Towards a better method of cannabinoid extraction and preservation for cannabis-based products

Autores
Chamorro Aguirre, Estefania; Pascual, Ana Clara; Milano, Pablo Gustavo; Bocetti, Marisol Claudia; Pasquaré, Susana Juana
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Recombinant human erythropoietin (Epo) has been successful in the treatment of anemia of different etiologies but high Epo doses, used to reduce the frequency of administration or to treat refractory anemia, have been linked with adverse outcomes. Previously, we observed a decreased effect of high Epo concentrations on endothelial cell migration. Here, we studied the antiapoptotic effect of Epo on erythroleukemia K562 cells exposed to TNF-a (T), after incubation with increasing concentrations of Epo (10, 100 and 400 U/mL) and erythroid differentiation with hemin. While Epo10 prevented the increase in the percentage of apoptotic nuclei observed with TNF-a, Epo100 and Epo400 did not show that protection. Considering that this effect could be related to the decreased availability of the Epo receptor, we investigated the activity of protein tyrosine phosphatase 1B (PTP1B) and the process of proteasomal degradation in this cell model. PTP1B activity, which regulates many aspects of the signaling pathways elicited by Epo upon EpoR activation, showed no significant differences between cultures with low and high Epo concentrations. Then, we used the proteasome inhibitor MG132 to evaluate if EpoR internalization could affect the antiapoptotic effect of Epo against TNF-a. In the presence of MG132, the results with high concentrations of Epo were similar to those observed with Epo10T (Hoechst staining, C 13.5 ± 0.7, T 31.5 ± 1.5*, Epo10T 22.1 ± 1.1*, Epo100T 29.4 ± 1.4*, Epo400T 29.9 ± 2.2*, Epo100TMG 21.7 ± 19, and Epo400TMG 22 ± 1.3 %, *P<0.05, n= 6). Based on this, it can be suggested that the kinetics of EpoR internalization/degradation, mediated by the proteasome, could explain, at least in part, the lack of antiapoptotic effect of high concentrations of Epo. The present results give new insights that may contribute to understand Epo behavior when cells of different tissues are activated by a high concentration of the hormone, and may help to adequate therapeutic strategies.
Fil: Chamorro Aguirre, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pascual, Ana Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Milano, Pablo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Bocetti, Marisol Claudia. Provincia de Buenos Aires. Municipalidad de Bahía Blanca; Argentina
Fil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Reunión Anual de Sociedades de Biociencia
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Asociación Argentina de Nanomedicinas
Materia
DELTA-9-TETRAHYDROCANNABINOL
CANNABIDIOL
EXTRACTION
QUANTIFICATION
STORAGE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/156335
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Towards a better method of cannabinoid extraction and preservation for cannabis-based productsChamorro Aguirre, EstefaniaPascual, Ana ClaraMilano, Pablo GustavoBocetti, Marisol ClaudiaPasquaré, Susana JuanaDELTA-9-TETRAHYDROCANNABINOLCANNABIDIOLEXTRACTIONQUANTIFICATIONSTORAGEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Recombinant human erythropoietin (Epo) has been successful in the treatment of anemia of different etiologies but high Epo doses, used to reduce the frequency of administration or to treat refractory anemia, have been linked with adverse outcomes. Previously, we observed a decreased effect of high Epo concentrations on endothelial cell migration. Here, we studied the antiapoptotic effect of Epo on erythroleukemia K562 cells exposed to TNF-a (T), after incubation with increasing concentrations of Epo (10, 100 and 400 U/mL) and erythroid differentiation with hemin. While Epo10 prevented the increase in the percentage of apoptotic nuclei observed with TNF-a, Epo100 and Epo400 did not show that protection. Considering that this effect could be related to the decreased availability of the Epo receptor, we investigated the activity of protein tyrosine phosphatase 1B (PTP1B) and the process of proteasomal degradation in this cell model. PTP1B activity, which regulates many aspects of the signaling pathways elicited by Epo upon EpoR activation, showed no significant differences between cultures with low and high Epo concentrations. Then, we used the proteasome inhibitor MG132 to evaluate if EpoR internalization could affect the antiapoptotic effect of Epo against TNF-a. In the presence of MG132, the results with high concentrations of Epo were similar to those observed with Epo10T (Hoechst staining, C 13.5 ± 0.7, T 31.5 ± 1.5*, Epo10T 22.1 ± 1.1*, Epo100T 29.4 ± 1.4*, Epo400T 29.9 ± 2.2*, Epo100TMG 21.7 ± 19, and Epo400TMG 22 ± 1.3 %, *P<0.05, n= 6). Based on this, it can be suggested that the kinetics of EpoR internalization/degradation, mediated by the proteasome, could explain, at least in part, the lack of antiapoptotic effect of high concentrations of Epo. The present results give new insights that may contribute to understand Epo behavior when cells of different tissues are activated by a high concentration of the hormone, and may help to adequate therapeutic strategies.Fil: Chamorro Aguirre, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Pascual, Ana Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Milano, Pablo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Bocetti, Marisol Claudia. Provincia de Buenos Aires. Municipalidad de Bahía Blanca; ArgentinaFil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaReunión Anual de Sociedades de BiocienciaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalSociedad Argentina de BiologíaAsociación Argentina de NanomedicinasFundación Revista Medicina2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/156335Towards a better method of cannabinoid extraction and preservation for cannabis-based products; Reunión Anual de Sociedades de Biociencia; Mar del Plata; Argentina; 2019; 163-1630025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicinaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:06Zoai:ri.conicet.gov.ar:11336/156335instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:06.481CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Towards a better method of cannabinoid extraction and preservation for cannabis-based products
title Towards a better method of cannabinoid extraction and preservation for cannabis-based products
spellingShingle Towards a better method of cannabinoid extraction and preservation for cannabis-based products
Chamorro Aguirre, Estefania
DELTA-9-TETRAHYDROCANNABINOL
CANNABIDIOL
EXTRACTION
QUANTIFICATION
STORAGE
title_short Towards a better method of cannabinoid extraction and preservation for cannabis-based products
title_full Towards a better method of cannabinoid extraction and preservation for cannabis-based products
title_fullStr Towards a better method of cannabinoid extraction and preservation for cannabis-based products
title_full_unstemmed Towards a better method of cannabinoid extraction and preservation for cannabis-based products
title_sort Towards a better method of cannabinoid extraction and preservation for cannabis-based products
dc.creator.none.fl_str_mv Chamorro Aguirre, Estefania
Pascual, Ana Clara
Milano, Pablo Gustavo
Bocetti, Marisol Claudia
Pasquaré, Susana Juana
author Chamorro Aguirre, Estefania
author_facet Chamorro Aguirre, Estefania
Pascual, Ana Clara
Milano, Pablo Gustavo
Bocetti, Marisol Claudia
Pasquaré, Susana Juana
author_role author
author2 Pascual, Ana Clara
Milano, Pablo Gustavo
Bocetti, Marisol Claudia
Pasquaré, Susana Juana
author2_role author
author
author
author
dc.subject.none.fl_str_mv DELTA-9-TETRAHYDROCANNABINOL
CANNABIDIOL
EXTRACTION
QUANTIFICATION
STORAGE
topic DELTA-9-TETRAHYDROCANNABINOL
CANNABIDIOL
EXTRACTION
QUANTIFICATION
STORAGE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Recombinant human erythropoietin (Epo) has been successful in the treatment of anemia of different etiologies but high Epo doses, used to reduce the frequency of administration or to treat refractory anemia, have been linked with adverse outcomes. Previously, we observed a decreased effect of high Epo concentrations on endothelial cell migration. Here, we studied the antiapoptotic effect of Epo on erythroleukemia K562 cells exposed to TNF-a (T), after incubation with increasing concentrations of Epo (10, 100 and 400 U/mL) and erythroid differentiation with hemin. While Epo10 prevented the increase in the percentage of apoptotic nuclei observed with TNF-a, Epo100 and Epo400 did not show that protection. Considering that this effect could be related to the decreased availability of the Epo receptor, we investigated the activity of protein tyrosine phosphatase 1B (PTP1B) and the process of proteasomal degradation in this cell model. PTP1B activity, which regulates many aspects of the signaling pathways elicited by Epo upon EpoR activation, showed no significant differences between cultures with low and high Epo concentrations. Then, we used the proteasome inhibitor MG132 to evaluate if EpoR internalization could affect the antiapoptotic effect of Epo against TNF-a. In the presence of MG132, the results with high concentrations of Epo were similar to those observed with Epo10T (Hoechst staining, C 13.5 ± 0.7, T 31.5 ± 1.5*, Epo10T 22.1 ± 1.1*, Epo100T 29.4 ± 1.4*, Epo400T 29.9 ± 2.2*, Epo100TMG 21.7 ± 19, and Epo400TMG 22 ± 1.3 %, *P<0.05, n= 6). Based on this, it can be suggested that the kinetics of EpoR internalization/degradation, mediated by the proteasome, could explain, at least in part, the lack of antiapoptotic effect of high concentrations of Epo. The present results give new insights that may contribute to understand Epo behavior when cells of different tissues are activated by a high concentration of the hormone, and may help to adequate therapeutic strategies.
Fil: Chamorro Aguirre, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pascual, Ana Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Milano, Pablo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Bocetti, Marisol Claudia. Provincia de Buenos Aires. Municipalidad de Bahía Blanca; Argentina
Fil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Reunión Anual de Sociedades de Biociencia
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Asociación Argentina de Nanomedicinas
description Recombinant human erythropoietin (Epo) has been successful in the treatment of anemia of different etiologies but high Epo doses, used to reduce the frequency of administration or to treat refractory anemia, have been linked with adverse outcomes. Previously, we observed a decreased effect of high Epo concentrations on endothelial cell migration. Here, we studied the antiapoptotic effect of Epo on erythroleukemia K562 cells exposed to TNF-a (T), after incubation with increasing concentrations of Epo (10, 100 and 400 U/mL) and erythroid differentiation with hemin. While Epo10 prevented the increase in the percentage of apoptotic nuclei observed with TNF-a, Epo100 and Epo400 did not show that protection. Considering that this effect could be related to the decreased availability of the Epo receptor, we investigated the activity of protein tyrosine phosphatase 1B (PTP1B) and the process of proteasomal degradation in this cell model. PTP1B activity, which regulates many aspects of the signaling pathways elicited by Epo upon EpoR activation, showed no significant differences between cultures with low and high Epo concentrations. Then, we used the proteasome inhibitor MG132 to evaluate if EpoR internalization could affect the antiapoptotic effect of Epo against TNF-a. In the presence of MG132, the results with high concentrations of Epo were similar to those observed with Epo10T (Hoechst staining, C 13.5 ± 0.7, T 31.5 ± 1.5*, Epo10T 22.1 ± 1.1*, Epo100T 29.4 ± 1.4*, Epo400T 29.9 ± 2.2*, Epo100TMG 21.7 ± 19, and Epo400TMG 22 ± 1.3 %, *P<0.05, n= 6). Based on this, it can be suggested that the kinetics of EpoR internalization/degradation, mediated by the proteasome, could explain, at least in part, the lack of antiapoptotic effect of high concentrations of Epo. The present results give new insights that may contribute to understand Epo behavior when cells of different tissues are activated by a high concentration of the hormone, and may help to adequate therapeutic strategies.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/156335
Towards a better method of cannabinoid extraction and preservation for cannabis-based products; Reunión Anual de Sociedades de Biociencia; Mar del Plata; Argentina; 2019; 163-163
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/156335
identifier_str_mv Towards a better method of cannabinoid extraction and preservation for cannabis-based products; Reunión Anual de Sociedades de Biociencia; Mar del Plata; Argentina; 2019; 163-163
0025-7680
1669-9106
CONICET Digital
CONICET
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language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicina
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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