral Júnior, Paulo S. ; Leite, Milena S.; Pellegrini Malpiedi, Luciana; Pereira, Matheus M.; et al.; Integrated Process of Biosurfactant Production by Bacillus atrophaeus ATCC-9372 Using an Air-Lift Bioreactor Coupled to a Foam Fraction Column; MDPI; Fermentation; 9; 11; 11-2023; 1-132311-5637CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2311-5637/9/11/959info:eu-repo/semantics/altIdentifier/doi/10.3390/fermentation9110959info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:56:30Zoai:ri.conicet.gov.ar:11336/255723instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:56:30.938CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Integrated Process of Biosurfactant Production by Bacillus atrophaeus ATCC-9372 Using an Air-Lift Bioreactor Coupled to a Foam Fraction Column
title Integrated Process of Biosurfactant Production by Bacillus atrophaeus ATCC-9372 Using an Air-Lift Bioreactor Coupled to a Foam Fraction Column
spellingShingle Integrated Process of Biosurfactant Production by Bacillus atrophaeus ATCC-9372 Using an Air-Lift Bioreactor Coupled to a Foam Fraction Column
Rocha, Maria de Fátima F.
FOAM FRACTIONATION COLUMN
INTGRATED PROCESS
STABILITY
BIOSURFACTANT
title_short Integrated Process of Biosurfactant Production by Bacillus atrophaeus ATCC-9372 Using an Air-Lift Bioreactor Coupled to a Foam Fraction Column
title_full Integrated Process of Biosurfactant Production by Bacillus atrophaeus ATCC-9372 Using an Air-Lift Bioreactor Coupled to a Foam Fraction Column
title_fullStr Integrated Process of Biosurfactant Production by Bacillus atrophaeus ATCC-9372 Using an Air-Lift Bioreactor Coupled to a Foam Fraction Column
title_full_unstemmed Integrated Process of Biosurfactant Production by Bacillus atrophaeus ATCC-9372 Using an Air-Lift Bioreactor Coupled to a Foam Fraction Column
title_sort Integrated Process of Biosurfactant Production by Bacillus atrophaeus ATCC-9372 Using an Air-Lift Bioreactor Coupled to a Foam Fraction Column
dc.creator.none.fl_str_mv Rocha, Maria de Fátima F.
Sobral Júnior, Paulo S.
Leite, Milena S.
Pellegrini Malpiedi, Luciana
Pereira, Matheus M.
Soares, Cleide M. F.
Lima, Álvaro S.
author Rocha, Maria de Fátima F.
author_facet Rocha, Maria de Fátima F.
Sobral Júnior, Paulo S.
Leite, Milena S.
Pellegrini Malpiedi, Luciana
Pereira, Matheus M.
Soares, Cleide M. F.
Lima, Álvaro S.
author_role author
author2 Sobral Júnior, Paulo S.
Leite, Milena S.
Pellegrini Malpiedi, Luciana
Pereira, Matheus M.
Soares, Cleide M. F.
Lima, Álvaro S.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv FOAM FRACTIONATION COLUMN
INTGRATED PROCESS
STABILITY
BIOSURFACTANT
topic FOAM FRACTIONATION COLUMN
INTGRATED PROCESS
STABILITY
BIOSURFACTANT
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Biosurfactants are surface-active molecules, produced by several microorganisms, that possess unique properties such as low toxicity and biodegradability. Their application in various industries depends on their purity and their specific properties, such as emulsification and stability. Therefore, this study focuses on the production of biosurfactant from Bacillus atrophaeus in an air-liftbioreactor. It analyzes the effects of agitation rate and temperature on biosurfactant production, as well as the concurrent separation process using a foam fractionation column. Moreover, the ability of the produced biosurfactant to form emulsions in water with several substrates (vegetables oils, hydrocarbons, and fossil fuels) was determined, and the stability of the soybean oil?water emulsion (used as an example) at different temperatures and pH values was verified. The biosurfactant produced, tentatively identified as iturin, was only detected in the coalescent liquid after passing through the foam fractionation column, demonstrating the complete separation of the biosurfactant. The best operational conditions for production and separation were an air flow of 1.00 vvm and a temperature of 34 ◦C (emulsifier index (EI24) = 66.9%, and productivity (Pp) = 967.5% mL h−1 ). Vegetable oils, hydrocarbons, and fossil fuels were emulsified in water, highlighting the soybean oil,whose emulsion oil?water had the highest ES (3333.3 min) at a temperature of 50 ◦C and a pH value of 9.0.
Fil: Rocha, Maria de Fátima F.. Tiradentes University; Brasil
Fil: Sobral Júnior, Paulo S.. Tiradentes University; Brasil
Fil: Leite, Milena S.. Tiradentes University; Brasil
Fil: Pellegrini Malpiedi, Luciana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmaceuticas. Departamento de Química y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Pereira, Matheus M.. Universidade Tiradentes; Brasil
Fil: Soares, Cleide M. F.. Universidade Tiradentes; Brasil
Fil: Lima, Álvaro S.. Universidade Tiradentes; Brasil
description Biosurfactants are surface-active molecules, produced by several microorganisms, that possess unique properties such as low toxicity and biodegradability. Their application in various industries depends on their purity and their specific properties, such as emulsification and stability. Therefore, this study focuses on the production of biosurfactant from Bacillus atrophaeus in an air-liftbioreactor. It analyzes the effects of agitation rate and temperature on biosurfactant production, as well as the concurrent separation process using a foam fractionation column. Moreover, the ability of the produced biosurfactant to form emulsions in water with several substrates (vegetables oils, hydrocarbons, and fossil fuels) was determined, and the stability of the soybean oil?water emulsion (used as an example) at different temperatures and pH values was verified. The biosurfactant produced, tentatively identified as iturin, was only detected in the coalescent liquid after passing through the foam fractionation column, demonstrating the complete separation of the biosurfactant. The best operational conditions for production and separation were an air flow of 1.00 vvm and a temperature of 34 ◦C (emulsifier index (EI24) = 66.9%, and productivity (Pp) = 967.5% mL h−1 ). Vegetable oils, hydrocarbons, and fossil fuels were emulsified in water, highlighting the soybean oil,whose emulsion oil?water had the highest ES (3333.3 min) at a temperature of 50 ◦C and a pH value of 9.0.
publishDate 2023
dc.date.none.fl_str_mv 2023-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/255723
Rocha, Maria de Fátima F.; Sobral Júnior, Paulo S. ; Leite, Milena S.; Pellegrini Malpiedi, Luciana; Pereira, Matheus M.; et al.; Integrated Process of Biosurfactant Production by Bacillus atrophaeus ATCC-9372 Using an Air-Lift Bioreactor Coupled to a Foam Fraction Column; MDPI; Fermentation; 9; 11; 11-2023; 1-13
2311-5637
CONICET Digital
CONICET
url http://hdl.handle.net/11336/255723
identifier_str_mv Rocha, Maria de Fátima F.; Sobral Júnior, Paulo S. ; Leite, Milena S.; Pellegrini Malpiedi, Luciana; Pereira, Matheus M.; et al.; Integrated Process of Biosurfactant Production by Bacillus atrophaeus ATCC-9372 Using an Air-Lift Bioreactor Coupled to a Foam Fraction Column; MDPI; Fermentation; 9; 11; 11-2023; 1-13
2311-5637
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2311-5637/9/11/959
info:eu-repo/semantics/altIdentifier/doi/10.3390/fermentation9110959
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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