First identification of nosema ceranae (Microsporidia) infecting apis mellifera in Venezuela

Autores
Porrini, Leonardo Pablo; Porrini, Martín Pablo; Garrido, Paula Melisa; Principal, Judith; Barrios Suarez, Carlos J.; Bianchi, Brigitte; Fernandez Iriarte, Pedro Jose; Eguaras, Martin Javier
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nosema ceranae is a pathogen of Apis mellifera, which seems to have jumped from its original host Asiatic honey bee Apis ceranae. Nosemosis which affects the honey bee Apis mellifera is caused by two parasitic fungi described as etiologic agents of the disease. Nosema apis was the only microsporidian infection identified in A. mellifera until N. ceranae in Taiwan and Europe. Nosema spp. positive samples of adult worker bees from the Venezuelean state of Lara were determined through light microscopy of spores. Samples were then tested to determine Nosema species (N.apis/N.ceranae) using previously reported PCR primers for the 16S rRNA gene. A multiplex PCR assay was used to differentiate both N. apis and N. ceranae species. Only N. ceranae was found in the analyzed samples and the percentage of infected foragers fluctuated between 18% and 60%.
Fil: Porrini, Leonardo Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Porrini, Martín Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Garrido, Paula Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Principal, Judith. Universidad Centro Occidental Lisandro Alvarado; Venezuela
Fil: Barrios Suarez, Carlos J.. Universidad Centro Occidental Lisandro Alvarado; Venezuela
Fil: Bianchi, Brigitte. Universidad Centro Occidental Lisandro Alvarado; Venezuela
Fil: Fernandez Iriarte, Pedro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Materia
HONEYBEE
MICROSPORIDIAN
MULTIPLEX PCR
NOSEMA APIS
NOSEMA CERANAE
VENEZUELA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/77085
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/77085
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First identification of nosema ceranae (Microsporidia) infecting apis mellifera in VenezuelaPorrini, Leonardo PabloPorrini, Martín PabloGarrido, Paula MelisaPrincipal, JudithBarrios Suarez, Carlos J.Bianchi, BrigitteFernandez Iriarte, Pedro JoseEguaras, Martin JavierHONEYBEEMICROSPORIDIANMULTIPLEX PCRNOSEMA APISNOSEMA CERANAEVENEZUELAhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Nosema ceranae is a pathogen of Apis mellifera, which seems to have jumped from its original host Asiatic honey bee Apis ceranae. Nosemosis which affects the honey bee Apis mellifera is caused by two parasitic fungi described as etiologic agents of the disease. Nosema apis was the only microsporidian infection identified in A. mellifera until N. ceranae in Taiwan and Europe. Nosema spp. positive samples of adult worker bees from the Venezuelean state of Lara were determined through light microscopy of spores. Samples were then tested to determine Nosema species (N.apis/N.ceranae) using previously reported PCR primers for the 16S rRNA gene. A multiplex PCR assay was used to differentiate both N. apis and N. ceranae species. Only N. ceranae was found in the analyzed samples and the percentage of infected foragers fluctuated between 18% and 60%.Fil: Porrini, Leonardo Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Porrini, Martín Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Garrido, Paula Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Principal, Judith. Universidad Centro Occidental Lisandro Alvarado; VenezuelaFil: Barrios Suarez, Carlos J.. Universidad Centro Occidental Lisandro Alvarado; VenezuelaFil: Bianchi, Brigitte. Universidad Centro Occidental Lisandro Alvarado; VenezuelaFil: Fernandez Iriarte, Pedro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaResearch Inst Pomology Floriculture2017-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/77085Porrini, Leonardo Pablo; Porrini, Martín Pablo; Garrido, Paula Melisa; Principal, Judith; Barrios Suarez, Carlos J.; et al.; First identification of nosema ceranae (Microsporidia) infecting apis mellifera in Venezuela; Research Inst Pomology Floriculture; Journal Of Apicultural Science; 61; 1; 6-2017; 149-1521643-44392299-4831CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.degruyter.com/view/j/jas.2017.61.issue-1/jas-2017-0010/jas-2017-0010.xmlinfo:eu-repo/semantics/altIdentifier/doi/10.1515/jas-2017-0010info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:24:56Zoai:ri.conicet.gov.ar:11336/77085instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:24:57.285CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First identification of nosema ceranae (Microsporidia) infecting apis mellifera in Venezuela
title First identification of nosema ceranae (Microsporidia) infecting apis mellifera in Venezuela
spellingShingle First identification of nosema ceranae (Microsporidia) infecting apis mellifera in Venezuela
Porrini, Leonardo Pablo
HONEYBEE
MICROSPORIDIAN
MULTIPLEX PCR
NOSEMA APIS
NOSEMA CERANAE
VENEZUELA
title_short First identification of nosema ceranae (Microsporidia) infecting apis mellifera in Venezuela
title_full First identification of nosema ceranae (Microsporidia) infecting apis mellifera in Venezuela
title_fullStr First identification of nosema ceranae (Microsporidia) infecting apis mellifera in Venezuela
title_full_unstemmed First identification of nosema ceranae (Microsporidia) infecting apis mellifera in Venezuela
title_sort First identification of nosema ceranae (Microsporidia) infecting apis mellifera in Venezuela
dc.creator.none.fl_str_mv Porrini, Leonardo Pablo
Porrini, Martín Pablo
Garrido, Paula Melisa
Principal, Judith
Barrios Suarez, Carlos J.
Bianchi, Brigitte
Fernandez Iriarte, Pedro Jose
Eguaras, Martin Javier
author Porrini, Leonardo Pablo
author_facet Porrini, Leonardo Pablo
Porrini, Martín Pablo
Garrido, Paula Melisa
Principal, Judith
Barrios Suarez, Carlos J.
Bianchi, Brigitte
Fernandez Iriarte, Pedro Jose
Eguaras, Martin Javier
author_role author
author2 Porrini, Martín Pablo
Garrido, Paula Melisa
Principal, Judith
Barrios Suarez, Carlos J.
Bianchi, Brigitte
Fernandez Iriarte, Pedro Jose
Eguaras, Martin Javier
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv HONEYBEE
MICROSPORIDIAN
MULTIPLEX PCR
NOSEMA APIS
NOSEMA CERANAE
VENEZUELA
topic HONEYBEE
MICROSPORIDIAN
MULTIPLEX PCR
NOSEMA APIS
NOSEMA CERANAE
VENEZUELA
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Nosema ceranae is a pathogen of Apis mellifera, which seems to have jumped from its original host Asiatic honey bee Apis ceranae. Nosemosis which affects the honey bee Apis mellifera is caused by two parasitic fungi described as etiologic agents of the disease. Nosema apis was the only microsporidian infection identified in A. mellifera until N. ceranae in Taiwan and Europe. Nosema spp. positive samples of adult worker bees from the Venezuelean state of Lara were determined through light microscopy of spores. Samples were then tested to determine Nosema species (N.apis/N.ceranae) using previously reported PCR primers for the 16S rRNA gene. A multiplex PCR assay was used to differentiate both N. apis and N. ceranae species. Only N. ceranae was found in the analyzed samples and the percentage of infected foragers fluctuated between 18% and 60%.
Fil: Porrini, Leonardo Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Porrini, Martín Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Garrido, Paula Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Principal, Judith. Universidad Centro Occidental Lisandro Alvarado; Venezuela
Fil: Barrios Suarez, Carlos J.. Universidad Centro Occidental Lisandro Alvarado; Venezuela
Fil: Bianchi, Brigitte. Universidad Centro Occidental Lisandro Alvarado; Venezuela
Fil: Fernandez Iriarte, Pedro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
description Nosema ceranae is a pathogen of Apis mellifera, which seems to have jumped from its original host Asiatic honey bee Apis ceranae. Nosemosis which affects the honey bee Apis mellifera is caused by two parasitic fungi described as etiologic agents of the disease. Nosema apis was the only microsporidian infection identified in A. mellifera until N. ceranae in Taiwan and Europe. Nosema spp. positive samples of adult worker bees from the Venezuelean state of Lara were determined through light microscopy of spores. Samples were then tested to determine Nosema species (N.apis/N.ceranae) using previously reported PCR primers for the 16S rRNA gene. A multiplex PCR assay was used to differentiate both N. apis and N. ceranae species. Only N. ceranae was found in the analyzed samples and the percentage of infected foragers fluctuated between 18% and 60%.
publishDate 2017
dc.date.none.fl_str_mv 2017-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/77085
Porrini, Leonardo Pablo; Porrini, Martín Pablo; Garrido, Paula Melisa; Principal, Judith; Barrios Suarez, Carlos J.; et al.; First identification of nosema ceranae (Microsporidia) infecting apis mellifera in Venezuela; Research Inst Pomology Floriculture; Journal Of Apicultural Science; 61; 1; 6-2017; 149-152
1643-4439
2299-4831
CONICET Digital
CONICET
url http://hdl.handle.net/11336/77085
identifier_str_mv Porrini, Leonardo Pablo; Porrini, Martín Pablo; Garrido, Paula Melisa; Principal, Judith; Barrios Suarez, Carlos J.; et al.; First identification of nosema ceranae (Microsporidia) infecting apis mellifera in Venezuela; Research Inst Pomology Floriculture; Journal Of Apicultural Science; 61; 1; 6-2017; 149-152
1643-4439
2299-4831
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.degruyter.com/view/j/jas.2017.61.issue-1/jas-2017-0010/jas-2017-0010.xml
info:eu-repo/semantics/altIdentifier/doi/10.1515/jas-2017-0010
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Research Inst Pomology Floriculture
publisher.none.fl_str_mv Research Inst Pomology Floriculture
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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