GHEP-ISFG collaborative exercise on mixture profiles of autosomal STRs (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03): results and evaluation

Autores
Sala, Adriana Andrea; Crespillo, M.; Barrio, P. A.; Luque, J. A.; Alves, Cíntia; Aler, M.; Alessandrini, F.; Andrade, L.; Barretto, R. M.; Bofarull, A.; Costa, S.; García, M. A.; García, O.; Gaviria, A.; Gladys, A.; Gorostiza, A.; Hernández, A.; Herrera, M.; Hombreiro, L.; Ibarra, A. A.; Jiménez, M. J.; Luque, G. M.; Madero, P.; Martínez Jarreta, B.; Masciovecchio, M. Verónica; Modesti, Nidia Maria; Moreno, F.; Pagano, S.; Pedrosa, S.; Plaza, G.; Prat, E.; Puente, J.; Rendo, F.; Ribeiro, T.; Santamaría, E.; Saragoni, V. G.; Whittle, M. R.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
One of the main objectives of the Spanish and Portuguese-Speaking Group of the International Society for Forensic Genetics (GHEP-ISFG) is to promote and contribute to the development and dissemination of scientific knowledge in the area of forensic genetics. Due to this fact, GHEP-ISFG holds different working commissions that are set up to develop activities in scientific aspects of general interest. One of them, the Mixture Commission of GHEP-ISFG, has organized annually, since 2009, a collaborative exercise on analysis and interpretation of autosomal short tandem repeat (STR) mixture profiles. Until now, three exercises have been organized (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03), with 32, 24 and 17 participant laboratories respectively. The exercise aims to give a general vision by addressing, through the proposal of mock cases, aspects related to the edition of mixture profiles and the statistical treatment. The main conclusions obtained from these exercises may be summarized as follows. Firstly, the data show an increased tendency of the laboratories toward validation of DNA mixture profiles analysis following international recommendations (ISO/IEC 17025:2005). Secondly, the majority of discrepancies are mainly encountered in stutters positions (53.4%, 96.0% and 74.9%, respectively for the three editions). On the other hand, the results submitted reveal the importance of performing duplicate analysis by using different kits in order to reduce errors as much as possible. Regarding the statistical aspect (GHEP-MIX02 and 03), all participants employed the likelihood ratio (LR) parameter to evaluate the statistical compatibility and the formulas employed were quite similar. When the hypotheses to evaluate the LR value were locked by the coordinators (GHEP-MIX02) the results revealed a minor number of discrepancies that were mainly due to clerical reasons. However, the GHEP-MIX03 exercise allowed the participants to freely come up with their own hypotheses to calculate the LR value. In this situation the laboratories reported several options to explain the mock cases proposed and therefore significant differences between the final LR values were obtained. Complete information concerning the background of the criminal case is a critical aspect in order to select the adequate hypotheses to calculate the LR value. Although this should be a task for the judicial court to decide, it is important for the expert to account for the different possibilities and scenarios, and also offer this expertise to the judge. In addition, continuing education in the analysis and interpretation of mixture DNA profiles may also be a priority for the vast majority of forensic laboratories.
Fil: Sala, Adriana Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Crespillo, M.. Instituto Nacional de Toxicología y Ciencias Forenses; España
Fil: Barrio, P. A.. Instituto Nacional de Toxicología y Ciencias Forenses; España
Fil: Luque, J. A.. Instituto Nacional de Toxicología y Ciencias Forenses; España
Fil: Alves, Cíntia. Universidad de Porto; Portugal
Fil: Aler, M.. Servicio de Laboratorio. Sección de Genética Forense y Criminalística; España
Fil: Alessandrini, F.. Università Politecnica delle Marche. Department of Biomedical Sciences and Public Health; Italia
Fil: Andrade, L.. Instituto Nacional de Medicina Legal e Ciências Forenses, Delegação do Centro. Serviço de Genética e Biologia Forenses; Portugal
Fil: Barretto, R. M.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Bofarull, A.. Instituto Nacional de Toxicología y Ciencias Forenses; España
Fil: Costa, S.. Instituto Nacional de Medicina Legal y Ciencias Forenses; Portugal
Fil: García, M. A.. Servicio de Criminalística de la Guardia Civil. Laboratorio Central de Criminalística. Departamento de Biología; España
Fil: García, O.. Basque Country Police. Forensic Genetics Section. Forensic Science Unit; España
Fil: Gaviria, A.. Cruz Roja Ecuatoriana. Laboratorio de Genética Molecular; Ecuador
Fil: Gladys, A.. Corte Suprema de Justicia de la Nación; Argentina
Fil: Gorostiza, A.. Grupo Zeltia. Genomica S. A. U.. Laboratorio de Identificación Genética; España
Fil: Hernández, A.. Instituto Nacional de Toxicología y Ciencias Forenses; España
Fil: Herrera, M.. Laboratorio Genda S. A.; Argentina
Fil: Hombreiro, L.. Jefatura Superior de Policía de Galicia. Brigada de Policía Científica. Laboratorio Territorial de Biología – ADN; España
Fil: Ibarra, A. A.. Universidad de Antioquia; Colombia
Fil: Jiménez, M. J.. Policia de la Generalitat – Mossos d’Esquadra. Divisió de Policia Científica. Àrea Central de Criminalística. Unitat Central de Laboratori Biològic; España
Fil: Luque, G. M.. Instituto Nacional de Toxicología y Ciencias Forenses; España
Fil: Madero, P.. Centro de Análisis Genéticos; España
Fil: Martínez Jarreta, B.. Universidad de Zaragoza; España
Fil: Masciovecchio, M. Verónica. IACA Laboratorios; Argentina
Fil: Modesti, Nidia Maria. Provincia de Córdoba. Poder Judicial; Argentina
Fil: Moreno, F.. Servicio Médico Legal. Unidad de Genética Forense; Chile
Fil: Pagano, S.. Dirección Nacional de Policía Técnica. Laboratorio de Análisis de ADN para el CODIS; Uruguay
Fil: Pedrosa, S.. Navarra de Servicios y Tecnologías S. A. U.; España
Fil: Plaza, G.. Neodiagnostica S. L.; España
Fil: Prat, E.. Comisaría General de Policía Científica. Laboratorio de ADN; España
Fil: Puente, J.. Laboratorio de Genética Clínica S. L.; España
Fil: Rendo, F.. Universidad del País Vasco; España
Fil: Ribeiro, T.. Instituto Nacional de Medicina Legal e Ciências Forenses, Delegação Sul. Serviço de Genética e Biologia Forenses; Portugal
Fil: Santamaría, E.. Instituto Nacional de Toxicología y Ciencias Forenses; España
Fil: Saragoni, V. G.. Servicio Médico Legal. Departamento de Laboratorios. Unidad de Genética Forense; Chile
Fil: Whittle, M. R.. Genomic Engenharia Molecular; Brasil
Materia
Dna Analysis
Mixture Interpretation
Autosomal Strs
Collaborative Exercise
Spanish And Portuguese-Speaking Working Group
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16207
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/16207
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling GHEP-ISFG collaborative exercise on mixture profiles of autosomal STRs (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03): results and evaluationSala, Adriana AndreaCrespillo, M.Barrio, P. A.Luque, J. A.Alves, CíntiaAler, M.Alessandrini, F.Andrade, L.Barretto, R. M.Bofarull, A.Costa, S.García, M. A.García, O.Gaviria, A.Gladys, A.Gorostiza, A.Hernández, A.Herrera, M.Hombreiro, L.Ibarra, A. A.Jiménez, M. J.Luque, G. M.Madero, P.Martínez Jarreta, B.Masciovecchio, M. VerónicaModesti, Nidia MariaMoreno, F.Pagano, S.Pedrosa, S.Plaza, G.Prat, E.Puente, J.Rendo, F.Ribeiro, T.Santamaría, E.Saragoni, V. G.Whittle, M. R.Dna AnalysisMixture InterpretationAutosomal StrsCollaborative ExerciseSpanish And Portuguese-Speaking Working Grouphttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3One of the main objectives of the Spanish and Portuguese-Speaking Group of the International Society for Forensic Genetics (GHEP-ISFG) is to promote and contribute to the development and dissemination of scientific knowledge in the area of forensic genetics. Due to this fact, GHEP-ISFG holds different working commissions that are set up to develop activities in scientific aspects of general interest. One of them, the Mixture Commission of GHEP-ISFG, has organized annually, since 2009, a collaborative exercise on analysis and interpretation of autosomal short tandem repeat (STR) mixture profiles. Until now, three exercises have been organized (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03), with 32, 24 and 17 participant laboratories respectively. The exercise aims to give a general vision by addressing, through the proposal of mock cases, aspects related to the edition of mixture profiles and the statistical treatment. The main conclusions obtained from these exercises may be summarized as follows. Firstly, the data show an increased tendency of the laboratories toward validation of DNA mixture profiles analysis following international recommendations (ISO/IEC 17025:2005). Secondly, the majority of discrepancies are mainly encountered in stutters positions (53.4%, 96.0% and 74.9%, respectively for the three editions). On the other hand, the results submitted reveal the importance of performing duplicate analysis by using different kits in order to reduce errors as much as possible. Regarding the statistical aspect (GHEP-MIX02 and 03), all participants employed the likelihood ratio (LR) parameter to evaluate the statistical compatibility and the formulas employed were quite similar. When the hypotheses to evaluate the LR value were locked by the coordinators (GHEP-MIX02) the results revealed a minor number of discrepancies that were mainly due to clerical reasons. However, the GHEP-MIX03 exercise allowed the participants to freely come up with their own hypotheses to calculate the LR value. In this situation the laboratories reported several options to explain the mock cases proposed and therefore significant differences between the final LR values were obtained. Complete information concerning the background of the criminal case is a critical aspect in order to select the adequate hypotheses to calculate the LR value. Although this should be a task for the judicial court to decide, it is important for the expert to account for the different possibilities and scenarios, and also offer this expertise to the judge. In addition, continuing education in the analysis and interpretation of mixture DNA profiles may also be a priority for the vast majority of forensic laboratories.Fil: Sala, Adriana Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Crespillo, M.. Instituto Nacional de Toxicología y Ciencias Forenses; EspañaFil: Barrio, P. A.. Instituto Nacional de Toxicología y Ciencias Forenses; EspañaFil: Luque, J. A.. Instituto Nacional de Toxicología y Ciencias Forenses; EspañaFil: Alves, Cíntia. Universidad de Porto; PortugalFil: Aler, M.. Servicio de Laboratorio. Sección de Genética Forense y Criminalística; EspañaFil: Alessandrini, F.. Università Politecnica delle Marche. Department of Biomedical Sciences and Public Health; ItaliaFil: Andrade, L.. Instituto Nacional de Medicina Legal e Ciências Forenses, Delegação do Centro. Serviço de Genética e Biologia Forenses; PortugalFil: Barretto, R. M.. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Bofarull, A.. Instituto Nacional de Toxicología y Ciencias Forenses; EspañaFil: Costa, S.. Instituto Nacional de Medicina Legal y Ciencias Forenses; PortugalFil: García, M. A.. Servicio de Criminalística de la Guardia Civil. Laboratorio Central de Criminalística. Departamento de Biología; EspañaFil: García, O.. Basque Country Police. Forensic Genetics Section. Forensic Science Unit; EspañaFil: Gaviria, A.. Cruz Roja Ecuatoriana. Laboratorio de Genética Molecular; EcuadorFil: Gladys, A.. Corte Suprema de Justicia de la Nación; ArgentinaFil: Gorostiza, A.. Grupo Zeltia. Genomica S. A. U.. Laboratorio de Identificación Genética; EspañaFil: Hernández, A.. Instituto Nacional de Toxicología y Ciencias Forenses; EspañaFil: Herrera, M.. Laboratorio Genda S. A.; ArgentinaFil: Hombreiro, L.. Jefatura Superior de Policía de Galicia. Brigada de Policía Científica. Laboratorio Territorial de Biología – ADN; EspañaFil: Ibarra, A. A.. Universidad de Antioquia; ColombiaFil: Jiménez, M. J.. Policia de la Generalitat – Mossos d’Esquadra. Divisió de Policia Científica. Àrea Central de Criminalística. Unitat Central de Laboratori Biològic; EspañaFil: Luque, G. M.. Instituto Nacional de Toxicología y Ciencias Forenses; EspañaFil: Madero, P.. Centro de Análisis Genéticos; EspañaFil: Martínez Jarreta, B.. Universidad de Zaragoza; EspañaFil: Masciovecchio, M. Verónica. IACA Laboratorios; ArgentinaFil: Modesti, Nidia Maria. Provincia de Córdoba. Poder Judicial; ArgentinaFil: Moreno, F.. Servicio Médico Legal. Unidad de Genética Forense; ChileFil: Pagano, S.. Dirección Nacional de Policía Técnica. Laboratorio de Análisis de ADN para el CODIS; UruguayFil: Pedrosa, S.. Navarra de Servicios y Tecnologías S. A. U.; EspañaFil: Plaza, G.. Neodiagnostica S. L.; EspañaFil: Prat, E.. Comisaría General de Policía Científica. Laboratorio de ADN; EspañaFil: Puente, J.. Laboratorio de Genética Clínica S. L.; EspañaFil: Rendo, F.. Universidad del País Vasco; EspañaFil: Ribeiro, T.. Instituto Nacional de Medicina Legal e Ciências Forenses, Delegação Sul. Serviço de Genética e Biologia Forenses; PortugalFil: Santamaría, E.. Instituto Nacional de Toxicología y Ciencias Forenses; EspañaFil: Saragoni, V. G.. Servicio Médico Legal. Departamento de Laboratorios. Unidad de Genética Forense; ChileFil: Whittle, M. R.. Genomic Engenharia Molecular; BrasilElsevier Ireland2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16207Sala, Adriana Andrea; Crespillo, M.; Barrio, P. A.; Luque, J. A.; Alves, Cíntia; et al.; GHEP-ISFG collaborative exercise on mixture profiles of autosomal STRs (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03): results and evaluation; Elsevier Ireland; Forensic Science International: Genetics; 10; 2-2014; 64-721872-4973enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsigen.2014.01.009info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1872497314000258info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:54:35Zoai:ri.conicet.gov.ar:11336/16207instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:54:35.394CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv GHEP-ISFG collaborative exercise on mixture profiles of autosomal STRs (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03): results and evaluation
title GHEP-ISFG collaborative exercise on mixture profiles of autosomal STRs (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03): results and evaluation
spellingShingle GHEP-ISFG collaborative exercise on mixture profiles of autosomal STRs (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03): results and evaluation
Sala, Adriana Andrea
Dna Analysis
Mixture Interpretation
Autosomal Strs
Collaborative Exercise
Spanish And Portuguese-Speaking Working Group
title_short GHEP-ISFG collaborative exercise on mixture profiles of autosomal STRs (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03): results and evaluation
title_full GHEP-ISFG collaborative exercise on mixture profiles of autosomal STRs (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03): results and evaluation
title_fullStr GHEP-ISFG collaborative exercise on mixture profiles of autosomal STRs (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03): results and evaluation
title_full_unstemmed GHEP-ISFG collaborative exercise on mixture profiles of autosomal STRs (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03): results and evaluation
title_sort GHEP-ISFG collaborative exercise on mixture profiles of autosomal STRs (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03): results and evaluation
dc.creator.none.fl_str_mv Sala, Adriana Andrea
Crespillo, M.
Barrio, P. A.
Luque, J. A.
Alves, Cíntia
Aler, M.
Alessandrini, F.
Andrade, L.
Barretto, R. M.
Bofarull, A.
Costa, S.
García, M. A.
García, O.
Gaviria, A.
Gladys, A.
Gorostiza, A.
Hernández, A.
Herrera, M.
Hombreiro, L.
Ibarra, A. A.
Jiménez, M. J.
Luque, G. M.
Madero, P.
Martínez Jarreta, B.
Masciovecchio, M. Verónica
Modesti, Nidia Maria
Moreno, F.
Pagano, S.
Pedrosa, S.
Plaza, G.
Prat, E.
Puente, J.
Rendo, F.
Ribeiro, T.
Santamaría, E.
Saragoni, V. G.
Whittle, M. R.
author Sala, Adriana Andrea
author_facet Sala, Adriana Andrea
Crespillo, M.
Barrio, P. A.
Luque, J. A.
Alves, Cíntia
Aler, M.
Alessandrini, F.
Andrade, L.
Barretto, R. M.
Bofarull, A.
Costa, S.
García, M. A.
García, O.
Gaviria, A.
Gladys, A.
Gorostiza, A.
Hernández, A.
Herrera, M.
Hombreiro, L.
Ibarra, A. A.
Jiménez, M. J.
Luque, G. M.
Madero, P.
Martínez Jarreta, B.
Masciovecchio, M. Verónica
Modesti, Nidia Maria
Moreno, F.
Pagano, S.
Pedrosa, S.
Plaza, G.
Prat, E.
Puente, J.
Rendo, F.
Ribeiro, T.
Santamaría, E.
Saragoni, V. G.
Whittle, M. R.
author_role author
author2 Crespillo, M.
Barrio, P. A.
Luque, J. A.
Alves, Cíntia
Aler, M.
Alessandrini, F.
Andrade, L.
Barretto, R. M.
Bofarull, A.
Costa, S.
García, M. A.
García, O.
Gaviria, A.
Gladys, A.
Gorostiza, A.
Hernández, A.
Herrera, M.
Hombreiro, L.
Ibarra, A. A.
Jiménez, M. J.
Luque, G. M.
Madero, P.
Martínez Jarreta, B.
Masciovecchio, M. Verónica
Modesti, Nidia Maria
Moreno, F.
Pagano, S.
Pedrosa, S.
Plaza, G.
Prat, E.
Puente, J.
Rendo, F.
Ribeiro, T.
Santamaría, E.
Saragoni, V. G.
Whittle, M. R.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Dna Analysis
Mixture Interpretation
Autosomal Strs
Collaborative Exercise
Spanish And Portuguese-Speaking Working Group
topic Dna Analysis
Mixture Interpretation
Autosomal Strs
Collaborative Exercise
Spanish And Portuguese-Speaking Working Group
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv One of the main objectives of the Spanish and Portuguese-Speaking Group of the International Society for Forensic Genetics (GHEP-ISFG) is to promote and contribute to the development and dissemination of scientific knowledge in the area of forensic genetics. Due to this fact, GHEP-ISFG holds different working commissions that are set up to develop activities in scientific aspects of general interest. One of them, the Mixture Commission of GHEP-ISFG, has organized annually, since 2009, a collaborative exercise on analysis and interpretation of autosomal short tandem repeat (STR) mixture profiles. Until now, three exercises have been organized (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03), with 32, 24 and 17 participant laboratories respectively. The exercise aims to give a general vision by addressing, through the proposal of mock cases, aspects related to the edition of mixture profiles and the statistical treatment. The main conclusions obtained from these exercises may be summarized as follows. Firstly, the data show an increased tendency of the laboratories toward validation of DNA mixture profiles analysis following international recommendations (ISO/IEC 17025:2005). Secondly, the majority of discrepancies are mainly encountered in stutters positions (53.4%, 96.0% and 74.9%, respectively for the three editions). On the other hand, the results submitted reveal the importance of performing duplicate analysis by using different kits in order to reduce errors as much as possible. Regarding the statistical aspect (GHEP-MIX02 and 03), all participants employed the likelihood ratio (LR) parameter to evaluate the statistical compatibility and the formulas employed were quite similar. When the hypotheses to evaluate the LR value were locked by the coordinators (GHEP-MIX02) the results revealed a minor number of discrepancies that were mainly due to clerical reasons. However, the GHEP-MIX03 exercise allowed the participants to freely come up with their own hypotheses to calculate the LR value. In this situation the laboratories reported several options to explain the mock cases proposed and therefore significant differences between the final LR values were obtained. Complete information concerning the background of the criminal case is a critical aspect in order to select the adequate hypotheses to calculate the LR value. Although this should be a task for the judicial court to decide, it is important for the expert to account for the different possibilities and scenarios, and also offer this expertise to the judge. In addition, continuing education in the analysis and interpretation of mixture DNA profiles may also be a priority for the vast majority of forensic laboratories.
Fil: Sala, Adriana Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Crespillo, M.. Instituto Nacional de Toxicología y Ciencias Forenses; España
Fil: Barrio, P. A.. Instituto Nacional de Toxicología y Ciencias Forenses; España
Fil: Luque, J. A.. Instituto Nacional de Toxicología y Ciencias Forenses; España
Fil: Alves, Cíntia. Universidad de Porto; Portugal
Fil: Aler, M.. Servicio de Laboratorio. Sección de Genética Forense y Criminalística; España
Fil: Alessandrini, F.. Università Politecnica delle Marche. Department of Biomedical Sciences and Public Health; Italia
Fil: Andrade, L.. Instituto Nacional de Medicina Legal e Ciências Forenses, Delegação do Centro. Serviço de Genética e Biologia Forenses; Portugal
Fil: Barretto, R. M.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Bofarull, A.. Instituto Nacional de Toxicología y Ciencias Forenses; España
Fil: Costa, S.. Instituto Nacional de Medicina Legal y Ciencias Forenses; Portugal
Fil: García, M. A.. Servicio de Criminalística de la Guardia Civil. Laboratorio Central de Criminalística. Departamento de Biología; España
Fil: García, O.. Basque Country Police. Forensic Genetics Section. Forensic Science Unit; España
Fil: Gaviria, A.. Cruz Roja Ecuatoriana. Laboratorio de Genética Molecular; Ecuador
Fil: Gladys, A.. Corte Suprema de Justicia de la Nación; Argentina
Fil: Gorostiza, A.. Grupo Zeltia. Genomica S. A. U.. Laboratorio de Identificación Genética; España
Fil: Hernández, A.. Instituto Nacional de Toxicología y Ciencias Forenses; España
Fil: Herrera, M.. Laboratorio Genda S. A.; Argentina
Fil: Hombreiro, L.. Jefatura Superior de Policía de Galicia. Brigada de Policía Científica. Laboratorio Territorial de Biología – ADN; España
Fil: Ibarra, A. A.. Universidad de Antioquia; Colombia
Fil: Jiménez, M. J.. Policia de la Generalitat – Mossos d’Esquadra. Divisió de Policia Científica. Àrea Central de Criminalística. Unitat Central de Laboratori Biològic; España
Fil: Luque, G. M.. Instituto Nacional de Toxicología y Ciencias Forenses; España
Fil: Madero, P.. Centro de Análisis Genéticos; España
Fil: Martínez Jarreta, B.. Universidad de Zaragoza; España
Fil: Masciovecchio, M. Verónica. IACA Laboratorios; Argentina
Fil: Modesti, Nidia Maria. Provincia de Córdoba. Poder Judicial; Argentina
Fil: Moreno, F.. Servicio Médico Legal. Unidad de Genética Forense; Chile
Fil: Pagano, S.. Dirección Nacional de Policía Técnica. Laboratorio de Análisis de ADN para el CODIS; Uruguay
Fil: Pedrosa, S.. Navarra de Servicios y Tecnologías S. A. U.; España
Fil: Plaza, G.. Neodiagnostica S. L.; España
Fil: Prat, E.. Comisaría General de Policía Científica. Laboratorio de ADN; España
Fil: Puente, J.. Laboratorio de Genética Clínica S. L.; España
Fil: Rendo, F.. Universidad del País Vasco; España
Fil: Ribeiro, T.. Instituto Nacional de Medicina Legal e Ciências Forenses, Delegação Sul. Serviço de Genética e Biologia Forenses; Portugal
Fil: Santamaría, E.. Instituto Nacional de Toxicología y Ciencias Forenses; España
Fil: Saragoni, V. G.. Servicio Médico Legal. Departamento de Laboratorios. Unidad de Genética Forense; Chile
Fil: Whittle, M. R.. Genomic Engenharia Molecular; Brasil
description One of the main objectives of the Spanish and Portuguese-Speaking Group of the International Society for Forensic Genetics (GHEP-ISFG) is to promote and contribute to the development and dissemination of scientific knowledge in the area of forensic genetics. Due to this fact, GHEP-ISFG holds different working commissions that are set up to develop activities in scientific aspects of general interest. One of them, the Mixture Commission of GHEP-ISFG, has organized annually, since 2009, a collaborative exercise on analysis and interpretation of autosomal short tandem repeat (STR) mixture profiles. Until now, three exercises have been organized (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03), with 32, 24 and 17 participant laboratories respectively. The exercise aims to give a general vision by addressing, through the proposal of mock cases, aspects related to the edition of mixture profiles and the statistical treatment. The main conclusions obtained from these exercises may be summarized as follows. Firstly, the data show an increased tendency of the laboratories toward validation of DNA mixture profiles analysis following international recommendations (ISO/IEC 17025:2005). Secondly, the majority of discrepancies are mainly encountered in stutters positions (53.4%, 96.0% and 74.9%, respectively for the three editions). On the other hand, the results submitted reveal the importance of performing duplicate analysis by using different kits in order to reduce errors as much as possible. Regarding the statistical aspect (GHEP-MIX02 and 03), all participants employed the likelihood ratio (LR) parameter to evaluate the statistical compatibility and the formulas employed were quite similar. When the hypotheses to evaluate the LR value were locked by the coordinators (GHEP-MIX02) the results revealed a minor number of discrepancies that were mainly due to clerical reasons. However, the GHEP-MIX03 exercise allowed the participants to freely come up with their own hypotheses to calculate the LR value. In this situation the laboratories reported several options to explain the mock cases proposed and therefore significant differences between the final LR values were obtained. Complete information concerning the background of the criminal case is a critical aspect in order to select the adequate hypotheses to calculate the LR value. Although this should be a task for the judicial court to decide, it is important for the expert to account for the different possibilities and scenarios, and also offer this expertise to the judge. In addition, continuing education in the analysis and interpretation of mixture DNA profiles may also be a priority for the vast majority of forensic laboratories.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16207
Sala, Adriana Andrea; Crespillo, M.; Barrio, P. A.; Luque, J. A.; Alves, Cíntia; et al.; GHEP-ISFG collaborative exercise on mixture profiles of autosomal STRs (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03): results and evaluation; Elsevier Ireland; Forensic Science International: Genetics; 10; 2-2014; 64-72
1872-4973
url http://hdl.handle.net/11336/16207
identifier_str_mv Sala, Adriana Andrea; Crespillo, M.; Barrio, P. A.; Luque, J. A.; Alves, Cíntia; et al.; GHEP-ISFG collaborative exercise on mixture profiles of autosomal STRs (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03): results and evaluation; Elsevier Ireland; Forensic Science International: Genetics; 10; 2-2014; 64-72
1872-4973
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsigen.2014.01.009
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1872497314000258
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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