Determinacion de cromosomas holocineticos a través de la secuencia CID (cenp) en Dermaptera y Neuroptera (Insecta)

Autores
Silenzi Usandivaras, Gabriela Maria; Álvarez, R. E.; Andrada, Aldo Rubén; Oviedo, Andrea Verónica de Fátima; Moreno Ruiz Holgado, Maria Macarena; Ruiz de Bigliardo, Graciela; Romero Sueldo, Gladys Mabel
Año de publicación
2018
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
During cell division, the centromere is an indispensable chromosomal region to ensure the segregation of geneticsinformation. In most eukaryotes, the identity of the centromere is determined by the presence of the CENP protein(CID in Diptera). Some groups of animals have chromosomes that lack localized centromeres (holocentric); a clearexample of this is Dermaptera, although the holocentricity of their chromosomes is questioned since structuressimilar to centromeres were observed in them. It is also believed that neuroptera carries chromosomes with localizedcentromeres (monocentric), but recent studies have questioned this assertion since Chrysoperla presentschromosomes lacking centromeres. The aim of this work was to determine the holocentricity of chromosomes ofDoru linerae, D. luteipes (Dermaptera) and Chrysoperla externa (Neuroptera) by amplifying the CENP gen. Usingthe Quiagen extraction kit, total DNA was obtained fromf Droshophila melanogaster, Zaprionus indianus gupta(Diptera) and Astylus atromaculatus (Coleoptera) species with monocentric chromosomes and the target species. Theprimers used were: Fw-5´GAGAACGGAGCTTGGGTT3´; Rv5´CGTCGGCGAACAACTCAAG3´. PCRamplification was performed with conventional protocols. The final product was identified in 1% agarose gel. ThePCR reaction was positive for Diptera and Coleoptera. The lack of amplicons of CENP gen in Doru and Chrysoperlais another fact that demonstrates the holocentricity of their chromosomes.
Fil: Silenzi Usandivaras, Gabriela Maria. Fundación Miguel Lillo; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Álvarez, R. E.. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Andrada, Aldo Rubén. Fundación Miguel Lillo; Argentina
Fil: Oviedo, Andrea Verónica de Fátima. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Moreno Ruiz Holgado, Maria Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Ruiz de Bigliardo, Graciela. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Fundación Miguel Lillo; Argentina
Fil: Romero Sueldo, Gladys Mabel. Fundación Miguel Lillo; Argentina
XXXV Annual Scientific Meeting of the Tucuman Biology Association
San Miguel de Tucumán
Argentina
Asociación de Biología de Tucumán
Materia
Cromosomas
Dermaptera
Holcineticos
Neuroptera
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/220198
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/220198
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Determinacion de cromosomas holocineticos a través de la secuencia CID (cenp) en Dermaptera y Neuroptera (Insecta)Silenzi Usandivaras, Gabriela MariaÁlvarez, R. E.Andrada, Aldo RubénOviedo, Andrea Verónica de FátimaMoreno Ruiz Holgado, Maria MacarenaRuiz de Bigliardo, GracielaRomero Sueldo, Gladys MabelCromosomasDermapteraHolcineticosNeuropterahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1During cell division, the centromere is an indispensable chromosomal region to ensure the segregation of geneticsinformation. In most eukaryotes, the identity of the centromere is determined by the presence of the CENP protein(CID in Diptera). Some groups of animals have chromosomes that lack localized centromeres (holocentric); a clearexample of this is Dermaptera, although the holocentricity of their chromosomes is questioned since structuressimilar to centromeres were observed in them. It is also believed that neuroptera carries chromosomes with localizedcentromeres (monocentric), but recent studies have questioned this assertion since Chrysoperla presentschromosomes lacking centromeres. The aim of this work was to determine the holocentricity of chromosomes ofDoru linerae, D. luteipes (Dermaptera) and Chrysoperla externa (Neuroptera) by amplifying the CENP gen. Usingthe Quiagen extraction kit, total DNA was obtained fromf Droshophila melanogaster, Zaprionus indianus gupta(Diptera) and Astylus atromaculatus (Coleoptera) species with monocentric chromosomes and the target species. Theprimers used were: Fw-5´GAGAACGGAGCTTGGGTT3´; Rv5´CGTCGGCGAACAACTCAAG3´. PCRamplification was performed with conventional protocols. The final product was identified in 1% agarose gel. ThePCR reaction was positive for Diptera and Coleoptera. The lack of amplicons of CENP gen in Doru and Chrysoperlais another fact that demonstrates the holocentricity of their chromosomes.Fil: Silenzi Usandivaras, Gabriela Maria. Fundación Miguel Lillo; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Álvarez, R. E.. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Andrada, Aldo Rubén. Fundación Miguel Lillo; ArgentinaFil: Oviedo, Andrea Verónica de Fátima. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Moreno Ruiz Holgado, Maria Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Ruiz de Bigliardo, Graciela. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Fundación Miguel Lillo; ArgentinaFil: Romero Sueldo, Gladys Mabel. Fundación Miguel Lillo; ArgentinaXXXV Annual Scientific Meeting of the Tucuman Biology AssociationSan Miguel de TucumánArgentinaAsociación de Biología de TucumánTech Science Press2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/220198Determinacion de cromosomas holocineticos a través de la secuencia CID (cenp) en Dermaptera y Neuroptera (Insecta); XXXV Annual Scientific Meeting of the Tucuman Biology Association; San Miguel de Tucumán; Argentina; 2018; 13-130327-95451667-5746CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/http://www.asobioltuc.com/uploads/archivos/1564748217_QmlvY2VsbCBYWFhWIEpvcm5hZGFzIC0gMjAxOC5wZGY=.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:46Zoai:ri.conicet.gov.ar:11336/220198instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:47.269CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Determinacion de cromosomas holocineticos a través de la secuencia CID (cenp) en Dermaptera y Neuroptera (Insecta)
title Determinacion de cromosomas holocineticos a través de la secuencia CID (cenp) en Dermaptera y Neuroptera (Insecta)
spellingShingle Determinacion de cromosomas holocineticos a través de la secuencia CID (cenp) en Dermaptera y Neuroptera (Insecta)
Silenzi Usandivaras, Gabriela Maria
Cromosomas
Dermaptera
Holcineticos
Neuroptera
title_short Determinacion de cromosomas holocineticos a través de la secuencia CID (cenp) en Dermaptera y Neuroptera (Insecta)
title_full Determinacion de cromosomas holocineticos a través de la secuencia CID (cenp) en Dermaptera y Neuroptera (Insecta)
title_fullStr Determinacion de cromosomas holocineticos a través de la secuencia CID (cenp) en Dermaptera y Neuroptera (Insecta)
title_full_unstemmed Determinacion de cromosomas holocineticos a través de la secuencia CID (cenp) en Dermaptera y Neuroptera (Insecta)
title_sort Determinacion de cromosomas holocineticos a través de la secuencia CID (cenp) en Dermaptera y Neuroptera (Insecta)
dc.creator.none.fl_str_mv Silenzi Usandivaras, Gabriela Maria
Álvarez, R. E.
Andrada, Aldo Rubén
Oviedo, Andrea Verónica de Fátima
Moreno Ruiz Holgado, Maria Macarena
Ruiz de Bigliardo, Graciela
Romero Sueldo, Gladys Mabel
author Silenzi Usandivaras, Gabriela Maria
author_facet Silenzi Usandivaras, Gabriela Maria
Álvarez, R. E.
Andrada, Aldo Rubén
Oviedo, Andrea Verónica de Fátima
Moreno Ruiz Holgado, Maria Macarena
Ruiz de Bigliardo, Graciela
Romero Sueldo, Gladys Mabel
author_role author
author2 Álvarez, R. E.
Andrada, Aldo Rubén
Oviedo, Andrea Verónica de Fátima
Moreno Ruiz Holgado, Maria Macarena
Ruiz de Bigliardo, Graciela
Romero Sueldo, Gladys Mabel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cromosomas
Dermaptera
Holcineticos
Neuroptera
topic Cromosomas
Dermaptera
Holcineticos
Neuroptera
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv During cell division, the centromere is an indispensable chromosomal region to ensure the segregation of geneticsinformation. In most eukaryotes, the identity of the centromere is determined by the presence of the CENP protein(CID in Diptera). Some groups of animals have chromosomes that lack localized centromeres (holocentric); a clearexample of this is Dermaptera, although the holocentricity of their chromosomes is questioned since structuressimilar to centromeres were observed in them. It is also believed that neuroptera carries chromosomes with localizedcentromeres (monocentric), but recent studies have questioned this assertion since Chrysoperla presentschromosomes lacking centromeres. The aim of this work was to determine the holocentricity of chromosomes ofDoru linerae, D. luteipes (Dermaptera) and Chrysoperla externa (Neuroptera) by amplifying the CENP gen. Usingthe Quiagen extraction kit, total DNA was obtained fromf Droshophila melanogaster, Zaprionus indianus gupta(Diptera) and Astylus atromaculatus (Coleoptera) species with monocentric chromosomes and the target species. Theprimers used were: Fw-5´GAGAACGGAGCTTGGGTT3´; Rv5´CGTCGGCGAACAACTCAAG3´. PCRamplification was performed with conventional protocols. The final product was identified in 1% agarose gel. ThePCR reaction was positive for Diptera and Coleoptera. The lack of amplicons of CENP gen in Doru and Chrysoperlais another fact that demonstrates the holocentricity of their chromosomes.
Fil: Silenzi Usandivaras, Gabriela Maria. Fundación Miguel Lillo; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Álvarez, R. E.. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Andrada, Aldo Rubén. Fundación Miguel Lillo; Argentina
Fil: Oviedo, Andrea Verónica de Fátima. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Moreno Ruiz Holgado, Maria Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Ruiz de Bigliardo, Graciela. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Fundación Miguel Lillo; Argentina
Fil: Romero Sueldo, Gladys Mabel. Fundación Miguel Lillo; Argentina
XXXV Annual Scientific Meeting of the Tucuman Biology Association
San Miguel de Tucumán
Argentina
Asociación de Biología de Tucumán
description During cell division, the centromere is an indispensable chromosomal region to ensure the segregation of geneticsinformation. In most eukaryotes, the identity of the centromere is determined by the presence of the CENP protein(CID in Diptera). Some groups of animals have chromosomes that lack localized centromeres (holocentric); a clearexample of this is Dermaptera, although the holocentricity of their chromosomes is questioned since structuressimilar to centromeres were observed in them. It is also believed that neuroptera carries chromosomes with localizedcentromeres (monocentric), but recent studies have questioned this assertion since Chrysoperla presentschromosomes lacking centromeres. The aim of this work was to determine the holocentricity of chromosomes ofDoru linerae, D. luteipes (Dermaptera) and Chrysoperla externa (Neuroptera) by amplifying the CENP gen. Usingthe Quiagen extraction kit, total DNA was obtained fromf Droshophila melanogaster, Zaprionus indianus gupta(Diptera) and Astylus atromaculatus (Coleoptera) species with monocentric chromosomes and the target species. Theprimers used were: Fw-5´GAGAACGGAGCTTGGGTT3´; Rv5´CGTCGGCGAACAACTCAAG3´. PCRamplification was performed with conventional protocols. The final product was identified in 1% agarose gel. ThePCR reaction was positive for Diptera and Coleoptera. The lack of amplicons of CENP gen in Doru and Chrysoperlais another fact that demonstrates the holocentricity of their chromosomes.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/220198
Determinacion de cromosomas holocineticos a través de la secuencia CID (cenp) en Dermaptera y Neuroptera (Insecta); XXXV Annual Scientific Meeting of the Tucuman Biology Association; San Miguel de Tucumán; Argentina; 2018; 13-13
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/220198
identifier_str_mv Determinacion de cromosomas holocineticos a través de la secuencia CID (cenp) en Dermaptera y Neuroptera (Insecta); XXXV Annual Scientific Meeting of the Tucuman Biology Association; San Miguel de Tucumán; Argentina; 2018; 13-13
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.asobioltuc.com/uploads/archivos/1564748217_QmlvY2VsbCBYWFhWIEpvcm5hZGFzIC0gMjAxOC5wZGY=.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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