The galU gene expression in Streptococcus pneumoniae

Autores
Bonofiglio, Laura; García, Ernesto; Mollerach, Marta Eugenia
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The polysaccharide capsule of Streptococcus pneumoniae is the main virulence factor making the bacterium resistant to phagocytosis. The galU gene of Streptococcus pneumoniae encodes a UDP-glucose pyrophosphorylase absolutely required for capsule biosynthesis. In silico analyses indicated that galU gene is co-transcribed with the gpdA gene and four putative promoter regions located upstream gpdA were predicted. One of them behaved as a functional promoter in a promoter reporter system. It is conceivable that the sequence responsible for initiating transcription of gpdA-galU operon is an extended -10 site TATGATA(T/G)AAT. Semiquantitative real-time reverse transcription PCR experiments indicated that galU was expressed mainly in the exponential phase of growth.
Fil: Bonofiglio, Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: García, Ernesto. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas; España
Fil: Mollerach, Marta Eugenia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
Promotor
Galu
-10 Extended
S. Pneumoniae
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12866
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The galU gene expression in Streptococcus pneumoniaeBonofiglio, LauraGarcía, ErnestoMollerach, Marta EugeniaPromotorGalu-10 ExtendedS. Pneumoniaehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The polysaccharide capsule of Streptococcus pneumoniae is the main virulence factor making the bacterium resistant to phagocytosis. The galU gene of Streptococcus pneumoniae encodes a UDP-glucose pyrophosphorylase absolutely required for capsule biosynthesis. In silico analyses indicated that galU gene is co-transcribed with the gpdA gene and four putative promoter regions located upstream gpdA were predicted. One of them behaved as a functional promoter in a promoter reporter system. It is conceivable that the sequence responsible for initiating transcription of gpdA-galU operon is an extended -10 site TATGATA(T/G)AAT. Semiquantitative real-time reverse transcription PCR experiments indicated that galU was expressed mainly in the exponential phase of growth.Fil: Bonofiglio, Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: García, Ernesto. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas; EspañaFil: Mollerach, Marta Eugenia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaWiley2012-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12866Bonofiglio, Laura; García, Ernesto; Mollerach, Marta Eugenia; The galU gene expression in Streptococcus pneumoniae; Wiley; Fems Microbiology Letters; 332; 1; 6-2012; 47-530378-1097enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2012.02572.x/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2012.02572.xinfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article-lookup/doi/10.1111/j.1574-6968.2012.02572.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:54:00Zoai:ri.conicet.gov.ar:11336/12866instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:54:00.424CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The galU gene expression in Streptococcus pneumoniae
title The galU gene expression in Streptococcus pneumoniae
spellingShingle The galU gene expression in Streptococcus pneumoniae
Bonofiglio, Laura
Promotor
Galu
-10 Extended
S. Pneumoniae
title_short The galU gene expression in Streptococcus pneumoniae
title_full The galU gene expression in Streptococcus pneumoniae
title_fullStr The galU gene expression in Streptococcus pneumoniae
title_full_unstemmed The galU gene expression in Streptococcus pneumoniae
title_sort The galU gene expression in Streptococcus pneumoniae
dc.creator.none.fl_str_mv Bonofiglio, Laura
García, Ernesto
Mollerach, Marta Eugenia
author Bonofiglio, Laura
author_facet Bonofiglio, Laura
García, Ernesto
Mollerach, Marta Eugenia
author_role author
author2 García, Ernesto
Mollerach, Marta Eugenia
author2_role author
author
dc.subject.none.fl_str_mv Promotor
Galu
-10 Extended
S. Pneumoniae
topic Promotor
Galu
-10 Extended
S. Pneumoniae
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The polysaccharide capsule of Streptococcus pneumoniae is the main virulence factor making the bacterium resistant to phagocytosis. The galU gene of Streptococcus pneumoniae encodes a UDP-glucose pyrophosphorylase absolutely required for capsule biosynthesis. In silico analyses indicated that galU gene is co-transcribed with the gpdA gene and four putative promoter regions located upstream gpdA were predicted. One of them behaved as a functional promoter in a promoter reporter system. It is conceivable that the sequence responsible for initiating transcription of gpdA-galU operon is an extended -10 site TATGATA(T/G)AAT. Semiquantitative real-time reverse transcription PCR experiments indicated that galU was expressed mainly in the exponential phase of growth.
Fil: Bonofiglio, Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: García, Ernesto. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas; España
Fil: Mollerach, Marta Eugenia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description The polysaccharide capsule of Streptococcus pneumoniae is the main virulence factor making the bacterium resistant to phagocytosis. The galU gene of Streptococcus pneumoniae encodes a UDP-glucose pyrophosphorylase absolutely required for capsule biosynthesis. In silico analyses indicated that galU gene is co-transcribed with the gpdA gene and four putative promoter regions located upstream gpdA were predicted. One of them behaved as a functional promoter in a promoter reporter system. It is conceivable that the sequence responsible for initiating transcription of gpdA-galU operon is an extended -10 site TATGATA(T/G)AAT. Semiquantitative real-time reverse transcription PCR experiments indicated that galU was expressed mainly in the exponential phase of growth.
publishDate 2012
dc.date.none.fl_str_mv 2012-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12866
Bonofiglio, Laura; García, Ernesto; Mollerach, Marta Eugenia; The galU gene expression in Streptococcus pneumoniae; Wiley; Fems Microbiology Letters; 332; 1; 6-2012; 47-53
0378-1097
url http://hdl.handle.net/11336/12866
identifier_str_mv Bonofiglio, Laura; García, Ernesto; Mollerach, Marta Eugenia; The galU gene expression in Streptococcus pneumoniae; Wiley; Fems Microbiology Letters; 332; 1; 6-2012; 47-53
0378-1097
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2012.02572.x/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2012.02572.x
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article-lookup/doi/10.1111/j.1574-6968.2012.02572.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.223955

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