Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes

Autores
Huarte Bonnet, Carla; Kumar, Suresh; Saparrat, Mario Carlos Nazareno; Girotti, Juan Roberto; Santana, Marianela; Hallsworth, John E.; Pedrini, Nicolás
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Several filamentous fungi are able to concomitantly assimilate both aliphatic and polycyclic aromatic hydrocarbons that are the biogenic by-products of some industrial processes. Cytochrome P450 monooxygenases catalyze the first oxidation reaction for both types of substrate. Among the cytochrome P450 (CYP) genes, the family CYP52 is implicated in the first hydroxylation step in alkane-assimilation processes, while genes belonging to the family CYP53 have been linked with oxidation of aromatic hydrocarbons. Here, we perform a comparative analysis of CYP genes belonging to clans CYP52 and CYP53 in Aspergillus niger, Beauveria bassiana, Metarhizium robertsii (formerly M. anisopliae var. anisopliae), and Penicillium chrysogenum. These species were able to assimilate n-hexadecane, n-octacosane, and phenanthrene, exhibiting a species-dependent modification in pH of the nutrient medium during this process. Modeling of the molecular docking of the hydrocarbons to the cytochrome P450 active site revealed that both phenanthrene and n-octacosane are energetically favored as substrates for the enzymes codified by genes belonging to both CYP52 and CYP53 clans, and thus appear to be involved in this oxidation step. Analyses of gene expression revealed that CYP53 members were significantly induced by phenanthrene in all species studied, but only CYP52X1 and CYP53A11 from B. bassiana were highly induced with n-alkanes. These findings suggest that the set of P450 enzymes involved in hydrocarbon assimilation by fungi is dependent on phylogeny and reveal distinct substrate and expression specificities.
Instituto de Investigaciones Bioquímicas de La Plata
Instituto de Fisiología Vegetal
Facultad de Ciencias Agrarias y Forestales
Instituto de Botánica "Dr. Carlos Spegazzini"
Materia
Biología
Ciencias Naturales
Aspergillus niger
Beauveria bassiana
Entomopathogenic fungi
Hydrocarbon degradation
Metarhizium anisopliae
Penicillium chrysogenum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/141006
Acceder
id SEDICI_907e9ca2d47a7bed5cc0a0cbeb85aea1
oai_identifier_str oai:sedici.unlp.edu.ar:10915/141006
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 GenesHuarte Bonnet, CarlaKumar, SureshSaparrat, Mario Carlos NazarenoGirotti, Juan RobertoSantana, MarianelaHallsworth, John E.Pedrini, NicolásBiologíaCiencias NaturalesAspergillus nigerBeauveria bassianaEntomopathogenic fungiHydrocarbon degradationMetarhizium anisopliaePenicillium chrysogenumSeveral filamentous fungi are able to concomitantly assimilate both aliphatic and polycyclic aromatic hydrocarbons that are the biogenic by-products of some industrial processes. Cytochrome P450 monooxygenases catalyze the first oxidation reaction for both types of substrate. Among the cytochrome P450 (CYP) genes, the family CYP52 is implicated in the first hydroxylation step in alkane-assimilation processes, while genes belonging to the family CYP53 have been linked with oxidation of aromatic hydrocarbons. Here, we perform a comparative analysis of CYP genes belonging to clans CYP52 and CYP53 in Aspergillus niger, Beauveria bassiana, Metarhizium robertsii (formerly M. anisopliae var. anisopliae), and Penicillium chrysogenum. These species were able to assimilate n-hexadecane, n-octacosane, and phenanthrene, exhibiting a species-dependent modification in pH of the nutrient medium during this process. Modeling of the molecular docking of the hydrocarbons to the cytochrome P450 active site revealed that both phenanthrene and n-octacosane are energetically favored as substrates for the enzymes codified by genes belonging to both CYP52 and CYP53 clans, and thus appear to be involved in this oxidation step. Analyses of gene expression revealed that CYP53 members were significantly induced by phenanthrene in all species studied, but only CYP52X1 and CYP53A11 from B. bassiana were highly induced with n-alkanes. These findings suggest that the set of P450 enzymes involved in hydrocarbon assimilation by fungi is dependent on phylogeny and reveal distinct substrate and expression specificities.Instituto de Investigaciones Bioquímicas de La PlataInstituto de Fisiología VegetalFacultad de Ciencias Agrarias y ForestalesInstituto de Botánica "Dr. Carlos Spegazzini"2017-09-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1047-1060http://sedici.unlp.edu.ar/handle/10915/141006enginfo:eu-repo/semantics/altIdentifier/issn/1559-0291info:eu-repo/semantics/altIdentifier/issn/0273-2289info:eu-repo/semantics/altIdentifier/doi/10.1007/s12010-017-2608-zinfo:eu-repo/semantics/altIdentifier/pmid/28942502info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:04:19Zoai:sedici.unlp.edu.ar:10915/141006Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:04:19.465SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
title Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
spellingShingle Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
Huarte Bonnet, Carla
Biología
Ciencias Naturales
Aspergillus niger
Beauveria bassiana
Entomopathogenic fungi
Hydrocarbon degradation
Metarhizium anisopliae
Penicillium chrysogenum
title_short Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
title_full Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
title_fullStr Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
title_full_unstemmed Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
title_sort Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi : Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
dc.creator.none.fl_str_mv Huarte Bonnet, Carla
Kumar, Suresh
Saparrat, Mario Carlos Nazareno
Girotti, Juan Roberto
Santana, Marianela
Hallsworth, John E.
Pedrini, Nicolás
author Huarte Bonnet, Carla
author_facet Huarte Bonnet, Carla
Kumar, Suresh
Saparrat, Mario Carlos Nazareno
Girotti, Juan Roberto
Santana, Marianela
Hallsworth, John E.
Pedrini, Nicolás
author_role author
author2 Kumar, Suresh
Saparrat, Mario Carlos Nazareno
Girotti, Juan Roberto
Santana, Marianela
Hallsworth, John E.
Pedrini, Nicolás
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Ciencias Naturales
Aspergillus niger
Beauveria bassiana
Entomopathogenic fungi
Hydrocarbon degradation
Metarhizium anisopliae
Penicillium chrysogenum
topic Biología
Ciencias Naturales
Aspergillus niger
Beauveria bassiana
Entomopathogenic fungi
Hydrocarbon degradation
Metarhizium anisopliae
Penicillium chrysogenum
dc.description.none.fl_txt_mv Several filamentous fungi are able to concomitantly assimilate both aliphatic and polycyclic aromatic hydrocarbons that are the biogenic by-products of some industrial processes. Cytochrome P450 monooxygenases catalyze the first oxidation reaction for both types of substrate. Among the cytochrome P450 (CYP) genes, the family CYP52 is implicated in the first hydroxylation step in alkane-assimilation processes, while genes belonging to the family CYP53 have been linked with oxidation of aromatic hydrocarbons. Here, we perform a comparative analysis of CYP genes belonging to clans CYP52 and CYP53 in Aspergillus niger, Beauveria bassiana, Metarhizium robertsii (formerly M. anisopliae var. anisopliae), and Penicillium chrysogenum. These species were able to assimilate n-hexadecane, n-octacosane, and phenanthrene, exhibiting a species-dependent modification in pH of the nutrient medium during this process. Modeling of the molecular docking of the hydrocarbons to the cytochrome P450 active site revealed that both phenanthrene and n-octacosane are energetically favored as substrates for the enzymes codified by genes belonging to both CYP52 and CYP53 clans, and thus appear to be involved in this oxidation step. Analyses of gene expression revealed that CYP53 members were significantly induced by phenanthrene in all species studied, but only CYP52X1 and CYP53A11 from B. bassiana were highly induced with n-alkanes. These findings suggest that the set of P450 enzymes involved in hydrocarbon assimilation by fungi is dependent on phylogeny and reveal distinct substrate and expression specificities.
Instituto de Investigaciones Bioquímicas de La Plata
Instituto de Fisiología Vegetal
Facultad de Ciencias Agrarias y Forestales
Instituto de Botánica "Dr. Carlos Spegazzini"
description Several filamentous fungi are able to concomitantly assimilate both aliphatic and polycyclic aromatic hydrocarbons that are the biogenic by-products of some industrial processes. Cytochrome P450 monooxygenases catalyze the first oxidation reaction for both types of substrate. Among the cytochrome P450 (CYP) genes, the family CYP52 is implicated in the first hydroxylation step in alkane-assimilation processes, while genes belonging to the family CYP53 have been linked with oxidation of aromatic hydrocarbons. Here, we perform a comparative analysis of CYP genes belonging to clans CYP52 and CYP53 in Aspergillus niger, Beauveria bassiana, Metarhizium robertsii (formerly M. anisopliae var. anisopliae), and Penicillium chrysogenum. These species were able to assimilate n-hexadecane, n-octacosane, and phenanthrene, exhibiting a species-dependent modification in pH of the nutrient medium during this process. Modeling of the molecular docking of the hydrocarbons to the cytochrome P450 active site revealed that both phenanthrene and n-octacosane are energetically favored as substrates for the enzymes codified by genes belonging to both CYP52 and CYP53 clans, and thus appear to be involved in this oxidation step. Analyses of gene expression revealed that CYP53 members were significantly induced by phenanthrene in all species studied, but only CYP52X1 and CYP53A11 from B. bassiana were highly induced with n-alkanes. These findings suggest that the set of P450 enzymes involved in hydrocarbon assimilation by fungi is dependent on phylogeny and reveal distinct substrate and expression specificities.
publishDate 2017
dc.date.none.fl_str_mv 2017-09-23
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/141006
url http://sedici.unlp.edu.ar/handle/10915/141006
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1559-0291
info:eu-repo/semantics/altIdentifier/issn/0273-2289
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12010-017-2608-z
info:eu-repo/semantics/altIdentifier/pmid/28942502
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
1047-1060
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1842260541938073600
score 13.13397

Publicaciones similares