Specific primer sets used to amplify by PCR the hepatitis B virus overlapping S/Pol region select different viral variants

Autores
Cuestas, María Luján; Mathet, Veronica Lidia; Oubiña, Jose Raul
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
PCR detection of viral genomes has provided new insights into viral diagnosis. Nowadays, it is the most frequently used nucleic acid testing (qualitative and quantitative) technique. The aim of this study was to analyse the major circulating hepatitis B virus (HBV) variants PCR-amplified by three sets of primers in a patient infected with genotype E. The HBV S/Pol overlapping genomic region was amplified from the serum of an infected child using three primer sets previously described. Sequence analysis corresponding to the HBV S/Pol region revealed the presence of different viral populations depending on the set of primers used. D144A S-escape mutant was detected with two of the primer sets, while the rtL217R mutant within the Pol - conferring resistance to Adefovir - could be picked up with a different pair of primer sets. This study undoubtedly implies that the description of viral polymorphisms should be stated together with the sequence of the primers used for PCR amplification when studies of escape and/or antiviral-resistant HBV mutants are carried out.
Fil: Cuestas, María Luján. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Area Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mathet, Veronica Lidia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Area Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Oubiña, Jose Raul. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Area Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Hepatitis B Virus
Primer Sets
Variants
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67305
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Specific primer sets used to amplify by PCR the hepatitis B virus overlapping S/Pol region select different viral variantsCuestas, María LujánMathet, Veronica LidiaOubiña, Jose RaulHepatitis B VirusPrimer SetsVariantshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3PCR detection of viral genomes has provided new insights into viral diagnosis. Nowadays, it is the most frequently used nucleic acid testing (qualitative and quantitative) technique. The aim of this study was to analyse the major circulating hepatitis B virus (HBV) variants PCR-amplified by three sets of primers in a patient infected with genotype E. The HBV S/Pol overlapping genomic region was amplified from the serum of an infected child using three primer sets previously described. Sequence analysis corresponding to the HBV S/Pol region revealed the presence of different viral populations depending on the set of primers used. D144A S-escape mutant was detected with two of the primer sets, while the rtL217R mutant within the Pol - conferring resistance to Adefovir - could be picked up with a different pair of primer sets. This study undoubtedly implies that the description of viral polymorphisms should be stated together with the sequence of the primers used for PCR amplification when studies of escape and/or antiviral-resistant HBV mutants are carried out.Fil: Cuestas, María Luján. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Area Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mathet, Veronica Lidia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Area Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Oubiña, Jose Raul. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Area Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley Blackwell Publishing, Inc2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67305Cuestas, María Luján; Mathet, Veronica Lidia; Oubiña, Jose Raul; Specific primer sets used to amplify by PCR the hepatitis B virus overlapping S/Pol region select different viral variants; Wiley Blackwell Publishing, Inc; Journal Of Viral Hepatitis.; 19; 10; 10-2012; 754-7561352-0504CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2893.2012.01614.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2893.2012.01614.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:14Zoai:ri.conicet.gov.ar:11336/67305instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:14.958CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Specific primer sets used to amplify by PCR the hepatitis B virus overlapping S/Pol region select different viral variants
title Specific primer sets used to amplify by PCR the hepatitis B virus overlapping S/Pol region select different viral variants
spellingShingle Specific primer sets used to amplify by PCR the hepatitis B virus overlapping S/Pol region select different viral variants
Cuestas, María Luján
Hepatitis B Virus
Primer Sets
Variants
title_short Specific primer sets used to amplify by PCR the hepatitis B virus overlapping S/Pol region select different viral variants
title_full Specific primer sets used to amplify by PCR the hepatitis B virus overlapping S/Pol region select different viral variants
title_fullStr Specific primer sets used to amplify by PCR the hepatitis B virus overlapping S/Pol region select different viral variants
title_full_unstemmed Specific primer sets used to amplify by PCR the hepatitis B virus overlapping S/Pol region select different viral variants
title_sort Specific primer sets used to amplify by PCR the hepatitis B virus overlapping S/Pol region select different viral variants
dc.creator.none.fl_str_mv Cuestas, María Luján
Mathet, Veronica Lidia
Oubiña, Jose Raul
author Cuestas, María Luján
author_facet Cuestas, María Luján
Mathet, Veronica Lidia
Oubiña, Jose Raul
author_role author
author2 Mathet, Veronica Lidia
Oubiña, Jose Raul
author2_role author
author
dc.subject.none.fl_str_mv Hepatitis B Virus
Primer Sets
Variants
topic Hepatitis B Virus
Primer Sets
Variants
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv PCR detection of viral genomes has provided new insights into viral diagnosis. Nowadays, it is the most frequently used nucleic acid testing (qualitative and quantitative) technique. The aim of this study was to analyse the major circulating hepatitis B virus (HBV) variants PCR-amplified by three sets of primers in a patient infected with genotype E. The HBV S/Pol overlapping genomic region was amplified from the serum of an infected child using three primer sets previously described. Sequence analysis corresponding to the HBV S/Pol region revealed the presence of different viral populations depending on the set of primers used. D144A S-escape mutant was detected with two of the primer sets, while the rtL217R mutant within the Pol - conferring resistance to Adefovir - could be picked up with a different pair of primer sets. This study undoubtedly implies that the description of viral polymorphisms should be stated together with the sequence of the primers used for PCR amplification when studies of escape and/or antiviral-resistant HBV mutants are carried out.
Fil: Cuestas, María Luján. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Area Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mathet, Veronica Lidia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Area Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Oubiña, Jose Raul. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Area Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description PCR detection of viral genomes has provided new insights into viral diagnosis. Nowadays, it is the most frequently used nucleic acid testing (qualitative and quantitative) technique. The aim of this study was to analyse the major circulating hepatitis B virus (HBV) variants PCR-amplified by three sets of primers in a patient infected with genotype E. The HBV S/Pol overlapping genomic region was amplified from the serum of an infected child using three primer sets previously described. Sequence analysis corresponding to the HBV S/Pol region revealed the presence of different viral populations depending on the set of primers used. D144A S-escape mutant was detected with two of the primer sets, while the rtL217R mutant within the Pol - conferring resistance to Adefovir - could be picked up with a different pair of primer sets. This study undoubtedly implies that the description of viral polymorphisms should be stated together with the sequence of the primers used for PCR amplification when studies of escape and/or antiviral-resistant HBV mutants are carried out.
publishDate 2012
dc.date.none.fl_str_mv 2012-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67305
Cuestas, María Luján; Mathet, Veronica Lidia; Oubiña, Jose Raul; Specific primer sets used to amplify by PCR the hepatitis B virus overlapping S/Pol region select different viral variants; Wiley Blackwell Publishing, Inc; Journal Of Viral Hepatitis.; 19; 10; 10-2012; 754-756
1352-0504
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67305
identifier_str_mv Cuestas, María Luján; Mathet, Veronica Lidia; Oubiña, Jose Raul; Specific primer sets used to amplify by PCR the hepatitis B virus overlapping S/Pol region select different viral variants; Wiley Blackwell Publishing, Inc; Journal Of Viral Hepatitis.; 19; 10; 10-2012; 754-756
1352-0504
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2893.2012.01614.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2893.2012.01614.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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