Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109

Autores
Torres, Daniela Soledad; Mongiardini, Elias Javier; Donadío, Evelyn Florencia; Donoso, Raúl; Recabarren Gajardo, Gonzalo; Gualpa, José Luis; Spaepen, Stijn; Defez, Roberto; Lopez, Gaston Alberto; Bianco, Carmen; Cassan, Fabricio Dario
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bradyrhizobium japonicum E109 is a bacterium widely used for inoculants production in Argentina. It is known for its ability to produce several phytohormones and degrade indole-3-acetic acid (IAA). The genome sequence of B. japonicum E109 was recently analyzed and it showed the presence of genes related to the synthesis of IAA by indole-3-acetonitrile, indole-3-acetamide and tryptamine pathways. Nevertheless, B. japonicum E109 is not able to produce IAA and instead has the ability to degrade this hormone under saprophytic culture conditions. This work aimed to study the molecular and physiological features of IAA degradation and identify the genes responsible of this activity. In B. japonicum E109 we identified two sequences coding for a putative 3-phenylpropionate dioxygenase (subunits α and β) responsible for the IAA degradation that were homologous to the canonical cluster of iacC and iacD of Pseudomonas putida 1290. These genes form a separate cluster together with three additional genes with unknown functions. The degradation activity was found to be constitutively expressed in B. japonicum E109. As products of IAA degradation, we identified two compounds, 3-indoleacetic acid 2,3-oxide and 2-(2-hydroperoxy-3-hydroxyindolin-3-yl) acetic acid. Our report proposes, for the first time, a model for IAA degradation in Bradyrhizobium.
Fil: Torres, Daniela Soledad. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina
Fil: Mongiardini, Elias Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; Argentina
Fil: Donadío, Evelyn Florencia. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina
Fil: Donoso, Raúl. Universidad Tecnológica Metropolitana; Chile
Fil: Recabarren Gajardo, Gonzalo. Pontificia Universidad Católica de Chile; Chile
Fil: Gualpa, José Luis. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina
Fil: Spaepen, Stijn. Katholikie Universiteit Leuven; Bélgica
Fil: Defez, Roberto. Institute Of Biosciences And Bioresources; Italia
Fil: Lopez, Gaston Alberto. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina
Fil: Bianco, Carmen. Institute Of Biosciences And Bioresources; Italia
Fil: Cassan, Fabricio Dario. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina
Materia
2-(2-HYDROPEROXY-3-HYDROXYINDOLIN-3-YL) ACETIC ACID
3-INDOLEACETIC ACID 2
3-OXIDE
3-PHENYLPROPIONATE DIOXYGENASE
B. JAPONICUM
INDOLE-3-ACETIC ACID
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/180980
Acceder
id CONICETDig_ebc06e6b7d17aff41e3925dc8a8efc5e
oai_identifier_str oai:ri.conicet.gov.ar:11336/180980
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109Torres, Daniela SoledadMongiardini, Elias JavierDonadío, Evelyn FlorenciaDonoso, RaúlRecabarren Gajardo, GonzaloGualpa, José LuisSpaepen, StijnDefez, RobertoLopez, Gaston AlbertoBianco, CarmenCassan, Fabricio Dario2-(2-HYDROPEROXY-3-HYDROXYINDOLIN-3-YL) ACETIC ACID3-INDOLEACETIC ACID 23-OXIDE3-PHENYLPROPIONATE DIOXYGENASEB. JAPONICUMINDOLE-3-ACETIC ACIDhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bradyrhizobium japonicum E109 is a bacterium widely used for inoculants production in Argentina. It is known for its ability to produce several phytohormones and degrade indole-3-acetic acid (IAA). The genome sequence of B. japonicum E109 was recently analyzed and it showed the presence of genes related to the synthesis of IAA by indole-3-acetonitrile, indole-3-acetamide and tryptamine pathways. Nevertheless, B. japonicum E109 is not able to produce IAA and instead has the ability to degrade this hormone under saprophytic culture conditions. This work aimed to study the molecular and physiological features of IAA degradation and identify the genes responsible of this activity. In B. japonicum E109 we identified two sequences coding for a putative 3-phenylpropionate dioxygenase (subunits α and β) responsible for the IAA degradation that were homologous to the canonical cluster of iacC and iacD of Pseudomonas putida 1290. These genes form a separate cluster together with three additional genes with unknown functions. The degradation activity was found to be constitutively expressed in B. japonicum E109. As products of IAA degradation, we identified two compounds, 3-indoleacetic acid 2,3-oxide and 2-(2-hydroperoxy-3-hydroxyindolin-3-yl) acetic acid. Our report proposes, for the first time, a model for IAA degradation in Bradyrhizobium.Fil: Torres, Daniela Soledad. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; ArgentinaFil: Mongiardini, Elias Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; ArgentinaFil: Donadío, Evelyn Florencia. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; ArgentinaFil: Donoso, Raúl. Universidad Tecnológica Metropolitana; ChileFil: Recabarren Gajardo, Gonzalo. Pontificia Universidad Católica de Chile; ChileFil: Gualpa, José Luis. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; ArgentinaFil: Spaepen, Stijn. Katholikie Universiteit Leuven; BélgicaFil: Defez, Roberto. Institute Of Biosciences And Bioresources; ItaliaFil: Lopez, Gaston Alberto. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; ArgentinaFil: Bianco, Carmen. Institute Of Biosciences And Bioresources; ItaliaFil: Cassan, Fabricio Dario. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; ArgentinaElsevier Science2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/180980Torres, Daniela Soledad; Mongiardini, Elias Javier; Donadío, Evelyn Florencia; Donoso, Raúl; Recabarren Gajardo, Gonzalo; et al.; Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109; Elsevier Science; Research In Microbiology; 172; 3; 2-2021; 1-470923-2508CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0923250821000279info:eu-repo/semantics/altIdentifier/doi/10.1016/j.resmic.2021.103814info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:42:49Zoai:ri.conicet.gov.ar:11336/180980instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:42:49.903CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109
title Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109
spellingShingle Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109
Torres, Daniela Soledad
2-(2-HYDROPEROXY-3-HYDROXYINDOLIN-3-YL) ACETIC ACID
3-INDOLEACETIC ACID 2
3-OXIDE
3-PHENYLPROPIONATE DIOXYGENASE
B. JAPONICUM
INDOLE-3-ACETIC ACID
title_short Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109
title_full Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109
title_fullStr Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109
title_full_unstemmed Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109
title_sort Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109
dc.creator.none.fl_str_mv Torres, Daniela Soledad
Mongiardini, Elias Javier
Donadío, Evelyn Florencia
Donoso, Raúl
Recabarren Gajardo, Gonzalo
Gualpa, José Luis
Spaepen, Stijn
Defez, Roberto
Lopez, Gaston Alberto
Bianco, Carmen
Cassan, Fabricio Dario
author Torres, Daniela Soledad
author_facet Torres, Daniela Soledad
Mongiardini, Elias Javier
Donadío, Evelyn Florencia
Donoso, Raúl
Recabarren Gajardo, Gonzalo
Gualpa, José Luis
Spaepen, Stijn
Defez, Roberto
Lopez, Gaston Alberto
Bianco, Carmen
Cassan, Fabricio Dario
author_role author
author2 Mongiardini, Elias Javier
Donadío, Evelyn Florencia
Donoso, Raúl
Recabarren Gajardo, Gonzalo
Gualpa, José Luis
Spaepen, Stijn
Defez, Roberto
Lopez, Gaston Alberto
Bianco, Carmen
Cassan, Fabricio Dario
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv 2-(2-HYDROPEROXY-3-HYDROXYINDOLIN-3-YL) ACETIC ACID
3-INDOLEACETIC ACID 2
3-OXIDE
3-PHENYLPROPIONATE DIOXYGENASE
B. JAPONICUM
INDOLE-3-ACETIC ACID
topic 2-(2-HYDROPEROXY-3-HYDROXYINDOLIN-3-YL) ACETIC ACID
3-INDOLEACETIC ACID 2
3-OXIDE
3-PHENYLPROPIONATE DIOXYGENASE
B. JAPONICUM
INDOLE-3-ACETIC ACID
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bradyrhizobium japonicum E109 is a bacterium widely used for inoculants production in Argentina. It is known for its ability to produce several phytohormones and degrade indole-3-acetic acid (IAA). The genome sequence of B. japonicum E109 was recently analyzed and it showed the presence of genes related to the synthesis of IAA by indole-3-acetonitrile, indole-3-acetamide and tryptamine pathways. Nevertheless, B. japonicum E109 is not able to produce IAA and instead has the ability to degrade this hormone under saprophytic culture conditions. This work aimed to study the molecular and physiological features of IAA degradation and identify the genes responsible of this activity. In B. japonicum E109 we identified two sequences coding for a putative 3-phenylpropionate dioxygenase (subunits α and β) responsible for the IAA degradation that were homologous to the canonical cluster of iacC and iacD of Pseudomonas putida 1290. These genes form a separate cluster together with three additional genes with unknown functions. The degradation activity was found to be constitutively expressed in B. japonicum E109. As products of IAA degradation, we identified two compounds, 3-indoleacetic acid 2,3-oxide and 2-(2-hydroperoxy-3-hydroxyindolin-3-yl) acetic acid. Our report proposes, for the first time, a model for IAA degradation in Bradyrhizobium.
Fil: Torres, Daniela Soledad. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina
Fil: Mongiardini, Elias Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; Argentina
Fil: Donadío, Evelyn Florencia. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina
Fil: Donoso, Raúl. Universidad Tecnológica Metropolitana; Chile
Fil: Recabarren Gajardo, Gonzalo. Pontificia Universidad Católica de Chile; Chile
Fil: Gualpa, José Luis. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina
Fil: Spaepen, Stijn. Katholikie Universiteit Leuven; Bélgica
Fil: Defez, Roberto. Institute Of Biosciences And Bioresources; Italia
Fil: Lopez, Gaston Alberto. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina
Fil: Bianco, Carmen. Institute Of Biosciences And Bioresources; Italia
Fil: Cassan, Fabricio Dario. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina
description Bradyrhizobium japonicum E109 is a bacterium widely used for inoculants production in Argentina. It is known for its ability to produce several phytohormones and degrade indole-3-acetic acid (IAA). The genome sequence of B. japonicum E109 was recently analyzed and it showed the presence of genes related to the synthesis of IAA by indole-3-acetonitrile, indole-3-acetamide and tryptamine pathways. Nevertheless, B. japonicum E109 is not able to produce IAA and instead has the ability to degrade this hormone under saprophytic culture conditions. This work aimed to study the molecular and physiological features of IAA degradation and identify the genes responsible of this activity. In B. japonicum E109 we identified two sequences coding for a putative 3-phenylpropionate dioxygenase (subunits α and β) responsible for the IAA degradation that were homologous to the canonical cluster of iacC and iacD of Pseudomonas putida 1290. These genes form a separate cluster together with three additional genes with unknown functions. The degradation activity was found to be constitutively expressed in B. japonicum E109. As products of IAA degradation, we identified two compounds, 3-indoleacetic acid 2,3-oxide and 2-(2-hydroperoxy-3-hydroxyindolin-3-yl) acetic acid. Our report proposes, for the first time, a model for IAA degradation in Bradyrhizobium.
publishDate 2021
dc.date.none.fl_str_mv 2021-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/180980
Torres, Daniela Soledad; Mongiardini, Elias Javier; Donadío, Evelyn Florencia; Donoso, Raúl; Recabarren Gajardo, Gonzalo; et al.; Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109; Elsevier Science; Research In Microbiology; 172; 3; 2-2021; 1-47
0923-2508
CONICET Digital
CONICET
url http://hdl.handle.net/11336/180980
identifier_str_mv Torres, Daniela Soledad; Mongiardini, Elias Javier; Donadío, Evelyn Florencia; Donoso, Raúl; Recabarren Gajardo, Gonzalo; et al.; Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109; Elsevier Science; Research In Microbiology; 172; 3; 2-2021; 1-47
0923-2508
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0923250821000279
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.resmic.2021.103814
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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