Attenuated poxviruses expressing a synthetic HIV protein stimulate HLA-A2-restricted cytotoxic T-cell responses

Autores
Didierlaurent, Arnaud; Ramirez, Juan-Carlos; Gherardi, Maria Magdalena; Zimmerli, Simone C.; Graf, Marcus; Orbea, Hans Acha; Pantaleo, Giuseppe; Wagner, Ralf; Esteban, Mariano; Kraehenbuhl, Jean Pierre; Sirard, Jean Claude
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Efficient HIV vaccines have to trigger cell-mediated immunity directed against various viral antigens. However little is known about the breadth of the response induced by vaccines carrying multiple proteins. Here, we report on the immunogenicity of a construct harbouring a fusion of the HIV-1 IIIB gag, pol and nef genes (gpn) designed for optimal safety and equimolar expression of the HIV proteins. The attenuated poxviruses, MVA and NYVAC, harbouring the gpn construct, induced potent immune responses in conventional mice characterised by stimulation of Gpn-specific IFN-γ-producing cells and cytotoxic T cells. In HLA-A2 transgenic mice, recombinant MVA elicited cytotoxic responses against epitopes recognised in most HLA-A2+ HIV-1-infected individuals. We also found that the MVA vaccine triggered the in vitro expansion of peripheral blood cells isolated from a HIV-1-seropositive patient and with similar specificity as found in immunised HLA-A2 transgenic mice. In conclusion, the synthetic HIV polyantigen Gpn delivered by MVA is immunogenic, efficiently processed and presented by human MHC class I molecules.
Fil: Didierlaurent, Arnaud. Universite de Lausanne; Suiza
Fil: Ramirez, Juan-Carlos. Consejo Superior de Investigaciones Científicas; España
Fil: Gherardi, Maria Magdalena. Consejo Superior de Investigaciones Científicas; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zimmerli, Simone C.. No especifíca;
Fil: Graf, Marcus. No especifíca;
Fil: Orbea, Hans Acha. Universite de Lausanne; Suiza
Fil: Pantaleo, Giuseppe. No especifíca;
Fil: Wagner, Ralf. No especifíca;
Fil: Esteban, Mariano. Consejo Superior de Investigaciones Científicas; España
Fil: Kraehenbuhl, Jean Pierre. Universite de Lausanne; Suiza
Fil: Sirard, Jean Claude. Universite de Lausanne; Suiza
Materia
HIV VACCINE
HLA-A2
RECOMBINANT POXVIRUSES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/162854
Acceder
id CONICETDig_ea2ebc2d4c342a4555e1f392fc007a92
oai_identifier_str oai:ri.conicet.gov.ar:11336/162854
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Attenuated poxviruses expressing a synthetic HIV protein stimulate HLA-A2-restricted cytotoxic T-cell responsesDidierlaurent, ArnaudRamirez, Juan-CarlosGherardi, Maria MagdalenaZimmerli, Simone C.Graf, MarcusOrbea, Hans AchaPantaleo, GiuseppeWagner, RalfEsteban, MarianoKraehenbuhl, Jean PierreSirard, Jean ClaudeHIV VACCINEHLA-A2RECOMBINANT POXVIRUSEShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Efficient HIV vaccines have to trigger cell-mediated immunity directed against various viral antigens. However little is known about the breadth of the response induced by vaccines carrying multiple proteins. Here, we report on the immunogenicity of a construct harbouring a fusion of the HIV-1 IIIB gag, pol and nef genes (gpn) designed for optimal safety and equimolar expression of the HIV proteins. The attenuated poxviruses, MVA and NYVAC, harbouring the gpn construct, induced potent immune responses in conventional mice characterised by stimulation of Gpn-specific IFN-γ-producing cells and cytotoxic T cells. In HLA-A2 transgenic mice, recombinant MVA elicited cytotoxic responses against epitopes recognised in most HLA-A2+ HIV-1-infected individuals. We also found that the MVA vaccine triggered the in vitro expansion of peripheral blood cells isolated from a HIV-1-seropositive patient and with similar specificity as found in immunised HLA-A2 transgenic mice. In conclusion, the synthetic HIV polyantigen Gpn delivered by MVA is immunogenic, efficiently processed and presented by human MHC class I molecules.Fil: Didierlaurent, Arnaud. Universite de Lausanne; SuizaFil: Ramirez, Juan-Carlos. Consejo Superior de Investigaciones Científicas; EspañaFil: Gherardi, Maria Magdalena. Consejo Superior de Investigaciones Científicas; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zimmerli, Simone C.. No especifíca;Fil: Graf, Marcus. No especifíca;Fil: Orbea, Hans Acha. Universite de Lausanne; SuizaFil: Pantaleo, Giuseppe. No especifíca;Fil: Wagner, Ralf. No especifíca;Fil: Esteban, Mariano. Consejo Superior de Investigaciones Científicas; EspañaFil: Kraehenbuhl, Jean Pierre. Universite de Lausanne; SuizaFil: Sirard, Jean Claude. Universite de Lausanne; SuizaElsevier2004-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/162854Didierlaurent, Arnaud; Ramirez, Juan-Carlos; Gherardi, Maria Magdalena; Zimmerli, Simone C.; Graf, Marcus; et al.; Attenuated poxviruses expressing a synthetic HIV protein stimulate HLA-A2-restricted cytotoxic T-cell responses; Elsevier; Vaccine; 22; 25-26; 9-2004; 3395-34030264-410XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X04001872info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2004.02.025info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:16Zoai:ri.conicet.gov.ar:11336/162854instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:16.85CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Attenuated poxviruses expressing a synthetic HIV protein stimulate HLA-A2-restricted cytotoxic T-cell responses
title Attenuated poxviruses expressing a synthetic HIV protein stimulate HLA-A2-restricted cytotoxic T-cell responses
spellingShingle Attenuated poxviruses expressing a synthetic HIV protein stimulate HLA-A2-restricted cytotoxic T-cell responses
Didierlaurent, Arnaud
HIV VACCINE
HLA-A2
RECOMBINANT POXVIRUSES
title_short Attenuated poxviruses expressing a synthetic HIV protein stimulate HLA-A2-restricted cytotoxic T-cell responses
title_full Attenuated poxviruses expressing a synthetic HIV protein stimulate HLA-A2-restricted cytotoxic T-cell responses
title_fullStr Attenuated poxviruses expressing a synthetic HIV protein stimulate HLA-A2-restricted cytotoxic T-cell responses
title_full_unstemmed Attenuated poxviruses expressing a synthetic HIV protein stimulate HLA-A2-restricted cytotoxic T-cell responses
title_sort Attenuated poxviruses expressing a synthetic HIV protein stimulate HLA-A2-restricted cytotoxic T-cell responses
dc.creator.none.fl_str_mv Didierlaurent, Arnaud
Ramirez, Juan-Carlos
Gherardi, Maria Magdalena
Zimmerli, Simone C.
Graf, Marcus
Orbea, Hans Acha
Pantaleo, Giuseppe
Wagner, Ralf
Esteban, Mariano
Kraehenbuhl, Jean Pierre
Sirard, Jean Claude
author Didierlaurent, Arnaud
author_facet Didierlaurent, Arnaud
Ramirez, Juan-Carlos
Gherardi, Maria Magdalena
Zimmerli, Simone C.
Graf, Marcus
Orbea, Hans Acha
Pantaleo, Giuseppe
Wagner, Ralf
Esteban, Mariano
Kraehenbuhl, Jean Pierre
Sirard, Jean Claude
author_role author
author2 Ramirez, Juan-Carlos
Gherardi, Maria Magdalena
Zimmerli, Simone C.
Graf, Marcus
Orbea, Hans Acha
Pantaleo, Giuseppe
Wagner, Ralf
Esteban, Mariano
Kraehenbuhl, Jean Pierre
Sirard, Jean Claude
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv HIV VACCINE
HLA-A2
RECOMBINANT POXVIRUSES
topic HIV VACCINE
HLA-A2
RECOMBINANT POXVIRUSES
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Efficient HIV vaccines have to trigger cell-mediated immunity directed against various viral antigens. However little is known about the breadth of the response induced by vaccines carrying multiple proteins. Here, we report on the immunogenicity of a construct harbouring a fusion of the HIV-1 IIIB gag, pol and nef genes (gpn) designed for optimal safety and equimolar expression of the HIV proteins. The attenuated poxviruses, MVA and NYVAC, harbouring the gpn construct, induced potent immune responses in conventional mice characterised by stimulation of Gpn-specific IFN-γ-producing cells and cytotoxic T cells. In HLA-A2 transgenic mice, recombinant MVA elicited cytotoxic responses against epitopes recognised in most HLA-A2+ HIV-1-infected individuals. We also found that the MVA vaccine triggered the in vitro expansion of peripheral blood cells isolated from a HIV-1-seropositive patient and with similar specificity as found in immunised HLA-A2 transgenic mice. In conclusion, the synthetic HIV polyantigen Gpn delivered by MVA is immunogenic, efficiently processed and presented by human MHC class I molecules.
Fil: Didierlaurent, Arnaud. Universite de Lausanne; Suiza
Fil: Ramirez, Juan-Carlos. Consejo Superior de Investigaciones Científicas; España
Fil: Gherardi, Maria Magdalena. Consejo Superior de Investigaciones Científicas; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zimmerli, Simone C.. No especifíca;
Fil: Graf, Marcus. No especifíca;
Fil: Orbea, Hans Acha. Universite de Lausanne; Suiza
Fil: Pantaleo, Giuseppe. No especifíca;
Fil: Wagner, Ralf. No especifíca;
Fil: Esteban, Mariano. Consejo Superior de Investigaciones Científicas; España
Fil: Kraehenbuhl, Jean Pierre. Universite de Lausanne; Suiza
Fil: Sirard, Jean Claude. Universite de Lausanne; Suiza
description Efficient HIV vaccines have to trigger cell-mediated immunity directed against various viral antigens. However little is known about the breadth of the response induced by vaccines carrying multiple proteins. Here, we report on the immunogenicity of a construct harbouring a fusion of the HIV-1 IIIB gag, pol and nef genes (gpn) designed for optimal safety and equimolar expression of the HIV proteins. The attenuated poxviruses, MVA and NYVAC, harbouring the gpn construct, induced potent immune responses in conventional mice characterised by stimulation of Gpn-specific IFN-γ-producing cells and cytotoxic T cells. In HLA-A2 transgenic mice, recombinant MVA elicited cytotoxic responses against epitopes recognised in most HLA-A2+ HIV-1-infected individuals. We also found that the MVA vaccine triggered the in vitro expansion of peripheral blood cells isolated from a HIV-1-seropositive patient and with similar specificity as found in immunised HLA-A2 transgenic mice. In conclusion, the synthetic HIV polyantigen Gpn delivered by MVA is immunogenic, efficiently processed and presented by human MHC class I molecules.
publishDate 2004
dc.date.none.fl_str_mv 2004-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/162854
Didierlaurent, Arnaud; Ramirez, Juan-Carlos; Gherardi, Maria Magdalena; Zimmerli, Simone C.; Graf, Marcus; et al.; Attenuated poxviruses expressing a synthetic HIV protein stimulate HLA-A2-restricted cytotoxic T-cell responses; Elsevier; Vaccine; 22; 25-26; 9-2004; 3395-3403
0264-410X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/162854
identifier_str_mv Didierlaurent, Arnaud; Ramirez, Juan-Carlos; Gherardi, Maria Magdalena; Zimmerli, Simone C.; Graf, Marcus; et al.; Attenuated poxviruses expressing a synthetic HIV protein stimulate HLA-A2-restricted cytotoxic T-cell responses; Elsevier; Vaccine; 22; 25-26; 9-2004; 3395-3403
0264-410X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X04001872
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2004.02.025
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.260194

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