New roles for old friends: a microtubule-localized COP1-interacting protein promotes hypocotyl elongation in the dark
- Autores
- Arico, Denise Soledad; Wengier, Diego Leonardo; Castro, Luciana; Muschietti, Jorge Prometeo; Mazzella, Maria Agustina
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Plant irritability for light stimuli becomes crucial to cope with ambient fluctuations in order to keep up homeostasis and accomplish the life cyclesuccessfully. Light environment governs plant development. Perception of light is carried out by photoreceptors, such as phytochromes (phyA to phyE) that absorb primarily in red and far-red; and cryptochromes (cry1 to cry3) that are predominantly blue-light receptors. Once perceived, plants are able to integrate light signals into biochemical networks that conduce to proper response. Transducing these lightsignals involve changes in the phosphorylation state of proteins. In an early light-induced phosphoproteome study in Arabidopsis thaliana, we identified a protein that presents light-responsive dephosphorylation in the presence of photoactivated photoreceptors. This protein was particularly interesting because it was reported to interact with the key repressor of photomorphogenesis COP1 and thus, it is potentially involved in early photomorphogenesis events. In vivo assays with a transcriptional reporter revealed it is expressed in cotyledons and elongation zones of hypocotyl and root. Its expression is regulated negatively by light. CRISPR-CAS9 mutated lines exhibit shorter hypocotyls in darkness. Confocal microscopy assays with stably transgenic lines expressing translational reporters revealed localization to cortical microtubules. We are currently studying the biological implications of its microtubule association and its regulation by COP1 through changes in phosphorylation patterns. All these results suggest this protein promotes growth in darkness by affecting microtubules dynamics.
Fil: Arico, Denise Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Wengier, Diego Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Castro, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Mazzella, Maria Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Joint LV Annual SAIB Meeting and XIV PABMB Congress
Salta
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular - Materia
-
PHOTOMORPHOGENESIS
PHOSPHORYLATION
MICROTUBULES
COP1-INTERACTING-PROTEIN - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/234316
Ver los metadatos del registro completo
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New roles for old friends: a microtubule-localized COP1-interacting protein promotes hypocotyl elongation in the darkArico, Denise SoledadWengier, Diego LeonardoCastro, LucianaMuschietti, Jorge PrometeoMazzella, Maria AgustinaPHOTOMORPHOGENESISPHOSPHORYLATIONMICROTUBULESCOP1-INTERACTING-PROTEINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Plant irritability for light stimuli becomes crucial to cope with ambient fluctuations in order to keep up homeostasis and accomplish the life cyclesuccessfully. Light environment governs plant development. Perception of light is carried out by photoreceptors, such as phytochromes (phyA to phyE) that absorb primarily in red and far-red; and cryptochromes (cry1 to cry3) that are predominantly blue-light receptors. Once perceived, plants are able to integrate light signals into biochemical networks that conduce to proper response. Transducing these lightsignals involve changes in the phosphorylation state of proteins. In an early light-induced phosphoproteome study in Arabidopsis thaliana, we identified a protein that presents light-responsive dephosphorylation in the presence of photoactivated photoreceptors. This protein was particularly interesting because it was reported to interact with the key repressor of photomorphogenesis COP1 and thus, it is potentially involved in early photomorphogenesis events. In vivo assays with a transcriptional reporter revealed it is expressed in cotyledons and elongation zones of hypocotyl and root. Its expression is regulated negatively by light. CRISPR-CAS9 mutated lines exhibit shorter hypocotyls in darkness. Confocal microscopy assays with stably transgenic lines expressing translational reporters revealed localization to cortical microtubules. We are currently studying the biological implications of its microtubule association and its regulation by COP1 through changes in phosphorylation patterns. All these results suggest this protein promotes growth in darkness by affecting microtubules dynamics.Fil: Arico, Denise Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Wengier, Diego Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Castro, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Mazzella, Maria Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaJoint LV Annual SAIB Meeting and XIV PABMB CongressSaltaArgentinaSociedad Argentina de Investigaciones en Bioquímica y Biología MolecularTech Science Press2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/234316New roles for old friends: a microtubule-localized COP1-interacting protein promotes hypocotyl elongation in the dark; Joint LV Annual SAIB Meeting and XIV PABMB Congress; Salta; Argentina; 2019; 55-561667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019-version-final.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:32:50Zoai:ri.conicet.gov.ar:11336/234316instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:32:50.568CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
New roles for old friends: a microtubule-localized COP1-interacting protein promotes hypocotyl elongation in the dark |
title |
New roles for old friends: a microtubule-localized COP1-interacting protein promotes hypocotyl elongation in the dark |
spellingShingle |
New roles for old friends: a microtubule-localized COP1-interacting protein promotes hypocotyl elongation in the dark Arico, Denise Soledad PHOTOMORPHOGENESIS PHOSPHORYLATION MICROTUBULES COP1-INTERACTING-PROTEIN |
title_short |
New roles for old friends: a microtubule-localized COP1-interacting protein promotes hypocotyl elongation in the dark |
title_full |
New roles for old friends: a microtubule-localized COP1-interacting protein promotes hypocotyl elongation in the dark |
title_fullStr |
New roles for old friends: a microtubule-localized COP1-interacting protein promotes hypocotyl elongation in the dark |
title_full_unstemmed |
New roles for old friends: a microtubule-localized COP1-interacting protein promotes hypocotyl elongation in the dark |
title_sort |
New roles for old friends: a microtubule-localized COP1-interacting protein promotes hypocotyl elongation in the dark |
dc.creator.none.fl_str_mv |
Arico, Denise Soledad Wengier, Diego Leonardo Castro, Luciana Muschietti, Jorge Prometeo Mazzella, Maria Agustina |
author |
Arico, Denise Soledad |
author_facet |
Arico, Denise Soledad Wengier, Diego Leonardo Castro, Luciana Muschietti, Jorge Prometeo Mazzella, Maria Agustina |
author_role |
author |
author2 |
Wengier, Diego Leonardo Castro, Luciana Muschietti, Jorge Prometeo Mazzella, Maria Agustina |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
PHOTOMORPHOGENESIS PHOSPHORYLATION MICROTUBULES COP1-INTERACTING-PROTEIN |
topic |
PHOTOMORPHOGENESIS PHOSPHORYLATION MICROTUBULES COP1-INTERACTING-PROTEIN |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Plant irritability for light stimuli becomes crucial to cope with ambient fluctuations in order to keep up homeostasis and accomplish the life cyclesuccessfully. Light environment governs plant development. Perception of light is carried out by photoreceptors, such as phytochromes (phyA to phyE) that absorb primarily in red and far-red; and cryptochromes (cry1 to cry3) that are predominantly blue-light receptors. Once perceived, plants are able to integrate light signals into biochemical networks that conduce to proper response. Transducing these lightsignals involve changes in the phosphorylation state of proteins. In an early light-induced phosphoproteome study in Arabidopsis thaliana, we identified a protein that presents light-responsive dephosphorylation in the presence of photoactivated photoreceptors. This protein was particularly interesting because it was reported to interact with the key repressor of photomorphogenesis COP1 and thus, it is potentially involved in early photomorphogenesis events. In vivo assays with a transcriptional reporter revealed it is expressed in cotyledons and elongation zones of hypocotyl and root. Its expression is regulated negatively by light. CRISPR-CAS9 mutated lines exhibit shorter hypocotyls in darkness. Confocal microscopy assays with stably transgenic lines expressing translational reporters revealed localization to cortical microtubules. We are currently studying the biological implications of its microtubule association and its regulation by COP1 through changes in phosphorylation patterns. All these results suggest this protein promotes growth in darkness by affecting microtubules dynamics. Fil: Arico, Denise Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Wengier, Diego Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Castro, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Mazzella, Maria Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Joint LV Annual SAIB Meeting and XIV PABMB Congress Salta Argentina Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular |
description |
Plant irritability for light stimuli becomes crucial to cope with ambient fluctuations in order to keep up homeostasis and accomplish the life cyclesuccessfully. Light environment governs plant development. Perception of light is carried out by photoreceptors, such as phytochromes (phyA to phyE) that absorb primarily in red and far-red; and cryptochromes (cry1 to cry3) that are predominantly blue-light receptors. Once perceived, plants are able to integrate light signals into biochemical networks that conduce to proper response. Transducing these lightsignals involve changes in the phosphorylation state of proteins. In an early light-induced phosphoproteome study in Arabidopsis thaliana, we identified a protein that presents light-responsive dephosphorylation in the presence of photoactivated photoreceptors. This protein was particularly interesting because it was reported to interact with the key repressor of photomorphogenesis COP1 and thus, it is potentially involved in early photomorphogenesis events. In vivo assays with a transcriptional reporter revealed it is expressed in cotyledons and elongation zones of hypocotyl and root. Its expression is regulated negatively by light. CRISPR-CAS9 mutated lines exhibit shorter hypocotyls in darkness. Confocal microscopy assays with stably transgenic lines expressing translational reporters revealed localization to cortical microtubules. We are currently studying the biological implications of its microtubule association and its regulation by COP1 through changes in phosphorylation patterns. All these results suggest this protein promotes growth in darkness by affecting microtubules dynamics. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/234316 New roles for old friends: a microtubule-localized COP1-interacting protein promotes hypocotyl elongation in the dark; Joint LV Annual SAIB Meeting and XIV PABMB Congress; Salta; Argentina; 2019; 55-56 1667-5746 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/234316 |
identifier_str_mv |
New roles for old friends: a microtubule-localized COP1-interacting protein promotes hypocotyl elongation in the dark; Joint LV Annual SAIB Meeting and XIV PABMB Congress; Salta; Argentina; 2019; 55-56 1667-5746 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019-version-final.pdf |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Internacional |
dc.publisher.none.fl_str_mv |
Tech Science Press |
publisher.none.fl_str_mv |
Tech Science Press |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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