Solid phase extraction/cyclodextrin-modified micellar electrokinetic chromatography for the analysis of melatonin and related indole compounds in plants

Autores
Gomez, Federico Jose Vicente; Gatica Hernández, Ismaél Jairo Gabriel; Cerutti, Estela Soledad; Silva, Maria Fernanda
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The identification of melatonin in plants has inspired new investigations to understand its biological function. In this work, a robust, reliable, low-cost, quick and simple method based on solid phase extraction followed by micellar electrokinetic chromatography for the extraction, preconcentration and simultaneous determination of melatonin, tryptophan, serotonin and indole-3-acetic acid in plant material is proposed. Extraction of indole compounds from plant tissues was enhanced by ultrasound and solid phase extraction, which was carried out with C8 SPE cartridges. The use of a dual pseudostationary phase system, involving a mixture of SDS anionic micelles and β-cyclodextrin, enabled to reach adequate selectivity. A BGE of 10 mM sodium tetraborate (pH 9.2), containing 20 mM β-CD, 20 mM SDS, and 10% (v/v) of acetonitrile, allowed baseline separation in less than 10 min. The proposed methodology provided limits of detection (LODs) down to low ppb levels. Under the optimal conditions, a successful application on Arabidopsis tissue, green, and linden tea leaves confirmed the validity of this method for food analysis and as a tool to contribute to the elucidation of the biological role of melatonin in plants.
Fil: Gomez, Federico Jose Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Gatica Hernández, Ismaél Jairo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina
Materia
Cd-Mekc
Melatonin
Tryptophan
Serotonin
Indole-3-Acetic Acid
Plants
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5757
Acceder
id CONICETDig_c07790204586981d28b40100ea8d4872
oai_identifier_str oai:ri.conicet.gov.ar:11336/5757
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Solid phase extraction/cyclodextrin-modified micellar electrokinetic chromatography for the analysis of melatonin and related indole compounds in plantsGomez, Federico Jose VicenteGatica Hernández, Ismaél Jairo GabrielCerutti, Estela SoledadSilva, Maria FernandaCd-MekcMelatoninTryptophanSerotoninIndole-3-Acetic AcidPlantshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The identification of melatonin in plants has inspired new investigations to understand its biological function. In this work, a robust, reliable, low-cost, quick and simple method based on solid phase extraction followed by micellar electrokinetic chromatography for the extraction, preconcentration and simultaneous determination of melatonin, tryptophan, serotonin and indole-3-acetic acid in plant material is proposed. Extraction of indole compounds from plant tissues was enhanced by ultrasound and solid phase extraction, which was carried out with C8 SPE cartridges. The use of a dual pseudostationary phase system, involving a mixture of SDS anionic micelles and β-cyclodextrin, enabled to reach adequate selectivity. A BGE of 10 mM sodium tetraborate (pH 9.2), containing 20 mM β-CD, 20 mM SDS, and 10% (v/v) of acetonitrile, allowed baseline separation in less than 10 min. The proposed methodology provided limits of detection (LODs) down to low ppb levels. Under the optimal conditions, a successful application on Arabidopsis tissue, green, and linden tea leaves confirmed the validity of this method for food analysis and as a tool to contribute to the elucidation of the biological role of melatonin in plants.Fil: Gomez, Federico Jose Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Gatica Hernández, Ismaél Jairo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Biología Agrícola de Mendoza; ArgentinaElsevier2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5757Gomez, Federico Jose Vicente; Gatica Hernández, Ismaél Jairo Gabriel; Cerutti, Estela Soledad; Silva, Maria Fernanda; Solid phase extraction/cyclodextrin-modified micellar electrokinetic chromatography for the analysis of melatonin and related indole compounds in plants; Elsevier; Microchemical Journal; 123; 5-2015; 22-270026-265Xenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0026265X15000971info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2015.05.013info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:14:54Zoai:ri.conicet.gov.ar:11336/5757instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:14:54.65CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Solid phase extraction/cyclodextrin-modified micellar electrokinetic chromatography for the analysis of melatonin and related indole compounds in plants
title Solid phase extraction/cyclodextrin-modified micellar electrokinetic chromatography for the analysis of melatonin and related indole compounds in plants
spellingShingle Solid phase extraction/cyclodextrin-modified micellar electrokinetic chromatography for the analysis of melatonin and related indole compounds in plants
Gomez, Federico Jose Vicente
Cd-Mekc
Melatonin
Tryptophan
Serotonin
Indole-3-Acetic Acid
Plants
title_short Solid phase extraction/cyclodextrin-modified micellar electrokinetic chromatography for the analysis of melatonin and related indole compounds in plants
title_full Solid phase extraction/cyclodextrin-modified micellar electrokinetic chromatography for the analysis of melatonin and related indole compounds in plants
title_fullStr Solid phase extraction/cyclodextrin-modified micellar electrokinetic chromatography for the analysis of melatonin and related indole compounds in plants
title_full_unstemmed Solid phase extraction/cyclodextrin-modified micellar electrokinetic chromatography for the analysis of melatonin and related indole compounds in plants
title_sort Solid phase extraction/cyclodextrin-modified micellar electrokinetic chromatography for the analysis of melatonin and related indole compounds in plants
dc.creator.none.fl_str_mv Gomez, Federico Jose Vicente
Gatica Hernández, Ismaél Jairo Gabriel
Cerutti, Estela Soledad
Silva, Maria Fernanda
author Gomez, Federico Jose Vicente
author_facet Gomez, Federico Jose Vicente
Gatica Hernández, Ismaél Jairo Gabriel
Cerutti, Estela Soledad
Silva, Maria Fernanda
author_role author
author2 Gatica Hernández, Ismaél Jairo Gabriel
Cerutti, Estela Soledad
Silva, Maria Fernanda
author2_role author
author
author
dc.subject.none.fl_str_mv Cd-Mekc
Melatonin
Tryptophan
Serotonin
Indole-3-Acetic Acid
Plants
topic Cd-Mekc
Melatonin
Tryptophan
Serotonin
Indole-3-Acetic Acid
Plants
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The identification of melatonin in plants has inspired new investigations to understand its biological function. In this work, a robust, reliable, low-cost, quick and simple method based on solid phase extraction followed by micellar electrokinetic chromatography for the extraction, preconcentration and simultaneous determination of melatonin, tryptophan, serotonin and indole-3-acetic acid in plant material is proposed. Extraction of indole compounds from plant tissues was enhanced by ultrasound and solid phase extraction, which was carried out with C8 SPE cartridges. The use of a dual pseudostationary phase system, involving a mixture of SDS anionic micelles and β-cyclodextrin, enabled to reach adequate selectivity. A BGE of 10 mM sodium tetraborate (pH 9.2), containing 20 mM β-CD, 20 mM SDS, and 10% (v/v) of acetonitrile, allowed baseline separation in less than 10 min. The proposed methodology provided limits of detection (LODs) down to low ppb levels. Under the optimal conditions, a successful application on Arabidopsis tissue, green, and linden tea leaves confirmed the validity of this method for food analysis and as a tool to contribute to the elucidation of the biological role of melatonin in plants.
Fil: Gomez, Federico Jose Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Gatica Hernández, Ismaél Jairo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina
description The identification of melatonin in plants has inspired new investigations to understand its biological function. In this work, a robust, reliable, low-cost, quick and simple method based on solid phase extraction followed by micellar electrokinetic chromatography for the extraction, preconcentration and simultaneous determination of melatonin, tryptophan, serotonin and indole-3-acetic acid in plant material is proposed. Extraction of indole compounds from plant tissues was enhanced by ultrasound and solid phase extraction, which was carried out with C8 SPE cartridges. The use of a dual pseudostationary phase system, involving a mixture of SDS anionic micelles and β-cyclodextrin, enabled to reach adequate selectivity. A BGE of 10 mM sodium tetraborate (pH 9.2), containing 20 mM β-CD, 20 mM SDS, and 10% (v/v) of acetonitrile, allowed baseline separation in less than 10 min. The proposed methodology provided limits of detection (LODs) down to low ppb levels. Under the optimal conditions, a successful application on Arabidopsis tissue, green, and linden tea leaves confirmed the validity of this method for food analysis and as a tool to contribute to the elucidation of the biological role of melatonin in plants.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5757
Gomez, Federico Jose Vicente; Gatica Hernández, Ismaél Jairo Gabriel; Cerutti, Estela Soledad; Silva, Maria Fernanda; Solid phase extraction/cyclodextrin-modified micellar electrokinetic chromatography for the analysis of melatonin and related indole compounds in plants; Elsevier; Microchemical Journal; 123; 5-2015; 22-27
0026-265X
url http://hdl.handle.net/11336/5757
identifier_str_mv Gomez, Federico Jose Vicente; Gatica Hernández, Ismaél Jairo Gabriel; Cerutti, Estela Soledad; Silva, Maria Fernanda; Solid phase extraction/cyclodextrin-modified micellar electrokinetic chromatography for the analysis of melatonin and related indole compounds in plants; Elsevier; Microchemical Journal; 123; 5-2015; 22-27
0026-265X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0026265X15000971
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2015.05.013
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781574485377024
score 12.982451

Publicaciones similares