Genotypic analysis of isolated peanut-nodulating rhizobial strains reveals differences among populations obtained from soils with different cropping histories

Autores
Nievas, Fiorela Lujan; Bogino, Pablo Cesar; Nocelli, Natalia Estefanía; Giordano, Walter Fabian
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Peanut (Arachis hypogaea) is one of the most important crops of Córdoba province, Argentina; however, knowledge of the genetic diversity of peanut-nodulating rhizobial populations is fragmentary. In view of the importance of information on native rhizobial populations in relation to improved agricultural management practices, we performed genetic characterization of rhizobia isolated from root nodules of plants grown on soils with previous peanut cropping history (PH: Río Cuarto and Cabrera sites) vs. soils with no previous peanut cropping history (NPH: La Aguada and Chaján sites). Ten different 16S rRNA restriction fragment length polymorphism (RFLP) genotypes were obtained through combination of restriction patterns obtained with four endonucleases. Cluster analysis of genotypes gave two major groups at 80% similarity. Each group contained strains from mostly unrelated origins, indicating a genetic relationship among peanut-nodulating populations isolated from different peanut cropping systems. Furthermore, sequence analysis of the 16S rRNA gene demonstrated identity of isolates with strains of the genus Bradyrhizobium, and phylogenetic association among rhizobial populations from sites with different peanut cropping histories. Diversity indexes estimated for RFLP genotypes showed that populations obtained from PH soils were less diverse than those from NPH soils, suggesting that the presence of the legume results in selection of particular rhizobial strains. Higher polymorphism and diversity were revealed by enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis. Peanut strains clustered at very low levels of similarity (55%). Populations of different origins clustered together, although at elevated genetic distance. ERIC-PCR results indicated the presence of different rhizobial populations in soils of Córdoba province.
Fil: Nievas, Fiorela Lujan. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Bogino, Pablo Cesar. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Nocelli, Natalia Estefanía. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Giordano, Walter Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Materia
16S RRNA SEQUENCING
BRADYRHIZOBIM
ERIC-PCR
GENETIC DIVERSITY
PEANUT CROPPING HISTORY
RFLP 16S RRNA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/198533
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/198533
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genotypic analysis of isolated peanut-nodulating rhizobial strains reveals differences among populations obtained from soils with different cropping historiesNievas, Fiorela LujanBogino, Pablo CesarNocelli, Natalia EstefaníaGiordano, Walter Fabian16S RRNA SEQUENCINGBRADYRHIZOBIMERIC-PCRGENETIC DIVERSITYPEANUT CROPPING HISTORYRFLP 16S RRNAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Peanut (Arachis hypogaea) is one of the most important crops of Córdoba province, Argentina; however, knowledge of the genetic diversity of peanut-nodulating rhizobial populations is fragmentary. In view of the importance of information on native rhizobial populations in relation to improved agricultural management practices, we performed genetic characterization of rhizobia isolated from root nodules of plants grown on soils with previous peanut cropping history (PH: Río Cuarto and Cabrera sites) vs. soils with no previous peanut cropping history (NPH: La Aguada and Chaján sites). Ten different 16S rRNA restriction fragment length polymorphism (RFLP) genotypes were obtained through combination of restriction patterns obtained with four endonucleases. Cluster analysis of genotypes gave two major groups at 80% similarity. Each group contained strains from mostly unrelated origins, indicating a genetic relationship among peanut-nodulating populations isolated from different peanut cropping systems. Furthermore, sequence analysis of the 16S rRNA gene demonstrated identity of isolates with strains of the genus Bradyrhizobium, and phylogenetic association among rhizobial populations from sites with different peanut cropping histories. Diversity indexes estimated for RFLP genotypes showed that populations obtained from PH soils were less diverse than those from NPH soils, suggesting that the presence of the legume results in selection of particular rhizobial strains. Higher polymorphism and diversity were revealed by enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis. Peanut strains clustered at very low levels of similarity (55%). Populations of different origins clustered together, although at elevated genetic distance. ERIC-PCR results indicated the presence of different rhizobial populations in soils of Córdoba province.Fil: Nievas, Fiorela Lujan. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Bogino, Pablo Cesar. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Nocelli, Natalia Estefanía. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Giordano, Walter Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaElsevier Science2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/198533Nievas, Fiorela Lujan; Bogino, Pablo Cesar; Nocelli, Natalia Estefanía; Giordano, Walter Fabian; Genotypic analysis of isolated peanut-nodulating rhizobial strains reveals differences among populations obtained from soils with different cropping histories; Elsevier Science; Applied Soil Ecology; 53; 1; 2-2012; 74-820929-1393CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0929139311003155info:eu-repo/semantics/altIdentifier/doi/10.1016/j.apsoil.2011.11.010info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:03Zoai:ri.conicet.gov.ar:11336/198533instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:03.345CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genotypic analysis of isolated peanut-nodulating rhizobial strains reveals differences among populations obtained from soils with different cropping histories
title Genotypic analysis of isolated peanut-nodulating rhizobial strains reveals differences among populations obtained from soils with different cropping histories
spellingShingle Genotypic analysis of isolated peanut-nodulating rhizobial strains reveals differences among populations obtained from soils with different cropping histories
Nievas, Fiorela Lujan
16S RRNA SEQUENCING
BRADYRHIZOBIM
ERIC-PCR
GENETIC DIVERSITY
PEANUT CROPPING HISTORY
RFLP 16S RRNA
title_short Genotypic analysis of isolated peanut-nodulating rhizobial strains reveals differences among populations obtained from soils with different cropping histories
title_full Genotypic analysis of isolated peanut-nodulating rhizobial strains reveals differences among populations obtained from soils with different cropping histories
title_fullStr Genotypic analysis of isolated peanut-nodulating rhizobial strains reveals differences among populations obtained from soils with different cropping histories
title_full_unstemmed Genotypic analysis of isolated peanut-nodulating rhizobial strains reveals differences among populations obtained from soils with different cropping histories
title_sort Genotypic analysis of isolated peanut-nodulating rhizobial strains reveals differences among populations obtained from soils with different cropping histories
dc.creator.none.fl_str_mv Nievas, Fiorela Lujan
Bogino, Pablo Cesar
Nocelli, Natalia Estefanía
Giordano, Walter Fabian
author Nievas, Fiorela Lujan
author_facet Nievas, Fiorela Lujan
Bogino, Pablo Cesar
Nocelli, Natalia Estefanía
Giordano, Walter Fabian
author_role author
author2 Bogino, Pablo Cesar
Nocelli, Natalia Estefanía
Giordano, Walter Fabian
author2_role author
author
author
dc.subject.none.fl_str_mv 16S RRNA SEQUENCING
BRADYRHIZOBIM
ERIC-PCR
GENETIC DIVERSITY
PEANUT CROPPING HISTORY
RFLP 16S RRNA
topic 16S RRNA SEQUENCING
BRADYRHIZOBIM
ERIC-PCR
GENETIC DIVERSITY
PEANUT CROPPING HISTORY
RFLP 16S RRNA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Peanut (Arachis hypogaea) is one of the most important crops of Córdoba province, Argentina; however, knowledge of the genetic diversity of peanut-nodulating rhizobial populations is fragmentary. In view of the importance of information on native rhizobial populations in relation to improved agricultural management practices, we performed genetic characterization of rhizobia isolated from root nodules of plants grown on soils with previous peanut cropping history (PH: Río Cuarto and Cabrera sites) vs. soils with no previous peanut cropping history (NPH: La Aguada and Chaján sites). Ten different 16S rRNA restriction fragment length polymorphism (RFLP) genotypes were obtained through combination of restriction patterns obtained with four endonucleases. Cluster analysis of genotypes gave two major groups at 80% similarity. Each group contained strains from mostly unrelated origins, indicating a genetic relationship among peanut-nodulating populations isolated from different peanut cropping systems. Furthermore, sequence analysis of the 16S rRNA gene demonstrated identity of isolates with strains of the genus Bradyrhizobium, and phylogenetic association among rhizobial populations from sites with different peanut cropping histories. Diversity indexes estimated for RFLP genotypes showed that populations obtained from PH soils were less diverse than those from NPH soils, suggesting that the presence of the legume results in selection of particular rhizobial strains. Higher polymorphism and diversity were revealed by enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis. Peanut strains clustered at very low levels of similarity (55%). Populations of different origins clustered together, although at elevated genetic distance. ERIC-PCR results indicated the presence of different rhizobial populations in soils of Córdoba province.
Fil: Nievas, Fiorela Lujan. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Bogino, Pablo Cesar. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Nocelli, Natalia Estefanía. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Giordano, Walter Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
description Peanut (Arachis hypogaea) is one of the most important crops of Córdoba province, Argentina; however, knowledge of the genetic diversity of peanut-nodulating rhizobial populations is fragmentary. In view of the importance of information on native rhizobial populations in relation to improved agricultural management practices, we performed genetic characterization of rhizobia isolated from root nodules of plants grown on soils with previous peanut cropping history (PH: Río Cuarto and Cabrera sites) vs. soils with no previous peanut cropping history (NPH: La Aguada and Chaján sites). Ten different 16S rRNA restriction fragment length polymorphism (RFLP) genotypes were obtained through combination of restriction patterns obtained with four endonucleases. Cluster analysis of genotypes gave two major groups at 80% similarity. Each group contained strains from mostly unrelated origins, indicating a genetic relationship among peanut-nodulating populations isolated from different peanut cropping systems. Furthermore, sequence analysis of the 16S rRNA gene demonstrated identity of isolates with strains of the genus Bradyrhizobium, and phylogenetic association among rhizobial populations from sites with different peanut cropping histories. Diversity indexes estimated for RFLP genotypes showed that populations obtained from PH soils were less diverse than those from NPH soils, suggesting that the presence of the legume results in selection of particular rhizobial strains. Higher polymorphism and diversity were revealed by enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis. Peanut strains clustered at very low levels of similarity (55%). Populations of different origins clustered together, although at elevated genetic distance. ERIC-PCR results indicated the presence of different rhizobial populations in soils of Córdoba province.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/198533
Nievas, Fiorela Lujan; Bogino, Pablo Cesar; Nocelli, Natalia Estefanía; Giordano, Walter Fabian; Genotypic analysis of isolated peanut-nodulating rhizobial strains reveals differences among populations obtained from soils with different cropping histories; Elsevier Science; Applied Soil Ecology; 53; 1; 2-2012; 74-82
0929-1393
CONICET Digital
CONICET
url http://hdl.handle.net/11336/198533
identifier_str_mv Nievas, Fiorela Lujan; Bogino, Pablo Cesar; Nocelli, Natalia Estefanía; Giordano, Walter Fabian; Genotypic analysis of isolated peanut-nodulating rhizobial strains reveals differences among populations obtained from soils with different cropping histories; Elsevier Science; Applied Soil Ecology; 53; 1; 2-2012; 74-82
0929-1393
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0929139311003155
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.apsoil.2011.11.010
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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