Ethanol consumption enhances periodontal inflammatory markers in rats

Autores
Dantas, Aline Maia; Mohn, Claudia Ester; Burdet, Berenice; Zorrilla Zubilete, María Aurelia; Mandalunis, Patricia Mónica; Elverdin, Juan Carlos; Fernández Solari, Jose Javier
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
OBJECTIVE: The aim of this study was to assess the short term effect of ethanol administration on periodontal disease in rats. DESIGN: Rats received either ethanol 2g/kg or water by gastric gavage twice a day. On the fifth day ligatures were tied around the molars of half of the rats to induce periodontitis. After 7days gingival tissue was removed and assayed for inflammatory markers. Finally, hemi-mandibles were extracted to evaluate bone loss by histomorphometrical techniques. RESULTS: The experimental periodontitis increased significantly the mRNA expression (p<0.001) and activity (p<0.001) of inducible nitric oxide synthase (iNOS) in the gingival tissue, whilst short time ethanol administration increased iNOS activity (p<0.05) and produced an additive effect on iNOS mRNA expression augmented by periodontitis (p<0.01). The short time ethanol administration also potentiated the periodontitis stimulatory effect on the mRNA expression of interleukin (IL)-1β (p<0.01 and p<0.001, in semi-quantitative and real time PCR, respectively) and on the height of periodontal ligament (p<0.05). However, the ligature-induced periodontitis, but not ethanol administration, increased the prostaglandin E(2) content (p<0.05) and, diminished the alveolar bone volume (p<0.05), as compared to sham rats. CONCLUSION: The present results suggest that ethanol consumption could represent a risk indicator for periodontal disease since augments the expression of inflammatory markers, in healthy rats, and increases them, at short term, during the illness. However, scale longitudinal investigation and more case-control studies are needed to confirm this statement.
Fil: Dantas, Aline Maia. Universidade de Sao Paulo; Brasil. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Mohn, Claudia Ester. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Burdet, Berenice. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Zorrilla Zubilete, María Aurelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Mandalunis, Patricia Mónica. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Histología y Embriología; Argentina
Fil: Elverdin, Juan Carlos. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Fernández Solari, Jose Javier. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Experimental Periodontitis
Prostaglandin E
Interleukin 1β
Nitric Oxide
Bone Loss
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16304
Acceder
id CONICETDig_ef09680c66bb79b1a3a2ed6b916de2ec
oai_identifier_str oai:ri.conicet.gov.ar:11336/16304
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ethanol consumption enhances periodontal inflammatory markers in ratsDantas, Aline MaiaMohn, Claudia EsterBurdet, BereniceZorrilla Zubilete, María AureliaMandalunis, Patricia MónicaElverdin, Juan CarlosFernández Solari, Jose JavierExperimental PeriodontitisProstaglandin EInterleukin 1βNitric OxideBone Losshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3OBJECTIVE: The aim of this study was to assess the short term effect of ethanol administration on periodontal disease in rats. DESIGN: Rats received either ethanol 2g/kg or water by gastric gavage twice a day. On the fifth day ligatures were tied around the molars of half of the rats to induce periodontitis. After 7days gingival tissue was removed and assayed for inflammatory markers. Finally, hemi-mandibles were extracted to evaluate bone loss by histomorphometrical techniques. RESULTS: The experimental periodontitis increased significantly the mRNA expression (p<0.001) and activity (p<0.001) of inducible nitric oxide synthase (iNOS) in the gingival tissue, whilst short time ethanol administration increased iNOS activity (p<0.05) and produced an additive effect on iNOS mRNA expression augmented by periodontitis (p<0.01). The short time ethanol administration also potentiated the periodontitis stimulatory effect on the mRNA expression of interleukin (IL)-1β (p<0.01 and p<0.001, in semi-quantitative and real time PCR, respectively) and on the height of periodontal ligament (p<0.05). However, the ligature-induced periodontitis, but not ethanol administration, increased the prostaglandin E(2) content (p<0.05) and, diminished the alveolar bone volume (p<0.05), as compared to sham rats. CONCLUSION: The present results suggest that ethanol consumption could represent a risk indicator for periodontal disease since augments the expression of inflammatory markers, in healthy rats, and increases them, at short term, during the illness. However, scale longitudinal investigation and more case-control studies are needed to confirm this statement.Fil: Dantas, Aline Maia. Universidade de Sao Paulo; Brasil. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Mohn, Claudia Ester. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Burdet, Berenice. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Zorrilla Zubilete, María Aurelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Mandalunis, Patricia Mónica. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Histología y Embriología; ArgentinaFil: Elverdin, Juan Carlos. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Fernández Solari, Jose Javier. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16304Dantas, Aline Maia; Mohn, Claudia Ester; Burdet, Berenice; Zorrilla Zubilete, María Aurelia; Mandalunis, Patricia Mónica; et al.; Ethanol consumption enhances periodontal inflammatory markers in rats; Elsevier; Archives of Oral Biology; 57; 9; 9-2012; 1211-12170003-9969enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.archoralbio.2012.02.008info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0003996912000568info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:14:48Zoai:ri.conicet.gov.ar:11336/16304instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:14:48.376CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ethanol consumption enhances periodontal inflammatory markers in rats
title Ethanol consumption enhances periodontal inflammatory markers in rats
spellingShingle Ethanol consumption enhances periodontal inflammatory markers in rats
Dantas, Aline Maia
Experimental Periodontitis
Prostaglandin E
Interleukin 1β
Nitric Oxide
Bone Loss
title_short Ethanol consumption enhances periodontal inflammatory markers in rats
title_full Ethanol consumption enhances periodontal inflammatory markers in rats
title_fullStr Ethanol consumption enhances periodontal inflammatory markers in rats
title_full_unstemmed Ethanol consumption enhances periodontal inflammatory markers in rats
title_sort Ethanol consumption enhances periodontal inflammatory markers in rats
dc.creator.none.fl_str_mv Dantas, Aline Maia
Mohn, Claudia Ester
Burdet, Berenice
Zorrilla Zubilete, María Aurelia
Mandalunis, Patricia Mónica
Elverdin, Juan Carlos
Fernández Solari, Jose Javier
author Dantas, Aline Maia
author_facet Dantas, Aline Maia
Mohn, Claudia Ester
Burdet, Berenice
Zorrilla Zubilete, María Aurelia
Mandalunis, Patricia Mónica
Elverdin, Juan Carlos
Fernández Solari, Jose Javier
author_role author
author2 Mohn, Claudia Ester
Burdet, Berenice
Zorrilla Zubilete, María Aurelia
Mandalunis, Patricia Mónica
Elverdin, Juan Carlos
Fernández Solari, Jose Javier
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Experimental Periodontitis
Prostaglandin E
Interleukin 1β
Nitric Oxide
Bone Loss
topic Experimental Periodontitis
Prostaglandin E
Interleukin 1β
Nitric Oxide
Bone Loss
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv OBJECTIVE: The aim of this study was to assess the short term effect of ethanol administration on periodontal disease in rats. DESIGN: Rats received either ethanol 2g/kg or water by gastric gavage twice a day. On the fifth day ligatures were tied around the molars of half of the rats to induce periodontitis. After 7days gingival tissue was removed and assayed for inflammatory markers. Finally, hemi-mandibles were extracted to evaluate bone loss by histomorphometrical techniques. RESULTS: The experimental periodontitis increased significantly the mRNA expression (p<0.001) and activity (p<0.001) of inducible nitric oxide synthase (iNOS) in the gingival tissue, whilst short time ethanol administration increased iNOS activity (p<0.05) and produced an additive effect on iNOS mRNA expression augmented by periodontitis (p<0.01). The short time ethanol administration also potentiated the periodontitis stimulatory effect on the mRNA expression of interleukin (IL)-1β (p<0.01 and p<0.001, in semi-quantitative and real time PCR, respectively) and on the height of periodontal ligament (p<0.05). However, the ligature-induced periodontitis, but not ethanol administration, increased the prostaglandin E(2) content (p<0.05) and, diminished the alveolar bone volume (p<0.05), as compared to sham rats. CONCLUSION: The present results suggest that ethanol consumption could represent a risk indicator for periodontal disease since augments the expression of inflammatory markers, in healthy rats, and increases them, at short term, during the illness. However, scale longitudinal investigation and more case-control studies are needed to confirm this statement.
Fil: Dantas, Aline Maia. Universidade de Sao Paulo; Brasil. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Mohn, Claudia Ester. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Burdet, Berenice. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Zorrilla Zubilete, María Aurelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Mandalunis, Patricia Mónica. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Histología y Embriología; Argentina
Fil: Elverdin, Juan Carlos. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Fernández Solari, Jose Javier. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description OBJECTIVE: The aim of this study was to assess the short term effect of ethanol administration on periodontal disease in rats. DESIGN: Rats received either ethanol 2g/kg or water by gastric gavage twice a day. On the fifth day ligatures were tied around the molars of half of the rats to induce periodontitis. After 7days gingival tissue was removed and assayed for inflammatory markers. Finally, hemi-mandibles were extracted to evaluate bone loss by histomorphometrical techniques. RESULTS: The experimental periodontitis increased significantly the mRNA expression (p<0.001) and activity (p<0.001) of inducible nitric oxide synthase (iNOS) in the gingival tissue, whilst short time ethanol administration increased iNOS activity (p<0.05) and produced an additive effect on iNOS mRNA expression augmented by periodontitis (p<0.01). The short time ethanol administration also potentiated the periodontitis stimulatory effect on the mRNA expression of interleukin (IL)-1β (p<0.01 and p<0.001, in semi-quantitative and real time PCR, respectively) and on the height of periodontal ligament (p<0.05). However, the ligature-induced periodontitis, but not ethanol administration, increased the prostaglandin E(2) content (p<0.05) and, diminished the alveolar bone volume (p<0.05), as compared to sham rats. CONCLUSION: The present results suggest that ethanol consumption could represent a risk indicator for periodontal disease since augments the expression of inflammatory markers, in healthy rats, and increases them, at short term, during the illness. However, scale longitudinal investigation and more case-control studies are needed to confirm this statement.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16304
Dantas, Aline Maia; Mohn, Claudia Ester; Burdet, Berenice; Zorrilla Zubilete, María Aurelia; Mandalunis, Patricia Mónica; et al.; Ethanol consumption enhances periodontal inflammatory markers in rats; Elsevier; Archives of Oral Biology; 57; 9; 9-2012; 1211-1217
0003-9969
url http://hdl.handle.net/11336/16304
identifier_str_mv Dantas, Aline Maia; Mohn, Claudia Ester; Burdet, Berenice; Zorrilla Zubilete, María Aurelia; Mandalunis, Patricia Mónica; et al.; Ethanol consumption enhances periodontal inflammatory markers in rats; Elsevier; Archives of Oral Biology; 57; 9; 9-2012; 1211-1217
0003-9969
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.archoralbio.2012.02.008
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0003996912000568
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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