A novel mechanosensitive channel controls osmoregulation, differentiation, and infectivity in trypanosoma cruzi

Autores
Dave, Noopur; Cetiner, Ugur; Arroyo, Daniel; Fonbuena, Joshua; Tiwari, Megna; Barrera, Patricia Andrea; Lander, Noelia; Anishkin, Andriy; Sukharev, Sergei; Jimenez, Veronica
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The causative agent of Chagas disease undergoes drastic morphological and biochemical modifications as it passes between hosts and transitions from extracellular to intracellular stages. The osmotic and mechanical aspects of these cellular transformations are not understood. Here we identify and characterize a novel mechanosensitive channel in Trypanosoma cruzi (TcMscS) belonging to the superfamily of small-conductance mechanosensitive channels (MscS). TcMscS is activated by membrane tension and forms a large pore permeable to anions, cations, and small osmolytes. The channel changes its location from the contractile vacuole complex in epimastigotes to the plasma membrane as the parasites develop into intracellular amastigotes. TcMscS knockout parasites show significant fitness defects, including increased cell volume, calcium dysregulation, impaired differentiation, and a dramatic decrease in infectivity. Our work provides mechanistic insights into components supporting pathogen adaptation inside the host, thus opening the exploration of mechanosensation as a prerequisite for protozoan infectivity.
Fil: Dave, Noopur. California State University; Estados Unidos
Fil: Cetiner, Ugur. University of Maryland; Estados Unidos
Fil: Arroyo, Daniel. California State University; Estados Unidos
Fil: Fonbuena, Joshua. California State University; Estados Unidos
Fil: Tiwari, Megna. California State University; Estados Unidos
Fil: Barrera, Patricia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Lander, Noelia. University of Cincinnati; Estados Unidos
Fil: Anishkin, Andriy. University of Maryland; Estados Unidos
Fil: Sukharev, Sergei. University of Maryland; Estados Unidos
Fil: Jimenez, Veronica. California State University; Estados Unidos
Materia
TRYPANOSOMA CRUZI
MECHANOSENSATION
CRISPR-CAS9
INFECTIVITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/174639
Acceder
id CONICETDig_edd8b2b9c6fe8329ad5aff8e8b7a100f
oai_identifier_str oai:ri.conicet.gov.ar:11336/174639
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A novel mechanosensitive channel controls osmoregulation, differentiation, and infectivity in trypanosoma cruziDave, NoopurCetiner, UgurArroyo, DanielFonbuena, JoshuaTiwari, MegnaBarrera, Patricia AndreaLander, NoeliaAnishkin, AndriySukharev, SergeiJimenez, VeronicaTRYPANOSOMA CRUZIMECHANOSENSATIONCRISPR-CAS9INFECTIVITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The causative agent of Chagas disease undergoes drastic morphological and biochemical modifications as it passes between hosts and transitions from extracellular to intracellular stages. The osmotic and mechanical aspects of these cellular transformations are not understood. Here we identify and characterize a novel mechanosensitive channel in Trypanosoma cruzi (TcMscS) belonging to the superfamily of small-conductance mechanosensitive channels (MscS). TcMscS is activated by membrane tension and forms a large pore permeable to anions, cations, and small osmolytes. The channel changes its location from the contractile vacuole complex in epimastigotes to the plasma membrane as the parasites develop into intracellular amastigotes. TcMscS knockout parasites show significant fitness defects, including increased cell volume, calcium dysregulation, impaired differentiation, and a dramatic decrease in infectivity. Our work provides mechanistic insights into components supporting pathogen adaptation inside the host, thus opening the exploration of mechanosensation as a prerequisite for protozoan infectivity.Fil: Dave, Noopur. California State University; Estados UnidosFil: Cetiner, Ugur. University of Maryland; Estados UnidosFil: Arroyo, Daniel. California State University; Estados UnidosFil: Fonbuena, Joshua. California State University; Estados UnidosFil: Tiwari, Megna. California State University; Estados UnidosFil: Barrera, Patricia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Lander, Noelia. University of Cincinnati; Estados UnidosFil: Anishkin, Andriy. University of Maryland; Estados UnidosFil: Sukharev, Sergei. University of Maryland; Estados UnidosFil: Jimenez, Veronica. California State University; Estados UnidoseLife Sciences Publications2021-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/174639Dave, Noopur; Cetiner, Ugur; Arroyo, Daniel; Fonbuena, Joshua; Tiwari, Megna; et al.; A novel mechanosensitive channel controls osmoregulation, differentiation, and infectivity in trypanosoma cruzi; eLife Sciences Publications; eLife; 10; 7-2021; 1-322050-084XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/67449info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.67449info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:05Zoai:ri.conicet.gov.ar:11336/174639instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:05.516CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A novel mechanosensitive channel controls osmoregulation, differentiation, and infectivity in trypanosoma cruzi
title A novel mechanosensitive channel controls osmoregulation, differentiation, and infectivity in trypanosoma cruzi
spellingShingle A novel mechanosensitive channel controls osmoregulation, differentiation, and infectivity in trypanosoma cruzi
Dave, Noopur
TRYPANOSOMA CRUZI
MECHANOSENSATION
CRISPR-CAS9
INFECTIVITY
title_short A novel mechanosensitive channel controls osmoregulation, differentiation, and infectivity in trypanosoma cruzi
title_full A novel mechanosensitive channel controls osmoregulation, differentiation, and infectivity in trypanosoma cruzi
title_fullStr A novel mechanosensitive channel controls osmoregulation, differentiation, and infectivity in trypanosoma cruzi
title_full_unstemmed A novel mechanosensitive channel controls osmoregulation, differentiation, and infectivity in trypanosoma cruzi
title_sort A novel mechanosensitive channel controls osmoregulation, differentiation, and infectivity in trypanosoma cruzi
dc.creator.none.fl_str_mv Dave, Noopur
Cetiner, Ugur
Arroyo, Daniel
Fonbuena, Joshua
Tiwari, Megna
Barrera, Patricia Andrea
Lander, Noelia
Anishkin, Andriy
Sukharev, Sergei
Jimenez, Veronica
author Dave, Noopur
author_facet Dave, Noopur
Cetiner, Ugur
Arroyo, Daniel
Fonbuena, Joshua
Tiwari, Megna
Barrera, Patricia Andrea
Lander, Noelia
Anishkin, Andriy
Sukharev, Sergei
Jimenez, Veronica
author_role author
author2 Cetiner, Ugur
Arroyo, Daniel
Fonbuena, Joshua
Tiwari, Megna
Barrera, Patricia Andrea
Lander, Noelia
Anishkin, Andriy
Sukharev, Sergei
Jimenez, Veronica
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TRYPANOSOMA CRUZI
MECHANOSENSATION
CRISPR-CAS9
INFECTIVITY
topic TRYPANOSOMA CRUZI
MECHANOSENSATION
CRISPR-CAS9
INFECTIVITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The causative agent of Chagas disease undergoes drastic morphological and biochemical modifications as it passes between hosts and transitions from extracellular to intracellular stages. The osmotic and mechanical aspects of these cellular transformations are not understood. Here we identify and characterize a novel mechanosensitive channel in Trypanosoma cruzi (TcMscS) belonging to the superfamily of small-conductance mechanosensitive channels (MscS). TcMscS is activated by membrane tension and forms a large pore permeable to anions, cations, and small osmolytes. The channel changes its location from the contractile vacuole complex in epimastigotes to the plasma membrane as the parasites develop into intracellular amastigotes. TcMscS knockout parasites show significant fitness defects, including increased cell volume, calcium dysregulation, impaired differentiation, and a dramatic decrease in infectivity. Our work provides mechanistic insights into components supporting pathogen adaptation inside the host, thus opening the exploration of mechanosensation as a prerequisite for protozoan infectivity.
Fil: Dave, Noopur. California State University; Estados Unidos
Fil: Cetiner, Ugur. University of Maryland; Estados Unidos
Fil: Arroyo, Daniel. California State University; Estados Unidos
Fil: Fonbuena, Joshua. California State University; Estados Unidos
Fil: Tiwari, Megna. California State University; Estados Unidos
Fil: Barrera, Patricia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Lander, Noelia. University of Cincinnati; Estados Unidos
Fil: Anishkin, Andriy. University of Maryland; Estados Unidos
Fil: Sukharev, Sergei. University of Maryland; Estados Unidos
Fil: Jimenez, Veronica. California State University; Estados Unidos
description The causative agent of Chagas disease undergoes drastic morphological and biochemical modifications as it passes between hosts and transitions from extracellular to intracellular stages. The osmotic and mechanical aspects of these cellular transformations are not understood. Here we identify and characterize a novel mechanosensitive channel in Trypanosoma cruzi (TcMscS) belonging to the superfamily of small-conductance mechanosensitive channels (MscS). TcMscS is activated by membrane tension and forms a large pore permeable to anions, cations, and small osmolytes. The channel changes its location from the contractile vacuole complex in epimastigotes to the plasma membrane as the parasites develop into intracellular amastigotes. TcMscS knockout parasites show significant fitness defects, including increased cell volume, calcium dysregulation, impaired differentiation, and a dramatic decrease in infectivity. Our work provides mechanistic insights into components supporting pathogen adaptation inside the host, thus opening the exploration of mechanosensation as a prerequisite for protozoan infectivity.
publishDate 2021
dc.date.none.fl_str_mv 2021-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/174639
Dave, Noopur; Cetiner, Ugur; Arroyo, Daniel; Fonbuena, Joshua; Tiwari, Megna; et al.; A novel mechanosensitive channel controls osmoregulation, differentiation, and infectivity in trypanosoma cruzi; eLife Sciences Publications; eLife; 10; 7-2021; 1-32
2050-084X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/174639
identifier_str_mv Dave, Noopur; Cetiner, Ugur; Arroyo, Daniel; Fonbuena, Joshua; Tiwari, Megna; et al.; A novel mechanosensitive channel controls osmoregulation, differentiation, and infectivity in trypanosoma cruzi; eLife Sciences Publications; eLife; 10; 7-2021; 1-32
2050-084X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/67449
info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.67449
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv eLife Sciences Publications
publisher.none.fl_str_mv eLife Sciences Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269836308119552
score 13.13397

Publicaciones similares