Torque Teno Virus (TTV) in Renal Transplant Recipients: Species Diversity and Variability

Autores
Reyes, Noelia Soledad; Spezia, Pietro Giorgio; Jara, Ana Raquel; Filippini, Fabio; Boccia, Natalia; García, Gonzalo; Hermida, Eliana Micaela; Poletta, Fernando Adrián; Pistello, Mauro; Laham, Gustavo; Maggi, Fabrizio; Echavarría, Marcela Silvia
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Torque Teno Virus (TTV) is a nonpathogenic and ubiquitous ssDNA virus, a member of the Anelloviridae family. TTV has been postulated as a biomarker in transplant patients. This study aimed to determine the TTV species diversity and variability in renal transplant recipients and to associate species diversity with the corresponding TTV viral load. From 27 recipients, 30 plasma samples were selected. Viral load was determined using two real-time PCR assays, followed by RCA-NGS and ORF1 phylogenetic analysis. The TTV diversity was determined in all samples. Variability was determined in three patients with two sequential samples (pre- and post-transplantation). Most of the samples presented multiple TTV species, up to 15 different species were detected. In the pre-transplant samples (n = 12), the most prevalent species were TTV3 (75%) and TTV13 (75%), and the median number of species per sample was 5 (IQR: 4–7.5). TTV3 was also the most prevalent (56%) in the post-transplant samples (n = 18), and the median number of species was 2 (IQR: 1.8–5.5). No significant correlation between the number of species and viral load was found. The number and type of TTV species showed total variability over time. We report high TTV species diversity in Argentinian recipients, especially in pre-transplant period, with total intra-host variability. However, we found no significant correlation between this high diversity and TTV viral load.
Fil: Reyes, Noelia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina
Fil: Spezia, Pietro Giorgio. National Institute for Infectious Diseases Lazzaro Spallanzani; Italia
Fil: Jara, Ana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina
Fil: Filippini, Fabio. Università degli Studi di Pisa; Italia
Fil: Boccia, Natalia. Centro de Educación Médica e Investigaciones Clínicas "Norberto Quirno"; Argentina
Fil: García, Gonzalo. Centro de Educación Médica e Investigaciones Clínicas "Norberto Quirno"; Argentina
Fil: Hermida, Eliana Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina
Fil: Poletta, Fernando Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina
Fil: Pistello, Mauro. Università degli Studi di Pisa; Italia
Fil: Laham, Gustavo. Centro de Educación Médica e Investigaciones Clínicas "Norberto Quirno"; Argentina
Fil: Maggi, Fabrizio. National Institute for Infectious Diseases Lazzaro Spallanzani; Italia
Fil: Echavarría, Marcela Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina
Materia
Torque Teno Virus
TTV species
Renal transplantation
Anelloviruses
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/267801
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/267801
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Torque Teno Virus (TTV) in Renal Transplant Recipients: Species Diversity and VariabilityReyes, Noelia SoledadSpezia, Pietro GiorgioJara, Ana RaquelFilippini, FabioBoccia, NataliaGarcía, GonzaloHermida, Eliana MicaelaPoletta, Fernando AdriánPistello, MauroLaham, GustavoMaggi, FabrizioEchavarría, Marcela SilviaTorque Teno VirusTTV speciesRenal transplantationAnelloviruseshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Torque Teno Virus (TTV) is a nonpathogenic and ubiquitous ssDNA virus, a member of the Anelloviridae family. TTV has been postulated as a biomarker in transplant patients. This study aimed to determine the TTV species diversity and variability in renal transplant recipients and to associate species diversity with the corresponding TTV viral load. From 27 recipients, 30 plasma samples were selected. Viral load was determined using two real-time PCR assays, followed by RCA-NGS and ORF1 phylogenetic analysis. The TTV diversity was determined in all samples. Variability was determined in three patients with two sequential samples (pre- and post-transplantation). Most of the samples presented multiple TTV species, up to 15 different species were detected. In the pre-transplant samples (n = 12), the most prevalent species were TTV3 (75%) and TTV13 (75%), and the median number of species per sample was 5 (IQR: 4–7.5). TTV3 was also the most prevalent (56%) in the post-transplant samples (n = 18), and the median number of species was 2 (IQR: 1.8–5.5). No significant correlation between the number of species and viral load was found. The number and type of TTV species showed total variability over time. We report high TTV species diversity in Argentinian recipients, especially in pre-transplant period, with total intra-host variability. However, we found no significant correlation between this high diversity and TTV viral load.Fil: Reyes, Noelia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; ArgentinaFil: Spezia, Pietro Giorgio. National Institute for Infectious Diseases Lazzaro Spallanzani; ItaliaFil: Jara, Ana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; ArgentinaFil: Filippini, Fabio. Università degli Studi di Pisa; ItaliaFil: Boccia, Natalia. Centro de Educación Médica e Investigaciones Clínicas "Norberto Quirno"; ArgentinaFil: García, Gonzalo. Centro de Educación Médica e Investigaciones Clínicas "Norberto Quirno"; ArgentinaFil: Hermida, Eliana Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; ArgentinaFil: Poletta, Fernando Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; ArgentinaFil: Pistello, Mauro. Università degli Studi di Pisa; ItaliaFil: Laham, Gustavo. Centro de Educación Médica e Investigaciones Clínicas "Norberto Quirno"; ArgentinaFil: Maggi, Fabrizio. National Institute for Infectious Diseases Lazzaro Spallanzani; ItaliaFil: Echavarría, Marcela Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; ArgentinaMultidisciplinary Digital Publishing Institute2024-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/267801Reyes, Noelia Soledad; Spezia, Pietro Giorgio; Jara, Ana Raquel; Filippini, Fabio; Boccia, Natalia; et al.; Torque Teno Virus (TTV) in Renal Transplant Recipients: Species Diversity and Variability; Multidisciplinary Digital Publishing Institute; Viruses; 16; 3; 3-2024; 1-141999-4915CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4915/16/3/432info:eu-repo/semantics/altIdentifier/doi/10.3390/v16030432info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:46Zoai:ri.conicet.gov.ar:11336/267801instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:46.823CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Torque Teno Virus (TTV) in Renal Transplant Recipients: Species Diversity and Variability
title Torque Teno Virus (TTV) in Renal Transplant Recipients: Species Diversity and Variability
spellingShingle Torque Teno Virus (TTV) in Renal Transplant Recipients: Species Diversity and Variability
Reyes, Noelia Soledad
Torque Teno Virus
TTV species
Renal transplantation
Anelloviruses
title_short Torque Teno Virus (TTV) in Renal Transplant Recipients: Species Diversity and Variability
title_full Torque Teno Virus (TTV) in Renal Transplant Recipients: Species Diversity and Variability
title_fullStr Torque Teno Virus (TTV) in Renal Transplant Recipients: Species Diversity and Variability
title_full_unstemmed Torque Teno Virus (TTV) in Renal Transplant Recipients: Species Diversity and Variability
title_sort Torque Teno Virus (TTV) in Renal Transplant Recipients: Species Diversity and Variability
dc.creator.none.fl_str_mv Reyes, Noelia Soledad
Spezia, Pietro Giorgio
Jara, Ana Raquel
Filippini, Fabio
Boccia, Natalia
García, Gonzalo
Hermida, Eliana Micaela
Poletta, Fernando Adrián
Pistello, Mauro
Laham, Gustavo
Maggi, Fabrizio
Echavarría, Marcela Silvia
author Reyes, Noelia Soledad
author_facet Reyes, Noelia Soledad
Spezia, Pietro Giorgio
Jara, Ana Raquel
Filippini, Fabio
Boccia, Natalia
García, Gonzalo
Hermida, Eliana Micaela
Poletta, Fernando Adrián
Pistello, Mauro
Laham, Gustavo
Maggi, Fabrizio
Echavarría, Marcela Silvia
author_role author
author2 Spezia, Pietro Giorgio
Jara, Ana Raquel
Filippini, Fabio
Boccia, Natalia
García, Gonzalo
Hermida, Eliana Micaela
Poletta, Fernando Adrián
Pistello, Mauro
Laham, Gustavo
Maggi, Fabrizio
Echavarría, Marcela Silvia
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Torque Teno Virus
TTV species
Renal transplantation
Anelloviruses
topic Torque Teno Virus
TTV species
Renal transplantation
Anelloviruses
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Torque Teno Virus (TTV) is a nonpathogenic and ubiquitous ssDNA virus, a member of the Anelloviridae family. TTV has been postulated as a biomarker in transplant patients. This study aimed to determine the TTV species diversity and variability in renal transplant recipients and to associate species diversity with the corresponding TTV viral load. From 27 recipients, 30 plasma samples were selected. Viral load was determined using two real-time PCR assays, followed by RCA-NGS and ORF1 phylogenetic analysis. The TTV diversity was determined in all samples. Variability was determined in three patients with two sequential samples (pre- and post-transplantation). Most of the samples presented multiple TTV species, up to 15 different species were detected. In the pre-transplant samples (n = 12), the most prevalent species were TTV3 (75%) and TTV13 (75%), and the median number of species per sample was 5 (IQR: 4–7.5). TTV3 was also the most prevalent (56%) in the post-transplant samples (n = 18), and the median number of species was 2 (IQR: 1.8–5.5). No significant correlation between the number of species and viral load was found. The number and type of TTV species showed total variability over time. We report high TTV species diversity in Argentinian recipients, especially in pre-transplant period, with total intra-host variability. However, we found no significant correlation between this high diversity and TTV viral load.
Fil: Reyes, Noelia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina
Fil: Spezia, Pietro Giorgio. National Institute for Infectious Diseases Lazzaro Spallanzani; Italia
Fil: Jara, Ana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina
Fil: Filippini, Fabio. Università degli Studi di Pisa; Italia
Fil: Boccia, Natalia. Centro de Educación Médica e Investigaciones Clínicas "Norberto Quirno"; Argentina
Fil: García, Gonzalo. Centro de Educación Médica e Investigaciones Clínicas "Norberto Quirno"; Argentina
Fil: Hermida, Eliana Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina
Fil: Poletta, Fernando Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina
Fil: Pistello, Mauro. Università degli Studi di Pisa; Italia
Fil: Laham, Gustavo. Centro de Educación Médica e Investigaciones Clínicas "Norberto Quirno"; Argentina
Fil: Maggi, Fabrizio. National Institute for Infectious Diseases Lazzaro Spallanzani; Italia
Fil: Echavarría, Marcela Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina
description Torque Teno Virus (TTV) is a nonpathogenic and ubiquitous ssDNA virus, a member of the Anelloviridae family. TTV has been postulated as a biomarker in transplant patients. This study aimed to determine the TTV species diversity and variability in renal transplant recipients and to associate species diversity with the corresponding TTV viral load. From 27 recipients, 30 plasma samples were selected. Viral load was determined using two real-time PCR assays, followed by RCA-NGS and ORF1 phylogenetic analysis. The TTV diversity was determined in all samples. Variability was determined in three patients with two sequential samples (pre- and post-transplantation). Most of the samples presented multiple TTV species, up to 15 different species were detected. In the pre-transplant samples (n = 12), the most prevalent species were TTV3 (75%) and TTV13 (75%), and the median number of species per sample was 5 (IQR: 4–7.5). TTV3 was also the most prevalent (56%) in the post-transplant samples (n = 18), and the median number of species was 2 (IQR: 1.8–5.5). No significant correlation between the number of species and viral load was found. The number and type of TTV species showed total variability over time. We report high TTV species diversity in Argentinian recipients, especially in pre-transplant period, with total intra-host variability. However, we found no significant correlation between this high diversity and TTV viral load.
publishDate 2024
dc.date.none.fl_str_mv 2024-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/267801
Reyes, Noelia Soledad; Spezia, Pietro Giorgio; Jara, Ana Raquel; Filippini, Fabio; Boccia, Natalia; et al.; Torque Teno Virus (TTV) in Renal Transplant Recipients: Species Diversity and Variability; Multidisciplinary Digital Publishing Institute; Viruses; 16; 3; 3-2024; 1-14
1999-4915
CONICET Digital
CONICET
url http://hdl.handle.net/11336/267801
identifier_str_mv Reyes, Noelia Soledad; Spezia, Pietro Giorgio; Jara, Ana Raquel; Filippini, Fabio; Boccia, Natalia; et al.; Torque Teno Virus (TTV) in Renal Transplant Recipients: Species Diversity and Variability; Multidisciplinary Digital Publishing Institute; Viruses; 16; 3; 3-2024; 1-14
1999-4915
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4915/16/3/432
info:eu-repo/semantics/altIdentifier/doi/10.3390/v16030432
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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