Targeting of insect epicuticular lipids by entomopathogenic fungi: hydrocarbon oxidation within the context of a host-pathogen interaction

Autores
Pedrini, Nicolás; Ortiz Urquiza, Almudena; Huarte Bonnet, Carla; Zhang, Shizhu; Keyhani, Nemat O.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Broad host range entomopathogenic fungi such as Beauveria bassiana attack insect hosts via attachment to cuticular substrata and the production of enzymes for the degradation and penetration of insect cuticle. The outermost epicuticular layer consists of a complex mixture of non-polar lipids including hydrocarbons, fatty acids, and wax esters. Long chain hydrocarbons are major components of the outer waxy layer of diverse insect species, where they serve to protect against desiccation and microbial parasites, and as recognition molecules or as a platform for semiochemicals. Insect pathogenic fungi have evolved mechanisms for overcoming this barrier, likely with sets of lipid degrading enzymes with overlapping substrate specificities. Alkanes and fatty acids are substrates for a specific subset of fungal cytochrome P450 monooxygenases involved in insect hydrocarbon degradation. These enzymes activate alkanes by terminal oxidation to alcohols, which are further oxidized by alcohol and aldehyde dehydrogenases, whose products can enter β-oxidation pathways. B. bassiana contains at least 83 genes coding for cytochrome P450s (CYP), a subset of which are involved in hydrocarbon oxidation, and several of which represent new CYP subfamilies/families. Expression data indicated differential induction by alkanes and insect lipids and four CYP proteins have been partially characterized after heterologous expression in yeast. Gene knockouts revealed a phenotype for only one (cyp52X1) out of six genes examined to date. CYP52X1 oxidizes long chain fatty acids and participates in the degradation of specific epicuticular lipid components needed for breaching the insect waxy layer. Examining the hydrocarbon oxidizing CYP repertoire of pathogens involved in insect epicuticle degradation can lead to the characterization of enzymes with novel substrate specificities. Pathogen targeting may also represent an important co-evolutionary process regarding insect cuticular hydrocarbon synthesis.
Fil: Pedrini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Ortiz Urquiza, Almudena. University of Florida; Estados Unidos
Fil: Huarte Bonnet, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Zhang, Shizhu. University of Florida; Estados Unidos. Nanjing Normal University; China
Fil: Keyhani, Nemat O.. University of Florida; Estados Unidos
Materia
B. BASSIANA
ENTOMOPATHOGENIC FUNGI
EPICUTICLE
HYDROCARBON DEGRADATION
CYTOCHROME P450
HOST-PATHOGEN COEVOLUTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24118

id CONICETDig_ed74c904ab35afccbad407af5c776560
oai_identifier_str oai:ri.conicet.gov.ar:11336/24118
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Targeting of insect epicuticular lipids by entomopathogenic fungi: hydrocarbon oxidation within the context of a host-pathogen interactionPedrini, NicolásOrtiz Urquiza, AlmudenaHuarte Bonnet, CarlaZhang, ShizhuKeyhani, Nemat O.B. BASSIANAENTOMOPATHOGENIC FUNGIEPICUTICLEHYDROCARBON DEGRADATIONCYTOCHROME P450HOST-PATHOGEN COEVOLUTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Broad host range entomopathogenic fungi such as Beauveria bassiana attack insect hosts via attachment to cuticular substrata and the production of enzymes for the degradation and penetration of insect cuticle. The outermost epicuticular layer consists of a complex mixture of non-polar lipids including hydrocarbons, fatty acids, and wax esters. Long chain hydrocarbons are major components of the outer waxy layer of diverse insect species, where they serve to protect against desiccation and microbial parasites, and as recognition molecules or as a platform for semiochemicals. Insect pathogenic fungi have evolved mechanisms for overcoming this barrier, likely with sets of lipid degrading enzymes with overlapping substrate specificities. Alkanes and fatty acids are substrates for a specific subset of fungal cytochrome P450 monooxygenases involved in insect hydrocarbon degradation. These enzymes activate alkanes by terminal oxidation to alcohols, which are further oxidized by alcohol and aldehyde dehydrogenases, whose products can enter β-oxidation pathways. B. bassiana contains at least 83 genes coding for cytochrome P450s (CYP), a subset of which are involved in hydrocarbon oxidation, and several of which represent new CYP subfamilies/families. Expression data indicated differential induction by alkanes and insect lipids and four CYP proteins have been partially characterized after heterologous expression in yeast. Gene knockouts revealed a phenotype for only one (cyp52X1) out of six genes examined to date. CYP52X1 oxidizes long chain fatty acids and participates in the degradation of specific epicuticular lipid components needed for breaching the insect waxy layer. Examining the hydrocarbon oxidizing CYP repertoire of pathogens involved in insect epicuticle degradation can lead to the characterization of enzymes with novel substrate specificities. Pathogen targeting may also represent an important co-evolutionary process regarding insect cuticular hydrocarbon synthesis.Fil: Pedrini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Ortiz Urquiza, Almudena. University of Florida; Estados UnidosFil: Huarte Bonnet, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Zhang, Shizhu. University of Florida; Estados Unidos. Nanjing Normal University; ChinaFil: Keyhani, Nemat O.. University of Florida; Estados UnidosFrontiers Media S. A2013-02-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24118Pedrini, Nicolás; Ortiz Urquiza, Almudena; Huarte Bonnet, Carla; Zhang, Shizhu; Keyhani, Nemat O.; Targeting of insect epicuticular lipids by entomopathogenic fungi: hydrocarbon oxidation within the context of a host-pathogen interaction; Frontiers Media S. A; Frontiers in Microbiology; 4; 15-2-2013; 1-181664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fmicb.2013.00024/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2013.00024info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:54:15Zoai:ri.conicet.gov.ar:11336/24118instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:54:15.588CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Targeting of insect epicuticular lipids by entomopathogenic fungi: hydrocarbon oxidation within the context of a host-pathogen interaction
title Targeting of insect epicuticular lipids by entomopathogenic fungi: hydrocarbon oxidation within the context of a host-pathogen interaction
spellingShingle Targeting of insect epicuticular lipids by entomopathogenic fungi: hydrocarbon oxidation within the context of a host-pathogen interaction
Pedrini, Nicolás
B. BASSIANA
ENTOMOPATHOGENIC FUNGI
EPICUTICLE
HYDROCARBON DEGRADATION
CYTOCHROME P450
HOST-PATHOGEN COEVOLUTION
title_short Targeting of insect epicuticular lipids by entomopathogenic fungi: hydrocarbon oxidation within the context of a host-pathogen interaction
title_full Targeting of insect epicuticular lipids by entomopathogenic fungi: hydrocarbon oxidation within the context of a host-pathogen interaction
title_fullStr Targeting of insect epicuticular lipids by entomopathogenic fungi: hydrocarbon oxidation within the context of a host-pathogen interaction
title_full_unstemmed Targeting of insect epicuticular lipids by entomopathogenic fungi: hydrocarbon oxidation within the context of a host-pathogen interaction
title_sort Targeting of insect epicuticular lipids by entomopathogenic fungi: hydrocarbon oxidation within the context of a host-pathogen interaction
dc.creator.none.fl_str_mv Pedrini, Nicolás
Ortiz Urquiza, Almudena
Huarte Bonnet, Carla
Zhang, Shizhu
Keyhani, Nemat O.
author Pedrini, Nicolás
author_facet Pedrini, Nicolás
Ortiz Urquiza, Almudena
Huarte Bonnet, Carla
Zhang, Shizhu
Keyhani, Nemat O.
author_role author
author2 Ortiz Urquiza, Almudena
Huarte Bonnet, Carla
Zhang, Shizhu
Keyhani, Nemat O.
author2_role author
author
author
author
dc.subject.none.fl_str_mv B. BASSIANA
ENTOMOPATHOGENIC FUNGI
EPICUTICLE
HYDROCARBON DEGRADATION
CYTOCHROME P450
HOST-PATHOGEN COEVOLUTION
topic B. BASSIANA
ENTOMOPATHOGENIC FUNGI
EPICUTICLE
HYDROCARBON DEGRADATION
CYTOCHROME P450
HOST-PATHOGEN COEVOLUTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Broad host range entomopathogenic fungi such as Beauveria bassiana attack insect hosts via attachment to cuticular substrata and the production of enzymes for the degradation and penetration of insect cuticle. The outermost epicuticular layer consists of a complex mixture of non-polar lipids including hydrocarbons, fatty acids, and wax esters. Long chain hydrocarbons are major components of the outer waxy layer of diverse insect species, where they serve to protect against desiccation and microbial parasites, and as recognition molecules or as a platform for semiochemicals. Insect pathogenic fungi have evolved mechanisms for overcoming this barrier, likely with sets of lipid degrading enzymes with overlapping substrate specificities. Alkanes and fatty acids are substrates for a specific subset of fungal cytochrome P450 monooxygenases involved in insect hydrocarbon degradation. These enzymes activate alkanes by terminal oxidation to alcohols, which are further oxidized by alcohol and aldehyde dehydrogenases, whose products can enter β-oxidation pathways. B. bassiana contains at least 83 genes coding for cytochrome P450s (CYP), a subset of which are involved in hydrocarbon oxidation, and several of which represent new CYP subfamilies/families. Expression data indicated differential induction by alkanes and insect lipids and four CYP proteins have been partially characterized after heterologous expression in yeast. Gene knockouts revealed a phenotype for only one (cyp52X1) out of six genes examined to date. CYP52X1 oxidizes long chain fatty acids and participates in the degradation of specific epicuticular lipid components needed for breaching the insect waxy layer. Examining the hydrocarbon oxidizing CYP repertoire of pathogens involved in insect epicuticle degradation can lead to the characterization of enzymes with novel substrate specificities. Pathogen targeting may also represent an important co-evolutionary process regarding insect cuticular hydrocarbon synthesis.
Fil: Pedrini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Ortiz Urquiza, Almudena. University of Florida; Estados Unidos
Fil: Huarte Bonnet, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Zhang, Shizhu. University of Florida; Estados Unidos. Nanjing Normal University; China
Fil: Keyhani, Nemat O.. University of Florida; Estados Unidos
description Broad host range entomopathogenic fungi such as Beauveria bassiana attack insect hosts via attachment to cuticular substrata and the production of enzymes for the degradation and penetration of insect cuticle. The outermost epicuticular layer consists of a complex mixture of non-polar lipids including hydrocarbons, fatty acids, and wax esters. Long chain hydrocarbons are major components of the outer waxy layer of diverse insect species, where they serve to protect against desiccation and microbial parasites, and as recognition molecules or as a platform for semiochemicals. Insect pathogenic fungi have evolved mechanisms for overcoming this barrier, likely with sets of lipid degrading enzymes with overlapping substrate specificities. Alkanes and fatty acids are substrates for a specific subset of fungal cytochrome P450 monooxygenases involved in insect hydrocarbon degradation. These enzymes activate alkanes by terminal oxidation to alcohols, which are further oxidized by alcohol and aldehyde dehydrogenases, whose products can enter β-oxidation pathways. B. bassiana contains at least 83 genes coding for cytochrome P450s (CYP), a subset of which are involved in hydrocarbon oxidation, and several of which represent new CYP subfamilies/families. Expression data indicated differential induction by alkanes and insect lipids and four CYP proteins have been partially characterized after heterologous expression in yeast. Gene knockouts revealed a phenotype for only one (cyp52X1) out of six genes examined to date. CYP52X1 oxidizes long chain fatty acids and participates in the degradation of specific epicuticular lipid components needed for breaching the insect waxy layer. Examining the hydrocarbon oxidizing CYP repertoire of pathogens involved in insect epicuticle degradation can lead to the characterization of enzymes with novel substrate specificities. Pathogen targeting may also represent an important co-evolutionary process regarding insect cuticular hydrocarbon synthesis.
publishDate 2013
dc.date.none.fl_str_mv 2013-02-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24118
Pedrini, Nicolás; Ortiz Urquiza, Almudena; Huarte Bonnet, Carla; Zhang, Shizhu; Keyhani, Nemat O.; Targeting of insect epicuticular lipids by entomopathogenic fungi: hydrocarbon oxidation within the context of a host-pathogen interaction; Frontiers Media S. A; Frontiers in Microbiology; 4; 15-2-2013; 1-18
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24118
identifier_str_mv Pedrini, Nicolás; Ortiz Urquiza, Almudena; Huarte Bonnet, Carla; Zhang, Shizhu; Keyhani, Nemat O.; Targeting of insect epicuticular lipids by entomopathogenic fungi: hydrocarbon oxidation within the context of a host-pathogen interaction; Frontiers Media S. A; Frontiers in Microbiology; 4; 15-2-2013; 1-18
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fmicb.2013.00024/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2013.00024
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S. A
publisher.none.fl_str_mv Frontiers Media S. A
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613649693409280
score 13.070432