Extracts from strawberry by-products rich in phenolic compounds reduce the activity of apple polyphenol oxidase

Autores
Villamil Galindo, Johan Esteban; Van de Velde, Franco; Piagentini, Andrea
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The ultrasound-assisted extraction of phenolic compounds from strawberry by-products was studied varying the solvent type (water, ethanol 80%, methanol 80%, and acetone 80%), the formic acid concentration (0 and 0.5%), and the number of extraction steps (1 and 2). Total phenolic and total flavonoid compounds were determined spectrophotometrically and analyzed by PAD-HPLC. The antioxidant capacity (DPPH and FRAP assays) and the ability of extracts to inhibit apple (Malus domestica cv. ‘Red Delicious’) polyphenol oxidase (PPO) were also investigated. Extracts with acidified methanol in two-steps yielded the highest phenolic compound concentration (15.01 g/kg), and the highest antioxidant capacity. Agrimoniin was the major polyphenol found, and the extraction with acetone in two-steps produced the highest yield (2.45 g/kg). This ellagitannin was the only polyphenol that correlated (R2 > 0.80, p < 0.05) with the antioxidant capacity. Water and ethanol showed the lowest phenolic compound yields. However, extraction with these green solvents (water or ethanol) in two-steps showed polyphenol contents similar to those obtained with methanol or acetone in one-step (≈9 g/kg). Additionally, extracted polyphenols (0.24 g/L) produced 30% apple PPO inhibition, in a reversible ‘uncompetitive’ inhibition. Results showed the high revalorization potential of strawberry by-products as a low-cost source of polyphenols, with antioxidant and anti-browning effects.
Fil: Villamil Galindo, Johan Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
Fil: Van de Velde, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
Fil: Piagentini, Andrea. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
Materia
ANTIOXIDANT CAPACITY
BY-PRODUCT REVALORIZATION
POLYPHENOL OXIDASE INHIBITION
POLYPHENOLS
ULTRASOUND-ASSISTED EXTRACTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/174438

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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Extracts from strawberry by-products rich in phenolic compounds reduce the activity of apple polyphenol oxidaseVillamil Galindo, Johan EstebanVan de Velde, FrancoPiagentini, AndreaANTIOXIDANT CAPACITYBY-PRODUCT REVALORIZATIONPOLYPHENOL OXIDASE INHIBITIONPOLYPHENOLSULTRASOUND-ASSISTED EXTRACTIONhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2The ultrasound-assisted extraction of phenolic compounds from strawberry by-products was studied varying the solvent type (water, ethanol 80%, methanol 80%, and acetone 80%), the formic acid concentration (0 and 0.5%), and the number of extraction steps (1 and 2). Total phenolic and total flavonoid compounds were determined spectrophotometrically and analyzed by PAD-HPLC. The antioxidant capacity (DPPH and FRAP assays) and the ability of extracts to inhibit apple (Malus domestica cv. ‘Red Delicious’) polyphenol oxidase (PPO) were also investigated. Extracts with acidified methanol in two-steps yielded the highest phenolic compound concentration (15.01 g/kg), and the highest antioxidant capacity. Agrimoniin was the major polyphenol found, and the extraction with acetone in two-steps produced the highest yield (2.45 g/kg). This ellagitannin was the only polyphenol that correlated (R2 > 0.80, p < 0.05) with the antioxidant capacity. Water and ethanol showed the lowest phenolic compound yields. However, extraction with these green solvents (water or ethanol) in two-steps showed polyphenol contents similar to those obtained with methanol or acetone in one-step (≈9 g/kg). Additionally, extracted polyphenols (0.24 g/L) produced 30% apple PPO inhibition, in a reversible ‘uncompetitive’ inhibition. Results showed the high revalorization potential of strawberry by-products as a low-cost source of polyphenols, with antioxidant and anti-browning effects.Fil: Villamil Galindo, Johan Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; ArgentinaFil: Van de Velde, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; ArgentinaFil: Piagentini, Andrea. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; ArgentinaElsevier Science2020-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/174438Villamil Galindo, Johan Esteban; Van de Velde, Franco; Piagentini, Andrea; Extracts from strawberry by-products rich in phenolic compounds reduce the activity of apple polyphenol oxidase; Elsevier Science; LWT - Food Science and Technology; 133; 11-2020; 1-360023-6438CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0023643820310860info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2020.110097info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:12Zoai:ri.conicet.gov.ar:11336/174438instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:12.989CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Extracts from strawberry by-products rich in phenolic compounds reduce the activity of apple polyphenol oxidase
title Extracts from strawberry by-products rich in phenolic compounds reduce the activity of apple polyphenol oxidase
spellingShingle Extracts from strawberry by-products rich in phenolic compounds reduce the activity of apple polyphenol oxidase
Villamil Galindo, Johan Esteban
ANTIOXIDANT CAPACITY
BY-PRODUCT REVALORIZATION
POLYPHENOL OXIDASE INHIBITION
POLYPHENOLS
ULTRASOUND-ASSISTED EXTRACTION
title_short Extracts from strawberry by-products rich in phenolic compounds reduce the activity of apple polyphenol oxidase
title_full Extracts from strawberry by-products rich in phenolic compounds reduce the activity of apple polyphenol oxidase
title_fullStr Extracts from strawberry by-products rich in phenolic compounds reduce the activity of apple polyphenol oxidase
title_full_unstemmed Extracts from strawberry by-products rich in phenolic compounds reduce the activity of apple polyphenol oxidase
title_sort Extracts from strawberry by-products rich in phenolic compounds reduce the activity of apple polyphenol oxidase
dc.creator.none.fl_str_mv Villamil Galindo, Johan Esteban
Van de Velde, Franco
Piagentini, Andrea
author Villamil Galindo, Johan Esteban
author_facet Villamil Galindo, Johan Esteban
Van de Velde, Franco
Piagentini, Andrea
author_role author
author2 Van de Velde, Franco
Piagentini, Andrea
author2_role author
author
dc.subject.none.fl_str_mv ANTIOXIDANT CAPACITY
BY-PRODUCT REVALORIZATION
POLYPHENOL OXIDASE INHIBITION
POLYPHENOLS
ULTRASOUND-ASSISTED EXTRACTION
topic ANTIOXIDANT CAPACITY
BY-PRODUCT REVALORIZATION
POLYPHENOL OXIDASE INHIBITION
POLYPHENOLS
ULTRASOUND-ASSISTED EXTRACTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The ultrasound-assisted extraction of phenolic compounds from strawberry by-products was studied varying the solvent type (water, ethanol 80%, methanol 80%, and acetone 80%), the formic acid concentration (0 and 0.5%), and the number of extraction steps (1 and 2). Total phenolic and total flavonoid compounds were determined spectrophotometrically and analyzed by PAD-HPLC. The antioxidant capacity (DPPH and FRAP assays) and the ability of extracts to inhibit apple (Malus domestica cv. ‘Red Delicious’) polyphenol oxidase (PPO) were also investigated. Extracts with acidified methanol in two-steps yielded the highest phenolic compound concentration (15.01 g/kg), and the highest antioxidant capacity. Agrimoniin was the major polyphenol found, and the extraction with acetone in two-steps produced the highest yield (2.45 g/kg). This ellagitannin was the only polyphenol that correlated (R2 > 0.80, p < 0.05) with the antioxidant capacity. Water and ethanol showed the lowest phenolic compound yields. However, extraction with these green solvents (water or ethanol) in two-steps showed polyphenol contents similar to those obtained with methanol or acetone in one-step (≈9 g/kg). Additionally, extracted polyphenols (0.24 g/L) produced 30% apple PPO inhibition, in a reversible ‘uncompetitive’ inhibition. Results showed the high revalorization potential of strawberry by-products as a low-cost source of polyphenols, with antioxidant and anti-browning effects.
Fil: Villamil Galindo, Johan Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
Fil: Van de Velde, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
Fil: Piagentini, Andrea. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
description The ultrasound-assisted extraction of phenolic compounds from strawberry by-products was studied varying the solvent type (water, ethanol 80%, methanol 80%, and acetone 80%), the formic acid concentration (0 and 0.5%), and the number of extraction steps (1 and 2). Total phenolic and total flavonoid compounds were determined spectrophotometrically and analyzed by PAD-HPLC. The antioxidant capacity (DPPH and FRAP assays) and the ability of extracts to inhibit apple (Malus domestica cv. ‘Red Delicious’) polyphenol oxidase (PPO) were also investigated. Extracts with acidified methanol in two-steps yielded the highest phenolic compound concentration (15.01 g/kg), and the highest antioxidant capacity. Agrimoniin was the major polyphenol found, and the extraction with acetone in two-steps produced the highest yield (2.45 g/kg). This ellagitannin was the only polyphenol that correlated (R2 > 0.80, p < 0.05) with the antioxidant capacity. Water and ethanol showed the lowest phenolic compound yields. However, extraction with these green solvents (water or ethanol) in two-steps showed polyphenol contents similar to those obtained with methanol or acetone in one-step (≈9 g/kg). Additionally, extracted polyphenols (0.24 g/L) produced 30% apple PPO inhibition, in a reversible ‘uncompetitive’ inhibition. Results showed the high revalorization potential of strawberry by-products as a low-cost source of polyphenols, with antioxidant and anti-browning effects.
publishDate 2020
dc.date.none.fl_str_mv 2020-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/174438
Villamil Galindo, Johan Esteban; Van de Velde, Franco; Piagentini, Andrea; Extracts from strawberry by-products rich in phenolic compounds reduce the activity of apple polyphenol oxidase; Elsevier Science; LWT - Food Science and Technology; 133; 11-2020; 1-36
0023-6438
CONICET Digital
CONICET
url http://hdl.handle.net/11336/174438
identifier_str_mv Villamil Galindo, Johan Esteban; Van de Velde, Franco; Piagentini, Andrea; Extracts from strawberry by-products rich in phenolic compounds reduce the activity of apple polyphenol oxidase; Elsevier Science; LWT - Food Science and Technology; 133; 11-2020; 1-36
0023-6438
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0023643820310860
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2020.110097
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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