A mutant ankyrin protein kinase from Medicago sativa affects Arabidopsis adventitious roots

Autores
Chinchilla, Delphine; Frugier, Florian; Raices, Marcela; Merchan, Francisco; Giammaria, Veronica; Gargantini, Pablo Ruben; Gonzalez Rizzo, Silvina; Crespi, Martin; Ulloa, Rita Maria
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A family of plant kinases containing ankyrin-repeats, the Ankyrin-Protein Kinases (APKs), shows structural resemblance to mammalian Integrin-Linked Kinases (ILKs), key regulators of mammalian cell adhesion. MsAPK1 expression is induced by osmotic stress in roots of Medicago sativa (L.) plants. The Escherichia coli-purified MsAPK1 could only phosphorylate tubulin among a variety of substrates and the enzymatic activity was strictly dependent on Mn2+. MsAPK1 is highly related to two APK genes in Arabidopsis thaliana (L.), AtAPK1 and AtAPK2. Promoter-GUS fusions assays revealed that the Arabidopsis APK genes show distinct expression patterns in roots and hypocotyls. Although Medicago truncatula (L.) plants affected in MsAPK1 expression could not be obtained using in vitro regeneration, A. thaliana plants expressing MsAPK1 or a mutant MsAPK1 protein, in which the conserved aspartate 315 of the kinase catalytic domain was replaced by asparagines (DN-lines), developed normally. The DN mutant lines showed increased capacity to develop adventitious roots when compared with control or MsAPK1-expressing plants. APK-mediated signalling may therefore link perception of external abiotic signals and the microtubule cytoskeleton, and influence adventitious root development.
Fil: Chinchilla, Delphine. Institut des Sciences du Végétal; Francia. Universidad de Basilea; Suiza
Fil: Frugier, Florian. Institut des Sciences du Végétal; Francia
Fil: Raices, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. The Salk Institute for Biological Studies; Estados Unidos
Fil: Merchan, Francisco. Institut des Sciences du Végétal; Francia
Fil: Giammaria, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Gargantini, Pablo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Gonzalez Rizzo, Silvina. Institut des Sciences du Végétal; Francia
Fil: Crespi, Martin. Institut des Sciences du Végétal; Francia
Fil: Ulloa, Rita Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Materia
Arabidopsis Thaliana
Tubulin Phosphorylation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79680
Acceder
id CONICETDig_ee66f38a1d8671e176e01a60c65413a1
oai_identifier_str oai:ri.conicet.gov.ar:11336/79680
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A mutant ankyrin protein kinase from Medicago sativa affects Arabidopsis adventitious rootsChinchilla, DelphineFrugier, FlorianRaices, MarcelaMerchan, FranciscoGiammaria, VeronicaGargantini, Pablo RubenGonzalez Rizzo, SilvinaCrespi, MartinUlloa, Rita MariaArabidopsis ThalianaTubulin Phosphorylationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A family of plant kinases containing ankyrin-repeats, the Ankyrin-Protein Kinases (APKs), shows structural resemblance to mammalian Integrin-Linked Kinases (ILKs), key regulators of mammalian cell adhesion. MsAPK1 expression is induced by osmotic stress in roots of Medicago sativa (L.) plants. The Escherichia coli-purified MsAPK1 could only phosphorylate tubulin among a variety of substrates and the enzymatic activity was strictly dependent on Mn2+. MsAPK1 is highly related to two APK genes in Arabidopsis thaliana (L.), AtAPK1 and AtAPK2. Promoter-GUS fusions assays revealed that the Arabidopsis APK genes show distinct expression patterns in roots and hypocotyls. Although Medicago truncatula (L.) plants affected in MsAPK1 expression could not be obtained using in vitro regeneration, A. thaliana plants expressing MsAPK1 or a mutant MsAPK1 protein, in which the conserved aspartate 315 of the kinase catalytic domain was replaced by asparagines (DN-lines), developed normally. The DN mutant lines showed increased capacity to develop adventitious roots when compared with control or MsAPK1-expressing plants. APK-mediated signalling may therefore link perception of external abiotic signals and the microtubule cytoskeleton, and influence adventitious root development.Fil: Chinchilla, Delphine. Institut des Sciences du Végétal; Francia. Universidad de Basilea; SuizaFil: Frugier, Florian. Institut des Sciences du Végétal; FranciaFil: Raices, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. The Salk Institute for Biological Studies; Estados UnidosFil: Merchan, Francisco. Institut des Sciences du Végétal; FranciaFil: Giammaria, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Gargantini, Pablo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Gonzalez Rizzo, Silvina. Institut des Sciences du Végétal; FranciaFil: Crespi, Martin. Institut des Sciences du Végétal; FranciaFil: Ulloa, Rita Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaCsiro Publishing2008-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79680Chinchilla, Delphine; Frugier, Florian; Raices, Marcela; Merchan, Francisco; Giammaria, Veronica; et al.; A mutant ankyrin protein kinase from Medicago sativa affects Arabidopsis adventitious roots; Csiro Publishing; Functional Plant Biology; 35; 1; 12-2008; 92-1011445-4408CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1071/FP07209info:eu-repo/semantics/altIdentifier/url/http://www.publish.csiro.au/fp/FP07209info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:22:10Zoai:ri.conicet.gov.ar:11336/79680instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:22:10.432CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A mutant ankyrin protein kinase from Medicago sativa affects Arabidopsis adventitious roots
title A mutant ankyrin protein kinase from Medicago sativa affects Arabidopsis adventitious roots
spellingShingle A mutant ankyrin protein kinase from Medicago sativa affects Arabidopsis adventitious roots
Chinchilla, Delphine
Arabidopsis Thaliana
Tubulin Phosphorylation
title_short A mutant ankyrin protein kinase from Medicago sativa affects Arabidopsis adventitious roots
title_full A mutant ankyrin protein kinase from Medicago sativa affects Arabidopsis adventitious roots
title_fullStr A mutant ankyrin protein kinase from Medicago sativa affects Arabidopsis adventitious roots
title_full_unstemmed A mutant ankyrin protein kinase from Medicago sativa affects Arabidopsis adventitious roots
title_sort A mutant ankyrin protein kinase from Medicago sativa affects Arabidopsis adventitious roots
dc.creator.none.fl_str_mv Chinchilla, Delphine
Frugier, Florian
Raices, Marcela
Merchan, Francisco
Giammaria, Veronica
Gargantini, Pablo Ruben
Gonzalez Rizzo, Silvina
Crespi, Martin
Ulloa, Rita Maria
author Chinchilla, Delphine
author_facet Chinchilla, Delphine
Frugier, Florian
Raices, Marcela
Merchan, Francisco
Giammaria, Veronica
Gargantini, Pablo Ruben
Gonzalez Rizzo, Silvina
Crespi, Martin
Ulloa, Rita Maria
author_role author
author2 Frugier, Florian
Raices, Marcela
Merchan, Francisco
Giammaria, Veronica
Gargantini, Pablo Ruben
Gonzalez Rizzo, Silvina
Crespi, Martin
Ulloa, Rita Maria
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Arabidopsis Thaliana
Tubulin Phosphorylation
topic Arabidopsis Thaliana
Tubulin Phosphorylation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A family of plant kinases containing ankyrin-repeats, the Ankyrin-Protein Kinases (APKs), shows structural resemblance to mammalian Integrin-Linked Kinases (ILKs), key regulators of mammalian cell adhesion. MsAPK1 expression is induced by osmotic stress in roots of Medicago sativa (L.) plants. The Escherichia coli-purified MsAPK1 could only phosphorylate tubulin among a variety of substrates and the enzymatic activity was strictly dependent on Mn2+. MsAPK1 is highly related to two APK genes in Arabidopsis thaliana (L.), AtAPK1 and AtAPK2. Promoter-GUS fusions assays revealed that the Arabidopsis APK genes show distinct expression patterns in roots and hypocotyls. Although Medicago truncatula (L.) plants affected in MsAPK1 expression could not be obtained using in vitro regeneration, A. thaliana plants expressing MsAPK1 or a mutant MsAPK1 protein, in which the conserved aspartate 315 of the kinase catalytic domain was replaced by asparagines (DN-lines), developed normally. The DN mutant lines showed increased capacity to develop adventitious roots when compared with control or MsAPK1-expressing plants. APK-mediated signalling may therefore link perception of external abiotic signals and the microtubule cytoskeleton, and influence adventitious root development.
Fil: Chinchilla, Delphine. Institut des Sciences du Végétal; Francia. Universidad de Basilea; Suiza
Fil: Frugier, Florian. Institut des Sciences du Végétal; Francia
Fil: Raices, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. The Salk Institute for Biological Studies; Estados Unidos
Fil: Merchan, Francisco. Institut des Sciences du Végétal; Francia
Fil: Giammaria, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Gargantini, Pablo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Gonzalez Rizzo, Silvina. Institut des Sciences du Végétal; Francia
Fil: Crespi, Martin. Institut des Sciences du Végétal; Francia
Fil: Ulloa, Rita Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
description A family of plant kinases containing ankyrin-repeats, the Ankyrin-Protein Kinases (APKs), shows structural resemblance to mammalian Integrin-Linked Kinases (ILKs), key regulators of mammalian cell adhesion. MsAPK1 expression is induced by osmotic stress in roots of Medicago sativa (L.) plants. The Escherichia coli-purified MsAPK1 could only phosphorylate tubulin among a variety of substrates and the enzymatic activity was strictly dependent on Mn2+. MsAPK1 is highly related to two APK genes in Arabidopsis thaliana (L.), AtAPK1 and AtAPK2. Promoter-GUS fusions assays revealed that the Arabidopsis APK genes show distinct expression patterns in roots and hypocotyls. Although Medicago truncatula (L.) plants affected in MsAPK1 expression could not be obtained using in vitro regeneration, A. thaliana plants expressing MsAPK1 or a mutant MsAPK1 protein, in which the conserved aspartate 315 of the kinase catalytic domain was replaced by asparagines (DN-lines), developed normally. The DN mutant lines showed increased capacity to develop adventitious roots when compared with control or MsAPK1-expressing plants. APK-mediated signalling may therefore link perception of external abiotic signals and the microtubule cytoskeleton, and influence adventitious root development.
publishDate 2008
dc.date.none.fl_str_mv 2008-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79680
Chinchilla, Delphine; Frugier, Florian; Raices, Marcela; Merchan, Francisco; Giammaria, Veronica; et al.; A mutant ankyrin protein kinase from Medicago sativa affects Arabidopsis adventitious roots; Csiro Publishing; Functional Plant Biology; 35; 1; 12-2008; 92-101
1445-4408
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79680
identifier_str_mv Chinchilla, Delphine; Frugier, Florian; Raices, Marcela; Merchan, Francisco; Giammaria, Veronica; et al.; A mutant ankyrin protein kinase from Medicago sativa affects Arabidopsis adventitious roots; Csiro Publishing; Functional Plant Biology; 35; 1; 12-2008; 92-101
1445-4408
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1071/FP07209
info:eu-repo/semantics/altIdentifier/url/http://www.publish.csiro.au/fp/FP07209
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Csiro Publishing
publisher.none.fl_str_mv Csiro Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981220723458048
score 12.48226

Publicaciones similares