Alterations in innate and adaptive immune leukocytes are involved in paediatric obesity

Autores
Inzaugarat, Maria Eugenia; Billordo, Luis Ariel; Vodánovich, F.; Cervini Bohm, Gabriela Marta ; Casavalle, Patricia Lucía; Vedire, C.; Cherñavsky, Alejandra Claudia
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Adipose tissue is the main source of the cytokines and adipokines that are increased in the context of obesity. The production of reactive oxygen species (ROS) and cytokines by circulating immune cells can be regulated by these pro-inflammatory factors even before infiltration into adipose tissue. Objective: To investigate the alterations that can occur in circulating monocytes and lymphocytes in paediatric obesity. Methods: In this study, 54 paediatric obese patients and 30 age-matched metabolically healthy individuals were enrolled. Intracellular cytokines were analyzed after phorbol myristate acetate (PMA) or leptin plus PMA stimulation of lymphocytes and monocytes by flow cytometry. ROS generation was measured using dichlorofluorescein-diacetate. Both a 'stimulation index' and a 'fold of increase' were calculated for statistical purposes. Results: Both interferon gamma (IFN-γ) production by circulating CD4+ and CD8+ lymphocytes and ROS production by monocytes following PMA stimulation were increased in obese patients. Leptin induced an increased production of IFN-γ in both subsets of T cells and tumour necrosis factor alpha in monocytes, and linoleic acid induced a higher ROS production in monocytes. Conclusions: The distinct functional responses of circulating cells suggest that alterations in both innate and adaptive immune cells are involved in the maintenance of low-grade inflammation in paediatric obesity.
Fil: Inzaugarat, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Billordo, Luis Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Vodánovich, F.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Cervini Bohm, Gabriela Marta . Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Casavalle, Patricia Lucía. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Vedire, C.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Cherñavsky, Alejandra Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo; Argentina
Materia
MONOCYTES
OXIDATIVE STRESS
T CELLS
TH1/TH2 CYTOKINES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8522
Acceder
id CONICETDig_e9c5a7d0c7e9fbced5be280d4852cb8c
oai_identifier_str oai:ri.conicet.gov.ar:11336/8522
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Alterations in innate and adaptive immune leukocytes are involved in paediatric obesityInzaugarat, Maria EugeniaBillordo, Luis ArielVodánovich, F.Cervini Bohm, Gabriela Marta Casavalle, Patricia LucíaVedire, C.Cherñavsky, Alejandra ClaudiaMONOCYTESOXIDATIVE STRESST CELLSTH1/TH2 CYTOKINEShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background: Adipose tissue is the main source of the cytokines and adipokines that are increased in the context of obesity. The production of reactive oxygen species (ROS) and cytokines by circulating immune cells can be regulated by these pro-inflammatory factors even before infiltration into adipose tissue. Objective: To investigate the alterations that can occur in circulating monocytes and lymphocytes in paediatric obesity. Methods: In this study, 54 paediatric obese patients and 30 age-matched metabolically healthy individuals were enrolled. Intracellular cytokines were analyzed after phorbol myristate acetate (PMA) or leptin plus PMA stimulation of lymphocytes and monocytes by flow cytometry. ROS generation was measured using dichlorofluorescein-diacetate. Both a 'stimulation index' and a 'fold of increase' were calculated for statistical purposes. Results: Both interferon gamma (IFN-γ) production by circulating CD4+ and CD8+ lymphocytes and ROS production by monocytes following PMA stimulation were increased in obese patients. Leptin induced an increased production of IFN-γ in both subsets of T cells and tumour necrosis factor alpha in monocytes, and linoleic acid induced a higher ROS production in monocytes. Conclusions: The distinct functional responses of circulating cells suggest that alterations in both innate and adaptive immune cells are involved in the maintenance of low-grade inflammation in paediatric obesity.Fil: Inzaugarat, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Billordo, Luis Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Vodánovich, F.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Cervini Bohm, Gabriela Marta . Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Casavalle, Patricia Lucía. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Vedire, C.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Cherñavsky, Alejandra Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo; ArgentinaWiley2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8522Inzaugarat, Maria Eugenia; Billordo, Luis Ariel; Vodánovich, F.; Cervini Bohm, Gabriela Marta ; Casavalle, Patricia Lucía; et al.; Alterations in innate and adaptive immune leukocytes are involved in paediatric obesity; Wiley; Pediatric Obesity; 9; 5; 7-2013; 381-3902047-6310enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.2047-6310.2013.00179.x/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.2047-6310.2013.00179.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:39Zoai:ri.conicet.gov.ar:11336/8522instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:40.09CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Alterations in innate and adaptive immune leukocytes are involved in paediatric obesity
title Alterations in innate and adaptive immune leukocytes are involved in paediatric obesity
spellingShingle Alterations in innate and adaptive immune leukocytes are involved in paediatric obesity
Inzaugarat, Maria Eugenia
MONOCYTES
OXIDATIVE STRESS
T CELLS
TH1/TH2 CYTOKINES
title_short Alterations in innate and adaptive immune leukocytes are involved in paediatric obesity
title_full Alterations in innate and adaptive immune leukocytes are involved in paediatric obesity
title_fullStr Alterations in innate and adaptive immune leukocytes are involved in paediatric obesity
title_full_unstemmed Alterations in innate and adaptive immune leukocytes are involved in paediatric obesity
title_sort Alterations in innate and adaptive immune leukocytes are involved in paediatric obesity
dc.creator.none.fl_str_mv Inzaugarat, Maria Eugenia
Billordo, Luis Ariel
Vodánovich, F.
Cervini Bohm, Gabriela Marta 
Casavalle, Patricia Lucía
Vedire, C.
Cherñavsky, Alejandra Claudia
author Inzaugarat, Maria Eugenia
author_facet Inzaugarat, Maria Eugenia
Billordo, Luis Ariel
Vodánovich, F.
Cervini Bohm, Gabriela Marta 
Casavalle, Patricia Lucía
Vedire, C.
Cherñavsky, Alejandra Claudia
author_role author
author2 Billordo, Luis Ariel
Vodánovich, F.
Cervini Bohm, Gabriela Marta 
Casavalle, Patricia Lucía
Vedire, C.
Cherñavsky, Alejandra Claudia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv MONOCYTES
OXIDATIVE STRESS
T CELLS
TH1/TH2 CYTOKINES
topic MONOCYTES
OXIDATIVE STRESS
T CELLS
TH1/TH2 CYTOKINES
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Adipose tissue is the main source of the cytokines and adipokines that are increased in the context of obesity. The production of reactive oxygen species (ROS) and cytokines by circulating immune cells can be regulated by these pro-inflammatory factors even before infiltration into adipose tissue. Objective: To investigate the alterations that can occur in circulating monocytes and lymphocytes in paediatric obesity. Methods: In this study, 54 paediatric obese patients and 30 age-matched metabolically healthy individuals were enrolled. Intracellular cytokines were analyzed after phorbol myristate acetate (PMA) or leptin plus PMA stimulation of lymphocytes and monocytes by flow cytometry. ROS generation was measured using dichlorofluorescein-diacetate. Both a 'stimulation index' and a 'fold of increase' were calculated for statistical purposes. Results: Both interferon gamma (IFN-γ) production by circulating CD4+ and CD8+ lymphocytes and ROS production by monocytes following PMA stimulation were increased in obese patients. Leptin induced an increased production of IFN-γ in both subsets of T cells and tumour necrosis factor alpha in monocytes, and linoleic acid induced a higher ROS production in monocytes. Conclusions: The distinct functional responses of circulating cells suggest that alterations in both innate and adaptive immune cells are involved in the maintenance of low-grade inflammation in paediatric obesity.
Fil: Inzaugarat, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Billordo, Luis Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Vodánovich, F.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Cervini Bohm, Gabriela Marta . Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Casavalle, Patricia Lucía. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Vedire, C.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Cherñavsky, Alejandra Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo; Argentina
description Background: Adipose tissue is the main source of the cytokines and adipokines that are increased in the context of obesity. The production of reactive oxygen species (ROS) and cytokines by circulating immune cells can be regulated by these pro-inflammatory factors even before infiltration into adipose tissue. Objective: To investigate the alterations that can occur in circulating monocytes and lymphocytes in paediatric obesity. Methods: In this study, 54 paediatric obese patients and 30 age-matched metabolically healthy individuals were enrolled. Intracellular cytokines were analyzed after phorbol myristate acetate (PMA) or leptin plus PMA stimulation of lymphocytes and monocytes by flow cytometry. ROS generation was measured using dichlorofluorescein-diacetate. Both a 'stimulation index' and a 'fold of increase' were calculated for statistical purposes. Results: Both interferon gamma (IFN-γ) production by circulating CD4+ and CD8+ lymphocytes and ROS production by monocytes following PMA stimulation were increased in obese patients. Leptin induced an increased production of IFN-γ in both subsets of T cells and tumour necrosis factor alpha in monocytes, and linoleic acid induced a higher ROS production in monocytes. Conclusions: The distinct functional responses of circulating cells suggest that alterations in both innate and adaptive immune cells are involved in the maintenance of low-grade inflammation in paediatric obesity.
publishDate 2013
dc.date.none.fl_str_mv 2013-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8522
Inzaugarat, Maria Eugenia; Billordo, Luis Ariel; Vodánovich, F.; Cervini Bohm, Gabriela Marta ; Casavalle, Patricia Lucía; et al.; Alterations in innate and adaptive immune leukocytes are involved in paediatric obesity; Wiley; Pediatric Obesity; 9; 5; 7-2013; 381-390
2047-6310
url http://hdl.handle.net/11336/8522
identifier_str_mv Inzaugarat, Maria Eugenia; Billordo, Luis Ariel; Vodánovich, F.; Cervini Bohm, Gabriela Marta ; Casavalle, Patricia Lucía; et al.; Alterations in innate and adaptive immune leukocytes are involved in paediatric obesity; Wiley; Pediatric Obesity; 9; 5; 7-2013; 381-390
2047-6310
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.2047-6310.2013.00179.x/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.2047-6310.2013.00179.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.885934

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