Dissemination of an erythromycin-resistant penicillin-nonsusceptible streptococcus pneumoniae Poland6B-20 clone in Argentina

Autores
Bonofiglio, Laura; Regueira, Mabel; Pace, Julio; Corso, Alejandra; García, Ernesto; Mollerach, Marta Eugenia
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Prevalence of serotype 6B penicillin (PEN)-nonsusceptible Streptococcus pneumoniae significantly increased from 15.8% (1993-1997) to 67.3% (1998-2002) (p < 0.001) in Argentina. Serogroup 6 ranks fourth among different capsular types within invasive isolates from Argentinean patients <6 years of age. To evaluate whether the increase in PEN resistance in serotype 6B pneumococci was due to the dissemination of one or more clones, the genetic diversity of 93 S. pneumoniae serotype 6B isolates was analyzed. Five BOX-polymerase chain reaction types were obtained (65.5% isolates) and a group of 15 isolates, representing 41.6% of those having a decreased susceptibility to PEN, were further characterized. The antibiotype of these isolates showed their multiresistance, with 100% of the isolates being resistant to erythromycin, 80% to tetracycline, and 73.3% to trimethoprim-sulfamethoxazole. Of the 15 isolates, 13 belonged to the same pulsed-field gel electrophoresis type and galU cluster and were members of the same clone. The identity of the clone was confirmed in four isolates by multilocus sequence typing. The sequence type found (ST315) corresponds to the Poland6B-20 clone. In summary, BOX-polymerase chain reaction, pulsed-field gel electrophoresis, and galU polymorphism were useful tools to detect the presence of a clone whose identity was confirmed by multilocus sequence typing. The isolates belonging to Poland6B-20 found in this work are described for the first time in Latin America.
Fil: Bonofiglio, Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Regueira, Mabel. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Pace, Julio. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Corso, Alejandra. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: García, Ernesto. Centro de Investigación Biomédica en Red; España
Fil: Mollerach, Marta Eugenia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
S. Pneumoniae
Serotype 6b
Poland6b-20
Galu
Pfge
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67317
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/67317
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dissemination of an erythromycin-resistant penicillin-nonsusceptible streptococcus pneumoniae Poland6B-20 clone in ArgentinaBonofiglio, LauraRegueira, MabelPace, JulioCorso, AlejandraGarcía, ErnestoMollerach, Marta EugeniaS. PneumoniaeSerotype 6bPoland6b-20GaluPfgehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Prevalence of serotype 6B penicillin (PEN)-nonsusceptible Streptococcus pneumoniae significantly increased from 15.8% (1993-1997) to 67.3% (1998-2002) (p < 0.001) in Argentina. Serogroup 6 ranks fourth among different capsular types within invasive isolates from Argentinean patients <6 years of age. To evaluate whether the increase in PEN resistance in serotype 6B pneumococci was due to the dissemination of one or more clones, the genetic diversity of 93 S. pneumoniae serotype 6B isolates was analyzed. Five BOX-polymerase chain reaction types were obtained (65.5% isolates) and a group of 15 isolates, representing 41.6% of those having a decreased susceptibility to PEN, were further characterized. The antibiotype of these isolates showed their multiresistance, with 100% of the isolates being resistant to erythromycin, 80% to tetracycline, and 73.3% to trimethoprim-sulfamethoxazole. Of the 15 isolates, 13 belonged to the same pulsed-field gel electrophoresis type and galU cluster and were members of the same clone. The identity of the clone was confirmed in four isolates by multilocus sequence typing. The sequence type found (ST315) corresponds to the Poland6B-20 clone. In summary, BOX-polymerase chain reaction, pulsed-field gel electrophoresis, and galU polymorphism were useful tools to detect the presence of a clone whose identity was confirmed by multilocus sequence typing. The isolates belonging to Poland6B-20 found in this work are described for the first time in Latin America.Fil: Bonofiglio, Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Regueira, Mabel. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Pace, Julio. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Corso, Alejandra. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: García, Ernesto. Centro de Investigación Biomédica en Red; EspañaFil: Mollerach, Marta Eugenia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaMary Ann Liebert2011-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67317Bonofiglio, Laura; Regueira, Mabel; Pace, Julio; Corso, Alejandra; García, Ernesto; et al.; Dissemination of an erythromycin-resistant penicillin-nonsusceptible streptococcus pneumoniae Poland6B-20 clone in Argentina; Mary Ann Liebert; Microbial Drug Resistance: Mechanisms Epidemiology and Disease; 17; 1; 3-2011; 75-811076-6294CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/10.1089/mdr.2010.0027info:eu-repo/semantics/altIdentifier/doi/10.1089/mdr.2010.0027info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:29Zoai:ri.conicet.gov.ar:11336/67317instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:29.75CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dissemination of an erythromycin-resistant penicillin-nonsusceptible streptococcus pneumoniae Poland6B-20 clone in Argentina
title Dissemination of an erythromycin-resistant penicillin-nonsusceptible streptococcus pneumoniae Poland6B-20 clone in Argentina
spellingShingle Dissemination of an erythromycin-resistant penicillin-nonsusceptible streptococcus pneumoniae Poland6B-20 clone in Argentina
Bonofiglio, Laura
S. Pneumoniae
Serotype 6b
Poland6b-20
Galu
Pfge
title_short Dissemination of an erythromycin-resistant penicillin-nonsusceptible streptococcus pneumoniae Poland6B-20 clone in Argentina
title_full Dissemination of an erythromycin-resistant penicillin-nonsusceptible streptococcus pneumoniae Poland6B-20 clone in Argentina
title_fullStr Dissemination of an erythromycin-resistant penicillin-nonsusceptible streptococcus pneumoniae Poland6B-20 clone in Argentina
title_full_unstemmed Dissemination of an erythromycin-resistant penicillin-nonsusceptible streptococcus pneumoniae Poland6B-20 clone in Argentina
title_sort Dissemination of an erythromycin-resistant penicillin-nonsusceptible streptococcus pneumoniae Poland6B-20 clone in Argentina
dc.creator.none.fl_str_mv Bonofiglio, Laura
Regueira, Mabel
Pace, Julio
Corso, Alejandra
García, Ernesto
Mollerach, Marta Eugenia
author Bonofiglio, Laura
author_facet Bonofiglio, Laura
Regueira, Mabel
Pace, Julio
Corso, Alejandra
García, Ernesto
Mollerach, Marta Eugenia
author_role author
author2 Regueira, Mabel
Pace, Julio
Corso, Alejandra
García, Ernesto
Mollerach, Marta Eugenia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv S. Pneumoniae
Serotype 6b
Poland6b-20
Galu
Pfge
topic S. Pneumoniae
Serotype 6b
Poland6b-20
Galu
Pfge
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Prevalence of serotype 6B penicillin (PEN)-nonsusceptible Streptococcus pneumoniae significantly increased from 15.8% (1993-1997) to 67.3% (1998-2002) (p < 0.001) in Argentina. Serogroup 6 ranks fourth among different capsular types within invasive isolates from Argentinean patients <6 years of age. To evaluate whether the increase in PEN resistance in serotype 6B pneumococci was due to the dissemination of one or more clones, the genetic diversity of 93 S. pneumoniae serotype 6B isolates was analyzed. Five BOX-polymerase chain reaction types were obtained (65.5% isolates) and a group of 15 isolates, representing 41.6% of those having a decreased susceptibility to PEN, were further characterized. The antibiotype of these isolates showed their multiresistance, with 100% of the isolates being resistant to erythromycin, 80% to tetracycline, and 73.3% to trimethoprim-sulfamethoxazole. Of the 15 isolates, 13 belonged to the same pulsed-field gel electrophoresis type and galU cluster and were members of the same clone. The identity of the clone was confirmed in four isolates by multilocus sequence typing. The sequence type found (ST315) corresponds to the Poland6B-20 clone. In summary, BOX-polymerase chain reaction, pulsed-field gel electrophoresis, and galU polymorphism were useful tools to detect the presence of a clone whose identity was confirmed by multilocus sequence typing. The isolates belonging to Poland6B-20 found in this work are described for the first time in Latin America.
Fil: Bonofiglio, Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Regueira, Mabel. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Pace, Julio. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Corso, Alejandra. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: García, Ernesto. Centro de Investigación Biomédica en Red; España
Fil: Mollerach, Marta Eugenia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description Prevalence of serotype 6B penicillin (PEN)-nonsusceptible Streptococcus pneumoniae significantly increased from 15.8% (1993-1997) to 67.3% (1998-2002) (p < 0.001) in Argentina. Serogroup 6 ranks fourth among different capsular types within invasive isolates from Argentinean patients <6 years of age. To evaluate whether the increase in PEN resistance in serotype 6B pneumococci was due to the dissemination of one or more clones, the genetic diversity of 93 S. pneumoniae serotype 6B isolates was analyzed. Five BOX-polymerase chain reaction types were obtained (65.5% isolates) and a group of 15 isolates, representing 41.6% of those having a decreased susceptibility to PEN, were further characterized. The antibiotype of these isolates showed their multiresistance, with 100% of the isolates being resistant to erythromycin, 80% to tetracycline, and 73.3% to trimethoprim-sulfamethoxazole. Of the 15 isolates, 13 belonged to the same pulsed-field gel electrophoresis type and galU cluster and were members of the same clone. The identity of the clone was confirmed in four isolates by multilocus sequence typing. The sequence type found (ST315) corresponds to the Poland6B-20 clone. In summary, BOX-polymerase chain reaction, pulsed-field gel electrophoresis, and galU polymorphism were useful tools to detect the presence of a clone whose identity was confirmed by multilocus sequence typing. The isolates belonging to Poland6B-20 found in this work are described for the first time in Latin America.
publishDate 2011
dc.date.none.fl_str_mv 2011-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67317
Bonofiglio, Laura; Regueira, Mabel; Pace, Julio; Corso, Alejandra; García, Ernesto; et al.; Dissemination of an erythromycin-resistant penicillin-nonsusceptible streptococcus pneumoniae Poland6B-20 clone in Argentina; Mary Ann Liebert; Microbial Drug Resistance: Mechanisms Epidemiology and Disease; 17; 1; 3-2011; 75-81
1076-6294
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67317
identifier_str_mv Bonofiglio, Laura; Regueira, Mabel; Pace, Julio; Corso, Alejandra; García, Ernesto; et al.; Dissemination of an erythromycin-resistant penicillin-nonsusceptible streptococcus pneumoniae Poland6B-20 clone in Argentina; Mary Ann Liebert; Microbial Drug Resistance: Mechanisms Epidemiology and Disease; 17; 1; 3-2011; 75-81
1076-6294
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/10.1089/mdr.2010.0027
info:eu-repo/semantics/altIdentifier/doi/10.1089/mdr.2010.0027
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert
publisher.none.fl_str_mv Mary Ann Liebert
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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