Alpha monofluoro substitution at C5 in homotyphasterol enhances shoot production and multiplication rate of in vitro-grown marubakaido apple rootstock shoots

Autores
Pereira Netto, Adaucto B.; Ramirez, Javier Alberto; Galagovsky, Lydia Raquel
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Key message: 5 fluoro-typhasterol is more effective than the most potent natural brassinosteroid, brassinolide, towards stimulation of shoot production in the marubakaido apple rootstock. Abstract: Brassinosteroids (BRs) comprise a class of low-abundance plant steroids required for normal plant growth. Here we demonstrate that treatment of in vitro-grown shoots of the marubakaido apple rootstock with 2.5 µg per shoot of 5α-monofluoro homotyphasterol (5F-HTY), a synthetic derivative of the natural BR homotyphasterol (HTY), resulted in significant enhancement in the formation of primary lateral shoots. This treatment also resulted in increased length of primary lateral shoots and main shoots. This growth-stimulatory effect led to a significant increase in the multiplication rate for the rootstock. In contrast to what was found for 5F-HTY, neither HTY nor the synthetic derivative 3α-monofluoro homotyphasterol (3F-HTY) were able to significantly stimulate shoot formation. HTY and 3F-HTY were not able to stimulate primary lateral shoot elongation as well. However, both HTY and 3F-HTY were able to significantly stimulate main shoot elongation. The 5F-HTY-driven enhancement of the multiplication rate described here demonstrates the potential of this compound to improve micropropagation techniques, not only for the marubakaido apple rootstock.
Fil: Pereira Netto, Adaucto B.. Universidade Tecnologica Federal Do Parana; Brasil
Fil: Ramirez, Javier Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Galagovsky, Lydia Raquel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Materia
BRASSINOLIDE
BRASSINOSTEROID
MALUS PRUNIFOLIA
MICROPROPAGATION
STIGMASTEROL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/123304
Acceder
id CONICETDig_ec0a08c73414fb7415cf14cbdb0ff2c0
oai_identifier_str oai:ri.conicet.gov.ar:11336/123304
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Alpha monofluoro substitution at C5 in homotyphasterol enhances shoot production and multiplication rate of in vitro-grown marubakaido apple rootstock shootsPereira Netto, Adaucto B.Ramirez, Javier AlbertoGalagovsky, Lydia RaquelBRASSINOLIDEBRASSINOSTEROIDMALUS PRUNIFOLIAMICROPROPAGATIONSTIGMASTEROLhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Key message: 5 fluoro-typhasterol is more effective than the most potent natural brassinosteroid, brassinolide, towards stimulation of shoot production in the marubakaido apple rootstock. Abstract: Brassinosteroids (BRs) comprise a class of low-abundance plant steroids required for normal plant growth. Here we demonstrate that treatment of in vitro-grown shoots of the marubakaido apple rootstock with 2.5 µg per shoot of 5α-monofluoro homotyphasterol (5F-HTY), a synthetic derivative of the natural BR homotyphasterol (HTY), resulted in significant enhancement in the formation of primary lateral shoots. This treatment also resulted in increased length of primary lateral shoots and main shoots. This growth-stimulatory effect led to a significant increase in the multiplication rate for the rootstock. In contrast to what was found for 5F-HTY, neither HTY nor the synthetic derivative 3α-monofluoro homotyphasterol (3F-HTY) were able to significantly stimulate shoot formation. HTY and 3F-HTY were not able to stimulate primary lateral shoot elongation as well. However, both HTY and 3F-HTY were able to significantly stimulate main shoot elongation. The 5F-HTY-driven enhancement of the multiplication rate described here demonstrates the potential of this compound to improve micropropagation techniques, not only for the marubakaido apple rootstock.Fil: Pereira Netto, Adaucto B.. Universidade Tecnologica Federal Do Parana; BrasilFil: Ramirez, Javier Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; ArgentinaFil: Galagovsky, Lydia Raquel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; ArgentinaSpringer2019-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/123304Pereira Netto, Adaucto B.; Ramirez, Javier Alberto; Galagovsky, Lydia Raquel; Alpha monofluoro substitution at C5 in homotyphasterol enhances shoot production and multiplication rate of in vitro-grown marubakaido apple rootstock shoots; Springer; Trees; 33; 3; 4-2019; 963-9720931-1890CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s00468-019-01832-7info:eu-repo/semantics/altIdentifier/doi/10.1007/s00468-019-01832-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:31Zoai:ri.conicet.gov.ar:11336/123304instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:31.874CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Alpha monofluoro substitution at C5 in homotyphasterol enhances shoot production and multiplication rate of in vitro-grown marubakaido apple rootstock shoots
title Alpha monofluoro substitution at C5 in homotyphasterol enhances shoot production and multiplication rate of in vitro-grown marubakaido apple rootstock shoots
spellingShingle Alpha monofluoro substitution at C5 in homotyphasterol enhances shoot production and multiplication rate of in vitro-grown marubakaido apple rootstock shoots
Pereira Netto, Adaucto B.
BRASSINOLIDE
BRASSINOSTEROID
MALUS PRUNIFOLIA
MICROPROPAGATION
STIGMASTEROL
title_short Alpha monofluoro substitution at C5 in homotyphasterol enhances shoot production and multiplication rate of in vitro-grown marubakaido apple rootstock shoots
title_full Alpha monofluoro substitution at C5 in homotyphasterol enhances shoot production and multiplication rate of in vitro-grown marubakaido apple rootstock shoots
title_fullStr Alpha monofluoro substitution at C5 in homotyphasterol enhances shoot production and multiplication rate of in vitro-grown marubakaido apple rootstock shoots
title_full_unstemmed Alpha monofluoro substitution at C5 in homotyphasterol enhances shoot production and multiplication rate of in vitro-grown marubakaido apple rootstock shoots
title_sort Alpha monofluoro substitution at C5 in homotyphasterol enhances shoot production and multiplication rate of in vitro-grown marubakaido apple rootstock shoots
dc.creator.none.fl_str_mv Pereira Netto, Adaucto B.
Ramirez, Javier Alberto
Galagovsky, Lydia Raquel
author Pereira Netto, Adaucto B.
author_facet Pereira Netto, Adaucto B.
Ramirez, Javier Alberto
Galagovsky, Lydia Raquel
author_role author
author2 Ramirez, Javier Alberto
Galagovsky, Lydia Raquel
author2_role author
author
dc.subject.none.fl_str_mv BRASSINOLIDE
BRASSINOSTEROID
MALUS PRUNIFOLIA
MICROPROPAGATION
STIGMASTEROL
topic BRASSINOLIDE
BRASSINOSTEROID
MALUS PRUNIFOLIA
MICROPROPAGATION
STIGMASTEROL
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Key message: 5 fluoro-typhasterol is more effective than the most potent natural brassinosteroid, brassinolide, towards stimulation of shoot production in the marubakaido apple rootstock. Abstract: Brassinosteroids (BRs) comprise a class of low-abundance plant steroids required for normal plant growth. Here we demonstrate that treatment of in vitro-grown shoots of the marubakaido apple rootstock with 2.5 µg per shoot of 5α-monofluoro homotyphasterol (5F-HTY), a synthetic derivative of the natural BR homotyphasterol (HTY), resulted in significant enhancement in the formation of primary lateral shoots. This treatment also resulted in increased length of primary lateral shoots and main shoots. This growth-stimulatory effect led to a significant increase in the multiplication rate for the rootstock. In contrast to what was found for 5F-HTY, neither HTY nor the synthetic derivative 3α-monofluoro homotyphasterol (3F-HTY) were able to significantly stimulate shoot formation. HTY and 3F-HTY were not able to stimulate primary lateral shoot elongation as well. However, both HTY and 3F-HTY were able to significantly stimulate main shoot elongation. The 5F-HTY-driven enhancement of the multiplication rate described here demonstrates the potential of this compound to improve micropropagation techniques, not only for the marubakaido apple rootstock.
Fil: Pereira Netto, Adaucto B.. Universidade Tecnologica Federal Do Parana; Brasil
Fil: Ramirez, Javier Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Galagovsky, Lydia Raquel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
description Key message: 5 fluoro-typhasterol is more effective than the most potent natural brassinosteroid, brassinolide, towards stimulation of shoot production in the marubakaido apple rootstock. Abstract: Brassinosteroids (BRs) comprise a class of low-abundance plant steroids required for normal plant growth. Here we demonstrate that treatment of in vitro-grown shoots of the marubakaido apple rootstock with 2.5 µg per shoot of 5α-monofluoro homotyphasterol (5F-HTY), a synthetic derivative of the natural BR homotyphasterol (HTY), resulted in significant enhancement in the formation of primary lateral shoots. This treatment also resulted in increased length of primary lateral shoots and main shoots. This growth-stimulatory effect led to a significant increase in the multiplication rate for the rootstock. In contrast to what was found for 5F-HTY, neither HTY nor the synthetic derivative 3α-monofluoro homotyphasterol (3F-HTY) were able to significantly stimulate shoot formation. HTY and 3F-HTY were not able to stimulate primary lateral shoot elongation as well. However, both HTY and 3F-HTY were able to significantly stimulate main shoot elongation. The 5F-HTY-driven enhancement of the multiplication rate described here demonstrates the potential of this compound to improve micropropagation techniques, not only for the marubakaido apple rootstock.
publishDate 2019
dc.date.none.fl_str_mv 2019-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/123304
Pereira Netto, Adaucto B.; Ramirez, Javier Alberto; Galagovsky, Lydia Raquel; Alpha monofluoro substitution at C5 in homotyphasterol enhances shoot production and multiplication rate of in vitro-grown marubakaido apple rootstock shoots; Springer; Trees; 33; 3; 4-2019; 963-972
0931-1890
CONICET Digital
CONICET
url http://hdl.handle.net/11336/123304
identifier_str_mv Pereira Netto, Adaucto B.; Ramirez, Javier Alberto; Galagovsky, Lydia Raquel; Alpha monofluoro substitution at C5 in homotyphasterol enhances shoot production and multiplication rate of in vitro-grown marubakaido apple rootstock shoots; Springer; Trees; 33; 3; 4-2019; 963-972
0931-1890
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s00468-019-01832-7
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00468-019-01832-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613673968992256
score 13.070432

Publicaciones similares