Frequencies of Salmonella enterica in Growing Pigs in Paraguay

Autores
Copes, Julio Alberto; Leotta, Gerardo Anibal; Cardozo, L.; Gimenez, G.; Nuñez, L.; Zarate, N.; Lopez, D.; Weiler, N.; Alvarez, M.; Suzuki, K.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of this study was to (1) estimate frequencies of Salmonella enterica in growing pigs and (2) investigate farmers? perception and practices towards pig farming in Central Department, Paraguay. Twelve out of 19 districts in the department were selected and 33 farms with growers in the selected districts were recruited. Questionnaire interviews for each study farm, in combination with faecal sample collections (n = 30 per farm), which were microbiologically examined and visual inspection of pig production facilities were performed between March and November 2009. A questionnaire was designed to obtain basic information of a farm such as the number of pigs owned and some selected farming management practices. Faecal samples were used for Salmonella isolation, using buffered peptone water to have salmonellae pre-enriched, followed by xylose lysine tergitol 4 agar and brilliant green sulfapyridine agar. Suspect colonies were biochemically tested by triple sugar iron agar in combination with lysine iron agar to confirm the identity. The true frequency probability and associated 95% Bayesian credible intervals (95% BCI) were computed via the Gibbs sampler, a Markov chain Monte Carlo technique. Overall, 18% (95% BCI: 8-31%) of the tested 1000 faecal samples were classified as positive for Salmonella enterica. All the study farms had at least one positive sample for Salmonella enterica (frequency range: 3-60%). Apparent prevalence at farm-level was therefore 100% (one-sided 97.5% confidence limit: 89%). Twenty-eight different serovars for Salmonella enterica were found. Based on increase the number of study districts, farms as well as animals in combination with improvement of sampling methods, possible spatial differences and risk factors/indicators should be clarified by further investigations.
Fil: Copes, Julio Alberto. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Epizootiología y Salud Publica. Cátedra de Tecnología y Sanidad de Los Alimentos; Argentina
Fil: Leotta, Gerardo Anibal. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Epizootiología y Salud Publica. Cátedra de Tecnología y Sanidad de Los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cardozo, L.. Universidad Nacional de Asunción; Paraguay
Fil: Gimenez, G.. Universidad Nacional de Asunción; Paraguay
Fil: Nuñez, L.. Universidad Nacional de Asunción; Paraguay
Fil: Zarate, N.. Universidad Nacional de Asunción; Paraguay
Fil: Lopez, D.. Ministerio de Salud Pública y Bienestar Social; Paraguay
Fil: Weiler, N.. Ministerio de Salud Pública y Bienestar Social; Paraguay
Fil: Alvarez, M.. Ministerio de Salud Pública y Bienestar Social; Paraguay
Fil: Suzuki, K.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina
Materia
SALMONELLA IN PIGS
GROWING PIGS
SOUTH AMERICA
PARAGUAY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/156530
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/156530
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Frequencies of Salmonella enterica in Growing Pigs in ParaguayCopes, Julio AlbertoLeotta, Gerardo AnibalCardozo, L.Gimenez, G.Nuñez, L.Zarate, N.Lopez, D.Weiler, N.Alvarez, M.Suzuki, K.SALMONELLA IN PIGSGROWING PIGSSOUTH AMERICAPARAGUAYhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The objective of this study was to (1) estimate frequencies of Salmonella enterica in growing pigs and (2) investigate farmers? perception and practices towards pig farming in Central Department, Paraguay. Twelve out of 19 districts in the department were selected and 33 farms with growers in the selected districts were recruited. Questionnaire interviews for each study farm, in combination with faecal sample collections (n = 30 per farm), which were microbiologically examined and visual inspection of pig production facilities were performed between March and November 2009. A questionnaire was designed to obtain basic information of a farm such as the number of pigs owned and some selected farming management practices. Faecal samples were used for Salmonella isolation, using buffered peptone water to have salmonellae pre-enriched, followed by xylose lysine tergitol 4 agar and brilliant green sulfapyridine agar. Suspect colonies were biochemically tested by triple sugar iron agar in combination with lysine iron agar to confirm the identity. The true frequency probability and associated 95% Bayesian credible intervals (95% BCI) were computed via the Gibbs sampler, a Markov chain Monte Carlo technique. Overall, 18% (95% BCI: 8-31%) of the tested 1000 faecal samples were classified as positive for Salmonella enterica. All the study farms had at least one positive sample for Salmonella enterica (frequency range: 3-60%). Apparent prevalence at farm-level was therefore 100% (one-sided 97.5% confidence limit: 89%). Twenty-eight different serovars for Salmonella enterica were found. Based on increase the number of study districts, farms as well as animals in combination with improvement of sampling methods, possible spatial differences and risk factors/indicators should be clarified by further investigations.Fil: Copes, Julio Alberto. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Epizootiología y Salud Publica. Cátedra de Tecnología y Sanidad de Los Alimentos; ArgentinaFil: Leotta, Gerardo Anibal. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Epizootiología y Salud Publica. Cátedra de Tecnología y Sanidad de Los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cardozo, L.. Universidad Nacional de Asunción; ParaguayFil: Gimenez, G.. Universidad Nacional de Asunción; ParaguayFil: Nuñez, L.. Universidad Nacional de Asunción; ParaguayFil: Zarate, N.. Universidad Nacional de Asunción; ParaguayFil: Lopez, D.. Ministerio de Salud Pública y Bienestar Social; ParaguayFil: Weiler, N.. Ministerio de Salud Pública y Bienestar Social; ParaguayFil: Alvarez, M.. Ministerio de Salud Pública y Bienestar Social; ParaguayFil: Suzuki, K.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; ArgentinaScience Alert2011-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/156530Copes, Julio Alberto; Leotta, Gerardo Anibal; Cardozo, L.; Gimenez, G.; Nuñez, L.; et al.; Frequencies of Salmonella enterica in Growing Pigs in Paraguay; Science Alert; Research Journal of Microbiology; 6; 2; 2-2011; 159-1651816-4935CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://scialert.net/abstract/?doi=jm.2011.159.165info:eu-repo/semantics/altIdentifier/doi/10.3923/jm.2011.159.165info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:21Zoai:ri.conicet.gov.ar:11336/156530instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:22.06CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Frequencies of Salmonella enterica in Growing Pigs in Paraguay
title Frequencies of Salmonella enterica in Growing Pigs in Paraguay
spellingShingle Frequencies of Salmonella enterica in Growing Pigs in Paraguay
Copes, Julio Alberto
SALMONELLA IN PIGS
GROWING PIGS
SOUTH AMERICA
PARAGUAY
title_short Frequencies of Salmonella enterica in Growing Pigs in Paraguay
title_full Frequencies of Salmonella enterica in Growing Pigs in Paraguay
title_fullStr Frequencies of Salmonella enterica in Growing Pigs in Paraguay
title_full_unstemmed Frequencies of Salmonella enterica in Growing Pigs in Paraguay
title_sort Frequencies of Salmonella enterica in Growing Pigs in Paraguay
dc.creator.none.fl_str_mv Copes, Julio Alberto
Leotta, Gerardo Anibal
Cardozo, L.
Gimenez, G.
Nuñez, L.
Zarate, N.
Lopez, D.
Weiler, N.
Alvarez, M.
Suzuki, K.
author Copes, Julio Alberto
author_facet Copes, Julio Alberto
Leotta, Gerardo Anibal
Cardozo, L.
Gimenez, G.
Nuñez, L.
Zarate, N.
Lopez, D.
Weiler, N.
Alvarez, M.
Suzuki, K.
author_role author
author2 Leotta, Gerardo Anibal
Cardozo, L.
Gimenez, G.
Nuñez, L.
Zarate, N.
Lopez, D.
Weiler, N.
Alvarez, M.
Suzuki, K.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SALMONELLA IN PIGS
GROWING PIGS
SOUTH AMERICA
PARAGUAY
topic SALMONELLA IN PIGS
GROWING PIGS
SOUTH AMERICA
PARAGUAY
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The objective of this study was to (1) estimate frequencies of Salmonella enterica in growing pigs and (2) investigate farmers? perception and practices towards pig farming in Central Department, Paraguay. Twelve out of 19 districts in the department were selected and 33 farms with growers in the selected districts were recruited. Questionnaire interviews for each study farm, in combination with faecal sample collections (n = 30 per farm), which were microbiologically examined and visual inspection of pig production facilities were performed between March and November 2009. A questionnaire was designed to obtain basic information of a farm such as the number of pigs owned and some selected farming management practices. Faecal samples were used for Salmonella isolation, using buffered peptone water to have salmonellae pre-enriched, followed by xylose lysine tergitol 4 agar and brilliant green sulfapyridine agar. Suspect colonies were biochemically tested by triple sugar iron agar in combination with lysine iron agar to confirm the identity. The true frequency probability and associated 95% Bayesian credible intervals (95% BCI) were computed via the Gibbs sampler, a Markov chain Monte Carlo technique. Overall, 18% (95% BCI: 8-31%) of the tested 1000 faecal samples were classified as positive for Salmonella enterica. All the study farms had at least one positive sample for Salmonella enterica (frequency range: 3-60%). Apparent prevalence at farm-level was therefore 100% (one-sided 97.5% confidence limit: 89%). Twenty-eight different serovars for Salmonella enterica were found. Based on increase the number of study districts, farms as well as animals in combination with improvement of sampling methods, possible spatial differences and risk factors/indicators should be clarified by further investigations.
Fil: Copes, Julio Alberto. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Epizootiología y Salud Publica. Cátedra de Tecnología y Sanidad de Los Alimentos; Argentina
Fil: Leotta, Gerardo Anibal. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Departamento de Epizootiología y Salud Publica. Cátedra de Tecnología y Sanidad de Los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cardozo, L.. Universidad Nacional de Asunción; Paraguay
Fil: Gimenez, G.. Universidad Nacional de Asunción; Paraguay
Fil: Nuñez, L.. Universidad Nacional de Asunción; Paraguay
Fil: Zarate, N.. Universidad Nacional de Asunción; Paraguay
Fil: Lopez, D.. Ministerio de Salud Pública y Bienestar Social; Paraguay
Fil: Weiler, N.. Ministerio de Salud Pública y Bienestar Social; Paraguay
Fil: Alvarez, M.. Ministerio de Salud Pública y Bienestar Social; Paraguay
Fil: Suzuki, K.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina
description The objective of this study was to (1) estimate frequencies of Salmonella enterica in growing pigs and (2) investigate farmers? perception and practices towards pig farming in Central Department, Paraguay. Twelve out of 19 districts in the department were selected and 33 farms with growers in the selected districts were recruited. Questionnaire interviews for each study farm, in combination with faecal sample collections (n = 30 per farm), which were microbiologically examined and visual inspection of pig production facilities were performed between March and November 2009. A questionnaire was designed to obtain basic information of a farm such as the number of pigs owned and some selected farming management practices. Faecal samples were used for Salmonella isolation, using buffered peptone water to have salmonellae pre-enriched, followed by xylose lysine tergitol 4 agar and brilliant green sulfapyridine agar. Suspect colonies were biochemically tested by triple sugar iron agar in combination with lysine iron agar to confirm the identity. The true frequency probability and associated 95% Bayesian credible intervals (95% BCI) were computed via the Gibbs sampler, a Markov chain Monte Carlo technique. Overall, 18% (95% BCI: 8-31%) of the tested 1000 faecal samples were classified as positive for Salmonella enterica. All the study farms had at least one positive sample for Salmonella enterica (frequency range: 3-60%). Apparent prevalence at farm-level was therefore 100% (one-sided 97.5% confidence limit: 89%). Twenty-eight different serovars for Salmonella enterica were found. Based on increase the number of study districts, farms as well as animals in combination with improvement of sampling methods, possible spatial differences and risk factors/indicators should be clarified by further investigations.
publishDate 2011
dc.date.none.fl_str_mv 2011-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/156530
Copes, Julio Alberto; Leotta, Gerardo Anibal; Cardozo, L.; Gimenez, G.; Nuñez, L.; et al.; Frequencies of Salmonella enterica in Growing Pigs in Paraguay; Science Alert; Research Journal of Microbiology; 6; 2; 2-2011; 159-165
1816-4935
CONICET Digital
CONICET
url http://hdl.handle.net/11336/156530
identifier_str_mv Copes, Julio Alberto; Leotta, Gerardo Anibal; Cardozo, L.; Gimenez, G.; Nuñez, L.; et al.; Frequencies of Salmonella enterica in Growing Pigs in Paraguay; Science Alert; Research Journal of Microbiology; 6; 2; 2-2011; 159-165
1816-4935
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://scialert.net/abstract/?doi=jm.2011.159.165
info:eu-repo/semantics/altIdentifier/doi/10.3923/jm.2011.159.165
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Science Alert
publisher.none.fl_str_mv Science Alert
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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