Chronic exposure to low-dose of X-rays induces DNA double-strand breaks in mammalian cells

Autores
Grillo, Claudia Alejandra; Güerci, Alba Mabel
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The purpose of present study is to evaluate the induction of clastogenic effect after chronic exposure to low dose of ionising radiation in both CHO-K1 cells and in their DNA Double-Strand Breaks (DSBs)-repair deficient mutant xrs-5. Cells were cultured for 14 dilution cycles and grown to confluency. Radiation treatment was performed once per cycle with 10 mGy X-rays to carry out the structural chromosome aberration test. The results showed a significant increase in the frequency of achromatic lesions (p < 0.001) in CHO-K1 cells. In addition, xrs-5 cells showed an increase of chromatid and chromosome-type aberrations (p < 0.001). Sequential exposure produced a level of DNA damage that is amplified through successive cycles triggering the efficient repair of DSBs. The increased damage in xrs-5 cells provides evidence that the non-homologous end-joining repair of DNA DSBs is involved after low dose X-ray exposure.
Fil: Grillo, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Güerci, Alba Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Materia
Chronic Exposure
Dna Damage
Low Dose Radiation
Mammalian Cells
Repair-Deficient Mutant Cells
Serial Effect of Radiation
Structural Chromosome Aberration Test
X-Ray
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/78877
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/78877
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chronic exposure to low-dose of X-rays induces DNA double-strand breaks in mammalian cellsGrillo, Claudia AlejandraGüerci, Alba MabelChronic ExposureDna DamageLow Dose RadiationMammalian CellsRepair-Deficient Mutant CellsSerial Effect of RadiationStructural Chromosome Aberration TestX-Rayhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The purpose of present study is to evaluate the induction of clastogenic effect after chronic exposure to low dose of ionising radiation in both CHO-K1 cells and in their DNA Double-Strand Breaks (DSBs)-repair deficient mutant xrs-5. Cells were cultured for 14 dilution cycles and grown to confluency. Radiation treatment was performed once per cycle with 10 mGy X-rays to carry out the structural chromosome aberration test. The results showed a significant increase in the frequency of achromatic lesions (p < 0.001) in CHO-K1 cells. In addition, xrs-5 cells showed an increase of chromatid and chromosome-type aberrations (p < 0.001). Sequential exposure produced a level of DNA damage that is amplified through successive cycles triggering the efficient repair of DSBs. The increased damage in xrs-5 cells provides evidence that the non-homologous end-joining repair of DNA DSBs is involved after low dose X-ray exposure.Fil: Grillo, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Güerci, Alba Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaInderscience Enterprises2011-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/78877Grillo, Claudia Alejandra; Güerci, Alba Mabel; Chronic exposure to low-dose of X-rays induces DNA double-strand breaks in mammalian cells; Inderscience Enterprises; International Journal of Low Radiation; 8; 3; 10-2011; 198-2131477-65451741-9190CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.inderscienceonline.com/doi/abs/10.1504/IJLR.2011.046165info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742213/info:eu-repo/semantics/altIdentifier/doi/10.1504/IJLR.2011.046165info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:14Zoai:ri.conicet.gov.ar:11336/78877instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:14.215CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chronic exposure to low-dose of X-rays induces DNA double-strand breaks in mammalian cells
title Chronic exposure to low-dose of X-rays induces DNA double-strand breaks in mammalian cells
spellingShingle Chronic exposure to low-dose of X-rays induces DNA double-strand breaks in mammalian cells
Grillo, Claudia Alejandra
Chronic Exposure
Dna Damage
Low Dose Radiation
Mammalian Cells
Repair-Deficient Mutant Cells
Serial Effect of Radiation
Structural Chromosome Aberration Test
X-Ray
title_short Chronic exposure to low-dose of X-rays induces DNA double-strand breaks in mammalian cells
title_full Chronic exposure to low-dose of X-rays induces DNA double-strand breaks in mammalian cells
title_fullStr Chronic exposure to low-dose of X-rays induces DNA double-strand breaks in mammalian cells
title_full_unstemmed Chronic exposure to low-dose of X-rays induces DNA double-strand breaks in mammalian cells
title_sort Chronic exposure to low-dose of X-rays induces DNA double-strand breaks in mammalian cells
dc.creator.none.fl_str_mv Grillo, Claudia Alejandra
Güerci, Alba Mabel
author Grillo, Claudia Alejandra
author_facet Grillo, Claudia Alejandra
Güerci, Alba Mabel
author_role author
author2 Güerci, Alba Mabel
author2_role author
dc.subject.none.fl_str_mv Chronic Exposure
Dna Damage
Low Dose Radiation
Mammalian Cells
Repair-Deficient Mutant Cells
Serial Effect of Radiation
Structural Chromosome Aberration Test
X-Ray
topic Chronic Exposure
Dna Damage
Low Dose Radiation
Mammalian Cells
Repair-Deficient Mutant Cells
Serial Effect of Radiation
Structural Chromosome Aberration Test
X-Ray
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The purpose of present study is to evaluate the induction of clastogenic effect after chronic exposure to low dose of ionising radiation in both CHO-K1 cells and in their DNA Double-Strand Breaks (DSBs)-repair deficient mutant xrs-5. Cells were cultured for 14 dilution cycles and grown to confluency. Radiation treatment was performed once per cycle with 10 mGy X-rays to carry out the structural chromosome aberration test. The results showed a significant increase in the frequency of achromatic lesions (p < 0.001) in CHO-K1 cells. In addition, xrs-5 cells showed an increase of chromatid and chromosome-type aberrations (p < 0.001). Sequential exposure produced a level of DNA damage that is amplified through successive cycles triggering the efficient repair of DSBs. The increased damage in xrs-5 cells provides evidence that the non-homologous end-joining repair of DNA DSBs is involved after low dose X-ray exposure.
Fil: Grillo, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Güerci, Alba Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
description The purpose of present study is to evaluate the induction of clastogenic effect after chronic exposure to low dose of ionising radiation in both CHO-K1 cells and in their DNA Double-Strand Breaks (DSBs)-repair deficient mutant xrs-5. Cells were cultured for 14 dilution cycles and grown to confluency. Radiation treatment was performed once per cycle with 10 mGy X-rays to carry out the structural chromosome aberration test. The results showed a significant increase in the frequency of achromatic lesions (p < 0.001) in CHO-K1 cells. In addition, xrs-5 cells showed an increase of chromatid and chromosome-type aberrations (p < 0.001). Sequential exposure produced a level of DNA damage that is amplified through successive cycles triggering the efficient repair of DSBs. The increased damage in xrs-5 cells provides evidence that the non-homologous end-joining repair of DNA DSBs is involved after low dose X-ray exposure.
publishDate 2011
dc.date.none.fl_str_mv 2011-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/78877
Grillo, Claudia Alejandra; Güerci, Alba Mabel; Chronic exposure to low-dose of X-rays induces DNA double-strand breaks in mammalian cells; Inderscience Enterprises; International Journal of Low Radiation; 8; 3; 10-2011; 198-213
1477-6545
1741-9190
CONICET Digital
CONICET
url http://hdl.handle.net/11336/78877
identifier_str_mv Grillo, Claudia Alejandra; Güerci, Alba Mabel; Chronic exposure to low-dose of X-rays induces DNA double-strand breaks in mammalian cells; Inderscience Enterprises; International Journal of Low Radiation; 8; 3; 10-2011; 198-213
1477-6545
1741-9190
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.inderscienceonline.com/doi/abs/10.1504/IJLR.2011.046165
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742213/
info:eu-repo/semantics/altIdentifier/doi/10.1504/IJLR.2011.046165
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Inderscience Enterprises
publisher.none.fl_str_mv Inderscience Enterprises
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613628022489088
score 13.070432

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