δ conversion parameter between pH scales (WSpH and SSPH) in acetonitrile/water mixtures at various compositions and temperatures

Autores
Gagliardi, Leonardo Gabriel; Castells, Cecilia Beatriz Marta; Ràfols, Clara; Rosés, Martí; Bosch, Elisabeth
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The SSpH in acetonitrile/water mixtures at different temperatures cannot be directly measured because of the lack of calibration buffers in these hydroorganic media at most temperatures different from 25°C. In this paper, the δ parameter has been determined for acetonitrile/water mixtures from 0 up to 90% acetonitrile at different temperatures from 15 to 60°C, and the values were fitted to a very simple simultaneous function of composition and temperature. The δ values allow conversion of the WSpH scale (pH measured in acetonitrile/water with electrodes calibrated in water) to the S SpH scale (pH measured in acetonitrile/water with electrodes calibrated in the same acetonitrile/water mixture). The practical determination of WSpH is direct because the calibration of the electrodes is carried out with commercial aqueous standard buffers. Thus, the SSpH value of any buffered acetonitrile/water mobile phase used in reversed-phase liquid chromatography, which is directly related to the ionized fraction of analyte and, therefore, to its average retention, can be easily known at any temperature from the measured WSpH and the corresponding δ value.
Fil: Gagliardi, Leonardo Gabriel. Universidad de Barcelona; España. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
Fil: Castells, Cecilia Beatriz Marta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Ràfols, Clara. Universidad de Barcelona; España
Fil: Rosés, Martí. Universidad de Barcelona; España
Fil: Bosch, Elisabeth. Universidad de Barcelona; España
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95504
Acceder
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network_name_str CONICET Digital (CONICET)
spelling δ conversion parameter between pH scales (WSpH and SSPH) in acetonitrile/water mixtures at various compositions and temperaturesGagliardi, Leonardo GabrielCastells, Cecilia Beatriz MartaRàfols, ClaraRosés, MartíBosch, Elisabethhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The SSpH in acetonitrile/water mixtures at different temperatures cannot be directly measured because of the lack of calibration buffers in these hydroorganic media at most temperatures different from 25°C. In this paper, the δ parameter has been determined for acetonitrile/water mixtures from 0 up to 90% acetonitrile at different temperatures from 15 to 60°C, and the values were fitted to a very simple simultaneous function of composition and temperature. The δ values allow conversion of the WSpH scale (pH measured in acetonitrile/water with electrodes calibrated in water) to the S SpH scale (pH measured in acetonitrile/water with electrodes calibrated in the same acetonitrile/water mixture). The practical determination of WSpH is direct because the calibration of the electrodes is carried out with commercial aqueous standard buffers. Thus, the SSpH value of any buffered acetonitrile/water mobile phase used in reversed-phase liquid chromatography, which is directly related to the ionized fraction of analyte and, therefore, to its average retention, can be easily known at any temperature from the measured WSpH and the corresponding δ value.Fil: Gagliardi, Leonardo Gabriel. Universidad de Barcelona; España. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; ArgentinaFil: Castells, Cecilia Beatriz Marta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata; ArgentinaFil: Ràfols, Clara. Universidad de Barcelona; EspañaFil: Rosés, Martí. Universidad de Barcelona; EspañaFil: Bosch, Elisabeth. Universidad de Barcelona; EspañaAmerican Chemical Society2007-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95504Gagliardi, Leonardo Gabriel; Castells, Cecilia Beatriz Marta; Ràfols, Clara; Rosés, Martí; Bosch, Elisabeth; δ conversion parameter between pH scales (WSpH and SSPH) in acetonitrile/water mixtures at various compositions and temperatures; American Chemical Society; Analytical Chemistry; 79; 8; 4-2007; 3180-31870003-2700CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/ac062372hinfo:eu-repo/semantics/altIdentifier/doi/10.1021/ac062372hinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-06-10T09:58:39Zoai:ri.conicet.gov.ar:11336/95504instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-06-10 09:58:39.464CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv δ conversion parameter between pH scales (WSpH and SSPH) in acetonitrile/water mixtures at various compositions and temperatures
title δ conversion parameter between pH scales (WSpH and SSPH) in acetonitrile/water mixtures at various compositions and temperatures
spellingShingle δ conversion parameter between pH scales (WSpH and SSPH) in acetonitrile/water mixtures at various compositions and temperatures
Gagliardi, Leonardo Gabriel
title_short δ conversion parameter between pH scales (WSpH and SSPH) in acetonitrile/water mixtures at various compositions and temperatures
title_full δ conversion parameter between pH scales (WSpH and SSPH) in acetonitrile/water mixtures at various compositions and temperatures
title_fullStr δ conversion parameter between pH scales (WSpH and SSPH) in acetonitrile/water mixtures at various compositions and temperatures
title_full_unstemmed δ conversion parameter between pH scales (WSpH and SSPH) in acetonitrile/water mixtures at various compositions and temperatures
title_sort δ conversion parameter between pH scales (WSpH and SSPH) in acetonitrile/water mixtures at various compositions and temperatures
dc.creator.none.fl_str_mv Gagliardi, Leonardo Gabriel
Castells, Cecilia Beatriz Marta
Ràfols, Clara
Rosés, Martí
Bosch, Elisabeth
author Gagliardi, Leonardo Gabriel
author_facet Gagliardi, Leonardo Gabriel
Castells, Cecilia Beatriz Marta
Ràfols, Clara
Rosés, Martí
Bosch, Elisabeth
author_role author
author2 Castells, Cecilia Beatriz Marta
Ràfols, Clara
Rosés, Martí
Bosch, Elisabeth
author2_role author
author
author
author
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The SSpH in acetonitrile/water mixtures at different temperatures cannot be directly measured because of the lack of calibration buffers in these hydroorganic media at most temperatures different from 25°C. In this paper, the δ parameter has been determined for acetonitrile/water mixtures from 0 up to 90% acetonitrile at different temperatures from 15 to 60°C, and the values were fitted to a very simple simultaneous function of composition and temperature. The δ values allow conversion of the WSpH scale (pH measured in acetonitrile/water with electrodes calibrated in water) to the S SpH scale (pH measured in acetonitrile/water with electrodes calibrated in the same acetonitrile/water mixture). The practical determination of WSpH is direct because the calibration of the electrodes is carried out with commercial aqueous standard buffers. Thus, the SSpH value of any buffered acetonitrile/water mobile phase used in reversed-phase liquid chromatography, which is directly related to the ionized fraction of analyte and, therefore, to its average retention, can be easily known at any temperature from the measured WSpH and the corresponding δ value.
Fil: Gagliardi, Leonardo Gabriel. Universidad de Barcelona; España. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
Fil: Castells, Cecilia Beatriz Marta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Ràfols, Clara. Universidad de Barcelona; España
Fil: Rosés, Martí. Universidad de Barcelona; España
Fil: Bosch, Elisabeth. Universidad de Barcelona; España
description The SSpH in acetonitrile/water mixtures at different temperatures cannot be directly measured because of the lack of calibration buffers in these hydroorganic media at most temperatures different from 25°C. In this paper, the δ parameter has been determined for acetonitrile/water mixtures from 0 up to 90% acetonitrile at different temperatures from 15 to 60°C, and the values were fitted to a very simple simultaneous function of composition and temperature. The δ values allow conversion of the WSpH scale (pH measured in acetonitrile/water with electrodes calibrated in water) to the S SpH scale (pH measured in acetonitrile/water with electrodes calibrated in the same acetonitrile/water mixture). The practical determination of WSpH is direct because the calibration of the electrodes is carried out with commercial aqueous standard buffers. Thus, the SSpH value of any buffered acetonitrile/water mobile phase used in reversed-phase liquid chromatography, which is directly related to the ionized fraction of analyte and, therefore, to its average retention, can be easily known at any temperature from the measured WSpH and the corresponding δ value.
publishDate 2007
dc.date.none.fl_str_mv 2007-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95504
Gagliardi, Leonardo Gabriel; Castells, Cecilia Beatriz Marta; Ràfols, Clara; Rosés, Martí; Bosch, Elisabeth; δ conversion parameter between pH scales (WSpH and SSPH) in acetonitrile/water mixtures at various compositions and temperatures; American Chemical Society; Analytical Chemistry; 79; 8; 4-2007; 3180-3187
0003-2700
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95504
identifier_str_mv Gagliardi, Leonardo Gabriel; Castells, Cecilia Beatriz Marta; Ràfols, Clara; Rosés, Martí; Bosch, Elisabeth; δ conversion parameter between pH scales (WSpH and SSPH) in acetonitrile/water mixtures at various compositions and temperatures; American Chemical Society; Analytical Chemistry; 79; 8; 4-2007; 3180-3187
0003-2700
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/ac062372h
info:eu-repo/semantics/altIdentifier/doi/10.1021/ac062372h
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1867629680582983680
score 12.98848

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