Quantification of healthy and atretic germ cells and follicles in the developing and post-natal ovary of the South American plains vizcacha, Lagostomus maximus: evidence of continu...

Autores
Inserra, Pablo Ignacio Felipe; Leopardo, Noelia Paola; Willis, Miguel Alfredo; Freysselinard, A. L.; Vitullo, Alfredo Daniel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Female germ line in mammals is subjected to massive cell death that eliminates 60-85% of the germinal reserve by birth, and continues from birth to adult life until the exhaustion of the germinal pool. Germ cell demise occurs mainly through apoptosis by means of a biased expression in favour of pro-apoptotic members of the BCL2 gene family. In contrast, the South American plains vizcacha, Lagostomus maximus, shows as sustained expression of the anti-apoptotic BCL2 gene throughout gestation and a low incidence of apoptotic germ cells. This led to propose that, in the absence of death mechanisms other than apoptosis, female germ line should continuously increase from foetal life until after birth. We quantified all healthy germ cells and follicles in L. maximus ovaries from early foetal life to day 60 after birth using unbiased stereological methods and labelling with TUNEL assay to detect apoptosis. Healthy germ cell population increased continuously from early developing ovary reaching a 50 times higher population number by the end of gestation. TUNEL positive germ cells were <0.5% of germ cell number except at mid-gestation (3.62%). Mitotic proliferation, entrance to prophase I and primordial follicle formation occurred as overlapped processes from early pregnancy to birth. Germ cell number remained constant in early postnatal life but a remnant population of non-follicular VASA and PCNA positive germ cells still persisted at postnatal day 60. L. maximus is the first mammal so far described in which female germ line develops in the absence of constitutive massive germ cell elimination.
Fil: Inserra, Pablo Ignacio Felipe. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leopardo, Noelia Paola. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Willis, Miguel Alfredo. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Freysselinard, A. L.. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina
Fil: Vitullo, Alfredo Daniel. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Germ Cell
Ovary
Lagostomus
Apoptosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/26708
Acceder
id CONICETDig_e65345bb975cbe60ef4960f8ba1e43f1
oai_identifier_str oai:ri.conicet.gov.ar:11336/26708
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Quantification of healthy and atretic germ cells and follicles in the developing and post-natal ovary of the South American plains vizcacha, Lagostomus maximus: evidence of continuous rise of the germinal reserveInserra, Pablo Ignacio FelipeLeopardo, Noelia PaolaWillis, Miguel AlfredoFreysselinard, A. L.Vitullo, Alfredo DanielGerm CellOvaryLagostomusApoptosishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Female germ line in mammals is subjected to massive cell death that eliminates 60-85% of the germinal reserve by birth, and continues from birth to adult life until the exhaustion of the germinal pool. Germ cell demise occurs mainly through apoptosis by means of a biased expression in favour of pro-apoptotic members of the BCL2 gene family. In contrast, the South American plains vizcacha, Lagostomus maximus, shows as sustained expression of the anti-apoptotic BCL2 gene throughout gestation and a low incidence of apoptotic germ cells. This led to propose that, in the absence of death mechanisms other than apoptosis, female germ line should continuously increase from foetal life until after birth. We quantified all healthy germ cells and follicles in L. maximus ovaries from early foetal life to day 60 after birth using unbiased stereological methods and labelling with TUNEL assay to detect apoptosis. Healthy germ cell population increased continuously from early developing ovary reaching a 50 times higher population number by the end of gestation. TUNEL positive germ cells were <0.5% of germ cell number except at mid-gestation (3.62%). Mitotic proliferation, entrance to prophase I and primordial follicle formation occurred as overlapped processes from early pregnancy to birth. Germ cell number remained constant in early postnatal life but a remnant population of non-follicular VASA and PCNA positive germ cells still persisted at postnatal day 60. L. maximus is the first mammal so far described in which female germ line develops in the absence of constitutive massive germ cell elimination.Fil: Inserra, Pablo Ignacio Felipe. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Leopardo, Noelia Paola. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Willis, Miguel Alfredo. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Freysselinard, A. L.. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Vitullo, Alfredo Daniel. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaBioScientifica2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26708Inserra, Pablo Ignacio Felipe; Leopardo, Noelia Paola; Willis, Miguel Alfredo; Freysselinard, A. L.; Vitullo, Alfredo Daniel; Quantification of healthy and atretic germ cells and follicles in the developing and post-natal ovary of the South American plains vizcacha, Lagostomus maximus: evidence of continuous rise of the germinal reserve; BioScientifica; Reproduction; 147; 11-2013; 199-2091470-1626CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/early/2013/11/14/REP-13-0455info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:52:51Zoai:ri.conicet.gov.ar:11336/26708instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:52:51.596CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Quantification of healthy and atretic germ cells and follicles in the developing and post-natal ovary of the South American plains vizcacha, Lagostomus maximus: evidence of continuous rise of the germinal reserve
title Quantification of healthy and atretic germ cells and follicles in the developing and post-natal ovary of the South American plains vizcacha, Lagostomus maximus: evidence of continuous rise of the germinal reserve
spellingShingle Quantification of healthy and atretic germ cells and follicles in the developing and post-natal ovary of the South American plains vizcacha, Lagostomus maximus: evidence of continuous rise of the germinal reserve
Inserra, Pablo Ignacio Felipe
Germ Cell
Ovary
Lagostomus
Apoptosis
title_short Quantification of healthy and atretic germ cells and follicles in the developing and post-natal ovary of the South American plains vizcacha, Lagostomus maximus: evidence of continuous rise of the germinal reserve
title_full Quantification of healthy and atretic germ cells and follicles in the developing and post-natal ovary of the South American plains vizcacha, Lagostomus maximus: evidence of continuous rise of the germinal reserve
title_fullStr Quantification of healthy and atretic germ cells and follicles in the developing and post-natal ovary of the South American plains vizcacha, Lagostomus maximus: evidence of continuous rise of the germinal reserve
title_full_unstemmed Quantification of healthy and atretic germ cells and follicles in the developing and post-natal ovary of the South American plains vizcacha, Lagostomus maximus: evidence of continuous rise of the germinal reserve
title_sort Quantification of healthy and atretic germ cells and follicles in the developing and post-natal ovary of the South American plains vizcacha, Lagostomus maximus: evidence of continuous rise of the germinal reserve
dc.creator.none.fl_str_mv Inserra, Pablo Ignacio Felipe
Leopardo, Noelia Paola
Willis, Miguel Alfredo
Freysselinard, A. L.
Vitullo, Alfredo Daniel
author Inserra, Pablo Ignacio Felipe
author_facet Inserra, Pablo Ignacio Felipe
Leopardo, Noelia Paola
Willis, Miguel Alfredo
Freysselinard, A. L.
Vitullo, Alfredo Daniel
author_role author
author2 Leopardo, Noelia Paola
Willis, Miguel Alfredo
Freysselinard, A. L.
Vitullo, Alfredo Daniel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Germ Cell
Ovary
Lagostomus
Apoptosis
topic Germ Cell
Ovary
Lagostomus
Apoptosis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Female germ line in mammals is subjected to massive cell death that eliminates 60-85% of the germinal reserve by birth, and continues from birth to adult life until the exhaustion of the germinal pool. Germ cell demise occurs mainly through apoptosis by means of a biased expression in favour of pro-apoptotic members of the BCL2 gene family. In contrast, the South American plains vizcacha, Lagostomus maximus, shows as sustained expression of the anti-apoptotic BCL2 gene throughout gestation and a low incidence of apoptotic germ cells. This led to propose that, in the absence of death mechanisms other than apoptosis, female germ line should continuously increase from foetal life until after birth. We quantified all healthy germ cells and follicles in L. maximus ovaries from early foetal life to day 60 after birth using unbiased stereological methods and labelling with TUNEL assay to detect apoptosis. Healthy germ cell population increased continuously from early developing ovary reaching a 50 times higher population number by the end of gestation. TUNEL positive germ cells were <0.5% of germ cell number except at mid-gestation (3.62%). Mitotic proliferation, entrance to prophase I and primordial follicle formation occurred as overlapped processes from early pregnancy to birth. Germ cell number remained constant in early postnatal life but a remnant population of non-follicular VASA and PCNA positive germ cells still persisted at postnatal day 60. L. maximus is the first mammal so far described in which female germ line develops in the absence of constitutive massive germ cell elimination.
Fil: Inserra, Pablo Ignacio Felipe. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leopardo, Noelia Paola. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Willis, Miguel Alfredo. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Freysselinard, A. L.. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina
Fil: Vitullo, Alfredo Daniel. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Female germ line in mammals is subjected to massive cell death that eliminates 60-85% of the germinal reserve by birth, and continues from birth to adult life until the exhaustion of the germinal pool. Germ cell demise occurs mainly through apoptosis by means of a biased expression in favour of pro-apoptotic members of the BCL2 gene family. In contrast, the South American plains vizcacha, Lagostomus maximus, shows as sustained expression of the anti-apoptotic BCL2 gene throughout gestation and a low incidence of apoptotic germ cells. This led to propose that, in the absence of death mechanisms other than apoptosis, female germ line should continuously increase from foetal life until after birth. We quantified all healthy germ cells and follicles in L. maximus ovaries from early foetal life to day 60 after birth using unbiased stereological methods and labelling with TUNEL assay to detect apoptosis. Healthy germ cell population increased continuously from early developing ovary reaching a 50 times higher population number by the end of gestation. TUNEL positive germ cells were <0.5% of germ cell number except at mid-gestation (3.62%). Mitotic proliferation, entrance to prophase I and primordial follicle formation occurred as overlapped processes from early pregnancy to birth. Germ cell number remained constant in early postnatal life but a remnant population of non-follicular VASA and PCNA positive germ cells still persisted at postnatal day 60. L. maximus is the first mammal so far described in which female germ line develops in the absence of constitutive massive germ cell elimination.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/26708
Inserra, Pablo Ignacio Felipe; Leopardo, Noelia Paola; Willis, Miguel Alfredo; Freysselinard, A. L.; Vitullo, Alfredo Daniel; Quantification of healthy and atretic germ cells and follicles in the developing and post-natal ovary of the South American plains vizcacha, Lagostomus maximus: evidence of continuous rise of the germinal reserve; BioScientifica; Reproduction; 147; 11-2013; 199-209
1470-1626
CONICET Digital
CONICET
url http://hdl.handle.net/11336/26708
identifier_str_mv Inserra, Pablo Ignacio Felipe; Leopardo, Noelia Paola; Willis, Miguel Alfredo; Freysselinard, A. L.; Vitullo, Alfredo Daniel; Quantification of healthy and atretic germ cells and follicles in the developing and post-natal ovary of the South American plains vizcacha, Lagostomus maximus: evidence of continuous rise of the germinal reserve; BioScientifica; Reproduction; 147; 11-2013; 199-209
1470-1626
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/early/2013/11/14/REP-13-0455
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioScientifica
publisher.none.fl_str_mv BioScientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083057589682176
score 13.22299

Publicaciones similares