Altering microtubule polymerization in Sertoli cells strongly affects their lipid metabolism

Autores
Picardi, María Victoria; Furland, Natalia Edith; Aveldaño, Marta Isabel; Valles, Ana Sofia
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The aim of this work was to study the biological consequences of altering microtubule polymerization in Sertoli cells, with a special focus on their lipid metabolism. For this purpose, the effect of longterm exposures to nocodazole (NCZ) (0.01 µM) was studied on TM4 cells in culture. In addition to the expected effect of NCZ on the depolymerization of tubulin microtubules, a remarkable reorganization was observed in the intermediate flament network (vimentin), which showed a more peripheral distribution than in untreated cells. As compared to cells cultured in control conditions, those exposed to NCZ displayed an increased mitochondrial membrane depolarization (p<0.001). Their plasma membrane integrity was concomitantly altered, as shown by a considerable increase in the leakage of lactate dehydrogenase (p<0.01) released into the culture medium. In addition, the cell proliferation was signifcantly reduced (p<0.003) in NCZ-exposed cells. That the drug induced a major alteration on lipid biosynthesis was clearly apparent from a signifcant increase in triacylglycerol (TAG) levels (p<0.0002) and a massive accumulation of lipid droplets in the cytoplasm of cells. This observation was sustained by following the incorporation and distribution of labeled arachidonic acid ([3H]AA). After 1 hour of incubation, the most highly [3H]-labeled lipid class were TAG in control cells, but choline glycerophospholipids in drug-treated cells. Interestingly 72 hours later, the percentages of label in TAG and cholesterol esters were about twice higher in NCZ-treated than in control cells. We conclude that the cytoskeletal alterations that Sertoli cells undergo after being exposed to NCZ, through their impact on mitochondrial functions and cytoplasmic architecture, are closely involved in the deranged lipid metabolism induced by the drug.
Fil: Picardi, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LXII Reunión Científica de la Sociedad Argentina de Investigación Clínica; LIII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica ; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de Protozoología
Buenos Aires
Argentina
Sociedad Argentina de Investigacion Clinica
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Sociedad Argentina de Inmunología
Sociedad Argentina de Andrologia
Sociedad Argentina de Biofísica
Sociedad Argentina de Biologia
Sociedad Argentina de Farmacología Experimental
Sociedad Argentina de Fisiologia
Sociedad Argentina de Hematologia
Sociedad Argentina de Protozoologia
Materia
Nocodazole
mitochondria
lipids
cytoskeleton
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/157343
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Altering microtubule polymerization in Sertoli cells strongly affects their lipid metabolismPicardi, María VictoriaFurland, Natalia EdithAveldaño, Marta IsabelValles, Ana SofiaNocodazolemitochondrialipidscytoskeletonhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this work was to study the biological consequences of altering microtubule polymerization in Sertoli cells, with a special focus on their lipid metabolism. For this purpose, the effect of longterm exposures to nocodazole (NCZ) (0.01 µM) was studied on TM4 cells in culture. In addition to the expected effect of NCZ on the depolymerization of tubulin microtubules, a remarkable reorganization was observed in the intermediate flament network (vimentin), which showed a more peripheral distribution than in untreated cells. As compared to cells cultured in control conditions, those exposed to NCZ displayed an increased mitochondrial membrane depolarization (p<0.001). Their plasma membrane integrity was concomitantly altered, as shown by a considerable increase in the leakage of lactate dehydrogenase (p<0.01) released into the culture medium. In addition, the cell proliferation was signifcantly reduced (p<0.003) in NCZ-exposed cells. That the drug induced a major alteration on lipid biosynthesis was clearly apparent from a signifcant increase in triacylglycerol (TAG) levels (p<0.0002) and a massive accumulation of lipid droplets in the cytoplasm of cells. This observation was sustained by following the incorporation and distribution of labeled arachidonic acid ([3H]AA). After 1 hour of incubation, the most highly [3H]-labeled lipid class were TAG in control cells, but choline glycerophospholipids in drug-treated cells. Interestingly 72 hours later, the percentages of label in TAG and cholesterol esters were about twice higher in NCZ-treated than in control cells. We conclude that the cytoskeletal alterations that Sertoli cells undergo after being exposed to NCZ, through their impact on mitochondrial functions and cytoplasmic architecture, are closely involved in the deranged lipid metabolism induced by the drug.Fil: Picardi, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaLXII Reunión Científica de la Sociedad Argentina de Investigación Clínica; LIII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica ; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de ProtozoologíaBuenos AiresArgentinaSociedad Argentina de Investigacion ClinicaSociedad Argentina de Investigación Bioquímica y Biología MolecularSociedad Argentina de InmunologíaSociedad Argentina de AndrologiaSociedad Argentina de BiofísicaSociedad Argentina de BiologiaSociedad Argentina de Farmacología ExperimentalSociedad Argentina de FisiologiaSociedad Argentina de HematologiaSociedad Argentina de ProtozoologiaFundación Revista Medicina2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/157343Altering microtubule polymerization in Sertoli cells strongly affects their lipid metabolism; LXII Reunión Científica de la Sociedad Argentina de Investigación Clínica; LIII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica ; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de Protozoología; Buenos Aires; Argentina; 2017; 372-3721669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicinaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:39Zoai:ri.conicet.gov.ar:11336/157343instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:39.766CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Altering microtubule polymerization in Sertoli cells strongly affects their lipid metabolism
title Altering microtubule polymerization in Sertoli cells strongly affects their lipid metabolism
spellingShingle Altering microtubule polymerization in Sertoli cells strongly affects their lipid metabolism
Picardi, María Victoria
Nocodazole
mitochondria
lipids
cytoskeleton
title_short Altering microtubule polymerization in Sertoli cells strongly affects their lipid metabolism
title_full Altering microtubule polymerization in Sertoli cells strongly affects their lipid metabolism
title_fullStr Altering microtubule polymerization in Sertoli cells strongly affects their lipid metabolism
title_full_unstemmed Altering microtubule polymerization in Sertoli cells strongly affects their lipid metabolism
title_sort Altering microtubule polymerization in Sertoli cells strongly affects their lipid metabolism
dc.creator.none.fl_str_mv Picardi, María Victoria
Furland, Natalia Edith
Aveldaño, Marta Isabel
Valles, Ana Sofia
author Picardi, María Victoria
author_facet Picardi, María Victoria
Furland, Natalia Edith
Aveldaño, Marta Isabel
Valles, Ana Sofia
author_role author
author2 Furland, Natalia Edith
Aveldaño, Marta Isabel
Valles, Ana Sofia
author2_role author
author
author
dc.subject.none.fl_str_mv Nocodazole
mitochondria
lipids
cytoskeleton
topic Nocodazole
mitochondria
lipids
cytoskeleton
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this work was to study the biological consequences of altering microtubule polymerization in Sertoli cells, with a special focus on their lipid metabolism. For this purpose, the effect of longterm exposures to nocodazole (NCZ) (0.01 µM) was studied on TM4 cells in culture. In addition to the expected effect of NCZ on the depolymerization of tubulin microtubules, a remarkable reorganization was observed in the intermediate flament network (vimentin), which showed a more peripheral distribution than in untreated cells. As compared to cells cultured in control conditions, those exposed to NCZ displayed an increased mitochondrial membrane depolarization (p<0.001). Their plasma membrane integrity was concomitantly altered, as shown by a considerable increase in the leakage of lactate dehydrogenase (p<0.01) released into the culture medium. In addition, the cell proliferation was signifcantly reduced (p<0.003) in NCZ-exposed cells. That the drug induced a major alteration on lipid biosynthesis was clearly apparent from a signifcant increase in triacylglycerol (TAG) levels (p<0.0002) and a massive accumulation of lipid droplets in the cytoplasm of cells. This observation was sustained by following the incorporation and distribution of labeled arachidonic acid ([3H]AA). After 1 hour of incubation, the most highly [3H]-labeled lipid class were TAG in control cells, but choline glycerophospholipids in drug-treated cells. Interestingly 72 hours later, the percentages of label in TAG and cholesterol esters were about twice higher in NCZ-treated than in control cells. We conclude that the cytoskeletal alterations that Sertoli cells undergo after being exposed to NCZ, through their impact on mitochondrial functions and cytoplasmic architecture, are closely involved in the deranged lipid metabolism induced by the drug.
Fil: Picardi, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LXII Reunión Científica de la Sociedad Argentina de Investigación Clínica; LIII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica ; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de Protozoología
Buenos Aires
Argentina
Sociedad Argentina de Investigacion Clinica
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Sociedad Argentina de Inmunología
Sociedad Argentina de Andrologia
Sociedad Argentina de Biofísica
Sociedad Argentina de Biologia
Sociedad Argentina de Farmacología Experimental
Sociedad Argentina de Fisiologia
Sociedad Argentina de Hematologia
Sociedad Argentina de Protozoologia
description The aim of this work was to study the biological consequences of altering microtubule polymerization in Sertoli cells, with a special focus on their lipid metabolism. For this purpose, the effect of longterm exposures to nocodazole (NCZ) (0.01 µM) was studied on TM4 cells in culture. In addition to the expected effect of NCZ on the depolymerization of tubulin microtubules, a remarkable reorganization was observed in the intermediate flament network (vimentin), which showed a more peripheral distribution than in untreated cells. As compared to cells cultured in control conditions, those exposed to NCZ displayed an increased mitochondrial membrane depolarization (p<0.001). Their plasma membrane integrity was concomitantly altered, as shown by a considerable increase in the leakage of lactate dehydrogenase (p<0.01) released into the culture medium. In addition, the cell proliferation was signifcantly reduced (p<0.003) in NCZ-exposed cells. That the drug induced a major alteration on lipid biosynthesis was clearly apparent from a signifcant increase in triacylglycerol (TAG) levels (p<0.0002) and a massive accumulation of lipid droplets in the cytoplasm of cells. This observation was sustained by following the incorporation and distribution of labeled arachidonic acid ([3H]AA). After 1 hour of incubation, the most highly [3H]-labeled lipid class were TAG in control cells, but choline glycerophospholipids in drug-treated cells. Interestingly 72 hours later, the percentages of label in TAG and cholesterol esters were about twice higher in NCZ-treated than in control cells. We conclude that the cytoskeletal alterations that Sertoli cells undergo after being exposed to NCZ, through their impact on mitochondrial functions and cytoplasmic architecture, are closely involved in the deranged lipid metabolism induced by the drug.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/157343
Altering microtubule polymerization in Sertoli cells strongly affects their lipid metabolism; LXII Reunión Científica de la Sociedad Argentina de Investigación Clínica; LIII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica ; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de Protozoología; Buenos Aires; Argentina; 2017; 372-372
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/157343
identifier_str_mv Altering microtubule polymerization in Sertoli cells strongly affects their lipid metabolism; LXII Reunión Científica de la Sociedad Argentina de Investigación Clínica; LIII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica ; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de Protozoología; Buenos Aires; Argentina; 2017; 372-372
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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