Evaluation of different methods for assessing the reproductive mode of weeping lovegrass plants, Eragrostis curvula (Schrad.) Nees

Autores
Meier, Mauro Sebastián; Zappacosta, Diego Carlos; Selva, Juan Pablo; Pessino, Silvina Claudia; Echenique, Carmen Viviana
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Weeping lovegrass is a forage grass cultivated in semiarid regions of the world that reproduces mainly by apomixis (diplospory), a process that involves the formation of asexual seeds and bypasses the processes of meiosis and fertilisation. The aim of this work was to evaluate and compare different techniques (cytoembryology, callose deposition, flow cytometry and progeny tests) to determine the reproductive mode of weeping lovegrass. Typical sexual and apomictic processes were clearly differentiated using cytoembryology, and different callose deposition patterns were observed in sexual and apomictic genotypes. Previous studies indicated that presence of callose on the cell wall of the megaspore mother cell is associated only with sexual processes. Nevertheless, our results also found callose deposition in apomictic genotypes, although clearly different from the pattern found in sexual processes, allowing discrimination between sexual and apomictic plants. Flow cytometry seed screening using individual seeds did not differentiate between sexual and apomictic plants as the embryo : endosperm DNA content ratio was similar in sexual and apomictic plants. Progeny tests using molecular markers showed uniform patterns in offspring from apomictic plants and variable patterns among the progeny of sexual plants. The results obtained from cytological studies and progeny tests were similar, indicating that both methods provide useful tools for determination of reproductive mode. However, the callose test with aniline blue was faster and easier to use than other techniques.
Fil: Meier, Mauro Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Fil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Fil: Selva, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Materia
Weeping Lovegrass
Apomixis
Callose
Cytology
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16067

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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of different methods for assessing the reproductive mode of weeping lovegrass plants, Eragrostis curvula (Schrad.) NeesMeier, Mauro SebastiánZappacosta, Diego CarlosSelva, Juan PabloPessino, Silvina ClaudiaEchenique, Carmen VivianaWeeping LovegrassApomixisCalloseCytologyhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Weeping lovegrass is a forage grass cultivated in semiarid regions of the world that reproduces mainly by apomixis (diplospory), a process that involves the formation of asexual seeds and bypasses the processes of meiosis and fertilisation. The aim of this work was to evaluate and compare different techniques (cytoembryology, callose deposition, flow cytometry and progeny tests) to determine the reproductive mode of weeping lovegrass. Typical sexual and apomictic processes were clearly differentiated using cytoembryology, and different callose deposition patterns were observed in sexual and apomictic genotypes. Previous studies indicated that presence of callose on the cell wall of the megaspore mother cell is associated only with sexual processes. Nevertheless, our results also found callose deposition in apomictic genotypes, although clearly different from the pattern found in sexual processes, allowing discrimination between sexual and apomictic plants. Flow cytometry seed screening using individual seeds did not differentiate between sexual and apomictic plants as the embryo : endosperm DNA content ratio was similar in sexual and apomictic plants. Progeny tests using molecular markers showed uniform patterns in offspring from apomictic plants and variable patterns among the progeny of sexual plants. The results obtained from cytological studies and progeny tests were similar, indicating that both methods provide useful tools for determination of reproductive mode. However, the callose test with aniline blue was faster and easier to use than other techniques.Fil: Meier, Mauro Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; ArgentinaFil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; ArgentinaFil: Selva, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; ArgentinaFil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; ArgentinaCsiro Publishing2011-05-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16067Meier, Mauro Sebastián; Zappacosta, Diego Carlos; Selva, Juan Pablo; Pessino, Silvina Claudia; Echenique, Carmen Viviana; Evaluation of different methods for assessing the reproductive mode of weeping lovegrass plants, Eragrostis curvula (Schrad.) Nees; Csiro Publishing; Australian Journal Of Botany; 59; 3; 9-5-2011; 253-2610067-19241444-9862enginfo:eu-repo/semantics/altIdentifier/url/http://www.publish.csiro.au/BT/BT10267info:eu-repo/semantics/altIdentifier/doi/10.1071/BT10267info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:19:51Zoai:ri.conicet.gov.ar:11336/16067instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:19:52.192CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of different methods for assessing the reproductive mode of weeping lovegrass plants, Eragrostis curvula (Schrad.) Nees
title Evaluation of different methods for assessing the reproductive mode of weeping lovegrass plants, Eragrostis curvula (Schrad.) Nees
spellingShingle Evaluation of different methods for assessing the reproductive mode of weeping lovegrass plants, Eragrostis curvula (Schrad.) Nees
Meier, Mauro Sebastián
Weeping Lovegrass
Apomixis
Callose
Cytology
title_short Evaluation of different methods for assessing the reproductive mode of weeping lovegrass plants, Eragrostis curvula (Schrad.) Nees
title_full Evaluation of different methods for assessing the reproductive mode of weeping lovegrass plants, Eragrostis curvula (Schrad.) Nees
title_fullStr Evaluation of different methods for assessing the reproductive mode of weeping lovegrass plants, Eragrostis curvula (Schrad.) Nees
title_full_unstemmed Evaluation of different methods for assessing the reproductive mode of weeping lovegrass plants, Eragrostis curvula (Schrad.) Nees
title_sort Evaluation of different methods for assessing the reproductive mode of weeping lovegrass plants, Eragrostis curvula (Schrad.) Nees
dc.creator.none.fl_str_mv Meier, Mauro Sebastián
Zappacosta, Diego Carlos
Selva, Juan Pablo
Pessino, Silvina Claudia
Echenique, Carmen Viviana
author Meier, Mauro Sebastián
author_facet Meier, Mauro Sebastián
Zappacosta, Diego Carlos
Selva, Juan Pablo
Pessino, Silvina Claudia
Echenique, Carmen Viviana
author_role author
author2 Zappacosta, Diego Carlos
Selva, Juan Pablo
Pessino, Silvina Claudia
Echenique, Carmen Viviana
author2_role author
author
author
author
dc.subject.none.fl_str_mv Weeping Lovegrass
Apomixis
Callose
Cytology
topic Weeping Lovegrass
Apomixis
Callose
Cytology
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Weeping lovegrass is a forage grass cultivated in semiarid regions of the world that reproduces mainly by apomixis (diplospory), a process that involves the formation of asexual seeds and bypasses the processes of meiosis and fertilisation. The aim of this work was to evaluate and compare different techniques (cytoembryology, callose deposition, flow cytometry and progeny tests) to determine the reproductive mode of weeping lovegrass. Typical sexual and apomictic processes were clearly differentiated using cytoembryology, and different callose deposition patterns were observed in sexual and apomictic genotypes. Previous studies indicated that presence of callose on the cell wall of the megaspore mother cell is associated only with sexual processes. Nevertheless, our results also found callose deposition in apomictic genotypes, although clearly different from the pattern found in sexual processes, allowing discrimination between sexual and apomictic plants. Flow cytometry seed screening using individual seeds did not differentiate between sexual and apomictic plants as the embryo : endosperm DNA content ratio was similar in sexual and apomictic plants. Progeny tests using molecular markers showed uniform patterns in offspring from apomictic plants and variable patterns among the progeny of sexual plants. The results obtained from cytological studies and progeny tests were similar, indicating that both methods provide useful tools for determination of reproductive mode. However, the callose test with aniline blue was faster and easier to use than other techniques.
Fil: Meier, Mauro Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Fil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Fil: Selva, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
description Weeping lovegrass is a forage grass cultivated in semiarid regions of the world that reproduces mainly by apomixis (diplospory), a process that involves the formation of asexual seeds and bypasses the processes of meiosis and fertilisation. The aim of this work was to evaluate and compare different techniques (cytoembryology, callose deposition, flow cytometry and progeny tests) to determine the reproductive mode of weeping lovegrass. Typical sexual and apomictic processes were clearly differentiated using cytoembryology, and different callose deposition patterns were observed in sexual and apomictic genotypes. Previous studies indicated that presence of callose on the cell wall of the megaspore mother cell is associated only with sexual processes. Nevertheless, our results also found callose deposition in apomictic genotypes, although clearly different from the pattern found in sexual processes, allowing discrimination between sexual and apomictic plants. Flow cytometry seed screening using individual seeds did not differentiate between sexual and apomictic plants as the embryo : endosperm DNA content ratio was similar in sexual and apomictic plants. Progeny tests using molecular markers showed uniform patterns in offspring from apomictic plants and variable patterns among the progeny of sexual plants. The results obtained from cytological studies and progeny tests were similar, indicating that both methods provide useful tools for determination of reproductive mode. However, the callose test with aniline blue was faster and easier to use than other techniques.
publishDate 2011
dc.date.none.fl_str_mv 2011-05-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16067
Meier, Mauro Sebastián; Zappacosta, Diego Carlos; Selva, Juan Pablo; Pessino, Silvina Claudia; Echenique, Carmen Viviana; Evaluation of different methods for assessing the reproductive mode of weeping lovegrass plants, Eragrostis curvula (Schrad.) Nees; Csiro Publishing; Australian Journal Of Botany; 59; 3; 9-5-2011; 253-261
0067-1924
1444-9862
url http://hdl.handle.net/11336/16067
identifier_str_mv Meier, Mauro Sebastián; Zappacosta, Diego Carlos; Selva, Juan Pablo; Pessino, Silvina Claudia; Echenique, Carmen Viviana; Evaluation of different methods for assessing the reproductive mode of weeping lovegrass plants, Eragrostis curvula (Schrad.) Nees; Csiro Publishing; Australian Journal Of Botany; 59; 3; 9-5-2011; 253-261
0067-1924
1444-9862
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.publish.csiro.au/BT/BT10267
info:eu-repo/semantics/altIdentifier/doi/10.1071/BT10267
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Csiro Publishing
publisher.none.fl_str_mv Csiro Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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