The peril of PCR inhibitors in environmental samples: the case of Didymosphenia geminata

Autores
Jones, Leandro Roberto; Uyua, Noelia Mariel; Manrique, Julieta Marina
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Since the introduction of polymerase chain reaction (PCR) biodiversity study has been significantly influenced by the chance of generating unprecedented amounts of molecular data. Although it is a robust technique, those applications requiring high sensitivity and reproducibility, that is PCR detection and quantitative PCR, can be negatively affected by PCR inhibition. This is particularly challenging for diverse kinds of samples included the environmental ones, which usually contain complex mixtures of a variety of inhibitory substances. The problem of PCR inhibition can be overcome, or ameliorated, by implementing adequate protocols of nucleic acids purification, internal controls and modern analytical approaches focused on PCR kinetics. Herein, we remark these procedures and describe the general techniques that can be used to optimize DNA extraction protocols for PCR applications. In addition, we show that PCR inhibition might have negative consequences in molecular studies of Didymosphenia geminata, an invasive microalga that have recently developed massive blooms in temperate regions worldwide, and provide general guidelines for dealing with this problem.
Fil: Jones, Leandro Roberto. Universidad Nacional de la Patagonia ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Uyua, Noelia Mariel. Universidad Nacional de la Patagonia ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Manrique, Julieta Marina. Universidad Nacional de la Patagonia ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Didymosphenia
Molecular Detection
Pcr
Rock Snot
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37733
Acceder
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network_name_str CONICET Digital (CONICET)
spelling The peril of PCR inhibitors in environmental samples: the case of Didymosphenia geminataJones, Leandro RobertoUyua, Noelia MarielManrique, Julieta MarinaDidymospheniaMolecular DetectionPcrRock Snothttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Since the introduction of polymerase chain reaction (PCR) biodiversity study has been significantly influenced by the chance of generating unprecedented amounts of molecular data. Although it is a robust technique, those applications requiring high sensitivity and reproducibility, that is PCR detection and quantitative PCR, can be negatively affected by PCR inhibition. This is particularly challenging for diverse kinds of samples included the environmental ones, which usually contain complex mixtures of a variety of inhibitory substances. The problem of PCR inhibition can be overcome, or ameliorated, by implementing adequate protocols of nucleic acids purification, internal controls and modern analytical approaches focused on PCR kinetics. Herein, we remark these procedures and describe the general techniques that can be used to optimize DNA extraction protocols for PCR applications. In addition, we show that PCR inhibition might have negative consequences in molecular studies of Didymosphenia geminata, an invasive microalga that have recently developed massive blooms in temperate regions worldwide, and provide general guidelines for dealing with this problem.Fil: Jones, Leandro Roberto. Universidad Nacional de la Patagonia ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Uyua, Noelia Mariel. Universidad Nacional de la Patagonia ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Manrique, Julieta Marina. Universidad Nacional de la Patagonia ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37733Jones, Leandro Roberto; Uyua, Noelia Mariel; Manrique, Julieta Marina; The peril of PCR inhibitors in environmental samples: the case of Didymosphenia geminata; Springer; Biodiversity and Conservation; 24; 6; 6-2015; 1541-15480960-31151572-9710CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10531-014-0849-5info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10531-014-0849-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:45:00Zoai:ri.conicet.gov.ar:11336/37733instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:45:00.931CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The peril of PCR inhibitors in environmental samples: the case of Didymosphenia geminata
title The peril of PCR inhibitors in environmental samples: the case of Didymosphenia geminata
spellingShingle The peril of PCR inhibitors in environmental samples: the case of Didymosphenia geminata
Jones, Leandro Roberto
Didymosphenia
Molecular Detection
Pcr
Rock Snot
title_short The peril of PCR inhibitors in environmental samples: the case of Didymosphenia geminata
title_full The peril of PCR inhibitors in environmental samples: the case of Didymosphenia geminata
title_fullStr The peril of PCR inhibitors in environmental samples: the case of Didymosphenia geminata
title_full_unstemmed The peril of PCR inhibitors in environmental samples: the case of Didymosphenia geminata
title_sort The peril of PCR inhibitors in environmental samples: the case of Didymosphenia geminata
dc.creator.none.fl_str_mv Jones, Leandro Roberto
Uyua, Noelia Mariel
Manrique, Julieta Marina
author Jones, Leandro Roberto
author_facet Jones, Leandro Roberto
Uyua, Noelia Mariel
Manrique, Julieta Marina
author_role author
author2 Uyua, Noelia Mariel
Manrique, Julieta Marina
author2_role author
author
dc.subject.none.fl_str_mv Didymosphenia
Molecular Detection
Pcr
Rock Snot
topic Didymosphenia
Molecular Detection
Pcr
Rock Snot
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Since the introduction of polymerase chain reaction (PCR) biodiversity study has been significantly influenced by the chance of generating unprecedented amounts of molecular data. Although it is a robust technique, those applications requiring high sensitivity and reproducibility, that is PCR detection and quantitative PCR, can be negatively affected by PCR inhibition. This is particularly challenging for diverse kinds of samples included the environmental ones, which usually contain complex mixtures of a variety of inhibitory substances. The problem of PCR inhibition can be overcome, or ameliorated, by implementing adequate protocols of nucleic acids purification, internal controls and modern analytical approaches focused on PCR kinetics. Herein, we remark these procedures and describe the general techniques that can be used to optimize DNA extraction protocols for PCR applications. In addition, we show that PCR inhibition might have negative consequences in molecular studies of Didymosphenia geminata, an invasive microalga that have recently developed massive blooms in temperate regions worldwide, and provide general guidelines for dealing with this problem.
Fil: Jones, Leandro Roberto. Universidad Nacional de la Patagonia ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Uyua, Noelia Mariel. Universidad Nacional de la Patagonia ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Manrique, Julieta Marina. Universidad Nacional de la Patagonia ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Since the introduction of polymerase chain reaction (PCR) biodiversity study has been significantly influenced by the chance of generating unprecedented amounts of molecular data. Although it is a robust technique, those applications requiring high sensitivity and reproducibility, that is PCR detection and quantitative PCR, can be negatively affected by PCR inhibition. This is particularly challenging for diverse kinds of samples included the environmental ones, which usually contain complex mixtures of a variety of inhibitory substances. The problem of PCR inhibition can be overcome, or ameliorated, by implementing adequate protocols of nucleic acids purification, internal controls and modern analytical approaches focused on PCR kinetics. Herein, we remark these procedures and describe the general techniques that can be used to optimize DNA extraction protocols for PCR applications. In addition, we show that PCR inhibition might have negative consequences in molecular studies of Didymosphenia geminata, an invasive microalga that have recently developed massive blooms in temperate regions worldwide, and provide general guidelines for dealing with this problem.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37733
Jones, Leandro Roberto; Uyua, Noelia Mariel; Manrique, Julieta Marina; The peril of PCR inhibitors in environmental samples: the case of Didymosphenia geminata; Springer; Biodiversity and Conservation; 24; 6; 6-2015; 1541-1548
0960-3115
1572-9710
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37733
identifier_str_mv Jones, Leandro Roberto; Uyua, Noelia Mariel; Manrique, Julieta Marina; The peril of PCR inhibitors in environmental samples: the case of Didymosphenia geminata; Springer; Biodiversity and Conservation; 24; 6; 6-2015; 1541-1548
0960-3115
1572-9710
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s10531-014-0849-5
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10531-014-0849-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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