An optimized DNA extraction protocol for benthic Didymosphenia geminata

Autores
Uyua, Noelia Mariel; Manrique, Julieta Marina; Jones, Leandro Roberto
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Didymosphenia geminata mats display few cells in relation to extracellular material and contain polysaccharides and heavy metals that interfere with molecular studies. We describe an optimized DNA extraction protocol that help to overcome these difficulties. Our protocol outperformed five previously described DNA extraction techniques.
Fil: Uyua, Noelia Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; Argentina
Fil: Manrique, Julieta Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; Argentina
Fil: Jones, Leandro Roberto. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; Argentina
Materia
Didymosphenia
Dna Extraction
Pcr
Stalk
Benthic
Invasive
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/35693
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/35693
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An optimized DNA extraction protocol for benthic Didymosphenia geminataUyua, Noelia MarielManrique, Julieta MarinaJones, Leandro RobertoDidymospheniaDna ExtractionPcrStalkBenthicInvasivehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Didymosphenia geminata mats display few cells in relation to extracellular material and contain polysaccharides and heavy metals that interfere with molecular studies. We describe an optimized DNA extraction protocol that help to overcome these difficulties. Our protocol outperformed five previously described DNA extraction techniques.Fil: Uyua, Noelia Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; ArgentinaFil: Manrique, Julieta Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; ArgentinaFil: Jones, Leandro Roberto. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; ArgentinaElsevier Science2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/35693Uyua, Noelia Mariel; Manrique, Julieta Marina; Jones, Leandro Roberto; An optimized DNA extraction protocol for benthic Didymosphenia geminata; Elsevier Science; Journal Of Microbiological Methods; 104; 6-2014; 12-180167-7012CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.mimet.2014.06.007info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0167701214001626info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:42Zoai:ri.conicet.gov.ar:11336/35693instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:43.051CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An optimized DNA extraction protocol for benthic Didymosphenia geminata
title An optimized DNA extraction protocol for benthic Didymosphenia geminata
spellingShingle An optimized DNA extraction protocol for benthic Didymosphenia geminata
Uyua, Noelia Mariel
Didymosphenia
Dna Extraction
Pcr
Stalk
Benthic
Invasive
title_short An optimized DNA extraction protocol for benthic Didymosphenia geminata
title_full An optimized DNA extraction protocol for benthic Didymosphenia geminata
title_fullStr An optimized DNA extraction protocol for benthic Didymosphenia geminata
title_full_unstemmed An optimized DNA extraction protocol for benthic Didymosphenia geminata
title_sort An optimized DNA extraction protocol for benthic Didymosphenia geminata
dc.creator.none.fl_str_mv Uyua, Noelia Mariel
Manrique, Julieta Marina
Jones, Leandro Roberto
author Uyua, Noelia Mariel
author_facet Uyua, Noelia Mariel
Manrique, Julieta Marina
Jones, Leandro Roberto
author_role author
author2 Manrique, Julieta Marina
Jones, Leandro Roberto
author2_role author
author
dc.subject.none.fl_str_mv Didymosphenia
Dna Extraction
Pcr
Stalk
Benthic
Invasive
topic Didymosphenia
Dna Extraction
Pcr
Stalk
Benthic
Invasive
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Didymosphenia geminata mats display few cells in relation to extracellular material and contain polysaccharides and heavy metals that interfere with molecular studies. We describe an optimized DNA extraction protocol that help to overcome these difficulties. Our protocol outperformed five previously described DNA extraction techniques.
Fil: Uyua, Noelia Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; Argentina
Fil: Manrique, Julieta Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; Argentina
Fil: Jones, Leandro Roberto. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; Argentina
description Didymosphenia geminata mats display few cells in relation to extracellular material and contain polysaccharides and heavy metals that interfere with molecular studies. We describe an optimized DNA extraction protocol that help to overcome these difficulties. Our protocol outperformed five previously described DNA extraction techniques.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/35693
Uyua, Noelia Mariel; Manrique, Julieta Marina; Jones, Leandro Roberto; An optimized DNA extraction protocol for benthic Didymosphenia geminata; Elsevier Science; Journal Of Microbiological Methods; 104; 6-2014; 12-18
0167-7012
CONICET Digital
CONICET
url http://hdl.handle.net/11336/35693
identifier_str_mv Uyua, Noelia Mariel; Manrique, Julieta Marina; Jones, Leandro Roberto; An optimized DNA extraction protocol for benthic Didymosphenia geminata; Elsevier Science; Journal Of Microbiological Methods; 104; 6-2014; 12-18
0167-7012
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mimet.2014.06.007
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0167701214001626
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613486128136192
score 13.070432

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