KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1

Autores
Karlsson, Patricia; Christie, Michael Danger; Seymour, Danelle K.; Wang, Huan; Wang, Xi; Hagmann, Jörg; Kulcheski, Franceli; Manavella, Pablo Andrés
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The biogenesis of microRNAs (miRNAs), which regulate mRNA abundance through posttranscriptional silencing, comprises multiple well-orchestrated processing steps. We have identified the Arabidopsis thaliana K homology (KH) domain protein REGULATOR OF CBF GENE EXPRESSION 3 (RCF3) as a cofactor affecting miRNA biogenesis in specific plant tissues. MiRNA and miRNA-target levels were reduced in apex-enriched samples of rcf3 mutants, but not in other tissues. Mechanistically, RCF3 affects miRNA biogenesis through nuclear interactions with the phosphatases C-TERMINAL DOMAIN PHOSPHATASE-LIKE1 and 2 (CPL1 and CPL2). These interactions are essential to regulate the phosphorylation status, and thus the activity, of the double-stranded RNA binding protein and DICER-LIKE1 (DCL1) cofactor HYPONASTIC LEAVES1 (HYL1).
Fil: Karlsson, Patricia. Max Planck Institute for Developmental Biology; Alemania
Fil: Christie, Michael Danger. Max Planck Institute for Developmental Biology; Alemania
Fil: Seymour, Danelle K.. Max Planck Institute for Developmental Biology; Alemania
Fil: Wang, Huan. Max Planck Institute for Developmental Biology; Alemania
Fil: Wang, Xi. Max Planck Institute for Developmental Biology; Alemania
Fil: Hagmann, Jörg. Max Planck Institute for Developmental Biology; Alemania
Fil: Kulcheski, Franceli. Max Planck Institute for Developmental Biology; Alemania
Fil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Materia
Arabidopsis Thaliana
Hyl1
Gene Silencing
Micro Rna Biogenesis
Phosphorylation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/40398
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/40398
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1Karlsson, PatriciaChristie, Michael DangerSeymour, Danelle K.Wang, HuanWang, XiHagmann, JörgKulcheski, FranceliManavella, Pablo AndrésArabidopsis ThalianaHyl1Gene SilencingMicro Rna BiogenesisPhosphorylationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The biogenesis of microRNAs (miRNAs), which regulate mRNA abundance through posttranscriptional silencing, comprises multiple well-orchestrated processing steps. We have identified the Arabidopsis thaliana K homology (KH) domain protein REGULATOR OF CBF GENE EXPRESSION 3 (RCF3) as a cofactor affecting miRNA biogenesis in specific plant tissues. MiRNA and miRNA-target levels were reduced in apex-enriched samples of rcf3 mutants, but not in other tissues. Mechanistically, RCF3 affects miRNA biogenesis through nuclear interactions with the phosphatases C-TERMINAL DOMAIN PHOSPHATASE-LIKE1 and 2 (CPL1 and CPL2). These interactions are essential to regulate the phosphorylation status, and thus the activity, of the double-stranded RNA binding protein and DICER-LIKE1 (DCL1) cofactor HYPONASTIC LEAVES1 (HYL1).Fil: Karlsson, Patricia. Max Planck Institute for Developmental Biology; AlemaniaFil: Christie, Michael Danger. Max Planck Institute for Developmental Biology; AlemaniaFil: Seymour, Danelle K.. Max Planck Institute for Developmental Biology; AlemaniaFil: Wang, Huan. Max Planck Institute for Developmental Biology; AlemaniaFil: Wang, Xi. Max Planck Institute for Developmental Biology; AlemaniaFil: Hagmann, Jörg. Max Planck Institute for Developmental Biology; AlemaniaFil: Kulcheski, Franceli. Max Planck Institute for Developmental Biology; AlemaniaFil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaNational Academy of Sciences2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40398Karlsson, Patricia; Christie, Michael Danger; Seymour, Danelle K.; Wang, Huan; Wang, Xi; et al.; KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 112; 45; 11-2015; 14096-141010027-8424CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.1512865112info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:19:19Zoai:ri.conicet.gov.ar:11336/40398instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:19:19.302CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1
title KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1
spellingShingle KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1
Karlsson, Patricia
Arabidopsis Thaliana
Hyl1
Gene Silencing
Micro Rna Biogenesis
Phosphorylation
title_short KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1
title_full KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1
title_fullStr KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1
title_full_unstemmed KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1
title_sort KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1
dc.creator.none.fl_str_mv Karlsson, Patricia
Christie, Michael Danger
Seymour, Danelle K.
Wang, Huan
Wang, Xi
Hagmann, Jörg
Kulcheski, Franceli
Manavella, Pablo Andrés
author Karlsson, Patricia
author_facet Karlsson, Patricia
Christie, Michael Danger
Seymour, Danelle K.
Wang, Huan
Wang, Xi
Hagmann, Jörg
Kulcheski, Franceli
Manavella, Pablo Andrés
author_role author
author2 Christie, Michael Danger
Seymour, Danelle K.
Wang, Huan
Wang, Xi
Hagmann, Jörg
Kulcheski, Franceli
Manavella, Pablo Andrés
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Arabidopsis Thaliana
Hyl1
Gene Silencing
Micro Rna Biogenesis
Phosphorylation
topic Arabidopsis Thaliana
Hyl1
Gene Silencing
Micro Rna Biogenesis
Phosphorylation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The biogenesis of microRNAs (miRNAs), which regulate mRNA abundance through posttranscriptional silencing, comprises multiple well-orchestrated processing steps. We have identified the Arabidopsis thaliana K homology (KH) domain protein REGULATOR OF CBF GENE EXPRESSION 3 (RCF3) as a cofactor affecting miRNA biogenesis in specific plant tissues. MiRNA and miRNA-target levels were reduced in apex-enriched samples of rcf3 mutants, but not in other tissues. Mechanistically, RCF3 affects miRNA biogenesis through nuclear interactions with the phosphatases C-TERMINAL DOMAIN PHOSPHATASE-LIKE1 and 2 (CPL1 and CPL2). These interactions are essential to regulate the phosphorylation status, and thus the activity, of the double-stranded RNA binding protein and DICER-LIKE1 (DCL1) cofactor HYPONASTIC LEAVES1 (HYL1).
Fil: Karlsson, Patricia. Max Planck Institute for Developmental Biology; Alemania
Fil: Christie, Michael Danger. Max Planck Institute for Developmental Biology; Alemania
Fil: Seymour, Danelle K.. Max Planck Institute for Developmental Biology; Alemania
Fil: Wang, Huan. Max Planck Institute for Developmental Biology; Alemania
Fil: Wang, Xi. Max Planck Institute for Developmental Biology; Alemania
Fil: Hagmann, Jörg. Max Planck Institute for Developmental Biology; Alemania
Fil: Kulcheski, Franceli. Max Planck Institute for Developmental Biology; Alemania
Fil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
description The biogenesis of microRNAs (miRNAs), which regulate mRNA abundance through posttranscriptional silencing, comprises multiple well-orchestrated processing steps. We have identified the Arabidopsis thaliana K homology (KH) domain protein REGULATOR OF CBF GENE EXPRESSION 3 (RCF3) as a cofactor affecting miRNA biogenesis in specific plant tissues. MiRNA and miRNA-target levels were reduced in apex-enriched samples of rcf3 mutants, but not in other tissues. Mechanistically, RCF3 affects miRNA biogenesis through nuclear interactions with the phosphatases C-TERMINAL DOMAIN PHOSPHATASE-LIKE1 and 2 (CPL1 and CPL2). These interactions are essential to regulate the phosphorylation status, and thus the activity, of the double-stranded RNA binding protein and DICER-LIKE1 (DCL1) cofactor HYPONASTIC LEAVES1 (HYL1).
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/40398
Karlsson, Patricia; Christie, Michael Danger; Seymour, Danelle K.; Wang, Huan; Wang, Xi; et al.; KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 112; 45; 11-2015; 14096-14101
0027-8424
CONICET Digital
CONICET
url http://hdl.handle.net/11336/40398
identifier_str_mv Karlsson, Patricia; Christie, Michael Danger; Seymour, Danelle K.; Wang, Huan; Wang, Xi; et al.; KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 112; 45; 11-2015; 14096-14101
0027-8424
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.1512865112
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614163958071297
score 13.070432

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