The developing ovary of the South American plains vizcacha, Lagostomus maximus (Mammalia, Rodentia): Massive proliferation with no sign of apoptosis-mediated germ cell attrition

Autores
Leopardo, Noelia Paola; Jensen, Cristian Federico; Willis, Miguel Alfredo; Espinosa, Maria Beatriz; Vitullo, Alfredo Daniel
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Apoptosis-dependent massive germ cell death is considered a constitutive trait of the developing mammalian ovary that eliminates 65-85% of the germinal tissue depending on the species. After birth and during adult lifetime, apoptotic activity moves from the germ cell proper to the somatic compartment, decimating germ cells through follicular atresia until the oocyte reserve is exhausted. In contrast, the South American rodent Lagostomus maximus shows suppressed apoptosis-dependent follicular atresia in the adult ovary, with continuous folliculogenesis and massive polyovulation, which finally exhausts the oocyte pool. The absence of follicular atresia in adult L. maximus might arise from a failure to move apoptosis from the germinal stratum to the somatic compartment after birth or being a constitutive trait of the ovarian tissue with no massive germ cell degeneration in the developing ovary. We tested these possibilities by analysing oogenesis, expression of germ cell-specific VASA protein, apoptotic proteins BCL2 and BAX, and DNA fragmentation by TUNEL assay in the developing ovary of L. maximus. Immunolabelling for VASA revealed a massive and widespread colonisation of the ovary and proliferation of germ cells organised in nests that disappeared at late development when folliculogenesis began. No sign of germ cell attrition was found at any time point. BCL2 remained positive throughout oogenesis, whereas BAXwas slightly detected in early development. TUNEL assay was conspicuously negative throughout the development. These results advocate for an unrestricted proliferation of germ cells, without apoptosis-driven elimination, as a constitutive trait of L. maximus ovary as opposed to what is normally found in the developing mammalian ovary.
Fil: Leopardo, Noelia Paola. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Jensen, Cristian Federico. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Willis, Miguel Alfredo. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Espinosa, Maria Beatriz. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vitullo, Alfredo Daniel. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
LAGOSTOMUS MAXIMUS
FETAL OVARY
GERM CELL
APOPTOSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/192428
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/192428
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The developing ovary of the South American plains vizcacha, Lagostomus maximus (Mammalia, Rodentia): Massive proliferation with no sign of apoptosis-mediated germ cell attritionLeopardo, Noelia PaolaJensen, Cristian FedericoWillis, Miguel AlfredoEspinosa, Maria BeatrizVitullo, Alfredo DanielLAGOSTOMUS MAXIMUSFETAL OVARYGERM CELLAPOPTOSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Apoptosis-dependent massive germ cell death is considered a constitutive trait of the developing mammalian ovary that eliminates 65-85% of the germinal tissue depending on the species. After birth and during adult lifetime, apoptotic activity moves from the germ cell proper to the somatic compartment, decimating germ cells through follicular atresia until the oocyte reserve is exhausted. In contrast, the South American rodent Lagostomus maximus shows suppressed apoptosis-dependent follicular atresia in the adult ovary, with continuous folliculogenesis and massive polyovulation, which finally exhausts the oocyte pool. The absence of follicular atresia in adult L. maximus might arise from a failure to move apoptosis from the germinal stratum to the somatic compartment after birth or being a constitutive trait of the ovarian tissue with no massive germ cell degeneration in the developing ovary. We tested these possibilities by analysing oogenesis, expression of germ cell-specific VASA protein, apoptotic proteins BCL2 and BAX, and DNA fragmentation by TUNEL assay in the developing ovary of L. maximus. Immunolabelling for VASA revealed a massive and widespread colonisation of the ovary and proliferation of germ cells organised in nests that disappeared at late development when folliculogenesis began. No sign of germ cell attrition was found at any time point. BCL2 remained positive throughout oogenesis, whereas BAXwas slightly detected in early development. TUNEL assay was conspicuously negative throughout the development. These results advocate for an unrestricted proliferation of germ cells, without apoptosis-driven elimination, as a constitutive trait of L. maximus ovary as opposed to what is normally found in the developing mammalian ovary.Fil: Leopardo, Noelia Paola. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Jensen, Cristian Federico. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Willis, Miguel Alfredo. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Espinosa, Maria Beatriz. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vitullo, Alfredo Daniel. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaBioScientifica2011-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/192428Leopardo, Noelia Paola; Jensen, Cristian Federico; Willis, Miguel Alfredo; Espinosa, Maria Beatriz; Vitullo, Alfredo Daniel; The developing ovary of the South American plains vizcacha, Lagostomus maximus (Mammalia, Rodentia): Massive proliferation with no sign of apoptosis-mediated germ cell attrition; BioScientifica; Reproduction; 141; 5; 5-2011; 633-6411470-1626CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/141/5/633.abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1530/REP-10-0463info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:41:35Zoai:ri.conicet.gov.ar:11336/192428instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:41:36.244CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The developing ovary of the South American plains vizcacha, Lagostomus maximus (Mammalia, Rodentia): Massive proliferation with no sign of apoptosis-mediated germ cell attrition
title The developing ovary of the South American plains vizcacha, Lagostomus maximus (Mammalia, Rodentia): Massive proliferation with no sign of apoptosis-mediated germ cell attrition
spellingShingle The developing ovary of the South American plains vizcacha, Lagostomus maximus (Mammalia, Rodentia): Massive proliferation with no sign of apoptosis-mediated germ cell attrition
Leopardo, Noelia Paola
LAGOSTOMUS MAXIMUS
FETAL OVARY
GERM CELL
APOPTOSIS
title_short The developing ovary of the South American plains vizcacha, Lagostomus maximus (Mammalia, Rodentia): Massive proliferation with no sign of apoptosis-mediated germ cell attrition
title_full The developing ovary of the South American plains vizcacha, Lagostomus maximus (Mammalia, Rodentia): Massive proliferation with no sign of apoptosis-mediated germ cell attrition
title_fullStr The developing ovary of the South American plains vizcacha, Lagostomus maximus (Mammalia, Rodentia): Massive proliferation with no sign of apoptosis-mediated germ cell attrition
title_full_unstemmed The developing ovary of the South American plains vizcacha, Lagostomus maximus (Mammalia, Rodentia): Massive proliferation with no sign of apoptosis-mediated germ cell attrition
title_sort The developing ovary of the South American plains vizcacha, Lagostomus maximus (Mammalia, Rodentia): Massive proliferation with no sign of apoptosis-mediated germ cell attrition
dc.creator.none.fl_str_mv Leopardo, Noelia Paola
Jensen, Cristian Federico
Willis, Miguel Alfredo
Espinosa, Maria Beatriz
Vitullo, Alfredo Daniel
author Leopardo, Noelia Paola
author_facet Leopardo, Noelia Paola
Jensen, Cristian Federico
Willis, Miguel Alfredo
Espinosa, Maria Beatriz
Vitullo, Alfredo Daniel
author_role author
author2 Jensen, Cristian Federico
Willis, Miguel Alfredo
Espinosa, Maria Beatriz
Vitullo, Alfredo Daniel
author2_role author
author
author
author
dc.subject.none.fl_str_mv LAGOSTOMUS MAXIMUS
FETAL OVARY
GERM CELL
APOPTOSIS
topic LAGOSTOMUS MAXIMUS
FETAL OVARY
GERM CELL
APOPTOSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Apoptosis-dependent massive germ cell death is considered a constitutive trait of the developing mammalian ovary that eliminates 65-85% of the germinal tissue depending on the species. After birth and during adult lifetime, apoptotic activity moves from the germ cell proper to the somatic compartment, decimating germ cells through follicular atresia until the oocyte reserve is exhausted. In contrast, the South American rodent Lagostomus maximus shows suppressed apoptosis-dependent follicular atresia in the adult ovary, with continuous folliculogenesis and massive polyovulation, which finally exhausts the oocyte pool. The absence of follicular atresia in adult L. maximus might arise from a failure to move apoptosis from the germinal stratum to the somatic compartment after birth or being a constitutive trait of the ovarian tissue with no massive germ cell degeneration in the developing ovary. We tested these possibilities by analysing oogenesis, expression of germ cell-specific VASA protein, apoptotic proteins BCL2 and BAX, and DNA fragmentation by TUNEL assay in the developing ovary of L. maximus. Immunolabelling for VASA revealed a massive and widespread colonisation of the ovary and proliferation of germ cells organised in nests that disappeared at late development when folliculogenesis began. No sign of germ cell attrition was found at any time point. BCL2 remained positive throughout oogenesis, whereas BAXwas slightly detected in early development. TUNEL assay was conspicuously negative throughout the development. These results advocate for an unrestricted proliferation of germ cells, without apoptosis-driven elimination, as a constitutive trait of L. maximus ovary as opposed to what is normally found in the developing mammalian ovary.
Fil: Leopardo, Noelia Paola. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Jensen, Cristian Federico. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Willis, Miguel Alfredo. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Espinosa, Maria Beatriz. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vitullo, Alfredo Daniel. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Apoptosis-dependent massive germ cell death is considered a constitutive trait of the developing mammalian ovary that eliminates 65-85% of the germinal tissue depending on the species. After birth and during adult lifetime, apoptotic activity moves from the germ cell proper to the somatic compartment, decimating germ cells through follicular atresia until the oocyte reserve is exhausted. In contrast, the South American rodent Lagostomus maximus shows suppressed apoptosis-dependent follicular atresia in the adult ovary, with continuous folliculogenesis and massive polyovulation, which finally exhausts the oocyte pool. The absence of follicular atresia in adult L. maximus might arise from a failure to move apoptosis from the germinal stratum to the somatic compartment after birth or being a constitutive trait of the ovarian tissue with no massive germ cell degeneration in the developing ovary. We tested these possibilities by analysing oogenesis, expression of germ cell-specific VASA protein, apoptotic proteins BCL2 and BAX, and DNA fragmentation by TUNEL assay in the developing ovary of L. maximus. Immunolabelling for VASA revealed a massive and widespread colonisation of the ovary and proliferation of germ cells organised in nests that disappeared at late development when folliculogenesis began. No sign of germ cell attrition was found at any time point. BCL2 remained positive throughout oogenesis, whereas BAXwas slightly detected in early development. TUNEL assay was conspicuously negative throughout the development. These results advocate for an unrestricted proliferation of germ cells, without apoptosis-driven elimination, as a constitutive trait of L. maximus ovary as opposed to what is normally found in the developing mammalian ovary.
publishDate 2011
dc.date.none.fl_str_mv 2011-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/192428
Leopardo, Noelia Paola; Jensen, Cristian Federico; Willis, Miguel Alfredo; Espinosa, Maria Beatriz; Vitullo, Alfredo Daniel; The developing ovary of the South American plains vizcacha, Lagostomus maximus (Mammalia, Rodentia): Massive proliferation with no sign of apoptosis-mediated germ cell attrition; BioScientifica; Reproduction; 141; 5; 5-2011; 633-641
1470-1626
CONICET Digital
CONICET
url http://hdl.handle.net/11336/192428
identifier_str_mv Leopardo, Noelia Paola; Jensen, Cristian Federico; Willis, Miguel Alfredo; Espinosa, Maria Beatriz; Vitullo, Alfredo Daniel; The developing ovary of the South American plains vizcacha, Lagostomus maximus (Mammalia, Rodentia): Massive proliferation with no sign of apoptosis-mediated germ cell attrition; BioScientifica; Reproduction; 141; 5; 5-2011; 633-641
1470-1626
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/141/5/633.abstract
info:eu-repo/semantics/altIdentifier/doi/10.1530/REP-10-0463
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv BioScientifica
publisher.none.fl_str_mv BioScientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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