High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow
- Autores
- Salamone, Daniel Felipe; Barañao, Jose Lino Salvador; Santos, Claudio; Bussmann, Leonardo Edmundo; Artuso, Jorge; Werning, Carlos; Prync, Aida; Carbonetto, Cesar; Dabsys, Susana; Munar, Carlos; Salaberry R; Berra, Guillermo; Berra, Ignacio; Fernández, Nahuel; Papouchado, Mariana; Foti, Marcelo; Judewicz, Norberto; Mujica, Ignacio; Muñoz, Luciana; Fernandez Alvarez, Silvina; González, Eliseo; Zimmermann, Juan; Criscuolo, Marcelo; Melo, Carlos
- Año de publicación
- 2006
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Transgenic farm animals have been proposed as an alternative to current bioreactors for large scale production of biopharmaceuticals. However, the efficiency of both methods in the production of the same protein has not yet been established. Here we report the production of recombinant human growth hormone (hGH) in the milk of a cloned transgenic cow at levels of up to 5 g l(-1). The hormone is identical to that currently produced by expression in E. coli. In addition, the hematological and somatometric parameters of the cloned transgenic cow are within the normal range for the breed and it is fertile and capable of producing normal offspring. These results demonstrate that transgenic cattle can be used as a cost-effective alternative for the production of this hormone.
Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Biosidus ; Argentina
Fil: Barañao, Jose Lino Salvador. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Santos, Claudio. Biosidus ; Argentina
Fil: Bussmann, Leonardo Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Artuso, Jorge. Biosidus; Argentina
Fil: Werning, Carlos. Biosidus; Argentina
Fil: Prync, Aida. Biosidus; Argentina
Fil: Carbonetto, Cesar. Biosidus; Argentina
Fil: Dabsys, Susana. Biosidus; Argentina
Fil: Munar, Carlos. Munar y Asociados; Argentina
Fil: Salaberry R. Biosidus; Argentina
Fil: Berra, Guillermo. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Berra, Ignacio. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Fernández, Nahuel. Biosidus ; Argentina
Fil: Papouchado, Mariana. Biosidus; Argentina
Fil: Foti, Marcelo. Biosidus; Argentina
Fil: Judewicz, Norberto. Biosidus; Argentina
Fil: Mujica, Ignacio. Munar y Asociados; Argentina
Fil: Muñoz, Luciana. Biosidus; Argentina
Fil: Fernandez Alvarez, Silvina. Biosidus; Argentina
Fil: González, Eliseo. Centro de Diagnóstico Molecular; Argentina
Fil: Zimmermann, Juan. Biosidus; Argentina
Fil: Criscuolo, Marcelo. Biosidus; Argentina
Fil: Melo, Carlos. Biosidus; Argentina - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/27527
Ver los metadatos del registro completo
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High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cowSalamone, Daniel FelipeBarañao, Jose Lino SalvadorSantos, ClaudioBussmann, Leonardo EdmundoArtuso, JorgeWerning, CarlosPrync, AidaCarbonetto, CesarDabsys, SusanaMunar, CarlosSalaberry RBerra, GuillermoBerra, IgnacioFernández, NahuelPapouchado, MarianaFoti, MarceloJudewicz, NorbertoMujica, IgnacioMuñoz, LucianaFernandez Alvarez, SilvinaGonzález, EliseoZimmermann, JuanCriscuolo, MarceloMelo, Carloshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Transgenic farm animals have been proposed as an alternative to current bioreactors for large scale production of biopharmaceuticals. However, the efficiency of both methods in the production of the same protein has not yet been established. Here we report the production of recombinant human growth hormone (hGH) in the milk of a cloned transgenic cow at levels of up to 5 g l(-1). The hormone is identical to that currently produced by expression in E. coli. In addition, the hematological and somatometric parameters of the cloned transgenic cow are within the normal range for the breed and it is fertile and capable of producing normal offspring. These results demonstrate that transgenic cattle can be used as a cost-effective alternative for the production of this hormone.Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Biosidus ; ArgentinaFil: Barañao, Jose Lino Salvador. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Santos, Claudio. Biosidus ; ArgentinaFil: Bussmann, Leonardo Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Artuso, Jorge. Biosidus; ArgentinaFil: Werning, Carlos. Biosidus; ArgentinaFil: Prync, Aida. Biosidus; ArgentinaFil: Carbonetto, Cesar. Biosidus; ArgentinaFil: Dabsys, Susana. Biosidus; ArgentinaFil: Munar, Carlos. Munar y Asociados; ArgentinaFil: Salaberry R. Biosidus; ArgentinaFil: Berra, Guillermo. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Berra, Ignacio. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Fernández, Nahuel. Biosidus ; ArgentinaFil: Papouchado, Mariana. Biosidus; ArgentinaFil: Foti, Marcelo. Biosidus; ArgentinaFil: Judewicz, Norberto. Biosidus; ArgentinaFil: Mujica, Ignacio. Munar y Asociados; ArgentinaFil: Muñoz, Luciana. Biosidus; ArgentinaFil: Fernandez Alvarez, Silvina. Biosidus; ArgentinaFil: González, Eliseo. Centro de Diagnóstico Molecular; ArgentinaFil: Zimmermann, Juan. Biosidus; ArgentinaFil: Criscuolo, Marcelo. Biosidus; ArgentinaFil: Melo, Carlos. Biosidus; ArgentinaElsevier Science2006-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/27527Salamone, Daniel Felipe; Barañao, Jose Lino Salvador; Santos, Claudio; Bussmann, Leonardo Edmundo; Artuso, Jorge; et al.; High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow; Elsevier Science; Journal of Biotechnology; 124; 2; 7-2006; 469-4720168-16561873-4863CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0168165606000484?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jbiotec.2006.01.005info:eu-repo/semantics/altIdentifier/pmid/16716426info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:22:46Zoai:ri.conicet.gov.ar:11336/27527instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:22:46.802CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow |
| title |
High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow |
| spellingShingle |
High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow Salamone, Daniel Felipe |
| title_short |
High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow |
| title_full |
High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow |
| title_fullStr |
High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow |
| title_full_unstemmed |
High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow |
| title_sort |
High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow |
| dc.creator.none.fl_str_mv |
Salamone, Daniel Felipe Barañao, Jose Lino Salvador Santos, Claudio Bussmann, Leonardo Edmundo Artuso, Jorge Werning, Carlos Prync, Aida Carbonetto, Cesar Dabsys, Susana Munar, Carlos Salaberry R Berra, Guillermo Berra, Ignacio Fernández, Nahuel Papouchado, Mariana Foti, Marcelo Judewicz, Norberto Mujica, Ignacio Muñoz, Luciana Fernandez Alvarez, Silvina González, Eliseo Zimmermann, Juan Criscuolo, Marcelo Melo, Carlos |
| author |
Salamone, Daniel Felipe |
| author_facet |
Salamone, Daniel Felipe Barañao, Jose Lino Salvador Santos, Claudio Bussmann, Leonardo Edmundo Artuso, Jorge Werning, Carlos Prync, Aida Carbonetto, Cesar Dabsys, Susana Munar, Carlos Salaberry R Berra, Guillermo Berra, Ignacio Fernández, Nahuel Papouchado, Mariana Foti, Marcelo Judewicz, Norberto Mujica, Ignacio Muñoz, Luciana Fernandez Alvarez, Silvina González, Eliseo Zimmermann, Juan Criscuolo, Marcelo Melo, Carlos |
| author_role |
author |
| author2 |
Barañao, Jose Lino Salvador Santos, Claudio Bussmann, Leonardo Edmundo Artuso, Jorge Werning, Carlos Prync, Aida Carbonetto, Cesar Dabsys, Susana Munar, Carlos Salaberry R Berra, Guillermo Berra, Ignacio Fernández, Nahuel Papouchado, Mariana Foti, Marcelo Judewicz, Norberto Mujica, Ignacio Muñoz, Luciana Fernandez Alvarez, Silvina González, Eliseo Zimmermann, Juan Criscuolo, Marcelo Melo, Carlos |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 |
| dc.description.none.fl_txt_mv |
Transgenic farm animals have been proposed as an alternative to current bioreactors for large scale production of biopharmaceuticals. However, the efficiency of both methods in the production of the same protein has not yet been established. Here we report the production of recombinant human growth hormone (hGH) in the milk of a cloned transgenic cow at levels of up to 5 g l(-1). The hormone is identical to that currently produced by expression in E. coli. In addition, the hematological and somatometric parameters of the cloned transgenic cow are within the normal range for the breed and it is fertile and capable of producing normal offspring. These results demonstrate that transgenic cattle can be used as a cost-effective alternative for the production of this hormone. Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Biosidus ; Argentina Fil: Barañao, Jose Lino Salvador. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Santos, Claudio. Biosidus ; Argentina Fil: Bussmann, Leonardo Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Artuso, Jorge. Biosidus; Argentina Fil: Werning, Carlos. Biosidus; Argentina Fil: Prync, Aida. Biosidus; Argentina Fil: Carbonetto, Cesar. Biosidus; Argentina Fil: Dabsys, Susana. Biosidus; Argentina Fil: Munar, Carlos. Munar y Asociados; Argentina Fil: Salaberry R. Biosidus; Argentina Fil: Berra, Guillermo. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Berra, Ignacio. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Fernández, Nahuel. Biosidus ; Argentina Fil: Papouchado, Mariana. Biosidus; Argentina Fil: Foti, Marcelo. Biosidus; Argentina Fil: Judewicz, Norberto. Biosidus; Argentina Fil: Mujica, Ignacio. Munar y Asociados; Argentina Fil: Muñoz, Luciana. Biosidus; Argentina Fil: Fernandez Alvarez, Silvina. Biosidus; Argentina Fil: González, Eliseo. Centro de Diagnóstico Molecular; Argentina Fil: Zimmermann, Juan. Biosidus; Argentina Fil: Criscuolo, Marcelo. Biosidus; Argentina Fil: Melo, Carlos. Biosidus; Argentina |
| description |
Transgenic farm animals have been proposed as an alternative to current bioreactors for large scale production of biopharmaceuticals. However, the efficiency of both methods in the production of the same protein has not yet been established. Here we report the production of recombinant human growth hormone (hGH) in the milk of a cloned transgenic cow at levels of up to 5 g l(-1). The hormone is identical to that currently produced by expression in E. coli. In addition, the hematological and somatometric parameters of the cloned transgenic cow are within the normal range for the breed and it is fertile and capable of producing normal offspring. These results demonstrate that transgenic cattle can be used as a cost-effective alternative for the production of this hormone. |
| publishDate |
2006 |
| dc.date.none.fl_str_mv |
2006-07 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/27527 Salamone, Daniel Felipe; Barañao, Jose Lino Salvador; Santos, Claudio; Bussmann, Leonardo Edmundo; Artuso, Jorge; et al.; High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow; Elsevier Science; Journal of Biotechnology; 124; 2; 7-2006; 469-472 0168-1656 1873-4863 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/27527 |
| identifier_str_mv |
Salamone, Daniel Felipe; Barañao, Jose Lino Salvador; Santos, Claudio; Bussmann, Leonardo Edmundo; Artuso, Jorge; et al.; High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow; Elsevier Science; Journal of Biotechnology; 124; 2; 7-2006; 469-472 0168-1656 1873-4863 CONICET Digital CONICET |
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eng |
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eng |
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Elsevier Science |
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Elsevier Science |
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