Identification, molecular and phylogenetic analysis of poxvirus in skin lesions of southern right whale

Autores
Fiorito, Carla Daniela; Palacios, Carlos Adolfo; Golemba, Marcelo Darío; Bratanich, Ana Cristina; Argüelles, María Belén; Fazio, Ana; Bertellotti, Néstor Marcelo; Lombardo, Daniel Marcelo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Poxvirus skin disease has been reported in several species of cetaceans, principally in odontocetes, and a single report in mysticetes. Southern right whales Eubalaena australis in Peninsula Valdes, Argentina, show a variety of skin lesions of unknown etiology, and the number of these lesions has increased in recent years. Samples from dead whales were taken in order to establish the etiology of these lesions. One calf and one adult presented ring-type lesions, characterized by a circumscribed and slightly raised area of skin. Lesions were histologically characterized by the presence of microvesicles and vacuolated cells in the stratum spinosum, along with hyperplasia of the stratum corneum and eosinophilic inclusion bodies in the cytoplasm of the epithelial cells. Transmission electron microscopy showed aggregations of virions with typical poxvirus morphology. PCR of cetacean poxvirus (CPV) DNA polymerase, DNA topoisomerase I and parapoxvirus DNA polymerase gene fragments was done, and confirmed the presence of poxvirus in one sample. Phylogenetic analysis showed that the detected poxvirus belongs to the CPV-2 group. This is the first confirmed report of poxvirus in southern right whales in Argentina.
Fil: Fiorito, Carla Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Palacios, Carlos Adolfo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología ; Argentina
Fil: Golemba, Marcelo Darío. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría ; Argentina
Fil: Bratanich, Ana Cristina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Argüelles, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Fazio, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Bertellotti, Néstor Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Lombardo, Daniel Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Materia
Cetacean Poxvirus
Skin Disease
Southern Right Whale
Eubalaena Australis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/44396
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/44396
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Identification, molecular and phylogenetic analysis of poxvirus in skin lesions of southern right whaleFiorito, Carla DanielaPalacios, Carlos AdolfoGolemba, Marcelo DaríoBratanich, Ana CristinaArgüelles, María BelénFazio, AnaBertellotti, Néstor MarceloLombardo, Daniel MarceloCetacean PoxvirusSkin DiseaseSouthern Right WhaleEubalaena Australishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Poxvirus skin disease has been reported in several species of cetaceans, principally in odontocetes, and a single report in mysticetes. Southern right whales Eubalaena australis in Peninsula Valdes, Argentina, show a variety of skin lesions of unknown etiology, and the number of these lesions has increased in recent years. Samples from dead whales were taken in order to establish the etiology of these lesions. One calf and one adult presented ring-type lesions, characterized by a circumscribed and slightly raised area of skin. Lesions were histologically characterized by the presence of microvesicles and vacuolated cells in the stratum spinosum, along with hyperplasia of the stratum corneum and eosinophilic inclusion bodies in the cytoplasm of the epithelial cells. Transmission electron microscopy showed aggregations of virions with typical poxvirus morphology. PCR of cetacean poxvirus (CPV) DNA polymerase, DNA topoisomerase I and parapoxvirus DNA polymerase gene fragments was done, and confirmed the presence of poxvirus in one sample. Phylogenetic analysis showed that the detected poxvirus belongs to the CPV-2 group. This is the first confirmed report of poxvirus in southern right whales in Argentina.Fil: Fiorito, Carla Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Palacios, Carlos Adolfo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología ; ArgentinaFil: Golemba, Marcelo Darío. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría ; ArgentinaFil: Bratanich, Ana Cristina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaFil: Argüelles, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Fazio, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Bertellotti, Néstor Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Lombardo, Daniel Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaInter-Research2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/44396Fiorito, Carla Daniela; Palacios, Carlos Adolfo; Golemba, Marcelo Darío; Bratanich, Ana Cristina; Argüelles, María Belén; et al.; Identification, molecular and phylogenetic analysis of poxvirus in skin lesions of southern right whale; Inter-Research; Diseases Of Aquatic Organisms; 116; 2; 10-2015; 157-1630177-51031616-1580CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.int-res.com/abstracts/dao/v116/n2/p157-163/info:eu-repo/semantics/altIdentifier/doi/10.3354/dao02918info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:36:54Zoai:ri.conicet.gov.ar:11336/44396instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:36:54.293CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification, molecular and phylogenetic analysis of poxvirus in skin lesions of southern right whale
title Identification, molecular and phylogenetic analysis of poxvirus in skin lesions of southern right whale
spellingShingle Identification, molecular and phylogenetic analysis of poxvirus in skin lesions of southern right whale
Fiorito, Carla Daniela
Cetacean Poxvirus
Skin Disease
Southern Right Whale
Eubalaena Australis
title_short Identification, molecular and phylogenetic analysis of poxvirus in skin lesions of southern right whale
title_full Identification, molecular and phylogenetic analysis of poxvirus in skin lesions of southern right whale
title_fullStr Identification, molecular and phylogenetic analysis of poxvirus in skin lesions of southern right whale
title_full_unstemmed Identification, molecular and phylogenetic analysis of poxvirus in skin lesions of southern right whale
title_sort Identification, molecular and phylogenetic analysis of poxvirus in skin lesions of southern right whale
dc.creator.none.fl_str_mv Fiorito, Carla Daniela
Palacios, Carlos Adolfo
Golemba, Marcelo Darío
Bratanich, Ana Cristina
Argüelles, María Belén
Fazio, Ana
Bertellotti, Néstor Marcelo
Lombardo, Daniel Marcelo
author Fiorito, Carla Daniela
author_facet Fiorito, Carla Daniela
Palacios, Carlos Adolfo
Golemba, Marcelo Darío
Bratanich, Ana Cristina
Argüelles, María Belén
Fazio, Ana
Bertellotti, Néstor Marcelo
Lombardo, Daniel Marcelo
author_role author
author2 Palacios, Carlos Adolfo
Golemba, Marcelo Darío
Bratanich, Ana Cristina
Argüelles, María Belén
Fazio, Ana
Bertellotti, Néstor Marcelo
Lombardo, Daniel Marcelo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cetacean Poxvirus
Skin Disease
Southern Right Whale
Eubalaena Australis
topic Cetacean Poxvirus
Skin Disease
Southern Right Whale
Eubalaena Australis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Poxvirus skin disease has been reported in several species of cetaceans, principally in odontocetes, and a single report in mysticetes. Southern right whales Eubalaena australis in Peninsula Valdes, Argentina, show a variety of skin lesions of unknown etiology, and the number of these lesions has increased in recent years. Samples from dead whales were taken in order to establish the etiology of these lesions. One calf and one adult presented ring-type lesions, characterized by a circumscribed and slightly raised area of skin. Lesions were histologically characterized by the presence of microvesicles and vacuolated cells in the stratum spinosum, along with hyperplasia of the stratum corneum and eosinophilic inclusion bodies in the cytoplasm of the epithelial cells. Transmission electron microscopy showed aggregations of virions with typical poxvirus morphology. PCR of cetacean poxvirus (CPV) DNA polymerase, DNA topoisomerase I and parapoxvirus DNA polymerase gene fragments was done, and confirmed the presence of poxvirus in one sample. Phylogenetic analysis showed that the detected poxvirus belongs to the CPV-2 group. This is the first confirmed report of poxvirus in southern right whales in Argentina.
Fil: Fiorito, Carla Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Palacios, Carlos Adolfo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología ; Argentina
Fil: Golemba, Marcelo Darío. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría ; Argentina
Fil: Bratanich, Ana Cristina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Argüelles, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Fazio, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Bertellotti, Néstor Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Lombardo, Daniel Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
description Poxvirus skin disease has been reported in several species of cetaceans, principally in odontocetes, and a single report in mysticetes. Southern right whales Eubalaena australis in Peninsula Valdes, Argentina, show a variety of skin lesions of unknown etiology, and the number of these lesions has increased in recent years. Samples from dead whales were taken in order to establish the etiology of these lesions. One calf and one adult presented ring-type lesions, characterized by a circumscribed and slightly raised area of skin. Lesions were histologically characterized by the presence of microvesicles and vacuolated cells in the stratum spinosum, along with hyperplasia of the stratum corneum and eosinophilic inclusion bodies in the cytoplasm of the epithelial cells. Transmission electron microscopy showed aggregations of virions with typical poxvirus morphology. PCR of cetacean poxvirus (CPV) DNA polymerase, DNA topoisomerase I and parapoxvirus DNA polymerase gene fragments was done, and confirmed the presence of poxvirus in one sample. Phylogenetic analysis showed that the detected poxvirus belongs to the CPV-2 group. This is the first confirmed report of poxvirus in southern right whales in Argentina.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/44396
Fiorito, Carla Daniela; Palacios, Carlos Adolfo; Golemba, Marcelo Darío; Bratanich, Ana Cristina; Argüelles, María Belén; et al.; Identification, molecular and phylogenetic analysis of poxvirus in skin lesions of southern right whale; Inter-Research; Diseases Of Aquatic Organisms; 116; 2; 10-2015; 157-163
0177-5103
1616-1580
CONICET Digital
CONICET
url http://hdl.handle.net/11336/44396
identifier_str_mv Fiorito, Carla Daniela; Palacios, Carlos Adolfo; Golemba, Marcelo Darío; Bratanich, Ana Cristina; Argüelles, María Belén; et al.; Identification, molecular and phylogenetic analysis of poxvirus in skin lesions of southern right whale; Inter-Research; Diseases Of Aquatic Organisms; 116; 2; 10-2015; 157-163
0177-5103
1616-1580
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.3354/dao02918
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Inter-Research
publisher.none.fl_str_mv Inter-Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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