Is the prominent ericoid mycorrhizal fungus Rhizoscyphus ericae absent in the Southern Hemisphere’s Ericaceae? A case study on the diversity of root mycobionts in Gaultheria spp. f...

Autores
Bruzone, María Clara; Fontenla, Sonia; Vohnik, Martin
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ericaceae diversity hotspots are in the mountains of the Neotropics and Papua New Guinea, South Africa’s fynbos and Southeast Asia but majority of references to their root mycobionts come from the Northern Hemisphere. Here, typical cultivable ericoid mycorrhizal (ErM) fungi comprise Rhizoscyphus ericae, Meliniomyces variabilis, and Oidiodendron maius. It is however unclear whether this is true also for the Southern Hemisphere. Our study focused on cultivable mycobionts from hair roots of Gaultheria mucronata and Gaultheria poeppigii (Ericaceae) from two natural forests in NW Patagonia, Argentina, differing in mycorrhizal preferences of their tree dominants. We detected 62 well-defined OTUs mostly belonging to Helotiales and Hypocreales; the most frequent were Phialocephala fortinii s. l., Pochonia suchlasporia, and Ilyonectria radicicola. Only one out of 257 isolates showed ITS nrDNA similarity to members of the R. ericae aggregate (REA) but was not conspecific with R. ericae, and only five isolates were conspecific with O. maius. Microscopic observations showed that the screened roots were frequently colonized in a manner differing from the pattern typically produced by R. ericae and O. maius. A re-synthesis experiment with selected isolates showed that only O. maius formed colonization resembling ericoid mycorrhiza. Amplification of root fungal DNA with REA-specific and Sebacinaceae-specific primers showed that REA mycobionts were present in some of the screened samples while Sebacinaceae were present in all samples. These results suggest that Gaultheria spp. from NW Patagonia form ericoid mycorrhizae predominantly with the difficult-to-cultivate Sebacinaceae while the incidence of REA is relatively low and may be masked by other most likely non-mycorrhizal cultivable mycobionts.
Fil: Bruzone, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Fontenla, Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Vohnik, Martin. Charles University; República Checa
Materia
Ericoid Mycorrhiza
Root Endophyte
Phialocephala Fortinii
Sebacinaceae
Rhizoscyphus Ericae Ericae Specific Primers
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32692
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Is the prominent ericoid mycorrhizal fungus Rhizoscyphus ericae absent in the Southern Hemisphere’s Ericaceae? A case study on the diversity of root mycobionts in Gaultheria spp. from northwest Patagonia, ArgentinaBruzone, María ClaraFontenla, SoniaVohnik, MartinEricoid MycorrhizaRoot EndophytePhialocephala FortiniiSebacinaceaeRhizoscyphus Ericae Ericae Specific Primershttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Ericaceae diversity hotspots are in the mountains of the Neotropics and Papua New Guinea, South Africa’s fynbos and Southeast Asia but majority of references to their root mycobionts come from the Northern Hemisphere. Here, typical cultivable ericoid mycorrhizal (ErM) fungi comprise Rhizoscyphus ericae, Meliniomyces variabilis, and Oidiodendron maius. It is however unclear whether this is true also for the Southern Hemisphere. Our study focused on cultivable mycobionts from hair roots of Gaultheria mucronata and Gaultheria poeppigii (Ericaceae) from two natural forests in NW Patagonia, Argentina, differing in mycorrhizal preferences of their tree dominants. We detected 62 well-defined OTUs mostly belonging to Helotiales and Hypocreales; the most frequent were Phialocephala fortinii s. l., Pochonia suchlasporia, and Ilyonectria radicicola. Only one out of 257 isolates showed ITS nrDNA similarity to members of the R. ericae aggregate (REA) but was not conspecific with R. ericae, and only five isolates were conspecific with O. maius. Microscopic observations showed that the screened roots were frequently colonized in a manner differing from the pattern typically produced by R. ericae and O. maius. A re-synthesis experiment with selected isolates showed that only O. maius formed colonization resembling ericoid mycorrhiza. Amplification of root fungal DNA with REA-specific and Sebacinaceae-specific primers showed that REA mycobionts were present in some of the screened samples while Sebacinaceae were present in all samples. These results suggest that Gaultheria spp. from NW Patagonia form ericoid mycorrhizae predominantly with the difficult-to-cultivate Sebacinaceae while the incidence of REA is relatively low and may be masked by other most likely non-mycorrhizal cultivable mycobionts.Fil: Bruzone, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Fontenla, Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Vohnik, Martin. Charles University; República ChecaSpringer2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32692Vohnik, Martin; Fontenla, Sonia; Bruzone, María Clara; Is the prominent ericoid mycorrhizal fungus Rhizoscyphus ericae absent in the Southern Hemisphere’s Ericaceae? A case study on the diversity of root mycobionts in Gaultheria spp. from northwest Patagonia, Argentina; Springer; Mycorrhiza; 25; 1; 5-2014; 25-400940-63601432-1890CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00572-014-0586-3info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00572-014-0586-3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:17:00Zoai:ri.conicet.gov.ar:11336/32692instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:17:01.117CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Is the prominent ericoid mycorrhizal fungus Rhizoscyphus ericae absent in the Southern Hemisphere’s Ericaceae? A case study on the diversity of root mycobionts in Gaultheria spp. from northwest Patagonia, Argentina
title Is the prominent ericoid mycorrhizal fungus Rhizoscyphus ericae absent in the Southern Hemisphere’s Ericaceae? A case study on the diversity of root mycobionts in Gaultheria spp. from northwest Patagonia, Argentina
spellingShingle Is the prominent ericoid mycorrhizal fungus Rhizoscyphus ericae absent in the Southern Hemisphere’s Ericaceae? A case study on the diversity of root mycobionts in Gaultheria spp. from northwest Patagonia, Argentina
Bruzone, María Clara
Ericoid Mycorrhiza
Root Endophyte
Phialocephala Fortinii
Sebacinaceae
Rhizoscyphus Ericae Ericae Specific Primers
title_short Is the prominent ericoid mycorrhizal fungus Rhizoscyphus ericae absent in the Southern Hemisphere’s Ericaceae? A case study on the diversity of root mycobionts in Gaultheria spp. from northwest Patagonia, Argentina
title_full Is the prominent ericoid mycorrhizal fungus Rhizoscyphus ericae absent in the Southern Hemisphere’s Ericaceae? A case study on the diversity of root mycobionts in Gaultheria spp. from northwest Patagonia, Argentina
title_fullStr Is the prominent ericoid mycorrhizal fungus Rhizoscyphus ericae absent in the Southern Hemisphere’s Ericaceae? A case study on the diversity of root mycobionts in Gaultheria spp. from northwest Patagonia, Argentina
title_full_unstemmed Is the prominent ericoid mycorrhizal fungus Rhizoscyphus ericae absent in the Southern Hemisphere’s Ericaceae? A case study on the diversity of root mycobionts in Gaultheria spp. from northwest Patagonia, Argentina
title_sort Is the prominent ericoid mycorrhizal fungus Rhizoscyphus ericae absent in the Southern Hemisphere’s Ericaceae? A case study on the diversity of root mycobionts in Gaultheria spp. from northwest Patagonia, Argentina
dc.creator.none.fl_str_mv Bruzone, María Clara
Fontenla, Sonia
Vohnik, Martin
author Bruzone, María Clara
author_facet Bruzone, María Clara
Fontenla, Sonia
Vohnik, Martin
author_role author
author2 Fontenla, Sonia
Vohnik, Martin
author2_role author
author
dc.subject.none.fl_str_mv Ericoid Mycorrhiza
Root Endophyte
Phialocephala Fortinii
Sebacinaceae
Rhizoscyphus Ericae Ericae Specific Primers
topic Ericoid Mycorrhiza
Root Endophyte
Phialocephala Fortinii
Sebacinaceae
Rhizoscyphus Ericae Ericae Specific Primers
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Ericaceae diversity hotspots are in the mountains of the Neotropics and Papua New Guinea, South Africa’s fynbos and Southeast Asia but majority of references to their root mycobionts come from the Northern Hemisphere. Here, typical cultivable ericoid mycorrhizal (ErM) fungi comprise Rhizoscyphus ericae, Meliniomyces variabilis, and Oidiodendron maius. It is however unclear whether this is true also for the Southern Hemisphere. Our study focused on cultivable mycobionts from hair roots of Gaultheria mucronata and Gaultheria poeppigii (Ericaceae) from two natural forests in NW Patagonia, Argentina, differing in mycorrhizal preferences of their tree dominants. We detected 62 well-defined OTUs mostly belonging to Helotiales and Hypocreales; the most frequent were Phialocephala fortinii s. l., Pochonia suchlasporia, and Ilyonectria radicicola. Only one out of 257 isolates showed ITS nrDNA similarity to members of the R. ericae aggregate (REA) but was not conspecific with R. ericae, and only five isolates were conspecific with O. maius. Microscopic observations showed that the screened roots were frequently colonized in a manner differing from the pattern typically produced by R. ericae and O. maius. A re-synthesis experiment with selected isolates showed that only O. maius formed colonization resembling ericoid mycorrhiza. Amplification of root fungal DNA with REA-specific and Sebacinaceae-specific primers showed that REA mycobionts were present in some of the screened samples while Sebacinaceae were present in all samples. These results suggest that Gaultheria spp. from NW Patagonia form ericoid mycorrhizae predominantly with the difficult-to-cultivate Sebacinaceae while the incidence of REA is relatively low and may be masked by other most likely non-mycorrhizal cultivable mycobionts.
Fil: Bruzone, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Fontenla, Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Vohnik, Martin. Charles University; República Checa
description Ericaceae diversity hotspots are in the mountains of the Neotropics and Papua New Guinea, South Africa’s fynbos and Southeast Asia but majority of references to their root mycobionts come from the Northern Hemisphere. Here, typical cultivable ericoid mycorrhizal (ErM) fungi comprise Rhizoscyphus ericae, Meliniomyces variabilis, and Oidiodendron maius. It is however unclear whether this is true also for the Southern Hemisphere. Our study focused on cultivable mycobionts from hair roots of Gaultheria mucronata and Gaultheria poeppigii (Ericaceae) from two natural forests in NW Patagonia, Argentina, differing in mycorrhizal preferences of their tree dominants. We detected 62 well-defined OTUs mostly belonging to Helotiales and Hypocreales; the most frequent were Phialocephala fortinii s. l., Pochonia suchlasporia, and Ilyonectria radicicola. Only one out of 257 isolates showed ITS nrDNA similarity to members of the R. ericae aggregate (REA) but was not conspecific with R. ericae, and only five isolates were conspecific with O. maius. Microscopic observations showed that the screened roots were frequently colonized in a manner differing from the pattern typically produced by R. ericae and O. maius. A re-synthesis experiment with selected isolates showed that only O. maius formed colonization resembling ericoid mycorrhiza. Amplification of root fungal DNA with REA-specific and Sebacinaceae-specific primers showed that REA mycobionts were present in some of the screened samples while Sebacinaceae were present in all samples. These results suggest that Gaultheria spp. from NW Patagonia form ericoid mycorrhizae predominantly with the difficult-to-cultivate Sebacinaceae while the incidence of REA is relatively low and may be masked by other most likely non-mycorrhizal cultivable mycobionts.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32692
Vohnik, Martin; Fontenla, Sonia; Bruzone, María Clara; Is the prominent ericoid mycorrhizal fungus Rhizoscyphus ericae absent in the Southern Hemisphere’s Ericaceae? A case study on the diversity of root mycobionts in Gaultheria spp. from northwest Patagonia, Argentina; Springer; Mycorrhiza; 25; 1; 5-2014; 25-40
0940-6360
1432-1890
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32692
identifier_str_mv Vohnik, Martin; Fontenla, Sonia; Bruzone, María Clara; Is the prominent ericoid mycorrhizal fungus Rhizoscyphus ericae absent in the Southern Hemisphere’s Ericaceae? A case study on the diversity of root mycobionts in Gaultheria spp. from northwest Patagonia, Argentina; Springer; Mycorrhiza; 25; 1; 5-2014; 25-40
0940-6360
1432-1890
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00572-014-0586-3
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00572-014-0586-3
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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