Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection

Autores
Masachessi, Gisela; Pisano, María Belén; Prez, Verónica Emilse; Martínez, L.C.; Michelena, J. F.; Martinez Wassaf, Maribel Graciela; Giordano, Miguel Oscar; Isa, Maria Beatriz; Pavan, Jorge Victorio; Welter, Adriana Beatriz; Nates, Silvia Viviana; Ré, Viviana Elizabeth
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Water resources contaminated with wastewater are an important source for the dissemination of enteric viruses with an impact on the health of the population. The aim of the study was to assess the viral contamination of freshwater from a dam in Argentina by using infectious enterovirus detection, viral RNA amplification, and a genetic characterization of five enteric viruses associated with diarrhea and hepatitis. Enterovirus infectivity (iEV) was evaluated by cell culture and direct immunofluorescence. The detection of the viral genome of rotavirus (RV), human astrovirus (HAstV), norovirus (NoV), hepatitis A virus (HAV), and hepatitis E virus (HEV) was performed by reverse transcriptase PCR (RT-PCR). A total of 48 water samples from 4 monitoring points on the body of the dam from January to December 2012 and 66 water samples from 3 tourist beaches on the edge of the dam from October 2013 to October 2015 were collected monthly. During the first period, the overall viral frequency detection was 52.1% for group A RV, 50% for HAstV, 60.4% for NoV, 22.9% for HAV, 2.1% for HEV, and 64.6% for iEV. The overall frequency detection for the second sampling was 18.2% for RV and HAstV, 31.8% for NoV, 7.57% for HEV, and 66.7% for iEV. There was no detection of HAV during this period. The genotypes and genogroups detected through the study correlated with the most common genomic variants associated with human gastrointestinal and hepatitis illnesses. The results obtained could alert the health systems and environmental sanitation to make decisions for viral control and prevention in our environment.
Fil: Masachessi, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Fil: Pisano, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Fil: Prez, Verónica Emilse. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Fil: Martínez, L.C.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Fil: Michelena, J. F.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Fil: Martinez Wassaf, Maribel Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Giordano, Miguel Oscar. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Fil: Isa, Maria Beatriz. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Fil: Pavan, Jorge Victorio. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Fil: Welter, Adriana Beatriz. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Nates, Silvia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Fil: Ré, Viviana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Materia
ENTERIC VIRUSES
FRESHWATER VIRAL CONTAMINATION
GASTROINTESTINAL VIRUSES
GENOTYPE/GENOGROUP VARIANTS
HEPATITIS VIRUSES
IMMUNOFLUORESCENCE
RT-PCR
VIABLE ENTEROVIRUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66298
Acceder
id CONICETDig_ee371338ef5cb99853d11540cf1b5a9f
oai_identifier_str oai:ri.conicet.gov.ar:11336/66298
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus DetectionMasachessi, GiselaPisano, María BelénPrez, Verónica EmilseMartínez, L.C.Michelena, J. F.Martinez Wassaf, Maribel GracielaGiordano, Miguel OscarIsa, Maria BeatrizPavan, Jorge VictorioWelter, Adriana BeatrizNates, Silvia VivianaRé, Viviana ElizabethENTERIC VIRUSESFRESHWATER VIRAL CONTAMINATIONGASTROINTESTINAL VIRUSESGENOTYPE/GENOGROUP VARIANTSHEPATITIS VIRUSESIMMUNOFLUORESCENCERT-PCRVIABLE ENTEROVIRUShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Water resources contaminated with wastewater are an important source for the dissemination of enteric viruses with an impact on the health of the population. The aim of the study was to assess the viral contamination of freshwater from a dam in Argentina by using infectious enterovirus detection, viral RNA amplification, and a genetic characterization of five enteric viruses associated with diarrhea and hepatitis. Enterovirus infectivity (iEV) was evaluated by cell culture and direct immunofluorescence. The detection of the viral genome of rotavirus (RV), human astrovirus (HAstV), norovirus (NoV), hepatitis A virus (HAV), and hepatitis E virus (HEV) was performed by reverse transcriptase PCR (RT-PCR). A total of 48 water samples from 4 monitoring points on the body of the dam from January to December 2012 and 66 water samples from 3 tourist beaches on the edge of the dam from October 2013 to October 2015 were collected monthly. During the first period, the overall viral frequency detection was 52.1% for group A RV, 50% for HAstV, 60.4% for NoV, 22.9% for HAV, 2.1% for HEV, and 64.6% for iEV. The overall frequency detection for the second sampling was 18.2% for RV and HAstV, 31.8% for NoV, 7.57% for HEV, and 66.7% for iEV. There was no detection of HAV during this period. The genotypes and genogroups detected through the study correlated with the most common genomic variants associated with human gastrointestinal and hepatitis illnesses. The results obtained could alert the health systems and environmental sanitation to make decisions for viral control and prevention in our environment.Fil: Masachessi, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; ArgentinaFil: Pisano, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; ArgentinaFil: Prez, Verónica Emilse. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; ArgentinaFil: Martínez, L.C.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; ArgentinaFil: Michelena, J. F.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; ArgentinaFil: Martinez Wassaf, Maribel Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Giordano, Miguel Oscar. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; ArgentinaFil: Isa, Maria Beatriz. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; ArgentinaFil: Pavan, Jorge Victorio. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; ArgentinaFil: Welter, Adriana Beatriz. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Nates, Silvia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; ArgentinaFil: Ré, Viviana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; ArgentinaAmerican Society for Microbiology2018-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66298Masachessi, Gisela; Pisano, María Belén; Prez, Verónica Emilse; Martínez, L.C.; Michelena, J. F.; et al.; Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection; American Society for Microbiology; Applied And Environmental Microbiology; 84; 5; 2-2018; 1-120099-2240CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.02327-17info:eu-repo/semantics/altIdentifier/url/https://aem.asm.org/content/84/5/e02327-17info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:43Zoai:ri.conicet.gov.ar:11336/66298instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:44.057CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection
title Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection
spellingShingle Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection
Masachessi, Gisela
ENTERIC VIRUSES
FRESHWATER VIRAL CONTAMINATION
GASTROINTESTINAL VIRUSES
GENOTYPE/GENOGROUP VARIANTS
HEPATITIS VIRUSES
IMMUNOFLUORESCENCE
RT-PCR
VIABLE ENTEROVIRUS
title_short Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection
title_full Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection
title_fullStr Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection
title_full_unstemmed Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection
title_sort Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection
dc.creator.none.fl_str_mv Masachessi, Gisela
Pisano, María Belén
Prez, Verónica Emilse
Martínez, L.C.
Michelena, J. F.
Martinez Wassaf, Maribel Graciela
Giordano, Miguel Oscar
Isa, Maria Beatriz
Pavan, Jorge Victorio
Welter, Adriana Beatriz
Nates, Silvia Viviana
Ré, Viviana Elizabeth
author Masachessi, Gisela
author_facet Masachessi, Gisela
Pisano, María Belén
Prez, Verónica Emilse
Martínez, L.C.
Michelena, J. F.
Martinez Wassaf, Maribel Graciela
Giordano, Miguel Oscar
Isa, Maria Beatriz
Pavan, Jorge Victorio
Welter, Adriana Beatriz
Nates, Silvia Viviana
Ré, Viviana Elizabeth
author_role author
author2 Pisano, María Belén
Prez, Verónica Emilse
Martínez, L.C.
Michelena, J. F.
Martinez Wassaf, Maribel Graciela
Giordano, Miguel Oscar
Isa, Maria Beatriz
Pavan, Jorge Victorio
Welter, Adriana Beatriz
Nates, Silvia Viviana
Ré, Viviana Elizabeth
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ENTERIC VIRUSES
FRESHWATER VIRAL CONTAMINATION
GASTROINTESTINAL VIRUSES
GENOTYPE/GENOGROUP VARIANTS
HEPATITIS VIRUSES
IMMUNOFLUORESCENCE
RT-PCR
VIABLE ENTEROVIRUS
topic ENTERIC VIRUSES
FRESHWATER VIRAL CONTAMINATION
GASTROINTESTINAL VIRUSES
GENOTYPE/GENOGROUP VARIANTS
HEPATITIS VIRUSES
IMMUNOFLUORESCENCE
RT-PCR
VIABLE ENTEROVIRUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Water resources contaminated with wastewater are an important source for the dissemination of enteric viruses with an impact on the health of the population. The aim of the study was to assess the viral contamination of freshwater from a dam in Argentina by using infectious enterovirus detection, viral RNA amplification, and a genetic characterization of five enteric viruses associated with diarrhea and hepatitis. Enterovirus infectivity (iEV) was evaluated by cell culture and direct immunofluorescence. The detection of the viral genome of rotavirus (RV), human astrovirus (HAstV), norovirus (NoV), hepatitis A virus (HAV), and hepatitis E virus (HEV) was performed by reverse transcriptase PCR (RT-PCR). A total of 48 water samples from 4 monitoring points on the body of the dam from January to December 2012 and 66 water samples from 3 tourist beaches on the edge of the dam from October 2013 to October 2015 were collected monthly. During the first period, the overall viral frequency detection was 52.1% for group A RV, 50% for HAstV, 60.4% for NoV, 22.9% for HAV, 2.1% for HEV, and 64.6% for iEV. The overall frequency detection for the second sampling was 18.2% for RV and HAstV, 31.8% for NoV, 7.57% for HEV, and 66.7% for iEV. There was no detection of HAV during this period. The genotypes and genogroups detected through the study correlated with the most common genomic variants associated with human gastrointestinal and hepatitis illnesses. The results obtained could alert the health systems and environmental sanitation to make decisions for viral control and prevention in our environment.
Fil: Masachessi, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Fil: Pisano, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Fil: Prez, Verónica Emilse. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Fil: Martínez, L.C.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Fil: Michelena, J. F.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Fil: Martinez Wassaf, Maribel Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Giordano, Miguel Oscar. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Fil: Isa, Maria Beatriz. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Fil: Pavan, Jorge Victorio. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Fil: Welter, Adriana Beatriz. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Nates, Silvia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Fil: Ré, Viviana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
description Water resources contaminated with wastewater are an important source for the dissemination of enteric viruses with an impact on the health of the population. The aim of the study was to assess the viral contamination of freshwater from a dam in Argentina by using infectious enterovirus detection, viral RNA amplification, and a genetic characterization of five enteric viruses associated with diarrhea and hepatitis. Enterovirus infectivity (iEV) was evaluated by cell culture and direct immunofluorescence. The detection of the viral genome of rotavirus (RV), human astrovirus (HAstV), norovirus (NoV), hepatitis A virus (HAV), and hepatitis E virus (HEV) was performed by reverse transcriptase PCR (RT-PCR). A total of 48 water samples from 4 monitoring points on the body of the dam from January to December 2012 and 66 water samples from 3 tourist beaches on the edge of the dam from October 2013 to October 2015 were collected monthly. During the first period, the overall viral frequency detection was 52.1% for group A RV, 50% for HAstV, 60.4% for NoV, 22.9% for HAV, 2.1% for HEV, and 64.6% for iEV. The overall frequency detection for the second sampling was 18.2% for RV and HAstV, 31.8% for NoV, 7.57% for HEV, and 66.7% for iEV. There was no detection of HAV during this period. The genotypes and genogroups detected through the study correlated with the most common genomic variants associated with human gastrointestinal and hepatitis illnesses. The results obtained could alert the health systems and environmental sanitation to make decisions for viral control and prevention in our environment.
publishDate 2018
dc.date.none.fl_str_mv 2018-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66298
Masachessi, Gisela; Pisano, María Belén; Prez, Verónica Emilse; Martínez, L.C.; Michelena, J. F.; et al.; Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection; American Society for Microbiology; Applied And Environmental Microbiology; 84; 5; 2-2018; 1-12
0099-2240
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66298
identifier_str_mv Masachessi, Gisela; Pisano, María Belén; Prez, Verónica Emilse; Martínez, L.C.; Michelena, J. F.; et al.; Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection; American Society for Microbiology; Applied And Environmental Microbiology; 84; 5; 2-2018; 1-12
0099-2240
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.02327-17
info:eu-repo/semantics/altIdentifier/url/https://aem.asm.org/content/84/5/e02327-17
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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