Building an experimental nutritional model of obesity. Evaluation of anthropometrical and nutritional parameters

Autores
Alfonso, Javier Oscar; Lopez, Mariana; Delgado, Silvia Marcela; Navigatore Fonzo, Lorena Silvina; Anzulovich Miranda, Ana Cecilia
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The etiology of obesity is multifactorial, and includes genetic, environmental, and dietary factors, where hypercaloric diets play a central role in the development of the disease. It is known that obesity in adulthood can increase the risk of suffering neurodegenerative diseases. As a part of an institutional project that studies obesity as a base disease for the development of chronic age-associated diseases and the search for early biomarkers with predictive potential, one of our general objectives is to establish a nutritional model of obesity in rat. Particularly, the objective of this work was to evaluate the effects of a high saturated fat diet on different anthropometric and nutritional parameters. Male Wistar rats weaned at 21 days of age were fed with a normocaloric diet (ND) containing 366 kcal from lipids/kg diet. At 2 months old, they were randomly separated and fed with the NC diet (Control group) and a high saturated fat diet containing 1570.7 kcal from margarine/kg diet (HFD group) for the following 14 weeks. Animals were maintained under 12 h light:12 h dark and 22–24°C conditions, with food and water ad libitum. The anthropometric profile included the evaluation of food intake, body weight, body mass index (BMI), weight gain, dietary consumption, and Lee index, throughout the entire treatment period. The following nutritional parameters were also calculated: energy-intake, and feed efficiency. Statistical differences between groups and throughout the treatment period were analyzed by two-way ANOVA, followed by Bonferroni post-hoc test, with P < 0.05 to confirm significant differences between groups and weeks. Our results show that feeding HFD resulted in significant increases in the following anthropometric parameters: body weight (P < 0.001 from the 9th to the 21st week), BMI (P < 0.05 from the 14th to the 22nd week), weight gain (P < 0.05 from the 15th to the 22nd week) as well as in the nutritional parameter: energy-intake from lipids (P < 0.001 from the 9th to the 22nd week). We did not observe significant changes in food intake, Lee index, feed efficiency nor in the total energy intake. Thus, we could conclude that a high saturated fat, from margarine, diet modifies key anthropometrical and nutritional parameters, and it could be used to establish a nutritional model of obesity in rat.
Fil: Alfonso, Javier Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Lopez, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Delgado, Silvia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Navigatore Fonzo, Lorena Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Anzulovich Miranda, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
IV Reunión Conjunta de Sociedades de Biología de la República Argentina: “Nuevas Evidencias y Cambios de Paradigmas en Ciencias Biológicas”
Argentina
Sociedad de Biología de Cuyo
Universidad Nacional de Cuyo
Sociedad Argentina de Biología
Sociedad de Biología de Córdoba
Sociedad de Biología de Rosario
Asociación de Biología de Tucumán
Honorable Legislatura de la Provincia de Mendoza
Sociedad Chilena de Reproducción y Desarrollo
Ministerio de Salud Provincia de Mendoza
Gobierno de la Provincia de Mendoza
Materia
NUTRITIONAL MODEL
OBESITY
RAT
ANTROPOMETRIC PARAMETERS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/192253

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network_name_str CONICET Digital (CONICET)
spelling Building an experimental nutritional model of obesity. Evaluation of anthropometrical and nutritional parametersAlfonso, Javier OscarLopez, MarianaDelgado, Silvia MarcelaNavigatore Fonzo, Lorena SilvinaAnzulovich Miranda, Ana CeciliaNUTRITIONAL MODELOBESITYRATANTROPOMETRIC PARAMETERShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The etiology of obesity is multifactorial, and includes genetic, environmental, and dietary factors, where hypercaloric diets play a central role in the development of the disease. It is known that obesity in adulthood can increase the risk of suffering neurodegenerative diseases. As a part of an institutional project that studies obesity as a base disease for the development of chronic age-associated diseases and the search for early biomarkers with predictive potential, one of our general objectives is to establish a nutritional model of obesity in rat. Particularly, the objective of this work was to evaluate the effects of a high saturated fat diet on different anthropometric and nutritional parameters. Male Wistar rats weaned at 21 days of age were fed with a normocaloric diet (ND) containing 366 kcal from lipids/kg diet. At 2 months old, they were randomly separated and fed with the NC diet (Control group) and a high saturated fat diet containing 1570.7 kcal from margarine/kg diet (HFD group) for the following 14 weeks. Animals were maintained under 12 h light:12 h dark and 22–24°C conditions, with food and water ad libitum. The anthropometric profile included the evaluation of food intake, body weight, body mass index (BMI), weight gain, dietary consumption, and Lee index, throughout the entire treatment period. The following nutritional parameters were also calculated: energy-intake, and feed efficiency. Statistical differences between groups and throughout the treatment period were analyzed by two-way ANOVA, followed by Bonferroni post-hoc test, with P < 0.05 to confirm significant differences between groups and weeks. Our results show that feeding HFD resulted in significant increases in the following anthropometric parameters: body weight (P < 0.001 from the 9th to the 21st week), BMI (P < 0.05 from the 14th to the 22nd week), weight gain (P < 0.05 from the 15th to the 22nd week) as well as in the nutritional parameter: energy-intake from lipids (P < 0.001 from the 9th to the 22nd week). We did not observe significant changes in food intake, Lee index, feed efficiency nor in the total energy intake. Thus, we could conclude that a high saturated fat, from margarine, diet modifies key anthropometrical and nutritional parameters, and it could be used to establish a nutritional model of obesity in rat.Fil: Alfonso, Javier Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Lopez, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Delgado, Silvia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Navigatore Fonzo, Lorena Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Anzulovich Miranda, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaIV Reunión Conjunta de Sociedades de Biología de la República Argentina: “Nuevas Evidencias y Cambios de Paradigmas en Ciencias Biológicas”ArgentinaSociedad de Biología de CuyoUniversidad Nacional de CuyoSociedad Argentina de BiologíaSociedad de Biología de CórdobaSociedad de Biología de RosarioAsociación de Biología de TucumánHonorable Legislatura de la Provincia de MendozaSociedad Chilena de Reproducción y DesarrolloMinisterio de Salud Provincia de MendozaGobierno de la Provincia de MendozaTech Science PressAjmat, María TeresaBorioli, Graciela2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/192253Building an experimental nutritional model of obesity. Evaluation of anthropometrical and nutritional parameters; IV Reunión Conjunta de Sociedades de Biología de la República Argentina: “Nuevas Evidencias y Cambios de Paradigmas en Ciencias Biológicas”; Argentina; 2020; 104-1040327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v45nSuppl.3/44000info:eu-repo/semantics/altIdentifier/url/https://www.biologia.org.ar/eventos/iv-reunion-conjunta-de-sociedades-de-biologia-de-la-republica-argentina/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-03-31T14:44:05Zoai:ri.conicet.gov.ar:11336/192253instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-03-31 14:44:05.747CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Building an experimental nutritional model of obesity. Evaluation of anthropometrical and nutritional parameters
title Building an experimental nutritional model of obesity. Evaluation of anthropometrical and nutritional parameters
spellingShingle Building an experimental nutritional model of obesity. Evaluation of anthropometrical and nutritional parameters
Alfonso, Javier Oscar
NUTRITIONAL MODEL
OBESITY
RAT
ANTROPOMETRIC PARAMETERS
title_short Building an experimental nutritional model of obesity. Evaluation of anthropometrical and nutritional parameters
title_full Building an experimental nutritional model of obesity. Evaluation of anthropometrical and nutritional parameters
title_fullStr Building an experimental nutritional model of obesity. Evaluation of anthropometrical and nutritional parameters
title_full_unstemmed Building an experimental nutritional model of obesity. Evaluation of anthropometrical and nutritional parameters
title_sort Building an experimental nutritional model of obesity. Evaluation of anthropometrical and nutritional parameters
dc.creator.none.fl_str_mv Alfonso, Javier Oscar
Lopez, Mariana
Delgado, Silvia Marcela
Navigatore Fonzo, Lorena Silvina
Anzulovich Miranda, Ana Cecilia
author Alfonso, Javier Oscar
author_facet Alfonso, Javier Oscar
Lopez, Mariana
Delgado, Silvia Marcela
Navigatore Fonzo, Lorena Silvina
Anzulovich Miranda, Ana Cecilia
author_role author
author2 Lopez, Mariana
Delgado, Silvia Marcela
Navigatore Fonzo, Lorena Silvina
Anzulovich Miranda, Ana Cecilia
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ajmat, María Teresa
Borioli, Graciela
dc.subject.none.fl_str_mv NUTRITIONAL MODEL
OBESITY
RAT
ANTROPOMETRIC PARAMETERS
topic NUTRITIONAL MODEL
OBESITY
RAT
ANTROPOMETRIC PARAMETERS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The etiology of obesity is multifactorial, and includes genetic, environmental, and dietary factors, where hypercaloric diets play a central role in the development of the disease. It is known that obesity in adulthood can increase the risk of suffering neurodegenerative diseases. As a part of an institutional project that studies obesity as a base disease for the development of chronic age-associated diseases and the search for early biomarkers with predictive potential, one of our general objectives is to establish a nutritional model of obesity in rat. Particularly, the objective of this work was to evaluate the effects of a high saturated fat diet on different anthropometric and nutritional parameters. Male Wistar rats weaned at 21 days of age were fed with a normocaloric diet (ND) containing 366 kcal from lipids/kg diet. At 2 months old, they were randomly separated and fed with the NC diet (Control group) and a high saturated fat diet containing 1570.7 kcal from margarine/kg diet (HFD group) for the following 14 weeks. Animals were maintained under 12 h light:12 h dark and 22–24°C conditions, with food and water ad libitum. The anthropometric profile included the evaluation of food intake, body weight, body mass index (BMI), weight gain, dietary consumption, and Lee index, throughout the entire treatment period. The following nutritional parameters were also calculated: energy-intake, and feed efficiency. Statistical differences between groups and throughout the treatment period were analyzed by two-way ANOVA, followed by Bonferroni post-hoc test, with P < 0.05 to confirm significant differences between groups and weeks. Our results show that feeding HFD resulted in significant increases in the following anthropometric parameters: body weight (P < 0.001 from the 9th to the 21st week), BMI (P < 0.05 from the 14th to the 22nd week), weight gain (P < 0.05 from the 15th to the 22nd week) as well as in the nutritional parameter: energy-intake from lipids (P < 0.001 from the 9th to the 22nd week). We did not observe significant changes in food intake, Lee index, feed efficiency nor in the total energy intake. Thus, we could conclude that a high saturated fat, from margarine, diet modifies key anthropometrical and nutritional parameters, and it could be used to establish a nutritional model of obesity in rat.
Fil: Alfonso, Javier Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Lopez, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Delgado, Silvia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Navigatore Fonzo, Lorena Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Anzulovich Miranda, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
IV Reunión Conjunta de Sociedades de Biología de la República Argentina: “Nuevas Evidencias y Cambios de Paradigmas en Ciencias Biológicas”
Argentina
Sociedad de Biología de Cuyo
Universidad Nacional de Cuyo
Sociedad Argentina de Biología
Sociedad de Biología de Córdoba
Sociedad de Biología de Rosario
Asociación de Biología de Tucumán
Honorable Legislatura de la Provincia de Mendoza
Sociedad Chilena de Reproducción y Desarrollo
Ministerio de Salud Provincia de Mendoza
Gobierno de la Provincia de Mendoza
description The etiology of obesity is multifactorial, and includes genetic, environmental, and dietary factors, where hypercaloric diets play a central role in the development of the disease. It is known that obesity in adulthood can increase the risk of suffering neurodegenerative diseases. As a part of an institutional project that studies obesity as a base disease for the development of chronic age-associated diseases and the search for early biomarkers with predictive potential, one of our general objectives is to establish a nutritional model of obesity in rat. Particularly, the objective of this work was to evaluate the effects of a high saturated fat diet on different anthropometric and nutritional parameters. Male Wistar rats weaned at 21 days of age were fed with a normocaloric diet (ND) containing 366 kcal from lipids/kg diet. At 2 months old, they were randomly separated and fed with the NC diet (Control group) and a high saturated fat diet containing 1570.7 kcal from margarine/kg diet (HFD group) for the following 14 weeks. Animals were maintained under 12 h light:12 h dark and 22–24°C conditions, with food and water ad libitum. The anthropometric profile included the evaluation of food intake, body weight, body mass index (BMI), weight gain, dietary consumption, and Lee index, throughout the entire treatment period. The following nutritional parameters were also calculated: energy-intake, and feed efficiency. Statistical differences between groups and throughout the treatment period were analyzed by two-way ANOVA, followed by Bonferroni post-hoc test, with P < 0.05 to confirm significant differences between groups and weeks. Our results show that feeding HFD resulted in significant increases in the following anthropometric parameters: body weight (P < 0.001 from the 9th to the 21st week), BMI (P < 0.05 from the 14th to the 22nd week), weight gain (P < 0.05 from the 15th to the 22nd week) as well as in the nutritional parameter: energy-intake from lipids (P < 0.001 from the 9th to the 22nd week). We did not observe significant changes in food intake, Lee index, feed efficiency nor in the total energy intake. Thus, we could conclude that a high saturated fat, from margarine, diet modifies key anthropometrical and nutritional parameters, and it could be used to establish a nutritional model of obesity in rat.
publishDate 2021
dc.date.none.fl_str_mv 2021
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status_str publishedVersion
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/192253
Building an experimental nutritional model of obesity. Evaluation of anthropometrical and nutritional parameters; IV Reunión Conjunta de Sociedades de Biología de la República Argentina: “Nuevas Evidencias y Cambios de Paradigmas en Ciencias Biológicas”; Argentina; 2020; 104-104
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/192253
identifier_str_mv Building an experimental nutritional model of obesity. Evaluation of anthropometrical and nutritional parameters; IV Reunión Conjunta de Sociedades de Biología de la República Argentina: “Nuevas Evidencias y Cambios de Paradigmas en Ciencias Biológicas”; Argentina; 2020; 104-104
0327-9545
1667-5746
CONICET Digital
CONICET
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language eng
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