An α-glucan elicitor from the cell wall of a biocontrol binucleate Rhizoctonia isolate

Autores
Wolski, Erika Alejandra; Lima, Carlos Eduardo; Agusti, Rosalia; Daleo, Gustavo Raul; Andreu, Adriana Balbina; de Lederkremer, Rosa M.
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Binucleate Rhizoctonia (BNR) isolate (232-C6) is an effective biocontrol agent for protection of potato from Rhizoctonia canker, a disease caused by Rhizoctonia solani. Production of hydrolytic enzymes is one of the best known inducible defense responses following microbial infection. We isolated and characterized a cell wall α-glucan from BNR, which induces β-1,3 glucanase activities in potato sprouts, the primary site of infection by R. solani. An autoclaving method, previously reported for isolation of oligosaccharide elicitors was used, and the glucan purified by chromatographic techniques. Maximal induction of β-1,3 glucanase activity in potato sprouts was obtained with 250 μg of the α-glucan elicitor after 6 days from inoculation time. Both, BNR mycelium and the α-glucan produced a similar kinetic response of β-1,3 glucanase. However, the α-glucan did not induce phytoalexin accumulation, previously correlated with the defense response. Uronic acids (∼10% with respect to total neutral sugars) were determined and identified as glucuronic acid by high-pH anion-exchange chromatography. Methylation analysis showed that the glucan consists of (1→3) and (1→4)-linked glucose units with preponderance of the first ones. Some of the (1→4) linkages were branched at position 6. The glucan was partially degraded with amyloglucosidase. This, together with the NMR spectra data and the high optical rotation of the original (+195°) and degraded glucans (+175°) proved the α configuration. Further methylation of the amyloglucosidase degraded glucans indicated that they consist of (1→3)-linked glucoses. The present study is the first report on the isolation and characterization of an α-glucan from Rhizoctonia, that may be important as a biocontrol factor.
Fil: Wolski, Erika Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Lima, Carlos Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Agusti, Rosalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Daleo, Gustavo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Andreu, Adriana Balbina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: de Lederkremer, Rosa M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Materia
RHIZOCTONIA (BNR)
POTATO
232-C6
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/240848
Acceder
id CONICETDig_ec39df6758c11bf94ff46fa1862075e3
oai_identifier_str oai:ri.conicet.gov.ar:11336/240848
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An α-glucan elicitor from the cell wall of a biocontrol binucleate Rhizoctonia isolateWolski, Erika AlejandraLima, Carlos EduardoAgusti, RosaliaDaleo, Gustavo RaulAndreu, Adriana Balbinade Lederkremer, Rosa M.RHIZOCTONIA (BNR)POTATO232-C6https://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Binucleate Rhizoctonia (BNR) isolate (232-C6) is an effective biocontrol agent for protection of potato from Rhizoctonia canker, a disease caused by Rhizoctonia solani. Production of hydrolytic enzymes is one of the best known inducible defense responses following microbial infection. We isolated and characterized a cell wall α-glucan from BNR, which induces β-1,3 glucanase activities in potato sprouts, the primary site of infection by R. solani. An autoclaving method, previously reported for isolation of oligosaccharide elicitors was used, and the glucan purified by chromatographic techniques. Maximal induction of β-1,3 glucanase activity in potato sprouts was obtained with 250 μg of the α-glucan elicitor after 6 days from inoculation time. Both, BNR mycelium and the α-glucan produced a similar kinetic response of β-1,3 glucanase. However, the α-glucan did not induce phytoalexin accumulation, previously correlated with the defense response. Uronic acids (∼10% with respect to total neutral sugars) were determined and identified as glucuronic acid by high-pH anion-exchange chromatography. Methylation analysis showed that the glucan consists of (1→3) and (1→4)-linked glucose units with preponderance of the first ones. Some of the (1→4) linkages were branched at position 6. The glucan was partially degraded with amyloglucosidase. This, together with the NMR spectra data and the high optical rotation of the original (+195°) and degraded glucans (+175°) proved the α configuration. Further methylation of the amyloglucosidase degraded glucans indicated that they consist of (1→3)-linked glucoses. The present study is the first report on the isolation and characterization of an α-glucan from Rhizoctonia, that may be important as a biocontrol factor.Fil: Wolski, Erika Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Lima, Carlos Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Agusti, Rosalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Daleo, Gustavo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Andreu, Adriana Balbina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: de Lederkremer, Rosa M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaElsevier2005-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/240848Wolski, Erika Alejandra; Lima, Carlos Eduardo; Agusti, Rosalia; Daleo, Gustavo Raul; Andreu, Adriana Balbina; et al.; An α-glucan elicitor from the cell wall of a biocontrol binucleate Rhizoctonia isolate; Elsevier; Carbohydrate Research; 340; 4; 3-2005; 619-6270008-6215CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0008621504005580info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carres.2004.12.020info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:09:50Zoai:ri.conicet.gov.ar:11336/240848instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:09:50.635CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An α-glucan elicitor from the cell wall of a biocontrol binucleate Rhizoctonia isolate
title An α-glucan elicitor from the cell wall of a biocontrol binucleate Rhizoctonia isolate
spellingShingle An α-glucan elicitor from the cell wall of a biocontrol binucleate Rhizoctonia isolate
Wolski, Erika Alejandra
RHIZOCTONIA (BNR)
POTATO
232-C6
title_short An α-glucan elicitor from the cell wall of a biocontrol binucleate Rhizoctonia isolate
title_full An α-glucan elicitor from the cell wall of a biocontrol binucleate Rhizoctonia isolate
title_fullStr An α-glucan elicitor from the cell wall of a biocontrol binucleate Rhizoctonia isolate
title_full_unstemmed An α-glucan elicitor from the cell wall of a biocontrol binucleate Rhizoctonia isolate
title_sort An α-glucan elicitor from the cell wall of a biocontrol binucleate Rhizoctonia isolate
dc.creator.none.fl_str_mv Wolski, Erika Alejandra
Lima, Carlos Eduardo
Agusti, Rosalia
Daleo, Gustavo Raul
Andreu, Adriana Balbina
de Lederkremer, Rosa M.
author Wolski, Erika Alejandra
author_facet Wolski, Erika Alejandra
Lima, Carlos Eduardo
Agusti, Rosalia
Daleo, Gustavo Raul
Andreu, Adriana Balbina
de Lederkremer, Rosa M.
author_role author
author2 Lima, Carlos Eduardo
Agusti, Rosalia
Daleo, Gustavo Raul
Andreu, Adriana Balbina
de Lederkremer, Rosa M.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv RHIZOCTONIA (BNR)
POTATO
232-C6
topic RHIZOCTONIA (BNR)
POTATO
232-C6
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Binucleate Rhizoctonia (BNR) isolate (232-C6) is an effective biocontrol agent for protection of potato from Rhizoctonia canker, a disease caused by Rhizoctonia solani. Production of hydrolytic enzymes is one of the best known inducible defense responses following microbial infection. We isolated and characterized a cell wall α-glucan from BNR, which induces β-1,3 glucanase activities in potato sprouts, the primary site of infection by R. solani. An autoclaving method, previously reported for isolation of oligosaccharide elicitors was used, and the glucan purified by chromatographic techniques. Maximal induction of β-1,3 glucanase activity in potato sprouts was obtained with 250 μg of the α-glucan elicitor after 6 days from inoculation time. Both, BNR mycelium and the α-glucan produced a similar kinetic response of β-1,3 glucanase. However, the α-glucan did not induce phytoalexin accumulation, previously correlated with the defense response. Uronic acids (∼10% with respect to total neutral sugars) were determined and identified as glucuronic acid by high-pH anion-exchange chromatography. Methylation analysis showed that the glucan consists of (1→3) and (1→4)-linked glucose units with preponderance of the first ones. Some of the (1→4) linkages were branched at position 6. The glucan was partially degraded with amyloglucosidase. This, together with the NMR spectra data and the high optical rotation of the original (+195°) and degraded glucans (+175°) proved the α configuration. Further methylation of the amyloglucosidase degraded glucans indicated that they consist of (1→3)-linked glucoses. The present study is the first report on the isolation and characterization of an α-glucan from Rhizoctonia, that may be important as a biocontrol factor.
Fil: Wolski, Erika Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Lima, Carlos Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Agusti, Rosalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Daleo, Gustavo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Andreu, Adriana Balbina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: de Lederkremer, Rosa M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
description Binucleate Rhizoctonia (BNR) isolate (232-C6) is an effective biocontrol agent for protection of potato from Rhizoctonia canker, a disease caused by Rhizoctonia solani. Production of hydrolytic enzymes is one of the best known inducible defense responses following microbial infection. We isolated and characterized a cell wall α-glucan from BNR, which induces β-1,3 glucanase activities in potato sprouts, the primary site of infection by R. solani. An autoclaving method, previously reported for isolation of oligosaccharide elicitors was used, and the glucan purified by chromatographic techniques. Maximal induction of β-1,3 glucanase activity in potato sprouts was obtained with 250 μg of the α-glucan elicitor after 6 days from inoculation time. Both, BNR mycelium and the α-glucan produced a similar kinetic response of β-1,3 glucanase. However, the α-glucan did not induce phytoalexin accumulation, previously correlated with the defense response. Uronic acids (∼10% with respect to total neutral sugars) were determined and identified as glucuronic acid by high-pH anion-exchange chromatography. Methylation analysis showed that the glucan consists of (1→3) and (1→4)-linked glucose units with preponderance of the first ones. Some of the (1→4) linkages were branched at position 6. The glucan was partially degraded with amyloglucosidase. This, together with the NMR spectra data and the high optical rotation of the original (+195°) and degraded glucans (+175°) proved the α configuration. Further methylation of the amyloglucosidase degraded glucans indicated that they consist of (1→3)-linked glucoses. The present study is the first report on the isolation and characterization of an α-glucan from Rhizoctonia, that may be important as a biocontrol factor.
publishDate 2005
dc.date.none.fl_str_mv 2005-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/240848
Wolski, Erika Alejandra; Lima, Carlos Eduardo; Agusti, Rosalia; Daleo, Gustavo Raul; Andreu, Adriana Balbina; et al.; An α-glucan elicitor from the cell wall of a biocontrol binucleate Rhizoctonia isolate; Elsevier; Carbohydrate Research; 340; 4; 3-2005; 619-627
0008-6215
CONICET Digital
CONICET
url http://hdl.handle.net/11336/240848
identifier_str_mv Wolski, Erika Alejandra; Lima, Carlos Eduardo; Agusti, Rosalia; Daleo, Gustavo Raul; Andreu, Adriana Balbina; et al.; An α-glucan elicitor from the cell wall of a biocontrol binucleate Rhizoctonia isolate; Elsevier; Carbohydrate Research; 340; 4; 3-2005; 619-627
0008-6215
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0008621504005580
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carres.2004.12.020
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083246486454272
score 13.22299

Publicaciones similares